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MCP-1에 의해 유도된 THP-1 유주에 미치는 2-methoxy-1,4-naphthoquinone (MQ)의 영향
김시현,박보빈,홍성은,유성률,이장호,김사현,이평재,조은경,문철,Kim, Si Hyun,Park, Bo Bin,Hong, Sung Eun,Ryu, Sung Ryul,Lee, Jang Ho,Kim, Sa Hyun,Lee, Pyeongjae,Cho, Eun-Kyung,Moon, Cheol 대한임상검사과학회 2019 대한임상검사과학회지(KJCLS) Vol.51 No.2
본 연구는 2-methoxy-1,4-naphthoquinone (MQ)이 Monocyte chemoattractant protein-1 (MCP-1)에 의해 유도된 단구 유주에 미치는 효과를 알아보고자 진행되었다. 단구의 유주(migration) 현상은 신체 방어와 면역반응에 중요한 현상이다. MQ는 아시아권 국가에서 다양한 질병과 통증 치료에 민간요법으로 오랫동안 사용되었던 봉선화(Impatiens balsamina) 잎으로부터 추출한 주요 성분이다. 단구 세포주 THP-1를 이용하여 실험을 진행하였으며, MQ의 세포독성, MCP-1에 의해 유도된 유주 현상에 미치는 영향을 transwell system을 이용하여 확인하였다. MQ 작용기전을 이해하기 위해 cAMP 발현 및 Erk1/2 인산화를 각각 ELISA, Western-blot 기법을 통해 분석하였다. MQ의 단구 세포주 THP-1에 대한 MQ의 세포독성은 $10{\mu}M$ 농도에서 나타나기 시작했으며, $100{\mu}M$ 농도에서 약 50% 가량의 세포독성을 확인했다. 염증성 화학주성 인자 MCP-1에 의해 유도된 단핵구 세포주 THP-1의 유주현상은 MQ 처리 후 농도증가에 비례하여 증가하였으며, $0.1{\mu}M$ 농도의 MQ를 처리했을 때 가장 높은 증가를 보였다. MCP-1 단독 처리 시 감소하는 cAMP 의 배양액 내 발현은 MQ 동시 처리 시 더욱 감소하였고, 유주현상과 마찬가지로 $0.1{\mu}M$ 농도에서 가장 감소하였다. MCP-1의 수용체인 C-C motif chemokine receptor 2 (CCR2) 신호전달 과정에 관여하는 주요 신호전달 단백질인 Erk1/2의 인산화도 $0.1{\mu}M$ MQ 동시 처리 시 증가하였다. 이상의 결과를 통해 MQ가 MCP-1에 의해 유도되는 THP-1 세포주의 유주현상을 증가시키며, 연관된 cAMP 발현을 감소, Erk1/2 인산화는 증가시키는 경향을 확인하였다. This study examined the effects of 2-methoxy-1,4-naphthoquinone (MQ) on the monocyte chemoattractant protein-1 (MCP-1)-induced migration of monocytes, which is an important phenomenon for the body defense and immune response. MQ is a major component extracted from Impatiens balsamina leaves, which have been used for many years in Asian medicine for the treatment of a range of diseases and pain. The cytotoxicity of MQ began to appear at a concentration of $10{\mu}M$, and approximately 50% cytotoxicity was confirmed at $100{\mu}M$. The MCP-1 induced migration of the THP-1 monocyte cell line increased after MQ treatment in a dose dependent manner and the largest increase was observed at $0.1{\mu}M$. The level of cAMP expression decreased after a treatment with $0.1{\mu}M$ MQ. The phosphorylation of extracellular signal-regulated kinases 1/2 (Erk1/2), a key signaling protein involved in the signaling pathway of C-C motif chemokine receptor 2 (CCR2), a receptor for MCP-1, was increased by the simultaneous treatment of $0.1{\mu}M$ MQ. These results show that MQ increases the MCP-1-induced migration of THP-1, decreases the level of cAMP expression, and increases the level of Erk1/2 phosphorylation.
White Oganic Light-Emitting Diodes based on Simply Modified Anthracene and Rubrene
김시현,이승희,Kim, Si-Hyun,Lee, Seung-Hee The Korean Society of Applied Science and Technolo 2022 한국응용과학기술학회지 Vol.39 No.5
The white OLED is fabricated with the anthracene-based blue emitting material, 9-(2-naphthyl)-10-(p-tolyl)anthracene (2-NTA) in various volume-ratios of orange dopant, rubrene, which results in pure white emission with C.I.E. coordinate of ~(0.32, 0.39). The devices with <1.5% rubrene show better EL properties (efficiency) than >3% devices. Furthermore the turn-on voltage of 2-NTA WOLED (3.7 V) is lower than that of 2-NTA blue OLED (5.4 V) at the same condition. Conclusively 2-NTA with rubrene less than 1.5% (v/v) could be utilized for the pure WOLED.
타인 동종 말초혈액 조혈모세포이식 후 발생한 공기-누출 증후군 1예
김시현 ( Si Hyun Kim ),신주영 ( Ju Young Shin ),황현석 ( Hyun Suk Hwang ),김대준 ( Dae Jun Kim ),김희제 ( Hee Je Kim ),민우성 ( Woo Sung Min ),김춘추 ( Chun Choo Kim ) 대한내과학회 2008 대한내과학회지 Vol.74 No.4
Bronchiolitis obliterans, a late, non-infectious pulmonary complication of bone marrow transplantation (BMT), is an obstructive airway disease associated with chronic graft-versus-host disease (cGVHD). We report a rare case of air-leakage syndrome including pneumothoraces, pneumomediastinum, and subcutaneous emphysema with bronchiolitis obliterans after allogeneic peripheral blood stem cell transplantation (PBSCT) from an unrelated donor. The pathogenesis of air-leakage syndrome is not fully understood, and its management has not yet been established. This patient was treated with high-concentration oxygen, tube thoracostomy, percutaneous drainage with a pigtail catheter to relieve the tension pneumomediastinum, and mechanical ventilatory support. The patient has now recovered and is being followed on an outpatient basis.(Korean J Med 74:445-450, 2008)
공동주택의 에너지 성능 최적화를 위한 개방유형별 외피성능
김시현(Kim Si-Hyun),임도요(Lim Do-Yo),정창헌(Cheong Chang-Heon),김태언(Kim Tae-Yeon),이승복(Leigh Seung-Bok) 대한건축학회 2008 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.28 No.1(계획계)
This study proposed guidelines of envelope thermal performance to optimize energy performance in apartment houses. Energy reduction goal which is first grade in energy performance level is reducing heating load 33.5% in comparison with standard house. As the results of the envelope performance depending on opening type, envelope performance is great from three sides opening than from both faces opening, right triangle opening. The analyzing envelope performance about changing S/V ratio range from 20 to 10, performance improve U-value of external wall from 0.47W/㎡K to 0.4W/㎡K and U-value of window from 3.4W/㎡K to 2.22~2.8W/㎡K.
아버지의 언어통제유형과 유아의 사회성 및 부적응 행동과의 관계
김시현(Si Hyun Kim),김화수(Wha Soo Kim) 한국언어치료학회 2016 한국언어치료학회 학술발표대회 논문집 Vol.2016 No.12
Purpose: This study focused on the father’s language control type to young children’s behavior, and the relationship between young children’s sociality, maladjustment behavior. Methods: For this study, “Parents’ Language Control Type Scale” and “Korean-Child Development Inventory” was used. This study was proceeded with 2 to 5 year-old children, each 70 of their fathers, total 140 subjects. Results: first, “position-oriented” type of father’s language control type is shown the highest. “Person-oriented” type, “imperative-oriented” type followed in order. Second, father’s language control type and young children’s sociality appeared to be significant correlation. Also it appeared that the higher father"s “imperative-oriented” type, the higher young children’s maladjustment behavior. conclusion: Additionally the higher fathers’ “person-oriented” type, the lower young children’s maladjustment behavior.