홍삼 에탄올 추출물의 생리활성과 세포증식 효과

황성연,안성훈,Hwang, Sung-Yeoun,Ahn, Seong-Hun 대한약침학회 2011 Journal of pharmacopuncture Vol.14 No.3

Objectives: Reactive Oxygen Species(ROS) are continuously produced at a high rate as a by-product of aerobic metabolism. Since tissue damage by free radical, ROS such as hydrogen peroxide($H_2O_2$), nitric oxide(NO) increases with age. Several lines of evidence provided that ROS appears to cause to develop aging-related various diseases such as cancer, arthritis, cardiovascular disease. In this study, we have conducted to investigate the pharmacological effects of red ginseng for the development possibility to pharmacopuncture drug sources or healthy aid foods. Methods: For our aims, it was investigated the biological activities of Red Ginseng ethanol extracts (RGEE) by measuring total polyphenol contents, total flavonoid contents, DPPH radical scavenging activity, ABTS radical scavenging activity and cell viability of MCF 10A and SK-MEL-2 in vitro with MTT assay method. Results: The total polyphenol contents of RGEE was 3.06${\pm}$0.11mg/g in 10mg/ml, the total flavonoid contents of RGEE was 1.35${\pm}$0.01mg/g in same concentration. The ABTS radical scavenging activity was about 80% and that of DPPH activity was 65% in 50mg/ml of RGEE. The cell viability of SKMEL-2, skin cancer cell line was decreased and that of MCF 10A, skin normal cell line was increased. Conclusions: We conclude that RGEE may be useful as potential functional foods or pharmacopuncture drug sources on the diseases induced by oxidant stress.

저온숙성 반죽이 바켓의 품질에 미치는 영향

황성연,김영만,조대희 한국식품영양학회 1998 韓國食品營養學會誌 Vol.11 No.4

바켓반죽의 저온숙성이 바켓의 품질에 미치는 영향을 조사하였다. 글루텐을 완전하게 발전시킨 반죽을 세등분하여 즉시 성형하여 구운 것과 1∼-1℃에서 각각 24, 48시간 저온숙성시키고 발효 및 저온숙성 중의 pH변화와 유기산 종류 및 그 함량을 분석하였다. 바켓 발효과정중 생성되는 유기산은 acetic acid가 가장 많았으며 다음은 lactic acid였으며 저온숙성 중 pH변화는 극히 적었다. 각각의 시료를 20℃에서 저장보관하면서 DSC를 사용하여 crumb의 enthalpy 변화로 바켓의 노화 정도를 측정한 결과 반죽의 저온숙성 기간의 차이는 제품의 노화에 영향을 거의 미치지 않았으며 종류별로 구워낸 바켓의 저장중에 enthalpy가 증가하여 노화도에 차이를 보였다. 한편 관능검사의 결과는 2시간 발효시킨 바켓의 외관이 가장 좋았다. 그러나 맛, 조직감, 향미 등은 24시간 저온숙성시킨 것이 다른 것에 비하여 높은 평점을 받았다. This study was conducted to investigate influences of cold aged dough on the quality of bagutte. After 2 hours fermenting, the dough was divided 2 parts and they were stored in the refrigirator adjusted 1∼-1℃. The cold aging time was 24, 48 hours respectively. After 2 hours fermentation, the dough's pH was 5.45 and 24, 48 hours cold aged dough were 4.99, 4.81. During cold aging, the difference of pH was not significant. The main organic acids in the baguette were lactic acid, acetic acid and malic acid. Among these organic acids, the acetic acid was the largest quantity regardlessly in different fermentation and the cold aging time. Right after baking, the enthalphy of all samples were almost same even though in different cold aging time, but during different storage periods they showed the difference of retrogradation by the increase of the enthalphy. 24 hours cold aging baguette had the best taste, aroma and texture in the bread's score sheet.

가속도 값 변화에 따른 지능센서(HH)의 센싱능력 평가

황성연,홍동표,김홍건 한국공작기계학회 2004 한국생산제조학회지 Vol.13 No.1

A new method that estimates the sensing ability of HH smart sensor is proposed. The new signal processing method have been developed that can distinguish among different materials relatively. The HH smart sensor was developed for recognition of materials. The HH s& sensor was made for experiment. Then, it was estimated the ability to recognize objects according to acceleration value. The sensing ability of smart sensor has been estimated with the R_(SAI) method. Experiments and analysis were executed to estimate the ability to recognize objects according to acceleration value changing. Dynamic characteristics of HH smart sensor were evaluated relatively through a new R_(SAI) method that uses the power spectrum density. Applications of this method are for finding abnormal conditions of objects (auto-manufacturing0, feeling of objects (medical product), robotics, safety diagnosis of structure, etc.

지적센서의 형태에 따른 센싱능력 평가

황성연,홍동표,강희용 한국공작기계학회 2001 한국생산제조학회지 Vol.10 No.4

In this paper, we will propose the new method that estimates the sensing ability of smart sensor. A study is estimation method that evaluates the sensing ability about smart sensor respectively. According to acceleration (g) and displacement changing, we estimated the sensing ability of smart sensor using the SAI(Sensing Ability Index) method respectively. We made the smart sensors in our experiment. The types of smart sensor are three types (H1,H2.H3 smart sensor). The smart sensors were developed for recognition of materials. Experiments and analysis were executed to estimate the sensing abili-ty of smart sensor. Dynamic characteristics of smart sensors(acceleration changing) were evaluated respectively through a new method(SAI) that uses the power spectrum density.

색소지(色素地)와 화장토(化粧土) 제조에 관한 시찰(試察)

황성연 이화여자대학교 도예연구소 1993 陶藝硏究 Vol.- No.15

Effect of the Mesoporous Structure of Titanium Silicate (TS-1) Zeolite on the Melting Behavior and Isothermal Crystallization Behavior of Poly(butylene succinate)/TS-1 Zeolite Hybrid Composites

황성연,임승순,윤원재,유의상 한국고분자학회 2012 Macromolecular Research Vol.20 No.10

In this study, we investigated the effects of mesoporous titanium silicate (TS-1) zeolite on the melting behavior and isothermal crystallization in poly(butylene succinate) (PBS)/TS-1 zeolite hybrid composites (PTHC). Isothermal crystallization results revealed that TS-1 zeolite acted as a nucleation agent in PTHC, thus the t1/2 of PTHC was faster than that of homo-PBS. However, the nucleation effect of TS-1zeolite did not depend on the TS-1zeolite content. The large surface area of the mesoporous structure readily formed molecular chains inside and outside of the pore mouths of TS-1 zeolite, covering the nucleation site, as a result of the byproduct deposition during polymerization. At isothermal crystallization, temperatures ranging from 88 to 92 oC, nucleation of TS-1 zeolite occurred because of the presence of free byproducts and the formation of a molecular chain in the pore mouths. In contrast, isothermal temperatures ranging from 80 to 84 oC resulted in ineffective nucleus activation because of the steric hindrance in the porous structure. Synchrotron small-angle X-ray scattering (SAXS) analysis revealed that TS-1 zeolite can accelerate lamellar recrystallization during heating.

Effects of Clay Modification on Crystallization Behavior, Physical, and Morphological Properties of Poly(butylene terephthalate) Nanocomposites

황성연,Khaydarov A.A.,박준영,유의상,임승순 한국고분자학회 2011 Macromolecular Research Vol.19 No.7

Poly(butylene terephthalate) (PBT) nanocomposites of various clay types and contents were prepared by in situ polymerization. This study examined the effect of surface urethane modification of clay (30BM) on the mechanical properties and isothermal and nonisothermal crystallization behavior of modified clay compared to pristine clay (Na) and alkyl modified clay (30B) nanocomposites. The PBT/30BM nanocomposites exhibited increased mechanical properties due to the enhanced affinity between the clay and PBT matrix. In the case of the PBT/Na nanocomposites, pristine clay can retard the crystallization rate due to steric hindrance of the narrow silicate layers. The PBT/30BM nanocomposites exhibited the fastest crystallization rates of all the samples due to wide gallery spacing and strong interactions between the 30BM and PBT molecules that lead to a nucleation effect during the crystallization process. Polarized optical microscopy showed that the spherulites in the PBT/30BM nanocomposites became smaller and increased the nuclei density at the designated crystallization temperature compared to Homo PBT.

Fabrication of Superabsorbent Ultrathin Nanofibers Using Mesoporous Materials for Antimicrobial Drug-Delivery Applications

황성연,윤원재,윤성훈,유의상,김태희,임승순 한국고분자학회 2013 Macromolecular Research Vol.21 No.11

The goal of this study was to prepare functionalized, ultrathin nanofibers using mesoporous materials (TS-1 zeolite) with maximum capability to both absorb drug and control drug release (these fibers are herein referred to as PZ-01); a second goal was to prepare nanofibers that exhibit biodegradability after drug release. Under optimal conditions, the characteristic of TS-1 zeolite enabled the preparation of ultrathin nanofibers with diameters below 100 nm, or one-twentieth the size of homo-poly(butylene succinate) (PBS) fibers. In addition, PZ-01 nanofibers exhibited high drug loading capacity compared to homo-PBS fibers. This result was attributable to the large surface area of ultrathin nanofibers and to the strong ionic interaction between hydrophilic drugs and the metal ions of TS-1 zeolite. In vitro cytostatic assay indicated that prepared PZ-01 has cytostatic action toward both Gram-positive and Gram-negative bacteria. The excellent drug wetting behavior of PZ-01 led to longer drug-release times. After drugrelease tests, antibacterial tests confirmed that homo-PBS fibers had diminished antibiotic activity regardless of the type of bacteria, whereas the antibacterial activity of tested PZ-01 was highly efficient against both Gram-positive and Gram-negative bacteria. In the cell viability test, PZ-01 exhibited a greater decrease in cytotoxicity and an increase in cell viability compared with the homo-PBS nanofiber. Based on this research, we anticipate that these materials will be promising candidates for biomedical applications such as biofilters for microbes, wound dressings, and drugdelivery products.

A comparison with Characterization between PET/pristine MMT and Modified MMT Nanocomposites by using in-situ polymerization

황성연,임승순 한국고분자학회 2007 한국고분자학회 학술대회 연구논문 초록집 Vol.2007 No.4

기후위기, 사례별 기록으로 보는 예측 가능한 미래

황성연 한국물환경학회 2023 공동춘계 학술발표회 논문집 Vol.2023 No.0