고추좀잠자리 (Sympetrum depressiusculum)로부터 분리한 리그닌 분해균주, Serratia marcescens HY-5의 특성

김기덕,박두상,신동하,한보나,오현우,윤영남,박호용,Kim, Ki-Duck,Park, Doo-Sang,Shin, Dong-Ha,Han, Bo-Na,Oh, Hyun-Woo,Youn, Young-Nam,Park, Ho-Yong 한국응용곤충학회 2006 한국응용곤충학회지 Vol.45 No.3

A ligniolytic bacterial strain was isolated from the digestive tract of a red dragonfly, Sympetrum dopressiusculum. It was identified as a Serratia marcescens HY-5 by 16S rDNA sequence analysis and physiological and biochemical analysis. The isolated strain showed proportional increase of ligninolytic activity to the cell growth in the culture media which include lignin compounds. It showed about 25-45% decomposition of lignin compound by 48 hr incubation especially, showed effective decomposition of monomer lignin compounds, vanillin and guaiacol, and a dimer, dealkaline lignin. PCR amplification of 16S rDNA followed by denaturing gradient gel electrophoresis analysis showed high density of S. marcescens HY-5 in the gut of the S. depressiusculum at both gut samples which collected at different site. 고추좀잠자리의 장으로부터 리그닌 분해활성을 보이는 미생물을 분리하였으며 16s rDNA 서열분석 및 생리 생화학적 동정에 의해 Serratia marcescens에 속하는 새로운 균주로 밝혀졌다. 분리된 균주는 리그닌 화합물을 포함하는 배지에서 배양하였을 때 cell growth의 증가에 따라 리그닌 화합물에 대한 분해능이 증가하였으며 48시간의 배양에 의해 20-45%의 분해능을 나타내었고, 특히 monomer 화합물인 vanillin 및 guaiacol과 dimer 화합물인 dealkaline 리그닌에 대한 분해능이 높았다. 분리된 균주 S. marcescens HY-5는 PCR에 의한 16S rDNA의 증폭과 denaturing gradient gel electrophoresis에 의한 장내 세균의 분포를 조사하였을 때 높은 밀도의 분포를 나타내었으며 서로 다른 지역에서 채집된 고추좀잠자리에서 공통적으로 발견되는 특징을 보여주었다.

조선후기(朝鮮後期) 충청도(忠淸道) 전의현(全義縣) 관아건축(官衙建築)의 배치구성(配置構成)에 관한 연구(硏究)

김기덕,이재헌,Kim, Ki-Deok,Lee, Jae-Heon 한국건축역사학회 2001 건축역사연구 Vol.10 No.4

This study is to analyze site layout traditional government office building focused on Chonui-hyon(全義縣) in Chungchong province with Chungchong-do regional maps(忠淸道地方地圖) and Eupjis(邑誌, topography) being compiled in the late Chosun dynasty. The conclusion of analysis can be summarized as follows; 1. Chonui-hyon(全義縣) in Chosun dynasty is gone with a planning principle of the capital city(Han-Seong, 漢城) with disposing Sa-Ji-Dan(社稷壇) and Gaek-Sa(客舍), which are placed to the left and right of Eupchi(邑治), and it is followed in the wake of the spatial structure of Chosun dynasty palace with disposing Dong-Heon(東軒) in front, Nae-A(內衙) at the back of it, or from side to side, also by the Dong-Heon of the center, with organizing each facility around it. 2. Dong-Heon and Gaek-Sa of Chonui-hyon is passed through three step gate, Mun-Ru(門樓), Woi-Sammun(外三門), Nae-Sammun(內三門), from Hong-Salmon(紅門), and are shown hierarchy by haying been placed at the end of approach axis. 3. Dong-Heon can be disposed by Feng-Shui(風水) which have influence on the southeast direction with Jin-San(鎭山) and An-Dae(案對), on the other hand, Gaek-Sa can be disposed by symbolic of the authority of a king. 4. Site layout by function of provincial government office is provided into four ferrite, Dong-Heon, Gaek-Sa, Nae-A, practical business territory, and eve territory is organized by official institution on a social position to radiation, by the compositive axis to be made Woi-Sammun Nae-Sammun Dong-Heon Nae-A 5. Approach process of Chnui-hyon government office is three door system similar to Kam-Young(監營) in a different way two door system in most provincial government office, also approach axis is formed by bending.

초소형 위성의 랑데부/도킹 기술 검증을 위한 시나리오 설계

김기덕,김해동,조동현,Kim, Kiduck,Kim, Hae-Dong,Cho, Dong-Hyun 한국우주과학회 2022 우주기술과 응용 Vol.2 No.1

This paper illustrates the trajectory design of drift distance recovery after initial launch and proximity operation when verifying rendezvous/docking technology using nanosatellites. The rendezvous/docking is a technology that is the basis of on-orbit servicing technology and is a preemptive process essential for approaching a target object. In particular, since it is difficult to verify in space, nanosatellites have recently been used to reduce the risk and cost of the development stage. Therefore, this paper not only introduces the configuration and specifications of thrusters for nanosatellites but also designs relative trajectories that can take into account the thrust limitations which come from the small size and low power of nanosatellites. In addition, we intend to be helpful in later designing scenarios according to the improvement of available thruster performance through comparison of trajectories and thrust usage with cases without thrust limitations.

Calonectria ilicicola 의 병원성과 배양적 특성간의 상호관계

김기덕,( K . D . Kim,J . S . Russin,J . P . Snow,R . A . Valverde ) 한국균학회 1998 韓國菌學會誌 Vol.26 No.3

Amino Acid and Phenolic Contents in lilfected Leaves of Rice in Relation to Adult - Plant Resistance to Leaf Blast

김기덕,황병국,Kim Ki Deok,Hwang Byung Kook The Korean Society of Plant Pathology 1987 Plant Pathology Journal Vol.3 No.1

도열병에 대해 감수성을 보이는 낙동과 성체식물저항성을 보이는 도봉의 건전엽과 도열병 감염엽내의 에탄올가용성 아미노산과 페놀화합물을 양적으로 분석하였다. 접종 3일후에는 가용성 아미노산과 페놀화합물의 수준은 건전엽과 비슷하였다. 도열병 병반이 접종 5일 후에 감염엽에 나타났을 때 가용성 아미노산과 페놀화합물은 5엽기와 3엽기에서 증가하기 시작하였고, 접종 7일 후에서는 이들 화합물의 수준은 5엽기와 8엽기에서 건전엽보다 약 $1.5\~3$배 더 많았다. 도열병 감염동안 비록 도봉이 도열병에 대해 낙동보다 덜 감염되었지만 5엽기와 8엽기의 건전엽과 감염엽에서 성체식물저항성 품종 도봉의 가용성 아미노산 및 페놀화합물 함량은 감수성 품종 낙농의 함량보다 높았다. 벼품종 도봉이 도열병에 감염동안 아미노산과 페놀화합물 함량이 현저하게 증가하여 성체식물저항성이 발현하는데 중요한 역할을 할지 모른다. Ethanol-soluble amino acids and phenolics in healthy and blast-infected leaves of the susceptible rice cultivar Nakdong and adult-plant-resistant cultivar Dobong were quantitatively analyzed, At the 3 days after inoculation, the levels of soluble amino acids and phenolics in the infected fifth and eighth leaves of the two cultivars ere similar to those of comparable healthy controls. As blast lesions appeared on the leaves at the 5 days after inoculation, the soluble amino acids and phenolics began to increase. At the 7 days after inoculation, the levels of amino acids and phenolics were about 1.5-3 times more than those in healthy controls at the five-and eight leaf stages. The adult-plant-resistant cultivar Dobong showed higher amounts of soluble amino acids and phenolics in both healthy and infected fifth and eighth leaves than did the susceptible cultivar Nakdong, although Dobong was less infected by Pyricularia oryzae than Nakdong, The pronounced increases in amino acids and phenolics in rice leaves of the cultivar Dobong during the blast infection may play an important role in the expression of adult-plant resistance to blast.

하악골 매식 부위 계측을 위한 나선형 단층촬영술의 신뢰도

김기덕,박창서,Kim Kee-Deog,Park Chang-Seo 대한영상치의학회 1997 Imaging Science in Dentistry Vol.27 No.2

The purpose of this study was to evaluate the accuracy and usefulness of spiral tomography through the comparison and analysis of SCANORA cross-sectional tomographs and DentaScan computed tomographic images of dry mandibles taken by a SCANORA spiral tomographic machine and a computed tomographic machine. Thirty-one dry mandibles with full or partial edentulous areas were used. To evaluate the possible effect of location in the edentulous area, it was divided into 4 regions of Me (region of mental foramen), MI (the midportion between Me and M2), M2 (the midportion between mental foramen and mandibular foramen) and S (the midportion of the mandibular symphysis). A ZPC column (sized 4 mm x 5 mm) was seated on the edentulous regions of Me, MI, M2 and S using the acrylic stent. Then SCANORA spiral tomography and computed tomography were taken on the edentulous regions which contained the ZPC column. The ZPC columns and cross-sectional images of the mandible were measured in the radiographs by three observers and the differences between the two imaging modalities were analysed. The results were as follows: 1. In comparing the actual measurements of the ZPC column and measurements in the radiographs, the mean error of the DentaScan computed tomography was 0.07 mm in vertical direction and -0.06 mm in horiwntal direction, while the mean error of the SCANORA spiral tomography was 0.06 mm in vertical direction and -0.12 mm in horizontal direction. There was a significant difference between the two radiographic techniques in the horizontal measurement of the ZPC column of the symphysis region (p<0.05). But there was no significant difference in the measurements of other regions (p>0.05). 2. In measurements of the distance from the alveolar crest to the inferior border of the mandible (H), and of the distance from the alveolar crest to the superior border of the mandibular canal (Y), there was no significant difference between the two radiographic techniques (p>0.05). 3. In measurements of the distance from the lingual border of the mandible to the buccal border of the mandible (W), and of the distance from the lingual border of the mandible to the lingual border of the mandibular canal (X), there was a significant difference between the two radiographic techniques in measurements of the midportion between the mental foramen and the mandibular foramen (M2) (p<0.05). But there were no significant differences in measurements of the other regions of symphysis (S), mental foramen (Me), the first one-fourth portion between the mental foramen and the mandibular foramen (M1) (p>0.05). 4. Considering the mean range of measurements between observers, the measurements of SCANORA spiral tomography showed higher value than those of DentaScan computed tomography, except in measurements of symphysis (S). 5. On the detectability of the mandibular canal, there was no significant difference between the two radiographic techniques (p>0.05). In conclusion, SCANORA spiral tomography demonstrated a higher interobserver variance than that of DentaScan computed tomography for implant site measurements in the posterior edentulous area of the mandible. These differences were mainly the result of difficulty in the detection of the border of the mandible in SCANORA spiral tomography. But considering the cost and the radiation exposure, SCANORA spiral tomography can be said to be a relatively good radiographic technique for implant site measurement.

조선후기(朝鮮後期) 충청도(忠淸道) 지방(地方) 읍치(邑治)의 공간구조(空間構造)에 관한 연구(硏究)

김기덕,이재헌,Kim, Ki-Deok,Lee, Jae-Heon 한국건축역사학회 2003 건축역사연구 Vol.12 No.1

This study is to analyze spatial structure of Eupchi(邑治) on Chungchong province with Chungchong-do regional maps(忠淸道地方地圖) and Eupjis(邑誌, topography) being compiled in the late Chosun dynasty. According to the analysis of it in this study, we make conclusions as follows; 1. Gunhyeon(郡縣) which had been Eupseong(邑城) on Chungchong-do in Chosun dynasty was 13 provinces, and the construction of Eupseong was the coast Eupseong built for the purpose of defense and military Eupseong built at Byeongyeong(兵營) and Geojin(巨鎭). And a measure used In the construction of a castle was Pobaekcheok(布帛尺) used to survey a frontier defence in Chosun dynasty, also Jucheok(周尺) or Yeongjocheok(營造尺) could be assumed to be wide use at that time. 2. Eupchi of Chungchong-do Gunhyeon was almost disposed to the south direction, also had been Jinsan(鎭山) safeguarding it. With relation to Jwahyang(坐向) and Jinsan, its Jinsan and Myeongdang-cheon(明堂川) match each other in location of Eupchi, as it get Jinsan sat, and take main river in front of it. And provincial government office to be the center of a Eupchi is organized into Gaeksa(客舍), Dongheon(東軒), Naea(內衙), Hyangcheong(鄕廳), and practical business facilities, Jakcheong(作廳) or Jangcheong etc., the others is composed for the use of support of those. 3. In most Eupchi in Chungchong province, the layout Sajikdan(社稷壇) and Yeodan(礪壇) was gone with a principle as they were disposed in the west and the north with Eupchi. Seonghwangdan(城隍壇) and Munmyo(文廟) was built in defiance of a principle, as a condition of province. Jangsi(場市) of Eupchi was opened in front of government office or Gaeksa, and the Gunhyeon which had Eupseong was established in the inside and outside of Eupseong.

Development of Safety Sensor for Vehicle-Type Forest Machine in Forest Road

김기덕,Hyun-Seung Lee,Gyun-Hyung Kim,신범수 강원대학교 산림과학연구소 2023 Journal of Forest Science Vol.39 No.4

A sensor system has been developed that uses an ultrasonic sensor to detect the downhill slope on the side of a forest road and prevents a vehicle-type forest machine from rolling down a mountainside. A specular reflection of ultrasonic wave might cause severe issues in measuring distances to targets. By investigating the installation angle of the sensor to minimize the negative effects of specular reflection, the installation angle of lateral monitoring ultrasonic sensor could be determined based on the width of road shoulder. Obstacles such as small rocks or piece of log in a forest road may cause the forest machine to be overturned while the machine riding over due to excessive its posture change. It was determined that the laser sensor could be a part of a sensor system capable of specifying the location and size of small obstacles. Not only this sensor system including ultrasonic and laser sensors can issue a warning of dangerous sections to drivers in forest forwarders currently in use, but also it can be used as a driving safety sensor in autonomous forest machine or remote-control forest machine in the future.

계분퇴비와 천연무기질 자재를 활용한 고랭지배추 비가림 유기재배 시 시용질소의 이용률과 배추의 생육

김기덕,권영석,유동림,이종남,서종택,Kim, Ki-Deog,Kwon, Yeong-Seok,Yoo, Dong-Lim,Lee, Jong-Nam,Seo, Jong-Taek 한국유기농업학회 2013 韓國有機農業學會誌 Vol.21 No.1

This study was carried out to evaluate nitrogen availability of applied fertilizer and to investigate yield and growth of Korean kimchi cabbage as affected by amount of fertilizer and soil fertility in organic farming applied various fertilizers in rainshelter. The head weight of Korean kimchi cabbage cultured in infertile soil (sand loam) with no amendments was very low. and that in fertile soil (clay loam) was higher than in infertile soil (sand loam). The head weight of Korean kimchi cabbage as affected by amount of fertilizer was more variable in infertile soil (sand loam) than in fertile soil (clay loam). Nitrogen availability of applied fertilizer by Korean kimchi cabbage was lower in fertile soil (clay loam) than in infertile soil (sand loam) and the lower that was, the more fertilizer applied. By application of poultry manure compost 20Mg ha-1 and natural mineral materials such as guano, phosphate rock, and potassium magnesium rock equal to amount of fertilizer recommended in conventional farming, the yield of Korean kimchi cabbage in infertile soil (sand loam) with 1% organic matter came up to 90% of the yield in fertile soil (clay loam) with 6% organic matter. Therefore natural mineral materials such as guano for N source, phosphate rock for P source, and potassium magnesium rock for K source may be able to use as environmental-friendly fertilizers in organic Korean kimchi cabbage production in highland. 비가림하우스에서 고랭지배추 유기재배 시 시용량 및 토양비옥도에 따른 시용질소의 이용률과 배추의 수량성을 검토하였다. 무비구에서의 구중은 사양토 토양에서 현저히 낮았으며, 비옥도가 낮은 사양토에 비해 비옥도가 높은 식양토에서 높았다. 시비량에 따른 구중의 변화는 비옥도가 높은 식양토에 비해 비옥도가 낮은 사양토에서 더 크게 나타났다. 질소이용률은 토양의 비옥도가 높고, 질소시비량이 많을수록 낮았다. 질소함량 1%의 사양토에서도 계분퇴비 2톤/10a과 표준시비량 수준의 천연무기질자재의 시용으로 질소함량 6%의 식양토의 약 90% 수준의 수량을 나타냈다. 질소, 인산, 및 칼리질 천연광물자원인 구아노, 인광석 및 황산가리고토 등은 친환경 자재로서 균형적 시비를 꾀하는 유기재배에서 널리 활용될 수 있을 것으로 보인다.

악관절원판 천공의 방사선학적 연구

김기덕,박창서,Kim Kee-Deog,Park Chang-Seo 대한영상치의학회 1990 Imaging Science in Dentistry Vol.20 No.2

Thirty-nine patients (forty-four joints) who had been diagnosed as having meniscus perforation of the temporomandibular joint by inferior joint space arthrography and had been treated by surgical procedures were evaluated retrospectively. Information of clinical findings, arthrotomographic findings and surgical findings was collected on a standardized form and evaluated. The results were as follows: 1. On the 34 patients of 38 joints which were surgically confirmed perforation of meniscus or its attachments of the temporomandibular joint, there were 29 females and 5 males (5.8:1). The average age was 36 years (range 17 to 70). 2. The common clinical findings of group that had meniscus displacement without reduction and with perforation were pain on the affected joint and limitation of mouth opening. In the group showing meniscus displacement with reduction and with perforation the common clinical findings were pain and clicking on the affected joint. 3. 32 joints (84.2%) were arthrotomographically anterior meniscus displacement without reduction and with perforation, 6 joints (15.8%) showed anterior meniscus displacement with reduction and with perforation. 4. Joints categorized arthrotomographically as having meniscus displacement without reduction and with perforation were less likely to have full translation of the condyle in comparison with the normal or meniscus displacement with reduction and with perforation groups. (p<0.05) 5.The arthrographic findings of 44 joints having meniscus perforation were compared with surgical findings, there were 6 false positive findings of meniscus perforation, the reliability of arthrographic findings of meniscus perforation was a 86.4% correlation with surgical findings. 6. On the site of perforations of 38 joints which were surgically confirmed perforation of meniscus or its attachments, twenty-three of perforations (60.5%) were in location at the junction of the meniscus and posterior attachment, forteen (36.9%) were located at the posterior attachment and one (2.6%) was at the meniscus itself.