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Tamoxifen과 17 -estradiol이 시험관내 자궁경부암 및 난소암세포의 성장에 미치는 영향
김승조,김진우,남궁성은,김대훈,김도강,노덕영,박래옥 대한산부인과학회 1991 Obstetrics & Gynecology Science Vol.34 No.1
본 연구는 자궁경부암과 난소암으로부터 적출한 종양세포를 연성 한천세포배양법을 이용하여 배양시킨 후 10-8M, 10-9M 및 10-10M의 17 -estradiol과 10-6M, 10-7M 및 10-8M의 tamoxifen을 배양액에 투여하여 종양세포의 군락형성에 17 -estradiol과 tamoxifen이 미치는 영향을 관찰하여 다음과 같은 결론을 얻었다. 1. 자궁경부암과 난소암의 종양세포 군락형성에 있어서 17 -estradiol의 촉진작용은 없었다. 2. 자궁경부암에서 tamoxifen의 종양세포 군락형성 억제효과는 발견할 수 없었다. 3. 난소암에서 tamoxifen(10-7M&10-8M)은 종양세포 군락형성에 억제효과를 나타내었다. 4. 난소암에서 tamoxifen(10-7M & 10-8M)은 종양 세포 군락형성 억제효과는 17B-estradiol(10-8M)에 의하여 중화되지 않았다.(p$lt;0.03). To evaluate the effect of tamoxifen(TAM) and 17 -estradiol(estradiol) on the growth of cervical and ovarian cancer, the colony growth of cells disaggregated from 5 squamous cell carcinomas of cervix and 6 epithelial ovarian carcinomas in soft agar were studied. Mechanical and enzymatic dispersion was used to prepare a single cell suspension for plating. Tumor cells were cultured in the two-layer of soft agar system with enriched medium. The effect of tamoxifen and estradiol alone or simultaneously on cell growth were assessed. The results were obtained as follows. 1. Colony formation was much increased in serum containing media as compared to serum free media in cervical carcinoma(P$lt;0.015)and ovarian carcinoma(P$lt;0.03, one tailed test). 2. Tstradiol showed no difference on colony formation in cervical and ovarian carcinoma as compared to control value.But in serum free mediym, estradiol(10-8 M)moderately stimulated colony formation of 4 among 6 ovarian carcinomas, while tamoxifen(10-7 M)showed significant inhibition on colony formation of 4 among 6 ovarian carcnomas, while tamoxifen (10-7 M) showed significant inhibition on colony formation(P$lt;0.03). 3. Tamoxifen(10-7 M& 10-8 M) showed significant inhibitory effect on colony formation in ovarian carcinoma(p$lt;0.03), but no inhibition in cervical carcinoma. 4. The addition of estradiol(10-8 M) did not neutralize the inhibitory effect of tamoxifen(10-8 M)on colony formation in ovarian carcinomas(P$lt;0.03).