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Artificial Metalloproteases with Broad Substrate Selectivity Constructed on Polystyrene
고은화,서정헌 대한화학회 2004 Bulletin of the Korean Chemical Society Vol.25 No.12
Although the proteolytic activity of the Cu(II) complex of cyclen (Cyc) is greatly enhanced upon attachment to a cross-linked polystyrene (PS), the Cu(II)Cyc-containing PS derivatives reported previously hydrolyzed only a very limited number of proteins. The PS-based artificial metalloproteases can overcome thermal, mechanical, and chemical instabilities of natural proteases, but the narrow substrate selectivity of the artificial metalloproteases limits their industrial application. In the present study, artificial metalloproteases exhibiting broad substrate selectivity were synthesized by attaching Cu(II)Cyc to a PS derivative using linkers with various structures in an attempt to facilitate the interaction of various protein substrates with the PS surface. The new artificial metalloproteases hydrolyzed all of the four protein substrates (albumin, myoglobin, γ- globulin, and lysozyme) examined, manifesting kcat/Km values of 28-1500 h−1M−1 at 50 oC. The improvement in substrate selectivity is attributed to steric and/or polar interaction between the bound protein and the PS surface as well as the hydrophobicity of the microenvironment of the catalytic centers.
‘보호관찰관의 휴대전화 검사 감독’ 실무 적용 방안 및 처우 모델 개발 연구 - 보호관찰 청소년의 재범 방지를 위한 지도・감독 방안 -
성의찬,고은화,최동완 한국보호관찰학회 2023 보호관찰 Vol.23 No.2
본 연구는 소년보호관찰 대상자의 스마트폰 관련 범죄가 증가함에 따라 효과적인 재범 방지 대책으로 보호관찰관의 스마트폰 SNS 등 생활에 대한 지도・감독 방안을 모색하고 처우 모델을 개발하고자 하였다. 연구는 크게 3가지 주제로 진행되었다. 먼 저 보호관찰 청소년의 스마트폰 중독과 공격성 관련성을 확인하고 조절 변인으로 자 기효능감 매개효과를 검증하였다. 두 번째는 사이버폭력 경험이 있는 대상자를 선별 하여 자기조절 향상 집단 프로그램을 실시하였고 효과성을 확인하였다. 세 번째로 보호관찰관이 소년보호관찰 대상자를 지도하는 과정에서 스마트폰 SNS 등 생활보고 를 하도록 하고 심층 개입을 통해 대상자를 처우하는 모델을 개발하고자 하였다. 연 구 결과, 스마트폰 중독 수준이 보호관찰 청소년의 공격성에 미치는 영향이 확인되 었고 스마트폰 중독이 언어적 공격성과 분노에 미치는 영향에서는 자기효능감이 완 화시키는 역할을 하는 것으로 나타났다. 또한 집단 프로그램 실시와 보호관찰관의 스마트폰 SNS 등 생활 감독에 적극 개입한 결과, 대상자의 불법행동 사전 차단 및 긍정적 행동변화에 기여한 바가 큰 것으로 나타났다. 본 연구는 처음으로 보호관찰 청소년을 대상으로 스마트폰 중독과 공격성 관련성을 검증하였다는데 의의를 가지며 소년보호관찰 대상자의 재범 방지를 위한 차별적인 관리방안을 제언하였다. As the number of smartphone-related legal crimes of juvenile probation targets increases, this study sought to find ways to guide and supervise probation officers’ lives such as smartphone SNS as an effective countermeasure to prevent recidivism. The study was largely conducted on three topics. First, the relationship between smartphone addiction and aggression of probation adolescents was confirmed, and the mediating effect of self-efficacy was verified as a control variable. Second, the self-regulation improvement group program was newly established by selecting subjects with cyber violence experience, and the effectiveness was confirmed. Third, in the process of guiding juvenile probation victims, probation officers were required to report their lives, such as smartphones and SNS, and a model for treating the target company through in-depth intervention was developed. As a result of the study, the effect of smartphone addiction level on the aggression of probation adolescents was confirmed, and self-efficacy played a role in alleviating the effect of smartphone addiction on verbal vacancy and anger. In addition, it was found that they actively intervened in life supervision such as smartphone SNS of group programs and protection officers, and contributed greatly to the pre-blocking of illegal behavior and positive behavior change of the subject. This study was significant in that it was the first time to develop a relationship between smartphone addiction and aggression in probation adolescents, and suggested a differential management plan to prevent of recidivism of juvenile under probation.
천성수,고은화,손애리,조정애,이상숙 알코올과 건강행동학회 2007 알코올과 건강행동연구 Vol.8 No.-
The aim was to identify a relationship between the JOHARI Window and patterns of alcohol use and alcohol-related problems among college students. Four colleges were selected randomly, which were located in Seoul in 2000. In conducting the data analyses, 483 cases were used. For measuring alcohol-related problems among college students, we used NAST (1), AUDIT, US National Alcohol Survey, and Social Value Scale of College Drinking, as scales. The Johari Window was classified by Open Quadrant Type, Blind Quadrant Type, Hidden Quadrant Type, and Unknown Quadrant Type. The major findings are as follows: First, there are the highest portions of alcohol users (63.9%) and binge drinkers (62.1%) among the Open Quadrant Type students, and there is the lowest portion of alcohol users (51.4%) and binge drinkers (54.9%) among the Unknown Quadrant Type students. Second, the Open Quadrant Type and Blind Quadrant Type students are more likely to have unwanted drinking episodes, and the Hidden Quadrant Type students are less likely to have unwanted drinking episodes. Third, alcohol dependent students (NAST1) are 50.5% among the Open Quadrant, 46.9% among the Unknown Quadrant, 35.7% among the Blind Quadrant, and 12.3% among the Hidden Quadrant Type students. Fourth, the proportion of Alcohol Use Disorder students is 52.4% among the Open Quadrant, 50.0% among Blind Quadrant, 46.9% among the Hidden Quadrant, and 41.8% among the Unknown Quadrant Type students. Fifth, the Open Quadrant students drink the most, but the Blind Quadrant students have the highest portion of alcohol-related problems. In conclusion, the Blind Quadrant Type students have the most severe alcohol problems, and the Open Quadrant Type students have more severe alcohol problems than the Hidden Quadrant and the Hidden Quadrant Type students. The Hidden Quadrant and the Unknown Quadrant Type students are more likely to have positive attitudes toward drinking than the other types, but are less likely to have alcohol-related problems.