의료의 질 향상을 위한 병원정보화 전략 : 임상의학분야

곽연식,전희선,Gwak, Yeon-Sik,Jeon, Hui-Seon 한국의료질향상학회 1997 한국의료질향상학회지 Vol.4 No.1

Screening Antifungal and Exceptional Colonization Strains from Nationwide Actinobacteria Library

곽연식,Heeil Do,Da-Ran Kim 한국농약과학회 2022 농약과학회지 Vol.26 No.3

Since the Industrial Revolution, the population has grown exponentially and by 2050, the world's population is expected up to 9 billion. As the population increases, food supply is required to about 1.5 times the current amount, but the occurrence of diseases and environmental stress on crops due to climate change is increasing and crop production is decreasing. Chemical pesticides have contributed greatly to the increase in food production and security. However, due to issues of environmental, eco-friendly management techniques are demanded. There are many plants beneficial microorganisms that have been known to contribute to preventing plant disease occurrence as eco-friendly approaches in lab or greenhouse experimental scales. However, the candidate beneficial microbes failed to protect the plant against pathogens or abiotic stresses in commercial crop cultivated field conditions, due to a lack of colonization ability on plants. Therefore, this study aimed to secure outstanding colonization of microbial strains among Actinobacteria collection nationwide. Of the 2,114 isolates of Actinobacteria, two strains showed aggressive antifungal activity against a wide range of fungal pathogens and exceptional colonization ability in the plant rhizosphere. The strains have an excellent possibility to be biological control agents and members of the synthetic community for plant disease control.

Different oxidative burst patterns occur during host and nonhost resistance responses triggered by Xanthomonas campestris in pepper

곽연식,Ki Soo Han,이정한,이경희,정우식,Kirankumar S. Mysore,권영상,김희규,Dong-Won Bae 한국식물생명공학회 2009 식물생명공학회지 Vol.36 No.3

The hypersensitive reaction (HR) is the mostcommon plant defense reaction against pathogens. HR isproduced during both host- and nonhost-incompatible interactions. Several reports suggest that similarities existbetween host and nonhost resistances. We assayed the patternof generation of reactive oxygen species (ROS) and scavengingenzyme activities during nonhost pathogen-plantinteractions (Xanthomonas campestris pv. campestris/Capsicumannuum L.) and incompatible host pathogen-plant interactions(Xanthomonas campestris pv. vesicatoria race1/Capsicumannuum L.). Both O2- and H2O2 accumulated much fasterduring nonhost resistance when compared to the hostresistance. The scavenging enzyme activities of superoxidedismutase (SOD), catalase (CAT) and peroxidase (POX)were also different during the host- and nonhost-incompatibleinteractions. CAT activity was much higher during nonhost resistance, and several new isozymes of SOD and POX weredetected during nonhost resistance when compared to thehost resistance. Lipoxygenase (LOX) activity was higher inhost resistance than nonhost resistance during the earlystages of infection. Interestingly, the nitric oxide (NO) radicalaccumulated equal amounts during both host and nonhostresistance at early stages of infection. Further studies areneeded to determine the specific pathways underlying thesedifferences between host and nonhost resistance responses.

임상검사실에서의 정도관리

郭然植 대한의학협회 1977 대한의학협회지 Vol.20 No.8

미생물 군집의 이해를 통한 유기농업 작물 건강관리

곽연식 ( Youn-sig Kwak ) 한국환경농학회 2023 한국환경농학회 학술대회집 Vol.2023 No.0

식물은 대부분의 괴사성 병원체에 대해 유전적 저항력을 갖지 못한다. 이를 보완하기 위해, 식물은 미생물 군집에서 유익한 미생물을 동원하여 병원체와 해충으로부터 자신을 방어한다. Pseudomonas, Bacillus, 그리고 Trichoderma는 식물을 다양한 스트레스로부터 보호하는 잘 알려진 식물 프로바이오틱스이다. 2015년 식물마이크로바이옴 이니셔티브는 작물 건강과 생태계 균형에서 식물-미생물 군집의 중요성이 강조되었다. 본 발표에서는 Streptomyces라는 새로운 프로바이오틱스 균주, 식물 및 환경과의 상호작용, 그리고 농업 생태계에 미치는 영향을 소개하고자 한다. 인간이 사용하는 항생물질의 70% 이상이 Streptomyces에서 유래되었다. 그러나 Streptomyces의 식물 및 농업 생태계 상호작용에서의 역할과 중요성은 심도 있는 접근이 이루어지지 않았다. 이를 해결하기 위해 우리는 “농업 연구에서의 Streptomyces (STAR)” 프로젝트를 시작하였다. 이 프로젝트는 다양한 작물 재배 체계에 초점을 맞추었다. 차세대 파이로시퀀싱 플랫폼을 사용하여 작물의 근권, 꽃권, 그리고 내권의 미생물 군집을 분석하였다. 연구를 통해 식물의 뿌리, 꽃, 그리고 내부조직에서 서식하는 세 가지의 가상 프로바이오틱스 Streptomyces 균주를 분리 및 특성화했다. 놀랍게도, 이 세 균주는 서로 다른 서식지에서 발견되었음에도 동일한 유전체 서열을 보였다. 이는 서로 다른 시스템에서 동일한 균주의 존재와 기능에 관한 의문을 제기하였다. 결론적으로, Streptomyces는 작물 생태계에 유익한 효과를 제공하는 널리 분포하는 독특한 미생물이다. STAR 프로젝트는 유기농 농업을 위한 유망한 기회와 가능성을 가지고 있다. Plants lack genetic resistance to most necrotrophic pathogens. To compensate for this, they enlist beneficial microbes from their microbiota to defend against pathogens and pests. Pseudomonas, Bacillus, and Trichoderma are well-known plant probiotics that safeguard plants from various stresses. The Phytobiome initiative, launched in 2015, recognizes the importance of plant-microbe communities in crop health and ecosystem balance. In this presentation, we introduce a new probiotic strain called Streptomyces, its interactions with plants and the environment, and its impact on the agroecosystem. Streptomyces, a gram-positive bacterium, is the source of over two-thirds of antibiotics used in human medicine, yet its role and significance in plant and agroecosystem interactions are underestimated. To address this, we initiated the “STreptomyce in Agriculture Research” (STAR) project, focusing on various cropping systems. We employed advanced pyrosequencing platforms to analyze the microbial communities in the rhizosphere and anthosphere. Through this study, we isolated and characterized three putative probiotic Streptomyces strains that were found to colonize the rhizosphere, anthosphere, and phyllosphere. Surprisingly, these three strains exhibited identical genome sequences despite inhabiting different habitats. This raises questions about the existence and function of identical strains in distinct systems. In conclusion, Streptomyces is a ubiquitous and unique microbe that provides beneficial effects to plants and their phytobiome communities. The STAR project holds promising opportunities and possibilities for organic agriculture.

Take-all of Wheat and Natural Disease Suppression: A Review

곽연식,David M. Weller 한국식물병리학회 2013 Plant Pathology Journal Vol.29 No.2

In agro-ecosystems worldwide, some of the most important and devastating diseases are caused by soil-borne necrotrophic fungal pathogens, against which crop plants generally lack genetic resistance. However, plants have evolved approaches to protect themselves against pathogens by stimulating and supporting specific groups of beneficial microorganisms that have the ability to protect either by direct inhibition of the pathogen or by inducing resistance mechanisms in the plant. One of the best examples of protection of plant roots by antagonistic microbes occurs in soils that are suppressive to takeall disease of wheat. Take-all, caused by Gaeumannomyces graminis var. tritici, is the most economically important root disease of wheat worldwide. Take-all decline (TAD) is the spontaneous decline in incidence and severity of disease after a severe outbreak of takeall during continuous wheat or barley monoculture. TAD occurs worldwide, and in the United States and The Netherlands it results from a build-up of populations of 2,4-diacetylphloroglucinol (2,4-DAPG)-producing fluorescent Pseudomonas spp. during wheat monoculture. The antibiotic 2,4-DAPG has a broad spectrum of activity and is especially active against the take-all pathogen. Based on genotype analysis by repetitive sequence-based-PCR analysis and restriction fragment length polymorphism of phlD, a key 2,4-DAPG biosynthesis gene, at least 22 genotypes of 2,4-DAPG producing fluorescent Pseudomonas spp. have been described worldwide. In this review, we provide an overview of G. graminis var. tritici, the take-all disease, Pseudomonas biocontrol agents, and mechanism of disease suppression.

질병의 중증도 판정지표로서 효과적인 임상병리검사 선정에 관한 연구

곽연식,최영수 한국의료QA학회 1998 가을학술대회 Vol.1998 No.-

질병의 중증도를 객관적으로 판정할 수 있는 임상병리 검사를 선정하기 위하여 조사한 결과, PT, pCO2, pH, pO2, 10단위 이상 적혈구체제 수혈, 혈청 CK-MB, Creat, CSF RBC 및 WBC, 혈액배양, K, Na 및 Alb 등의 양성 소견이 질병이 중증임을 시사하는 검사지표로 판정되었다.

의료정보학의 발전

郭然植 대한의학협회 1995 대한의학협회지 Vol.38 No.9

한국에서의 간호정보학 교육 방향 : 보건의료 정보학 교육의 필요성 및 경향

곽연식 서울대학교 간호과학연구소 2001 비정기 세미나 간행물 Vol.2001 No.1

남자 중,고등학생의 외모만족도에 따른 외모관리행동 및 인식에 대한 연구

곽연식,김미숙 한국의류학회 2009 한국의류학회 학술대회논문집 Vol.2009 No.1