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옵티컬 플로우 기반 장면 모델링을 통한 교통 영상 내의 이상 상황 인식 시스템
권언혜 ( Eonhye Kwon ),노승종 ( Seungjong Noh ),전문구 ( Moongu Jeon ) 한국정보처리학회 2012 한국정보처리학회 학술대회논문집 Vol.19 No.2
최근 카메라 센서 및 알고리즘의 발달로 엔터테인먼트 목적의 영상 시스템을 비롯한 공정 기술, 교육 및 의료 등 다양한 목적의 영상 시스템이 개발 되고 있다. 또한 범죄 예방, 사고 상황 인식을 위한 감시 영상 시스템의 연구도 활발히 진행되고 있다. 본 논문에서는 이상 상황 인식을 위한 지능형 교통 시스템에 대해 제안하고자 한다. 제안하는 시스템은 크게 학습 과정과 이상 상황 인식 과정으로 나누어진다. 학습 과정에서는 CCTV와 같은 정적인 카메라에서 촬영된 도로 교통 영상에서 이동 객체의 특징을 추출하고 이를 추적하여 특징 벡터를 구성한다. 구성된 특징 벡터들은 클러스터링 기법을 통해 장면을 모델링하는데 이용되며 최종적으로 이 모델을 이용해 실시간으로 도로 교통 영상에서 이상 상황을 인식할 수 있게 된다. 실험을 통한 성능 평가를 통해 시스템의 우수함을 확인 하였다.
중합효소연쇄반응 기법을 이용한 돼지 증식성 장염 진단 기법의 확립
김대영,권언혜,송민동,양시용 建國大學校 自然科學硏究所 2002 建國自然科學硏究誌 Vol.13 No.1
This study was carried out to establish a efficient diagnosis method of pocine proliferative enteropathy (PPE) contributing major economic loss by inducing weight loss, poor growth and/or sudden death through out the growth period(6~22 wk). Therefore, in this study we established the PCR (polymerase chain reaction) which was a fast, specific and sensitive method for identification of Lawsonia intracellularis (L. intracellularis). We designed and synthesized primer on the 16S rDNA and p78 gene encoding L. intracellularis. Specificity of the method was confirmed by comparison of the PCR results using other enteric bacteria and the study has shown that PCR method was sensitive to detect lng of genomic DNA as a template. Also, the PCR method was applicable to the naturally affected pigs with PPE. Based on the results from this study, the PCR method could be used as a fast and specific dignistic tool for PPE.
Pulsed Electromagnetic Field Stimulates Cellular Proliferation in Human Intervertebral Disc Cells
이환모,권언혜,김향,김호중,김보람,박진오,문은수,문성환 연세대학교의과대학 2010 Yonsei medical journal Vol.51 No.6
Purpose: The purpose of this study is to investigate the mechanism of cellular proliferation of electromagnetic field (EMF) on human intervertebral disc (IVD) cells. Materials and Methods: Human IVD cells were cultured three-dimensionally in alginate beads. EMF was exposed to IVD cells with 650 Ϊ, 1.8 millitesla magnetic flux density, 60 Hz sinusoidal wave. Cultures were divided into a control and EMF group. Cytotoxicity, DNA synthesis and proteoglycan synthesis were measured by MTT assay, [3H]-thymidine, and [35S]-sulfate incorporation. To detect phenotypical expression, reverse transcription-polymerase chain reactions (RT-PCR) were performed for aggrecan, collagen type I, and type II mRNA expression. To assess action mechanism of EMF, IVD cells were exposed to EMF with NG-Monomethyl-L-arginine (NMMA) and acetylsalicylic acid (ASA). Results: There was no cytotoxicity in IVD cells with the EMF group in MTT assay. Cellular proliferation was observed in the EMF group (p < 0.05). There was no difference in newly synthesized proteoglycan normalized by DNA synthesis between the EMF group and the control. Cultures with EMF showed no significant change in the expression of aggrecan, type I, and type II collagen mRNA compared to the control group. Cultures with NMMA (blocker of nitric oxide) or ASA (blocker of prostaglandin E2) exposed to EMF demonstrated decreased DNA synthesis compared to control cultures without NMMA or ASA (p < 0.05). Conclusion: EMF stimulated DNA synthesis in human IVD cells while no significant effect on proteoglycan synthesis and chondrogenic phenotype expressions. DNA synthesis was partially mediated by nitric oxide and prostaglandin E2. EMF can be utilized to stimulate proliferation of IVD cells, which may provide efficient cell amplification in cell therapy to degenerative disc disease.
아텔로콜라겐 지지체, 유전자 치료, 배양된 수핵세포를 이용한 조직 공학적 추간판의 재생
김학선,이광일,김향,권언혜,남미란,장주웅,조인제,김보람,이환모,문성환 대한척추외과학회 2010 대한척추외과학회지 Vol.17 No.2
Study Design: This is an in-vitro experiment using rabbit intervertebral disc (IVD) cells and growth factors. Objectives: We wanted to determine the effect of types I,and II atelocollagen and growth factor gene therapy for matrix regeneration of rabbit IVD cells. Summary of the Literature Review: Adenovirus-medicated growth factor gene therapy is efficient for matrix regeneration of the IVD. Atellocollagen has provided a favorable environment for matrix synthesis. However, a combined approach using gene and cell therapy in an atelocollagen scaffold has not yet been attempted. Materials and Methods: Rabbit IVD cells were transduced with Ad/TGF-β1 and Ad/BMP-2. The cells were then implanted to the atelocollagen scaffold. The [methyl-3H]thymidine incorporation for DNA synthesis and the [35S]sulfur incorporation for proteoglycan synthesis were measured. RT-PCR was performed for assessing the aggrecan, collagen type I, collagen type II and osteocalcin mRNA expressions. Results: The rabbit IVD cells with Ad/TGF-β1 and that were cultured in type I atelocollagen showed a 130% increase in new proteoglycan synthesis, while the rabbit IVD cells with Ad/TGF-β1 and that were cultured in type II atelocollagen showed a 180% increase in new proteoglycan synthesis (p<0.05). The rabbit IVD cells with Ad/BMP-2 and that were cultured in type I atelocollagen showed a 70% increase in new proteoglycan synthesis, while the rabbit IVD cells with Ad/BMP-2 and that were cultured in type II atelocollagen showed a 95% increase (p<0.05). Rabbit IVD cells with Ad/TGF-β1 and Ad/BMP-2 and that were cultured in type I and II atelocollagen demonstrated increased collagen type I and II mRNA expressions without an osteocalcin mRNA expression (p<0.05). Conclusion: Cell and gene therapy in an atelocollagen scaffold provided a efficient mechanism for chondrogenic matrix regeneration of rabbit IVD cells. 연구 계획: 토끼의 추간판 세포와 제5형 아데노바이러스(Ad) transforming growth factor beta-1(TGF-β1), 아데노바이러스 bone morphogenetic protein-2 (BMP-2), 아텔로콜라젠을 이용한 실험적 연구목적: 제1, 2형 아텔로콜라젠과 성장 인자 유전자 치료의 토끼 추간판 세포의 기질 생성 반응을 알아보았다. 선행문헌의 요약: 아데노바이러스를 이용한 성장 인자 유전자 치료는 추간판 세포의 기질 재생에 효과적이다. 아텔로콜라젠은 추간판 세포의 기질 합성에 유리한 환경을 제공한다. 하지만 아텔로콜라젠 지지체에 유전자 치료를 병행하는 방법은 시도된 바가 없었다. 대상 및 방법: 토끼의 추간판 세포를 배양하였다. Ad/TGF-β1, Ad/BMP-2 아텔로콜라젠을 생산하였다. 추간판 세포를 Ad/TGF-β1, Ad/BMP-2에 감염시키고 이 세포를 아텔로콜라젠 지지체에 배양하였다. DNA생성은 [methyl-3H] Thymidine을 이용하였고 새로운 당단백 생성은 [35S]Sulfur를 이용하였다. 또한 전사 수준에서의 추간판세포 관련 유전자의 변화를 확인하고자 RT-PCR을 통한 aggrecan, 제 1, 2형 교원질, osteocalcin mRNA발현을 조사하였다. 결과: 제1형 아텔로콜라젠에 배양되고 Ad/TGF-β1로 유전자 전달된 추간판 세포군은 대조군에 비해 130% 신생 당단벡 생성증가가 있었고 제 2형 아텔로콜라젠에 배양되고 Ad/TGF-β1로 유전자 전달된 세포군은 180%의 신생 당단백 생성 증가가 있었다. (p<0.05) 제 1형 아텔로콜라젠에 배양되고 Ad/BMP-2로 유전자 전달된 추간판 세포군은 대조군에 비해 70% 신생 당단벡 생성증가가 있었고 제2형 아텔로콜라젠에 배양되고 Ad/BMP-2로 유전자 전달된 세포군은 95%의 신생 당단백 생성 증가가 있었다. (p<0.05) 제 1, 2형 아텔로콜라젠에 배양되고 Ad/TGF-β1, Ad/BMP-2로 유전자 전달된 세포군은 제 1, 2형 교원질 mRNA발현증가가 있었으나 (p<0.05) 어떠한 경우에도 osteocalcin mRNA발현은 없었다. 결론: 추간판 세포를 아텔로콜라젠 지지체에서 배양하고, Ad/TGF-β1, Ad/BMP-2으로 유전자 치료를 시행하여 효과적으로 연골형 기질을 생성을 유도할 수 있었다.
펄스 레이저 증착법으로 제조된 인산칼슘 박막의 Ca-free Hanks' Balanced Salt Solution 내에서의 용해도
김혜리,한중석,김영선,이원준,강성근,권언혜 한국생체재료학회 2007 생체재료학회지 Vol.11 No.2
Dissolution behaviors of calcium phosphate films in Ca-free Hanks’ balanced salt solution were studied. Calcium phosphate films were prepared by using pulsed laser deposition (PLD) technique with a KrF excimer laser (248 nm, 2 J/cm2) under 0.25 torr of H2O at a substrate temperature of 600 oC. Sintered hydroxyapatite was used as the target. The films were steam sterilized, and then immersed in Ca-free Hanks’ balanced salt solution (HBSS) for different periods. During immersion in Ca-free HBSS, the mass of the specimen increased for 1 day before it continuously decreased. The Ca/P ratio rapidly decreased to 1.6 for 1 day and then they remained almost the same for the rest of test period. The increase of the mass at the early stage of immersion was due to the dissolution of impurity phases with high Ca/P ratios. Ca ions supplied by the partial dissolution of the film were combined with PO43- and OH- ions in Ca-free HBSS, resulting in the precipitation of calcium phosphate. The decrease of the mass after 1 day was explained by the continuous dissolution of the film. After 7 days, the mass of the PLD calcium phosphate film decreased to 70% of the original mass of the 1-m-thick PLD film.