Effects of Intermittent Fasting on the Circulating Levels and Circadian Rhythms of Hormones

김보혜,주예나,김민선,최한경,Qingchun Tong,권오빈 대한내분비학회 2021 Endocrinology and metabolism Vol.36 No.4

Intermittent fasting has become an increasingly popular strategy in losing weight and associated reduction in obesity-related medicalcomplications. Overwhelming studies support metabolic improvements from intermittent fasting in blood glucose levels, cardiac andbrain function, and other health benefits, in addition to weight loss. However, concerns have also been raised on side effects including muscle loss, ketosis, and electrolyte imbalance. Of particular concern, the effect of intermittent fasting on hormonal circadianrhythms has received little attention. Given the known importance of circadian hormonal changes to normal physiology, potentialdetrimental effects by dysregulation of hormonal changes deserve careful discussions. In this review, we describe the changes in circadian rhythms of hormones caused by intermittent fasting. We covered major hormones commonly pathophysiologically involvedin clinical endocrinology, including insulin, thyroid hormones, and glucocorticoids. Given that intermittent fasting could alter boththe level and frequency of hormone secretion, decisions on practicing intermittent fasting should take more considerations on potential detrimental consequences versus beneficial effects pertaining to individual health conditions.

산복사나무 추출물의 궤양성 대장염증 완화 검증

김보혜,이용준,김희준,이미라,조계춘,라문진 강원대학교 농업생명과학연구원 2023 강원 농업생명환경연구 Vol.35 No.4

Prunus Davidiana, known as wild peach, has been used as a medicinal material against various inflammatory diseases since ancient times and belongs to the family Rosaceae. This study aimed to investigate the effect of wild peach extract (WPE) on inflammatory bowel diseases IBD at two different doses (10 and 100 mg/kg). Colitis was induced in mice (C57BL/6) by administering 2.0% Dextran sodium sulfate (DSS) in drinking water for 5 days. WPE treatment significantly improved the disease activity index, addressing symptoms such as body weight loss, bloody stool, stool consistency, and colon shortening. Furthermore, the increased levels of myeloperoxidase and pro-inflammatory cytokines (IL-6 and IL-22) with the development of colitis following DSS administration were significantly decreased by WPE. Histological analysis with Hematoxylin & Eosin staining of the colon tissue suggested that WPE significantly reduced colon damage. Additionally, pro-inflammatory cytokine (IL-8) and reactive oxygen species levels were reduced in HT-29 cells. Based on these results, we suggest that P. davidiana can ameliorate DSS-induced colitis in a mouse model. Furthermore, we anticipate that P. davidiana could serve as an effective therapeutic candidate for the treatment of inflammatory bowel diseases.

치매임상평가척도 박스총점(CDR-SB)에 따른 기억성 경도인지장애와 혈관성 경도인지장애의 MMSE, HDS-R 및MoCA 검사 반응 비교

김보혜,강연욱 대한치매학회 2014 Dementia and Neurocognitive Disorders Vol.13 No.4

Background: O’Bryant et al. (2008) argued that the CDR Sum of Boxes (CDR-SB) can be used to distinguish MCI from very early dementia in patients with CDR-GS of 0.5. They reported that the optimal CDR-SB cut-off score was 2.5. The present study was conducted to examine whether the subgroups classified with CDR-SB scores showed the corresponding group differences on the cognitive tests. Methods: The subjects were 45 amnestic multi-domain MCI (amMCI) and 53 vascular MCI (VaMCI) with CDR-GS of 0.5. Each patient group was classified into “mild (0.5-2.0)” and “severe (2.5-4.0)” subgroups based on the CDR-SB. As the result, 4 groups were formed such as mild amMCI (n=23), severe amMCI (n=22), mild VaMCI (n=29), and severe VaMCI (n=24). The subjects were given the MMSE, HDS-R, and MoCA. The MANCOVA was conducted separately for each test with a Bonferroni correction for multiple comparisons. Results: Severe groups of both amMCI and VaMCI showed significantly lower performances than two mild groups in MMSE, HDS-R, and MoCA. It was found that “serial 100-7” was a good item to discriminate between the mild and severe groups in both amMCI and VaMCI. Items measuring “orientation” or “naming” showed significant subgroup differences in the amMCI, whereas items related to “memory,” “visuospatial construction,” or “frontal/executive function” showed significant subgroup differences in the VaMCI. Conclusions: These results showed that there were significant differences in cognitive functions between the two subgroups with CDR-GS of 0.5. The results about the items discriminating between subgroups in amMCI and VaMCI were consistent with the previous findings about the progression of cognitive impairments in AD and VaD. Therefore, these results support the use of CDR-SB as a guideline for distinguishing MCI from early dementia.

대학생의 성별, 학년별, 전공별 스트레스에 관한 연구

김보혜,안윤정,정민영,차지영,최경원,김지현,김수지,이자형 이화여자대학교 간호과학대학 2004 이화간호학회지 Vol.- No.38

The purpose of this study was to investigate the level and effect of stress on physical and mental health. Based on that result, we offer the basic research to lessen students' stress and make a program to promote health condition effectively. Subjects were 526 university students. (male: 232(44.1%) female: 294(55.9%). composed of 7 majors: human studies&social science, management, Science&technology, education. law, medicine, entertainment. freshman 108(20.5%), sophomore 140(26.6%) junior 148(28.1%), senior 130(24.7%)) Subjects were filled with 127 questionnaires. T test and ANOVA were used to evaluate each stress levels. The .05 level of significance was the critical for this research. Also used the correlation to know the relation between stress levels and effects. The result of the study were as follows: 1. According to gender, male students got 2.37, female students got 2.46 on the stress level(p=.05). And also the belonging items showed female students got higher stresses than male students. 2. According to grade, the average score of stress level was 2.42. sophomores got the highest stress and freshmen got the lowest stress level. But that was not meaningful difference. freshman and sophomore got higher stress on studying. and except the freshman, all grades got severe stress on employment. 3. According to major, there were differences on university life and surroundings. On university life, the students who majored management got highest stress and the students who majored Education got the least stress. And on surroundings, the students who majored Law got highest stress. 4. According to gender, female students suffered from more stress effects than male students.(male 2.3725 female 2.4657 t:-1.968 p:.050) 5. According to grade, the average score of stress was 2.2285. sophomore got 2.3042 and junior got 2.2148 on stress effects. 6. According to major, The students who major in law got more stress effects caused by family than any other students. The students who major in entertainment and gymnastics got more stress effects than any other students in surroundings. 7. There were significant correlations between the stress level and effects(r.=847, p=.000).

엔진구동형 NiTi 파일의 근관성형능력 비교

김보혜,최경규,박상혁,최기운 大韓齒科保存學會 2010 Restorative Dentistry & Endodontics Vol.35 No.2

The purpose of this study was to compare the root canal shaping ability of 4 rotary NiTi instruments in simulated root canals. For the preparation of thirty two curved root canals, Mtwo instruments using “single length”technique, and Profile, ProTaper Universal, and K3 using crown-down technique (N = 8) were used. All canal samples were prepared by reaching an apical canal size of #30. Pre- and post-instrumentation digital images were recorded and an assessment of canal shape was determined using a computer image analysis program SigmaScan Pro (Systat Software Inc., San Jose, CA, USA). The changes of the dimension of inner walls of canals, (2) the changes of the dimension of outer walls of canals, and (3) the centering ratio were measured at 7 measuring points, and then data were statistically analyzed using one-way ANOVA and Duncan’s test. The results were as below; 1. The root canal shaping ability of Profile was significantly faster than that of other rotary NiTi instruments (p < 0.05). 2. The deformation and fracture of all instruments used for this study were not experienced. 3. In the degree of changes of the dimension of inner walls of canals, Profile demonstrated the lowest changes of the dimension of inner walls of canals except at the measuring points of the 1 and 2 mm (p < 0.05). However, the ProTaper Universal showed the highest changes of the dimension of inner walls of canals at all measuring points (p < 0.05). 4. In the degree of changes of the dimension of outer walls of canals, Mtwo demonstrated the lowest changse of the dimension of outer walls of canals except at the measuring point of the 1 mm (p < 0.05). However, Profile exhibited the highest changes of the dimension of outer walls of canals at the measuring points of 3 and 4 mm and ProTaper Universal and K3 showed the largest changes of the dimension of outer walls of canals at the measuring points of 1, 2, 6, and 7 mm (p < 0.05). 5. In degree of centering ratio, Profile demonstrated the least centering ratio comparing with the centering ratio shown by other NiTi instruments at the measuring points of 1, 4, 5, and 6 mm. Results suggest that in the coronal part of canal preparation, active cutting files such as ProTaper Universal may efficiently flare the canal orifice and form a better taper, and in the apical part of the canal, files which have a better centering ability such as Profile may maintain the original canal curvature and reduce the shaping time. 이 연구의 목적은 4가지 엔진구동형 NiTi 기구의 근관성형력을 비교하는 것이다. 32개의 투명레진모형을 사용하여 파일의 단면도가 S자 형태이고“single length”방법으로 근관성형을 시행하는 Mtwo와 crown-down 방법으로 근관성형을 하는 Profile, ProTaper Universal 및 K3로 근관성형을 하였다 (n = 8). 모든 근관은 한사람의 술자가 근단공 크기가 #30이 될때까지 성형하였다. 기구 사용 전과 후 근관 모양을 디지털 영상을 기록하였고, 컴퓨터 영상 분석 프로그램(Expression Scanner와 Adobe Photoshop CS)으로 평가하였다. 다음과 같은 결론을 얻었다. 근첨으로부터 1, 2, 3, 4, 5, 6, 7 mm 떨어진 부위의 내측과 외측으로 삭제된 레진 양을 측정하였고, 자료는 SPSS법으로 비교 분석하였다. 1. 근관형성 시간은 Mtwo가 84.50초로 가장 짧았으며, 총 작업시간은 K3가 269.37초로 가장 길었다(p < 0.05). 2. 기구의 변형과 파절은 모든 구동형 NiTi 파일에서 발생하지 않았다. 3. 근관 내측 폭경의 변화는 Profile이 1, 2 mm 부위를 제외한 다른 부위에서 가장 적었으며, ProTaper Universal은 모든 부위에서 가장 많았다(p < 0.05). 4. 근관 외측 폭경의 변화는 Mtwo이 1 mm 부위를 제외한 모든 부위에서 가장 적었으며, Profile은 3, 4 mm 부위에서 가장 많았다(p < 0.05). ProTaper Universal와 K3는 1, 2, 6, 7 mm 부위에서 가장 많았다(p < 0.05). 5. 근관중심변위율(centering ratio)은 Profile이 1, 4, 5, 6 mm 부위에서 가장 적었다(p < 0.05). 이상의 결과, 구동성 NiTi 파일을 이용한 만곡 근관의 근관형성은 치경부는 ProTaper와 같은 삭제력이 높은 파일을 사용하는 것이 근관 입구를 효과적으로 넓히고 더 나은 taper를 형성하며, 근단부는 Profile과 같은 근관 중심 변위가 적은 파일을 사용하는 것이 근관형성 시간을 단축하고 근단부의 근관 변형(transportation)을 줄일 수 있다고 판단된다.

디자인씽킹교육이 창업자기효능감, 창업의지와 문제해결능력간의 관계에 미치는 영향

김보혜,김창완 한국경영학회 2021 한국경영학회 통합학술발표논문집 Vol.2021 No.8

본 연구는 디자인씽킹교육이 창업자기효능감, 창업의지와 문제해결능력간의 관계에 미치는 영향을 검증하였다. 이를 위해 디자인씽킹 교과목을 수강한 대학생을 대상으로 수업 전, 수업 후에 2번에 걸쳐 설문을 진행하고, 회귀분석 및 T-test를 진행하였다. 연구결과로 창업자기효능감의 하위변수인 자신감, 자기조절효능감, 과업도전감은 문제해결능력에 유의한 영향을 미치는 것으로 나타났다. 또한 자신감, 자기조절효능감, 과업도전감이 창업의지에 미치는 영향을 문제해결능력이 완전 매개하는 것으로 확인되었다. 본 연구결과는 실무적으로는 창업교육이 실제 창업으로 전환되기 위해서는 개인적인 특성인 창업자기효능감과 문제해결능력을 강화할 수 있는 교과목의 편성이 요구되며 창업교육 실태조사의 방식을 문제해결능력, 창업자기효능감 등의 역량 중심으로 전환하고 대학에서 콘텐츠 개발을 지원할 수 있는 지원정책이 필요함을 제시한다.

Dopamine depletion alters neuroplasticity-related signaling in the rat hippocampus

김보혜,김중선,윤부현,문창종 한국통합생물학회 2023 Animal cells and systems Vol.27 No.1

Dopamine (DA) plays a significant role in regulating hippocampal function, particularly inmodulating synaptic plasticity. Despite this, a comprehensive understanding of the molecularmechanisms involved in neuroplasticity-related signaling influenced by DA remains incomplete. This study aimed to elucidate the changes in the expression of key molecules related tohippocampal neuroplasticity following DA depletion in rats. To induce DA depletion, unilateralstriatal infusions of 6-hydroxydopamine (6-OHDA) were administered to adult Sprague-Dawleyrats. The subsequent loss of nigrostriatal DAergic signaling in these 6-OHDA-lesioned rats wasconfirmed using an apomorphine-induced rotation test at 4 weeks post-infusion and byassessing the expression levels of tyrosine hydroxylase (TH) through immunohistochemistry andwestern blotting at 7 weeks post-infusion. A decrease in DAergic signaling, evidenced byreduced TH-positive immunoreactivity, was also noted in the ipsilateral hippocampus of thelesioned rats. Interestingly, 6-OHDA infusion led to increased phosphorylation of pivotalhippocampal plasticity-related proteins, including extracellular signal-regulated kinase (ERK),protein kinase B (Akt), glycogen synthase kinase 3β (GSK3β), and cAMP response elementbindingprotein (CREB), in the ipsilateral hippocampus 7 weeks following the infusion. Toextend these findings, in vitro experiments were conducted on primary hippocampal neuronsexposed to DA and/or the active D1/D2 DA receptor antagonist, flupentixol (Flux). DA inhibitedthe constitutive phosphorylation of ERK, Akt, GSK3, and CREB, while Flux restored thesephosphorylation levels. Taken together, these findings indicate that DA depletion triggers anincrease in plasticity-related signaling in the hippocampus, suggesting a possible compensatorymechanism that promotes activity-independent neuroplasticity following DA depletion.

연고기제의 침투력 시험

김보혜,조순자,조화옥,이경구,한형주 이화여자대학교 약학회 1966 梨花藥學會誌 Vol.- No.7

The epidermis is wholly covered with fat film and can not allow to penetrate some substances(medicaments) through the skin. Then hydrophillic substances are difficult to penetute through the healthy skin because of epidermis is hypophillic property but when the skin should be wounded by circumstances, secretion squeezes out of the inner tissue and spreads on the surface of the skin to form hydrophillic property. We planned to study for the penetration of the ointment bases with agar-culture instead of wounded skin, because the agar-culture is as similar as the wounded skin in every condition. The Ointment bases used for study are classified as following 4 groups. 1. Oleaginous bases: vaselin, lanolin, lanolin+vaselin. 2. Absorption bases: cold cream. 3. Emulsion bases: vanishing cream hydrophillic ointment. 4. Water soluble bases: carbowax. Consequently, we got such experimental results that watersoluble bases are the best of all in penetration.

백파의 삼종선에 관한 초의의 비판에 대한 고찰

김보혜 한국동서철학회 2018 동서철학연구 Vol.0 No.90

The dispute on the theory of Seon is the dispute over the doctrine of Seon between Baekpa-Geungseon and Choui-Uisun, its point is made up of the dispute on three kinds of Seon and two kinds of Seon, and the dispute has been continued by the two Seon teachers' disciples. While there was 'a dispute on the same and difference of human nature and material nature' in Korean Confucianism, there was 'a dispute on three kinds of Seon and two kinds of Seon'. Although there is a lot of dispute on the 'three kinds of Seon and two kinds of Seon', this thesis will first introduces the contents and the background of its appearance, and examine what is the issue and gist of Choui's criticism of three kinds of Seon. Through this study, I would like to discuss whether Choui's criticism of three kinds of Seon is valid. Baekpa advocated the three kinds of Seon in Seonmunsugyong(禪文手鏡), whose name comes from the division of the levels of Seon into Patriarch Seon(祖師禪), Tathāgata Seon(如來禪), and Principle Seon(義理禪). On the contrary, Choui disputes Baekpa's Seonmunsugyong in his Seonmunsabyeonmaneo(禪門四辨漫語), which has a form that first introduces Baekpa's theory and then criticizes it. It seems that Baekpa's attempt to allocate all Seon analects to one of the three kinds of Seon intentionally goes too far and undermines the original spirit of Seon according to Choui's criticism. However, it can be said that Baekpa's three kinds of Seon is evidently valuable in that his theory presented the standard to judge the depth and shallowness of Seon that is meant to realize the original face beyond language and logic. 선문논쟁(禪門論爭)이란 조선 후기 고승이었던 백파 긍선(白坡亘璇)과 초의 의순(草衣意恂)사이에서 촉발된 선(禪)에 대한 교리논쟁으로 삼종선(三種禪)과 이종선(二種禪)에 대한 논쟁이 그 핵심을 이루며, 이후 무려 100년이 넘는 시간동안 두 선사의 제자들을 통해서 이어져 나갔다. 18-19세기 한국유학에 ‘인물성동이(人物性同異)논쟁’이 있었다면, 불교계에는 ‘삼종선-이종선 논쟁’이 있었던 것이다. ‘삼종선-이종선’에 관해서는 많은 논쟁거리가 있지만, 본 논문에서는 먼저 백파의 삼종선의 내용과 출현 배경에 대하여 소개한 후, 초의가 삼종선을 비판한 쟁점과 요지가 무엇인가를 살펴 볼 것이다. 이를 통해 ‘초의의 삼종선 비판은 과연 타당한 것인가?’하는 문제에 대하여 논하고자 한다. 백파는 『선문수경(禪文手鏡)』을 통하여, 삼종선을 주창하는데, 삼종선이란 선을 그 경지에 따라 ‘조사선(祖師禪), 여래선(如來禪), 의리선(義理禪)’의 세 종류로 나누어 보는 데에서 붙여진 이름이다. 반면, 초의는 『선문사변만어(禪門四辨漫語)』를 통하여 백파의 『선문수경』을 반박하고 있는데, 먼저 백파의 설을 소개한 후 자신의 비판을 가하는 형식을 띄고 있다. 모든 선어록의 말들을 인위적으로 삼종선의 하나에 배대하려한 백파의 시도는 지나친 점이 있으며, 초의의 지적대로 선의 본래정신을 훼손한 면이 있다고 보여 진다. 그러나 언어와 논리를 떠나 본래면목을 곧장 깨닫고자 하는 선의 특성상 그 깊고 얕음을 판단할 수 있는 기준을 제시했다는 점에서 백파의 삼종선은 분명 가치가 있다고 할 것이다.

Current Status and Associated Factors of Emotional Distress Due to COVID-19 Among People with Physical Disabilities Living in the Community: Secondary Data Analysis using the 2020 National Survey of Disabled Persons

김보혜,윤주영 한국지역사회간호학회 2024 지역사회간호학회지 Vol.35 No.1

Purpose: This study aimed to assess the current situation and factors associated with emotional distress due to COVID-19 among people with physical disabilities living in the community, using a Social-Ecological Model.Methods: A secondary data analysis was performed using data from the 2020 National Survey of Disabled Persons. The study participants were 1,654 participants aged 19 years or older with physical disabilities. For data analysis, complex sample logistic regression was conducted to identify factors associated with emotional distress due to COVID-19 in people with physical disabilities.Results: The results showed that 55.7% of people with physical disabilities living in the community experienced emotional distress due to COVID-19. This emotional distress was influenced by various factors, including intrapersonal factors (activities of daily living, difficulty in getting information about COVID-19, difficulty in obtaining and using equipment related to COVID-19), interpersonal factors (satisfaction with the number of friends), and community/policy factor (support related to COVID-19 from national and local governments).Conclusion: It is recommended to develop tailored interventions to prevent mental health issues among people with physical disabilities by considering the factors related to their emotional distress in the event of future infectious disease disasters.