천연식용색소 개발을 위한 치자 황색색소의 정제

김희구,이상준 한국식품영양학회 1998 韓國食品營養學會誌 Vol.11 No.1

현재 사용 중인 합성색소 중에서 사용빈도가 가장 높은 합성색소 중의 하나인 황색4호의 대체용 천연색소를 치자에서 추출하였으며 추출조건은 추출온도 70℃, 추출시간 48시간, 추출 pH 7.0 및 기질농도는 10%였으며, 이 때의 추출수율은 61%였다. 추출한 조색소를 정제하기 위하여 활성백토를 사용하여 정제를 실시하였으며, 조색소액에서 배당체 성분과 색소성분을 각각 단일 peak로 분리하였다. 조색소액을 정제한 후 정제도는 약 27배 증가하였고, 수율은 96%로 높게 나타났다. In order to make natural food color from gardenia(Gardenia jasminoides), we investigated optimal conditions of color extraction, in case of water extraction, optimal conditions for color extraction were 70℃, 48hrs, pH 7.0 and substrate 10%, respectively. And extracted crude color was purified by activated white clay, were isolated glycoside peak(238nm) and yellow color peak(440nm) from extracted crude color. The purified color was increased by 27-fold and the yield was 96%.

철근 콘크리트 구조와 철골 구조 아파트의 비교 관찰

金熙球 홍익대학교 산업기술연구소 1997 産業技術 Vol.7 No.-

This is a comparative study between r.c and s.s apartment of 15 stories. (1) The cost of construction of s.s apartment is 40-50% expensive comparing with that of r.c apartment. (2) The utility of inside space of s.s apartment is better than that of r.c apartment. (3) The unit height of story of r.e apartment is 2.6 - 2.8m, but that of s.s apartment is 3.3m. (4) The easiness of construction are same for both apartments. (5) We need more studies about economy of two apartment.

Littoral Cell Angioma (LCA) Associated with Liver Cirrhosis

김희구,박인서,이정일,정석,이진우,권계숙,이돈행,김범수,김형길,신용훈,김영수,안인선,이건영 연세대학교의과대학 2005 Yonsei medical journal Vol.46 No.1

A littoral cell angioma (LCA) is a rare benign vascular tumor of the spleen. A 60-year-old man, with multiple nodules in imaging study and liver cirrhosis graded as Child-Pugh classification class A, was transferred for splenomegaly. A thrombocytopenia was found on hematological evaluation. Because there was no evidence of hematological and visceral malignancy, a splenectomy was performed for a definitive diagnosis. The histological and immunohistochemical features of the splenic specimens were consistent with a LCA. After the splenectomy, the thrombocytopenia recovered to the normal platelet count. There has been no previous report of a LCA combined with liver cirrhosis. Herein, the first case of a LCA in Korea, diagnosed and treated by a splenectomy, is reported.

경량 조립식 연속난방시스템의 효율성 고찰

金熙球 弘益大學校 1991 弘大論叢 Vol.23 No.2

This is a study on efficiency of light weight sectional continuous heating system. Comparing with usual intermittent heating system(heavy ondol system), light weight sectional continuous heating system has some of efficiency and advantages as follows. 1) We can save 26% of reinforcing steel bar in two way slab, 7%~10% in one way slab of apartment, 3%~7% in footing, and 15% of concrete mass in footing. 2) We can save total cost of construction because of ready-made heating system. 3) It gives better utility of land for us because of lower height of story.

치자 황색색소에 대한 변이원성 시험

김희구 한국식품영양학회 1998 韓國食品營養學會誌 Vol.11 No.1

세균을 이용한 복귀돌연변이 시험계는 Salmonella typhimurium 히스티딘 요구성 변이주를 이용하여 염기대치환(base change)의 돌연변이를 검출하는 S. typhimurium TA100, TA1535와 frameshift형의 돌연변이를 검출하는 S. typhimurium TA98, TA-1537로 구성되어 있으며, 화학물질의 변이원성에 의하여 히스티딘 비요구성으로 복귀하는 돌연변이를 검토하는 시험계로 널리 이용되고 있다^13). 시험결과, Yellow pigment는 S. typhimurium에 대한 독성도 나타나지 않았으며, 배지 1ml당 200㎍ 이하의 모든 농도에서 S-9 Mix(대사활성계)의 유무에 관계없이 S. typhimurium 모든 변이주에서 히스티딘 요구성에 대한 복귀돌연변이를 유발하지 않는 물질로 판단되었다. 이상의 결과로부터 치자황색색소는 S. typhimurium을 이용한 복귀돌연변이시험에서 유전독성이 없는 물질로 사료되었다. Gardenia yellow pigment produced by Gardenia jasminoides Ellis was tested for reverse mutagenic test in Salmonella typhimurium strains TA1535, TA1537, TA98 and TA100 at concentrations raging from 6.25 to 200 ㎍/ml per plate. No significant reverse mutagenic activity was observed in any of the S. typhimurium strains, in either presence or absence of S9 mix. There was no toxicity to the bacteria. These result indicate that yellow pigment doesn't have mutagenicity.

웨브 개구부 합성보의 휨전단 거동에 관한 실험적 연구

金熙球,感昌湖 홍익대학교 산업기술연구소 2000 産業技術 Vol.10 No.-

Nine tests to failure are performed on full-scale eight composite beams with unreinforced web opening having ribbed slabs with formed deck which are perpendicular to the steel section and one steel beam. The effects of slab width, reinforcing of stud, moving of rib, moment-shear ratio are studied. At the low M/V ratio, Vierendeel action around the high moment end of the opening is occurred and the large deflection across the opening and transverse cracking are occurrred with increasing of applied load. As the M/V ratio increases, the relative deflection across the opening decreases. And at failure, full tensile strain are occurred at bottom T section of steel beam, and concrete crushes at the High Moment End of the opening. With narrow slabs, diagonal tension failure at the high moment end of the opening is occurred. And with wide slabs, rib punch-through failure is occurred near the high moment end of the opening. The implications for design are discussed.

물리적 처리에 의한 한천의 물리화학적 및 유통 특성

김희구,김옥도,손홍주 한국식품영양학회 1997 韓國食品營養學會誌 Vol.10 No.2

다양한 생리적 기능을 가지며 식품뿐만 아니라 광범위한 분야에서 이용되고 있는 한천의 용융점을 인위적으로 낮추어줌으로서 그 이용성을 간편화하기 위하여 분무건조 및 압출성형과 같은 처리방법이 물리화학적 및 유동특성에 미치는 영향에 대하여 조사하였다. 일반 한천, 분무건조한천 및 압출성형한천의 겔강도는 각각 1.070, 850, 523.28g/cm^2로 압출성형한천에서 가장 낮았다. 제조방법을 달리한 한천의 용해도가 상승하기 시작하는 개시온도와 완료온도는 일반한천에서 각각 80과 110℃, 분무건조한천에서 60과 90℃ 그리고 압출성형한천에서 35와 80℃로 압출성형한천에서 가장 낮았다. 75℃에서의 점도변화는 일반한천에서 170.50cps, 분무건조한천에서 17.07cps 그리고 압출성형한천에서 12.81cps로 압출성형한천이 가장 낮았다. 용융점과 응고점은 일반한천이 110.17과 40.52℃, 분무건조한천이 90.05와 39.54℃ 그리고 압출성형한천이 80.01과 36.05℃로 압출성형한천이 가장 낮았다. The effects of spray drying and extrusion drying on functional properties of agar were investigated to increase the availability of agar in food industry. In case of unmodifed and spray-dried agar, gel strength and viscosity were 1,070g/cm^2 and 170.50cps, 850g/cm^2 and 17.07cps, respectively, while 523.28g/cm^2 and 12.81cps in extrusion-dried product. The lowest dissolving onset and conclusion temperature were revealed in extrusion sample, where it was attained in 80 and 110℃ of unmodified, 60 and 90℃ of spray-dried, and 35 and 80℃ of extrusion-dried agar, respectively. Melting and settion in unmodified sample were showed to 110.17 and 40.52, 90.05 and 39.54℃ in spray dried, and 80.01 and 36.05℃ in extrusion-dried agar, respectively.

천연식용색소 개발을 위한 치자에서 황색소의 추출

김희구,손홍주 한국식품영양학회 1997 韓國食品營養學會誌 Vol.10 No.2

천dus식용색소를 제조하기 위하여 치자를 이용하여 색소추출의 최적조건과 황색 4호와의 내열성 및 내광성을 조사하였다. 에탄올을 추출용매로 사용한 경우 색소추출 최적조건은 추출온도 40℃, 추출시간 42시간, 추출 pH 7.0 및 기질농도 10%였다. 물추출의 경우 추출온도는 70℃, 추출시간 48시간, 추출 pH 7.0 및 기질농도 10%였다. 최적조건에서의 추출수율은 에탄올의 경우 75%였고, 물추출의 경우는 63%로 나타났다. 황색4호와 치자 황색소의 내열성 및 내광성을 비교 검토한 결과, 황색 4호는 내열설과 내광성이 모두 98%이상으로 나타났으나, 치자 황색소의 경우는 내열성은 62%, 내광성은 90%로 나타났다. In order to make natural food color from Gardenia, we invsetigated optimal conditions of color extraction, and thermal stability and light stability of color extracted compared with Yellow-4. In case of ethanol extraction, optimal conditoins for color extraction were substrate 10%, 40℃, pH7.0 and 42hrs, respectively. In case of water extraction, optimal conditions for color extraction were substrate 10%, 70℃, pH 7.0 and 48hrs, respectively. Extraction yield in the optimal conditons was 75% in ethanol and 63% in water. The thermal stability and light stability of Yellow-4 were both upper 98%, but those of Gardenia yellow color were 62 and 90%, respectively.

대장에 발생한 대세포 신경 내분비 암

김희구,최윤아,박현주,최새로운,손동욱,김철현,신용운 대한내과학회 2008 대한내과학회 추계학술대회 Vol.2008 No.-

한국 초고층 아파트 내력벽식 건물의 내진성 고찰

金熙球 홍익대학교 산업기술연구소 1996 産業技術 Vol.6 No.-

This is a study on seismic design of high rise bearing wall apartment buildings of Korea. The study about earthquake pattern of Korean peninsula is too short to make clear about activity of faulting. So seismic design factor of Korea is a kind of possibilism. If seismic regional factor 0.12 increase to 0.24 of west side of U.S.A(the circus-pan-Pactific earthquake belt)about 23 story bearing wall apartment building, we can have following results. (1) The area of reinforcing bar of bearing wall must be increased as 4.32 for under 9 story to that by Korea seismic code. (2) In case of 9 story to 19 story, the area of bar must be incerased as 4.34 comparing that of Korea seimic code. (3) In case of 20 story to 23 story, the area of bar must be incerased as 1.25 comparing that of Korea seismic code. (4) Total average increasing area of bar is 3.03 times comparing that of Korea seismic code.