비소세포성 폐암으로 인한 기관지 림프절 누공

김서우 ( Seo Woo Kim ),김현경 ( Hyun Kyung Kim ),전승정 ( Sung Joung Jeun ),박혜성 ( Hye Sung Park ),장중현 ( Jung Hyun Jang ),이진화 ( Jin Hwa Lee ),류연주 ( Yon Ju Ryu ),심성신 ( Sung Shin Sim ),천은미 ( Eun Mi Chun ) 대한결핵 및 호흡기학회 2010 Tuberculosis and Respiratory Diseases Vol.68 No.4

Lymphadenopathy in the thoracic cavity is frequently caused by inflammatory diseases. In very rare cases, the node-bronchial fistula has been reported to be the cause of complications of pulmonary tuberculosis. A male patient with necrotizing pneumonia and mediastinal lymph node enlargements identified by chest computed tomography was also found to have a node-bronchial fistula caused by lung cancer. The patient was treated for tuberculosis with pneumonia for one week before a definitive diagnosis was made. A further investigation revealed him to have non-small cell lung cancer (NSCLC, adenocarcinoma) and multiple mediastinal lymphadenopathies accompanied with the node-bronchial fistula. We report this specific case that had been previously treated for tuberculosis but was later revealed to be NSCLC accompanied with a node-bronchial fistula.

저가의 머신 비전 카메라를 이용한 2차원 진동의 측정 및 교정

김서우,이정권,Kim, Seo Woo,Ih, Jeong-Guon 한국음향학회 2018 韓國音響學會誌 Vol.37 No.2

현존하는 진동 측정 센서는 정밀도 면에서는 대부분의 진동을 측정하기에 충분하나, 센서 한 개로 하나의 지점이나 방향에 한정하여 측정할 수밖에 없다는 단점을 갖고 있다. 반면 카메라의 경우, 정밀도나 측정 가능한 주파수 영역의 면에서는 다소 불리하지만, 한 번에 광범위한 영역의 진동을 측정할 수 있고 가격 면에서 유리하며 다자유도의 진동을 동시에 측정할 수 있다는 큰 장점을 갖고 있다. 본 연구에서는 저가의 머신 비전 카메라가 진동 측정 센서로서 어느 정도의 오차 범위 내에서 진동을 측정할 수 있는지 알아본 후, 실제 외팔보의 진동을 측정하였다. 카메라의 2차원 평면 이미지는 두 방향의 직선 운동과 한 방향의 회전 운동을 나타낼 수 있다. 먼저 단일 점의 진동을 카메라로 측정하고, LDV(Laser Doppler Vibrometer) 측정을 기준으로 한 카메라 측정의 오차를 실험적으로 교정하였다. 다음으로 다중점의 진동을 한 번에 측정하여 회전 진동과 외팔보 전체 형상의 진동을 측정하였다. 외팔보 전체 형상 진동은 주파수와 시간 영역 모두에 대하여 분석하였다. The precision of the vibration-sensors, contact or non-contact types, is usually satisfactory for the practical measurement applications, but a sensor is confined to the measurement of a point or a direction. Although the precision and frequency span of the low-cost camera are inferior to these sensors, it has the merits in the cost and in the capability of simultaneous measurement of a large vibrating area. Furthermore, a camera can measure multi-degrees-of-freedom of a vibrating object simultaneously. In this study, the calibration method and the dynamic characteristics of the low-cost machine vision camera as a sensor are studied with a demonstrating example of the two-dimensional vibration of a cantilever beam. The planar image of the camera shot reveals two rectilinear and one rotational motion. The rectilinear vibration motion of a single point is first measured using a camera and the camera is experimentally calibrated by calculating error referencing the LDV (Laser Doppler Vibrometer) measurement. Then, by measuring the motion of multiple points at once, the rotational vibration motion and the whole vibration motion of the cantilever beam are measured. The whole vibration motion of the cantilever beam is analyzed both in time and frequency domain.

자기장 지도를 이용한 위치 추정

김한솔(Han-Sol Kim),문우성(Woo-Sung Moon),서우진(Woo-Jin Seo),백광렬(Kwang-Ryul Baek) 제어로봇시스템학회 2013 제어·로봇·시스템학회 논문지 Vol.19 No.4

Geomagnetic is refracted by building’s wall and pillar. Therefore refracted geomagnetic is able to be used as feature point. In a specific space, a mobile device that is equipped with magnetic sensor array measures 3-axis magnetic field for each point. Magnetic field map is acquired by collecting the every sample point in the magnetic field. The measured magnetic field must be calibrated, because each magnetic sensor has a distortion. For this reason, sensor distortion model and sensor calibration method are proposed in this paper. Magnetic field that is measured by mobile device matches magnetic field map. Result of the matching is used for position estimation. This paper implements hardware system for position estimation method using magnetic field map.

Streptavidin이 융합된 DR4 항원에 특이적인 single-chain Fv 항체의 개발

김서우,우상욱,김진규,Kim, Seo Woo,Wu, Sangwook,Kim, Jin-Kyoo 한국미생물학회 2018 미생물학회지 Vol.54 No.4

Streptavidin (STR)과 Biotin system은 Biotin의 Streptavidin에 대한 높은 비공유 친화력(non-covalent affinity; $K_D=10^{-14}M$)과 4 Biotin 결합부위를 갖는 Streptavidin의 tetramer 구조로 인해 복수의 항원결합부위 및 복수의 항원특이성을 갖는 항체를 제조할 수 있기 때문에 가장 활발하게 연구되고 있다. 이 system을 활용하기 위해 우리는 Streptomyces avidinii 염색체 DNA로부터 PCR을 통해 Streptavidin (STR) 유전자를 증폭하고 이를 TRAIL (tumor necrosis factor ${\alpha}$ related apoptosis induced ligand) receptor인 death receptor 4 (DR4)에 특이적으로 결합하는 hAY4 single-chain Fv 항체유전자에 융합시켰다. 대장균에서 발현시킨 STR에 융합된 hAY4 ScFv (hAY4-STR) 항체는 가열시킨 SDS-PAGE에서 43 kDa monomer를 나타내었다. 그러나 가열하지 않은 SDS-PAGE와 Size-exclusion chromatography에서는 tetramer인 172 kDa을 나타내었는데 이는 hAY4 ScFv-STR 항체가 STR의 자연적인 비공유결합에 의해 유도된 tetramer를 형성하고 있음을 나타내고 있다. 본 융합 단백질은 Ouchterlony assay와 ELISA에서 보여주는 것처럼 자연 Streptavidin과 유사한 Biotin 결합력을 유지하고 있었다. ELISA와 Westernblot을 이용하여 정제된 hAY4-STR 융합항체의 DR4 항원결합력 또한 확인하였다. 게다가 표면 플라즈몬 공명(surface plasmon resonance) 분석에서 hAY4 ScFv-STR tetramer는 tetramerization에 의해 hAY4 ScFv monomer보다 60배 더 높은 항원결합력을 나타내었다. 요약하면 hAY4 ScFv-STR 융합단백질은 E. coli에서 soluble tetramer로 성공적으로 발현 및 정제되었으며 Biotin과 DR4 항원에 동시에 결합함을 보여 주었다. 이는 bifunctional and tetrameric ScFv 항체를 제조 할 수 있음을 제시해 주고 있다. The Streptavidin and Biotin system has been studied most extensively as the high affinity non-covalent binding of Biotin to STR ($K_D=10^{-14}M$) and four Biotin binding sites in tetrameric Streptavidin makes this system useful for the production of multivalent antibody. For the application of this system, we cloned Streptavidin amplified from Streptomyces avidinii chromosome by PCR and fused to gene of hAY4 single-chain Fv antibody specific to death receptor 4 (DR4) which is a receptor for tumor necrosis factor ${\alpha}$ related apoptosis induced ligand. The hAY4 single-chain Fv antibody fused to Streptavidin expressed in Escherichia coli showed 43 kDa monomer in heated SDS-PAGE. However, this fusion protein shown in both non-heated SDS-PAGE and Size-exclusion chromatography exhibited 172 kDa as a tetramer suggesting that natural tetramerization of Streptavidin by non-covalent association induced hAY4 single-chain Fv tetramerization. This fusion protein retained a Biotin binding activity similar to natural Streptavidin as shown in Ouchterlony assay and ELISA. Death receptor 4 antigen binding activity of purified hAY4 single-chain Fv fused to Streptavidin was also confirmed by ELISA and Westernblot. In addition, surface plasmon resonance analysis showed 60-fold higher antigen binding affinity of the hAY4-STR than monomeric hAY4 ScFv due to tetramerization. In summary, hAY4 single-chain Fv fused to Streptavidin fusion protein was successfully expressed and purified as a soluble tetramer in E. coli and showed both Biotin and DR4 antigen binding activity suggesting possible production of bifunctional and tetrameric ScFv antibody.

우울증의 병태생리에 관한 동서의학적 비교

오현묵,김서우,오영택,손창규,이진석,Oh, Hyeon-Muk,Kim, Seo-Woo,Oh, Young-Taeck,Son, Chang-Gue,Lee, Jin-Seok 대전대학교 한의학연구소 2017 혜화의학회지 Vol.26 No.1

Objective: This study aims to summarize recent findings of depression and compare the physiopathology of depression by conventional medicine and Korean medicine. Methods: A literature search of PubMed and Journal of Oriental Neuropsychiatry was conducted. This study analyzes the physiopathology of depression. Results: Despite difference of the viewpoints between conventional medicine and Korean medicine, etiology and symptoms of depression are similar. Recent findings of depression partly accord with Korean medicine theory. Conclusion: Physiopathology of depression has yet to be completely understood and current treatments remain ineffective in a large subset of patients. As the efficacy of Korean medicine against depression is demonstrating recently, understanding the viewpoints of Korean medicine will provide new perspectives and hints for further research and therapeutic development.

오가피(Acanthopanax sessiliflorus Seeman) 열매로부터 분리한 페놀 화합물의 항산화활성

인서지 ( Seo Ji In ),이대영 ( Dae Young Lee ),서경화 ( Kyeong Hwa Seo ),남태규 ( Tae Gyu Nam ),김대옥 ( Dae Ok Kim ),김금숙 ( Geum Soog Kim ),노형준 ( Hyung Jun Noh ),김계원 ( Gye Won Kim ),서우덕 ( Woo Duck Seo ),강희철 ( Hee Che 한국응용생명화학회(구 한국농화학회) 2012 Journal of Applied Biological Chemistry (J. Appl. Vol.55 No.4

The fruits of Acanthopanax sessiliflorus Seeman (Araliaceae) were extracted with 70% aqueous ethanol at room temperature. The concentrated extract was partitioned with ethyl acetate (EtOAc), n-butyl alcohol, and H2O, successively. From the EtOAc fraction, two compounds were isolated through the repeated silica gel, octadecyl silica gel, and Sephadex LH-20 column chromatographies. According to the results of physicochemical and spectroscopic data including NMR, mass spectrometry, and infrared spectroscopy, the chemical structures of the compounds were determined as 3,5-dihydroxycinnamic acid (1) and protocatechuic acid (2). Compound 1 was isolated from the fruits of A. sessiliflirus Seeman for the first time. And the compounds were evaluated for the radical scavenging the antioxidant capacity using 2,2`-azino-bis(3-ethylbenzthiazoline-6- sulfonic acid)diammonium salt, 1,1-diphenyl-2-picrylhydrazy, and oxygen radical absorbance capacity assay.

메모리 매핑 기반 MPI 노드 내 통신을 위한 라이브러리 수준 캐시 구현

김건우(Keon-Woo Kim),김준비(Joon-Bi Kim),김민범(Min-Bum Kim),김서우(Seo-Woo Kim),조중연(Joong-Yeon Cho),진현욱(Hyun-Wook Jin) 한국정보과학회 2019 한국정보과학회 학술발표논문집 Vol.2019 No.12

우황청심원의 고문헌기록 및 실험적 연구결과 분석을 통한 임상응용 확대의 필요성 고찰

오영택,오현묵,김서우,김원용,손창규,조정효,Oh, Young-Taeck,Oh, Hyeon-Muk,Kim, Seo-Woo,Kim, Won-Yong,Son, Chang-Gue,Cho, Jung-Hyo 대전대학교 한의학연구소 2017 혜화의학회지 Vol.26 No.1

Objectives: The aim of this study is (1) to investigate the historic changes and pharmacological efficacies of Woohwangchungsim-won and (2) to discuss the necessities for Woohwangchungsim-won's clinical applications. Methods: This study was performed through (1) investigating the ancient literature records related with Woohwangchungsim-won and analyzing Woohwangchungsim-won's composition, dosage and indications, (2) searching articles about Woohwangchungsim-won on 10 major Korean web and 3 major foreign web article search engines and analyzing Woohwangchungsim-won's pharmacological efficacies and indications. Results: Woohwangchungsim-wom has been used for cerebrovascular diseases such as stroke and palpitation. Also, there are some ancient literature records of Woohwangchungsim-won's clinical applications in neuropsychiatric disorders such as depression and bipolar disorder. In addition, there have been a number of experimental studies which demonstrate Woohwangchungsim-won's neuroprotective effect on cerebral cortex and hippocampus injury. So, it is possible to infer that Woohwangchungsim-won can be used for the treatments of neuropsychiatric disorders associated with neuronal cell death in cerebral cortex and hippocampus. But there have been no or less experimental studies which demonstrate the pharmacological efficacy of Woohwangchungsim-won on such disease. Conclusion: It is necessary that further experimental studies which demonstrate Woohwangchungsim-won's pharmacological efficacy on neuropsychiatric disorders should be done and Woohwangchungsim-won's clinical applications should be expanded on the basis of those related experimental results.

새싹보리 재배기간 중 수분스트레스 처리가 사포나린 함량에 미치는 영향

윤영은 ( Young-eun Yoon ),김송엽 ( Song Yoeb Kim ),최현지 ( Hyeonji Choe ),조주영 ( Ju Young Cho ),서우덕 ( Woo Duck Seo ),김영남 ( Young-nam Kim ),이용복 ( Yong Bok Lee ) 한국환경농학회 2021 한국환경농학회지 Vol.40 No.4

BACKGROUND: Barley sprouts contain a large number of secondary metabolites such as polyphenols, saponarin, and policosanols. The synthesis of such secondary metabolites occurs as a defense mechanism against external environmental stresses. In particular, it has been widely known that drought stress (DS) increases the content of flavonoids in plants. The objective of this study was to investigate the effects of drought stress treatment on the saponarin content in barley sprouts during the growing period. METHODS AND RESULTS: In this study, changes in saponarin content with different DS exposure periods and times were evaluated under the hydroponic system. For establishing different DS treatment periods, water supply was stopped for 1, 2, and 3 days, once leaf length was at 10 cm. To control different DS treatment times, water supply was stopped for 2 days, once leaf lengths were 5, 10, and 15 cm. As a result, the water potential of barley sprouts decreased from -0.8 MPa (before DS treatment) to -1.2, -2.4, and -3.2 MPa (after DS treatment), and reversely recovered to -0.8 MPa after re-irrigation. When 10 cm leaves were subjected to DS for 1 and 2 days, the saponarin content increased by 12 and 10%, respectively, while it increased by 19% when DS was applied to the 5 cm leaves. CONCLUSION(S): The results of this study suggest that drought stress at the early stage of growth (5 cm) is most helpful to increase the saponarin content of barley sprouts.

카메라를 이용한 2 차원 미소 진동 측정 연구

우정한(Jung-Han Woo),김서우(Seo-Woo Kim),이정권(Jeong-Guon Ih) 한국소음진동공학회 2016 한국소음진동공학회 학술대회논문집 Vol.2016 No.4