여성 생식기 암에서 c-K-ras 암유전자의 점 돌연변이 검출

김승조,이필호,박종섭,남궁성은,김휘,고영미,김홍기,한상균 대한부인종양 콜포스코피학회 1994 Journal of Gynecologic Oncology Vol.5 No.2

lt has been well established that, specifi alterations in members of the ras gene family, H-ras, K-ras and N-ras, can convert them into active oncogenes. These alterations are either point mutations occurirg in either codon 12, 13 or 61, or alternatively, a 5- to 50-fold amplification of the wfld-type gene. Activated ras oncogenes have been found in a significant proportion of all turnors, but the incidence varies considerably with the tumor type : it is frequent (20~40% ) in colarectal eancer and acute myeloid leukemia, but absent or preaent rarely in breast and atomach cancer. But the role of c-K-ras point mutatio in the development of cancers in the female genital tract has not been extensively studied. Polymerase chain reaction followed by gel electrophoresis was performed respectively using wild-type normal and specific point mutation primers{GGT-$gt;GAT, GGT-$gt;AGT, GGT-$gt;TGT and GGT-$gt;GTT) to detect, point, mutation of codon 12 of c-K-ras oncogene. The c-K-ras oncogene point mutation was confirmed by Southern blot hybridization using synthetic oligonucleatide probe. 3'-end Iabelled with digoxigenin -dUTP. With this method, the frequency of point mutation on codon 12 of c-K- ras oncogene was examined the tissues in 37 casea of ovarian cancer, 7 cases of endometrial cancer, 36 cases of the gestational trophoblastic tumor, 60 cases of cervicaI cancer. The relationship between the presence of a c-K-ras point mutation and clinicppathologi al characteristics of the female genetial tract cancers were also analysed The results were as follows; 1. The incidence of four point mutations on codon 12 of K-ras oncogene in 37 ovarian cancers was 45.9% (17/37) and distribution were 43.2%(16/37), 2.7%(1/37), 0%(0/37) and 0%(0/37) in GGT�GAT, GGT�AGT, GGT�TGT, and GGT�GTT, respectively According to histological type, in ovarian cancers, the point mutation of K-ras oncogene waspositive in 45% (10/22) of serous cystadenocarcinomas. The incidence of four point mutations on codon 12 among 37 patients with ovarian cancer according to histological type was 45.5% (10?22) with serous cystadenocarcinoma, 57.1% (4/7) of mucinous cystadenocarcinoma. Comparing the positive rate of point mutations of K-ras oncogene among 37 patients with ovarian cancer with the clinical stage, point mutation was detected in 28.5%(2/7) or patients with stage, I. 40.0%(2/5) with stage II. and 52.0%(13\/25) with stage III/IV, there was no statistically significant increasement of point mutaiions with the advance of the clinical stage of ovarian cancer. Comparing the positive rate of point mutations of K-ras oncogen among 37 patients with ovarian cancer according to the histologic grade, point mutation was detected in 50.0%(2/4) of patients with grade I, 41.7%(5/12) with grade II and 47.6%(10/21) with grade III. 2. The incidence of point mutations of K-ras oncogen among 33 patients with ovarian cancer who were performed pelvic lymph nocd dissection was 57.15, (12/21) of the patients with pelvic lymph node metastases and 16.7%(2/12) of the patients without pelvic lymph node metastases. There was statistically significant difference between the positive rate of c-K-ras point mutations and the pelvic lymph nodal status(P$lt;0.05). 3. In 7 cases of endometrial cnacer, poistive rate of K-ras point mutation was 42.8% (3/7). point mutations were also detected in 2 cases from 4 choriocarcinomas, but, the point mutation was only detected in 1 case from 60 cervical carcinomas From thses results, we may suggest that the point mutation on codon 12 of c-K-ras oncogene are considered to be one of the important genetic change in the tumor formation and progression of ovarian cancers. the activation of c-K-ras oncogene seems to be the on step in the multistep process of tumor formation in ovarian cancer, furthermore, the point mutation of c-K-ras gene could occur more frequently in the patients of ovarian cancer with plevic lymph node metastases than in those without pelvic metatases, suggesting the role in tumor progression. And we concluded that point mutation on codon 12 is comparable frequent in uterine endometrial carcinomas and may have significance as an event that contributes to progrrssion of endometrial cancers and choriocarcinoma, but cervical carcinoma do not appear to have c-K-ras point mutation in general. More studies will be necessary, but the detection of c-K-ras point mutationas the possibility of biological tumor marker to predict clinical outcome may be utilized in female malignancies

巨大卵巢襄腫을 同伴한 仰臥位血壓症候群의 1例

金丞兆,金星圭,吳慶根 大韓産婦人科學會 1965 大韓産婦人科學會雜誌 Vol.8 No.1

리팍시민이 흰쥐의 의액분비 및 생쥐의 소장수송능에 미치는 영향

서희경,태윤식,강시현,이현정,이현화,김승조,황성주 충남대학교 약학대학 의약품개발연구소 1994 藥學論文集 Vol.10 No.-

Rifaximin, the active ingredient in Normix??, has an extremely broad spectrum of action covering all intestinal germs and its absorption is practically zero. It is not appreciably absorbed through the gastrointestinal(GI) tract and not inactivated by gastric juice and is eliminated unchanged in stools. General pharmacology of rifaximin has not been studied sufficiently due to its negligible absorption. In this study, the effect of rifaximin on the gastric juice secretion was investigated in rats and its effect on the gastrointestinal motility was also investigated in mice. Rifaximin had no effect on pH, volume, acidity and pepsin activity of gastric juice in rats and also no effect on GI motility(charcoal transport) in mice. Therefore, rifaximin may have little effect on the GI tract.

인터넷 수퍼컴퓨팅 기술의 구현

김승조(Seung Jo Kim),이창성(Chang Sung Lee),손정우(Jung-Woo Sohn),하병언(Byung un Ha),조진연(Jin Yeon Cho) 한국항공우주학회 2001 韓國航空宇宙學會誌 Vol.29 No.3

항공기 구조 발달사-제2부

김승조(Seung Jo Kim),박성호(Sung Ho Park) 한국항공우주학회 1999 韓國航空宇宙學會誌 Vol.27 No.3

REALIZATION OF INTERNET PARALLEL SUPERCOMPUTING FOR LARGE-SCALE STRUCTRAL ANALYSIS

Kim, Seung Jo,Lee, Chang Sung,Ha, Byung Un,Choi, Ji Duck,Cho, Jin Yeon,Kim, Jeong Ho 서울대학교 항공우주신기술연구소 2001 항공우주신기술연구소 연구보고 Vol.2 No.2

In this work, Internet Supercomputing methodology is introduced and the concept is materialized for large-scale finite element analysis. The primary resources of Internet Supercomputing are numerous idling PCs connected by Internet with no regards to their locations. Under the above concept, virtual supercomputing system InterSup I is constructed and tested. To establish the InterSup I system, 64 PC nodes, which are located in several places and connected by Internet, are conscripted, and the finite element code, IPSAP(Internet Parallel Structural Analysis Program) based on parallel multifrontal solver is developed for solving structural analysis problems. And mesh-partitioner based on graph-partitioning algorithms with WVEM(Weight Vertex and Edge Method) proposed in this work also developed. By the establish InterSup I system, analysis of finite element structural model having around 10 million DOFs are solved and the elapse time is 28,194 sec. From these results, it is confirmed that the proposed Internet Supercomputing methodology has a great promise and potential as a next-generation.

항공기 구조 발달사-제3부

김승조(Seung Jo Kim),박성호(Sung Ho Park) 한국항공우주학회 1999 韓國航空宇宙學會誌 Vol.27 No.4

염산, 무기질 및 DHA를 보충한 식이가 흰쥐의 출산능력과 어린 쥐의 학습능력에 미치는 영향

김승조 ( Seong Jo Kim ),하태열 ( Tae Yel Ha ),한찬규 ( Chan Kyu Han ),김나영 ( Na Young Kim ),안홍석 ( Hong Seok Ahn ),신현경 ( Hyeon Kyeong Shin ),신승주 ( Seong Ju Shin ),장성운 ( Sung Woon Chang ),이정노 ( Jung Noh Lee ),임준규 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.9

목적 : 영양부족이나 영양장애는 임신에 문제를 일으킬 수 있으며, 임신기간 동안 적절한 영양 섭취가 모체의 건강과 태아의 성장발달에 지대한 영향을 미친다. 그러나 여성에서 미량영양소와 임신율 등 생식능력과의 관련성에 관한 보고는 매우 드문 실정이다. 본 연구는 엽산, 아연, 철분, 칼슘, DHA 등을 첨가한 영양보충식이 암컷 흰쥐의 생식 능력과 임신결과 및 나아가 새끼 쥐의 성장발육과 학습능력에 미치는 영향을 알아보고자 하였다. 연구 방법 : 4주령의 암컷 쥐 60마리를 각각 대조군과 영양보충식군으로 나누어, 각각의 실험식이를 공급하였고, 수컷 쥐 20마리는 일반고형사료를 주었다. 사유 2주 후 암컷 쥐와 수컷 쥐를 교미시킨 후 약 3주 후부터 분만이 시작되었으며, 출생 후 새끼 쥐의 성별 구분 및 출생체중을 측정한 뒤 사육 3주 후부터 수미로 테스트를 실시하였다. 분만한 어미 쥐는 실험식이로 4주간 더 사육한 뒤 황체호르몬, 에스트라디올, 난포수, 황체수 등을 측정하여 실험군과 대조군을 비교하였다. 결과 : 영양보충식군에서 암컷 쥐는 대조군의 2배로 30마리 중 22마리가 분만에 성공하였으며 두 군 모두 분만상태는 양호하였다. 두 군의 암컷 쥐들로부터 태어난 새끼 쥐에서는 체중증가의 차이는 없었으나 수미로에서의 유영시간, 오반응수를 측정한 결과, 영양보충식군이 대조군보다 유영시간이 짧았고, 오반응수도 적은 것으로 나타났다. 결론 : 엽산, 아연, DHA 및 타우린 등의 영양보충은 모체의 임신율을 높이고, 태아의 학습능력 향상에 기여하는 것으로 사료된다. 또한 본 연구의 결과는 여성에 있어서 영양과 임신과의 관련성을 연구하는데 기초자료가 될 것으로 기대한다. Objective : Malnutrition and nutritional disorder may cause problem of fertility and therefore adequate nutrition is very important during pregnancy. In this study, we investigated effects of supplemental diet contained folic acid, zinc, calcium, Iron, DHA and taurine on fertility outcome in the female rats and learning ability of their offsprings. Methods : The female rats at 4 week were fed by two group divided control (AIN-76 diet) and supplement diet. The male rats were taken pellet type diet. After 3weeks, female rats and male rats were mated. Then, at 3 weeks after mating, parturition was begun. After paturition, sex and birth weight of offsprings were examined for their offsprings. When the offsprings were 3 weeks of age, position reversional test in a water maze was done for 4 weeks. After female rats were fed experimental diet for 4 weeks, their follicle, corpus luteum, corpus albicans, progesterone, estradiol and ovary weight were measured. Results : 22 rats of 30 in supplemental diet group succeeded on parturition, and 11 rats of 30 in control group succeeded. Pregnancy outcome was fine in both group. There was no significant difference in weight of major bowels and femur length of their offspring. The position reversional test of offspreings in a water maze showed a significant difference between control group and supplement group. Elapsed time and erromeous response to reach the escape platform were significantly lowered in supplemental group than control group. Conclusion : This result suggest that supplementation contained folic acid, multivitamins, DHA and taurine may increase fertility rate in the maternal rats and also learning ability in offsprings.

Supercomputing by Internet Based PCs

Kim, Seung-Jo,Cho, Jin-Yeon,Lee, Chang-Sung,Cho, Chul-Hoon,Ji, Kuk-Hyun 서울대학교 항공우주신기술연구소 2000 항공우주신기술연구소 연구보고 Vol.1 No.2

In this work, Internet Supercomputing methodology is introduced and the concept is realized for large-scale finite element analysis. The primary resources of Supercomputing by Internet based PCs are numerous idling PCs connected through Internet with no regards to their locations. The computing ability of hundreds or thousands PCs networked by Internet can be as powerful as that of supercomputer such as CRAY T3E if these PCs are utilized for solving a problem simultaneously through an efficient parallel computing algorithm. Under the above concept, a virtual supercomputing system InterSup I is constructed and tested. To establish the InterSup I system, 64 PC nodes, which are located in several places and connected by Internet, are conscripted. By the established InterSup I system, linear static analyses of finite element model having around five million DOFs are solved through the parallel multifrontal solver and the time dependent behaviors of structures are investigated with parallel explicit algorithm. Also, the eigen value analyses are conducted through the block Lanczos procedure and parallel multifrontal technique. From these supercomputing results, Supercomputing by Internet based PCs can be considered as one of the cheapest ways as well as one of the most powerful ways to high performance computing (HPC) such as large-scale finite element analysis.

자궁경부암을 유발하는 HPV E6 단백질에 의한 p73 단백질의 불활성화 : p53과 무관한 E6의 새로운 기능

남궁성은(Sung Eun Namkoong),김승조(Seung Jo Kim),김은주(Eun Joo Kim),엄수종(Soo Jong Um),박종섭(Jong Sup Park) 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.11

N/A Objective: Human papillomavirus (HPV) is strongly implicated as a causative agent in the etiology of cervical cancer. Of its gene products, E6 and E7 oncoproteins play major roles by inactivation of cellular p53 and pRb tumor suppressor proteins, respectively. However, it has been recently suggested that p53 and/or pRb-independent functions of E6 and E7 are involved in cervical carcinogenesis. The purpose of this study is to identify novel a cellular target, p73, of E6 and to determine how E6 inactivates p73 function, Methods: The interaction between E6 and p73 were identified by the yeast two-hybrid assay in vivo and the GST pull-down assay in vitro. The function of the interaction was determined by transient transfections using p21 promoter-CAT reporter plasmid. The molecular mechanism underlying the functional significance of the interaction was further assessed by in vivo and in vitro protein degradation assays, and gel mobility shift assays. Results: Yeast two-hybrid and GST pull-down assays indicate a physical interaction between p73 and either HPV-16 or HPV-11 E6 proteins in vivo and in vitro, respectively. Transactivation domain (amino acid residues 1-49) is found to be absolutely required for this interaction. Transient co-expression of E6 significantly inhibits the p73-mediated activation of p21 promoter in a p53-defective C33A cell line. Using Ga14-p73 fusion protein, we demonstrate that E6 inhibition of p73 transactivation function is independent of sequence-specific DNA binding, which is confirmed by direct electrophoretic mobility shift assay. Moreover, E6 inhibits p73 function by interfering with the activity of the amino-terminal activation domain. The protein degradation assays in vivo and in vitro indicate that p73, unlike p53, is not susceptible to E6-dependent proteolysis. Conclusion: Throughout this study, we identified p73 as a novel cellular target of HPV-E6 protein and found that E6 binds p73 through the amino-terminal transactivation domain, and inhibits its transactivation function independent of the protein degradation and DNA binding. These overall results, consequently, suggest that in addition to the inactivation of p53, the functional interference of p73 by HPV-E6 may, at least in part, contribute to E6-mediated cellular transformation.