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이온 빔 조사 처리된 키토산 스펀지의 세포적합도에 관한 연구
구영,Ku, Young 대한치주과학회 1998 Journal of Periodontal & Implant Science Vol.28 No.2
Chitosan is a biodegradable and non-toxic material with a molecular weight of 800-1,500Kd which can be obtained in various forms with extraordinary chemical structures and biological characteristics of which enables it to be used in many fields as a biomaterial. Ion irradiation is a useful tool to modify chemical structures and physical properties of high molecular weight polymers. The basic hypothesis of this study is that when surface properties of chitosan in a sponge form are modified with ion beam-irradiation and cell adhesion properties of chitosan would improve and thereby increase the regenerative ability of the damaged bone. The purpose of this study was to illuminate the changes in the cytocompatibility of chitosan sponges after ion beam-irradiation as a preliminary research. Argon($Ar^+$) ions were irradiated at doses of $5{\times}10^{13}$, $5{\times}10^{15}$ at 35 keV on surfaces of each sponges. Cell adhesion and activity of alkaline phosphatases were studied using rat fetal osteoblasts. The results of this study show hat ion beam-irradiation at optimal doses($5{\times}10^^{13}\;Ar^+\;ion/cm^2$) is a useful method to improve cytocompatibility without sacrificing cell viability and any changing cell phenotypes. These results show that ion beam-irradiated chitosan sponges can be further applied as carriers in tissue engineering and as bone filling materials.
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한국인 Actinobacillus actinomycetemcomitans 균주의 특이 독성 clone형과 혈청형 및 백혈구독성과의 관계
구영,Ku, Young 대한치주과학회 1995 Journal of Periodontal & Implant Science Vol.25 No.3
Previous studies have demonstrated that not all A. actinomycetemcomitans produced significant level of leukotoxic factor and its leukotoxicity have associated with serotype and genetic variation. Our aim was to investigate on the interrelationship between serotype and leukotoxicity of an A. actinomycetemcomitans consisting of 13 clinically well characterized. Korean isolates and to evaluate if particular virulent clonal types of A. actinomycetemcomitans are associated with periodontal disease. For this study, 13 strains of A. actinomycetemcomitans from 6 patients with periodontal disease were isolated and identified by using a selective medium(tryptic soy agar supplemented with 10% serum, $75{\mu}g$ of bacitracin and $5{\mu}g$ of vancomycin per ml) in 10% C02 incubator for 3days with routine Gram staining, colony morphology and biochemical test..For serotyping, antisera were prepared from reference strains of 5 serotypes. (ATCC 29523,Y4, SUNY aB 67, IDH 781, IDH 1705) and then ammonium sulfate precipitation, immunoabsorption and indirect immunofluoroscent procedures were done. For analysis of leukotoxicity, sonic extract of A. actinomycetemcomitans exposed to PMN, and trypan blue was stained for counting the cell viability. Finally Southern blot analyses of genomic DNA digested with the restriction enzyme Tag I was done and the Southern blots were hybridized with the 530bp fragment, termed delta 530, originating from the ltx promoter of strain 652 and deleted from strain JP2. Also ltxA-3.1 and SC2 probe from strain JP2 were hybridized with genomic DNA fragments. Results reveal that strains isolated showed approximately equal proportions of 3 serotypes(b, d, e) and serotype b was not detected. 2 patients harbored 2 different serotypes in the same disease site. The prevalence of leukotoxic strain was 23% and there was no relationship between serotype, leukotoxicity and clinical observations. Especially virulent clonal types of Actinobacillus actinomycetemcomitan (JP2 strain) could not found. Further studies are necessary on the genetic polymorphism of leukotoxin and its relations to clinical status.
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혈소판유래 성장인자 함유 흡수성 차폐막이 치주조직의 재생에 미치는 영향
구영,김정은,한수부,정종평,박윤정,이승진,권영혁,Ku, Young,Kim, Jeong-Eun,Han, Soo-Boo,Chung, Chong-Pyoung,Park, Yoon-Jeong,Lee, Seung-Jin,Kwon, Youg-Hyuk 대한치주과학회 1997 Journal of Periodontal & Implant Science Vol.27 No.1
PDGF-BB has been recognized as a highly potential growth factor for guided tissue regeneration in periodontal defect. This study carried out histologic and histometric evaluation of $200ng/cm^2$ PDGF-BB loaded bioresorbable membrane made from polyglycolic and polylactic acid. It was tested for its biocompatibility, ability to prevent epithelial downgrowth and amount of periodontal regeneration. Without membrane and PDGF-BB unloaded bioresorbable membrane were used as control. Healthy six beagle dogs were used. Each dog was anesthetized and buccal flaps were reflected in the mandibular and maxillary premolar areas. Buccal alveolar bone between the mesiobuccal and distobuccal line angles was surgically removed on the lower 2nd and 4th premolar in mandible, 2nd premolar in maxilla, to a level 4mm apical to the cementoenamel junction with creating a Class II buccal furcation defect for available space. Care was taken not to remove the root cementum layer and rubber impression materials were placed over each surgically created defect. Flaps were repositioned and sutured. Reconstructive surgery was performed 1 month after defect preparation. PDGF-BB loaded membranes and controls were randomly placed on maxillary 2nd premolars and mandibular 2nd and 4th premolars. Plaque control regimen was instituted with daily brushing with a 0.1% chlorhexidine digluconate during experimental periods. The animals were sacrificed 2 and 5 weeks after surgery and undecalcified specimens were prepared for histologic evaluation. The degree of coronal regrowth of new bone, new cementum and the amonut of new bone areas formed on the defected area of the PDGF-BB loaded membrnae turned superior to without membrane and drug unloaded membrane. Experimental membrane could prevent the epithelial downgrowth irrespective of drug loaded or not and showed good biocompatiblity, These results implicated that PDGF-BB loaded bioresorbable membrane could be highly useful tool for guided tissue regeneration of periodontal defects.
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습답도작기술향상에 관한 연구 2 : 지하수가 시비량과 재배밀도를 달리한 수도의 생육 및 수량에 미치는 영향
具英書,盧承杓,黃昌周,羅種城,李畯培,鄭鎭昱 韓國作物學會 1976 한국작물학회지 Vol.21 No.1
1. 암거배수구에서는 최고분얼기이후 1일의 32mm 수직배수 실시로 인한 양분용탈현상으로 초기 생육은 다소 부진한 경향이였으나 배수실시 15~20일후부터는 배수효과로 후기 생육 증대를 보였다. 2. 최고분얼기가 배거배수 처리구는 7월 9일, 무배수처리구는 7월 15일로 무배수가 약 1주일간 늦어 지발분얼로 인한 유효경비율저하를 초래하였다. 3. 영화수는 암거배수구보다 무배수구가 높았고 암거배수구무배수구 모두 다비밀식일수록 증가경향이였다. 4. 수도의 고위다수확을 위한 수수는 400/m2 이상 영화수는 4만립/m2 이상을 확보해야 된다면 암거배수시설을 할 경우 시비량 N25kg/10a 이상 재식밀도 100 /3.3m2 이상의 다비밀식 재식법도 고려해 볼만하겠다. 5. 평균 일수영화수와 출수기의 엽면적지수와는 암거배수 모두 고도의 정상관관계가 인정되였다. 6. 배수조건별간경엽중의 무기성분함량을 보면 N,K은 최고분얼기를 극대로 출수기 성숙기 이후는 계속감소하였고 무배수구가 암거배수구보다 다소 높은 경향이며 Ca은 최고분얼기 이후 다소 증가하였고 그 이후는 거의 일정치을 유지하였으며 Mg은 암거배수구가 무배수구보다 높은 경향을 보여 N와는 대조적이었다. 7. 출수기부터 성숙기까지의 NAR은 암거배수구가 월등히 높았고 밀식일수록 증가 경향이였으며, 엽록소 함량은 무배수구가 높고 다비일수록 증가경향에었다. 8. 근중은 암거배수 처리에서는 시비량 증가에 따라 증가하였으나 무배수구에서는 일정한 경향이 없었으며 심층의 뿌리 분포량도 무배수구보다. 암거배수구가 많은 경향이였다. 9. 뿌리의 α -Na산화력은 암거배수구가 각처리 모두 높았고 일정면적당 근활력은 다비밀식일수륵 증가경향이었으며 무배수구에서는 N20kg과 80주처리구가 높은 경향이었다. 10. 뿌리의 α -Na산화력과 등숙비율은, 암, 무배수구 모두 고도의 정상관 관계가 인정되였고 수량 및 순동화율과는 암거배수구에서 만 정상관 관계가 인정되었다. 11. 이상의 결과 10α 당 수양은 암거배수구가 무배수구보다 배수조건별간에는 21%증수, 시비량간에는 15kg, 처리에서는 2%, 20kg처리에서는 7%, 25kg 처리에서는 20%의 높은 증수율을 보였고 재식밀도간에는 암거배수구가 60주 처리에서는 5%, 80주처리에서는 4%, 10주처리에서는 15%의 증수율을 보였다. Marine deposite soil was studied to evaluate morohological changes of paddy rice under subdrainage control. The content of organic and inorganic mate rial were low at high temperature in the mid-summer. This soil lacked air and accumulated more H2 S. Thus rice root was rotted with high infection of virus and insect and yield was relatively low.
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