마비성패류독의 생물학적 제독 -2. 해수에서 분리한 Enterobacter sp. CW-6를 이용한 마비성패류독의 분해-

박미정,이희정,이태식,김지회,이태호,PARK Mi Jung,LEE Hee Jung,LEE Tae Seek,KIM Ji Hoe,LEE Tae Ho 한국수산과학회 2000 한국수산과학회지 Vol.33 No.6

해수에서 분리한 마비성패류독 분해 균주 Enterobactersp. CW-6 균주의 마비성 패류독소 최적 분해 온도 및 시험 균주의 독소 분해과정 중의 독소 함량 및 구성성분을 조사한 결과는 다음과 같다. 시험 균주는 배양 적온으로 확인된 $30, 35^{\circ}C$에서 배양 5일만에 각각 최초 첨가 독력의 $56.8, 44.1{\%}$, 12일 후에는 $93.0, 79.5{\%}$를 분해하였다. 그러나, 배양 적온에서 벗어날수록 균주의 독소 분해 활성은 감소하여 $20^{\circ}C$에서는 배양 12일 후에도 최초 독력의 $69.0{\%}$가 잔존하였다. 시험 균주 Enterobacter sp. CW-6는 $30^{\circ}C$에서의 조독소를 이용한 분해 활성 측정에서 초기 농도 38.2nmole/g의 마비성패류독을 배양 8일 및 12일째 각각 $88.4, 92.7{\%}$ 분해하였다. 정제 독소를 이용한 분해 시험에서, 조독소 분해과정 중에 일시적으로 증가하는 STX group은 GTX2, 3에서 전환된 것으로 확인되었다. 시험 균주 Enterobactersp. CW-6는 정제 독소에 대해서도 강한 분해 활성을 나타내었으며, 최초 농도 47 nmole/g 의 GTX1과 37 nmole/g GTX4를 분해 과정 12일 후에 각각 $100, 90.8{\%}$ 분해하였으며, GTX2, 3 혼합물에 대하여는 최초농도 25.6 nmole/g의 독소를 12일 후에 $66.4{\%}$까지 분해하였다. Optimum temperature for paralytic shellfish poison (PSP) detoxofication of Enterobacter sp. CW-6 isolated from sea water and changes of contents and ingredients composition of PSP during bacterial detoxification process were investigated. Enterobacter sp. CW-6 detoxicated $61.5{\~}67.7{\%}\;and\;87.4{\~}96.8{\%}$ of initial PSP toxicity ($25.0{\~}28.5\;nmole/g$) after $5{\~}12$ days at 30 and $35^{\circ}C$, identified as optimal growth temperature, respectively. The detoxification rate of Enterobacter sp. CW-6 for crude PSP with initial concentration of 38.2 nmole/g after 8 and 12 days at $30^{\circ}C$ in the Marine broth was 88.4 and $92.7{\%}$, respectively. During bacterial detoxification process using crude toxin solution, temporary increasement of STX group was detected and identified that was derived from GTX2, 3 group. The detoxification rate of Enterobaoter sp. CW-6 on purified GTX1 and 4 with initial concentration 47 nmole/g and 37 nmole/g were more than $90{\%}$ after 12 days in the marine broth at $30^{\circ}C$. Enterobacter sp. CW-6 also showed a detoxification activity on purified GTX2 and 3, and the detoxification rate for the initial concentration 25.6 nmole/g after 12 days was $66.4{\%}$.

단삼(Salvia miltiorrhiza Bunge)으로 부터 Tanshinone IIA의 분리 및 함량분석

박미정,이승호,박정일,강신정,장승엽,이경순,손건호,Park, Mi-Jung,Lee, Seung-Ho,Park, Jeong-Hill,Kang, Shin-Jung,Chang, Seung-Yeup,Lee, Kyong-Soon,Son, Kun-Ho 한국생약학회 1999 생약학회지 Vol.30 No.2

The rhizomes of Salvia miltiorrhiza Bunge(Labiatae) has been used in Chinese traditional medicine for the treatment of coronary heart diseases and myocardial infarction. As a part of a research for standardization of crude oriental drugs, we have determined the content of tanshinone IIA in Salvia miltiorrhiza purchased from various regions of Korea. The HPLC method by which quantitative analysis was conducted, showed reproducible results and chromatographic isolation of tanshinone IIA was accomplished successively.

과민성 장 증후군의 특성과 병태생리 및 위험요인에 대한 고찰

박미정,최명애,김금순,홍해숙,이경숙,정재심,채영란,안경주,신기수,최정안,Park, Mi-Jung,Choe, Myung-Ae,Kim, Keum-Soon,Hong, Hae-Sook,Lee, Kyung-Sook,Jeong, Jae-Sim,Chae, Young-Ran,An, Gyeong-Ju,Shin, Ki-Soo,Choi, Jung-An 한국재활간호학회 2005 재활간호학회지 Vol.8 No.2

Purpose: Irritable bowel syndrome (IBS) is frequently yet little understood disease. Review was performed to promote understanding on the characteristics, pathophysiology, and risk factors of IBS. Content: IBS is characterized by abdominal discomfort associated with pain and altered bowel function; structural and biochemical abnormalities are absent. Generally IBS is more prevalent in women and people with higher educational and social background, but there are some controversies. IBS is diagnosed by the Rome II or Manning criteria after excluding organic gastrointestinal diseases. The pathophysioloy is explained by abnormal control mechanism of central and enteric nervous system. Mucosal immunity, secretions, and neurotransmitter are also associated with the hypersensitivity and motility change of bowel function. Stress is known as a major triggering factor and contributed to symptoms. Other risk factors are genetic elements, childhood experiences, inflammation, anxiety, depression, diet, and sleep disorders.

마비성패류독의 생물학적 제독 -1. 마비성패류독 분해세균의 분리 및 세균학적 특성-

박미정,이희정,이태식,박정흠,장동석,PARK Mi Jung,LEE Hee Jung,LEE Tae Seek,PARK Jeong Heum,JANG Dong Suck 한국수산과학회 2000 한국수산과학회지 Vol.33 No.6

For the establishment of biodetoxification method which can be acceptable for live bivalves, paralytic shellfish poison (PSP) detoxification bacteria were isolated from sea water and bivalves, and PSP detoxification activity and optimal growth condition of the isolated strains were investigated. from the bivalve and sea water samples, 8 strains of PSP detoxification bacteria were isolated. Of the isolated strains, CW-6 isolated from sea water shown strong PSP detoxification activity and decomposed completely 18 nmole/g of GTX2 after 3 days incubation in artificial medium. The selected stain CW-6 shown typical characteristics of the Enterobacter sp. and identified as Enterobacter sp, CW-6. Optimal growth condition of the Enterobacter sp. CW-6 were $35^{\circ}C$, pH 7 and $NaCl 1{\%}$, respectively. PSP 분해 활성 세균을 이용한 마비성패류독의 생물학적 제독 방법을 확립하기 위하여 진주담치 및 해수에서 마비성패류독 분해능이 있는 균주를 분리하였으며, 분리 균주의 독소 분해 활성 및 세균학적 특성 시험을 실시한 결과는 다음과 같다. 남해안 패류양식장에서 채취한 진주담치 및 해수 중에서 마비성패류독 분해능이 있는 8균주를 분리하였으며, 분리균주 중 $GTX 1{\~}4$ 혼합물 전반에 대하여 폭넓은 분해 활성을 나타낸 CW-6를 시험 균주로 선발하였다. 선발 균주 CW-6는 배양 3일만에 18nmole/g의 GTX2를 완전히 분해하는 강한 독소 분해 활성을 나타내었다. 선발 균주 CW-6는 생화학적 특성 시험 결과, Enterobacter속으로 동정되어 Enterobacter sp. CW-6로 명명하였으며, 이 균주는 $20{\~}40^{\circ}C,\;pH 7{\~}9$에서 왕성하게 증식하는 전형적인 중온세균의 특징과 염분농도 $6{\%}$에서도 최적 증식의 $46.8{\%}$ 정도 증식 가능한 내염성을 나타내었다.

치과병원 치주과 내원환자와 일반인 간의 구강위생 상태와 탐침시 출혈에 대한 통계학적 비교 연구

박미정,조규성,채중규,김종관,Park, Mi-Jeong,Cho, Kyoo-Sung,Chai, Jung-Kui,Kim, Chong-Kwan 대한치주과학회 1989 Journal of Periodontal & Implant Science Vol.19 No.1

박재삼 시에 나타나는 바다의 공간성 고찰

박미정(Park, Mi-Jung) 동남어문학회 2011 동남어문논집 Vol.1 No.32

This article will discuss ways to comprehend the poems of Park Jae Sam through sea space. Because the sea continuously affected in shaping his poems, to poet Park Jae Sam, space along with time becomes the premise. So to dwell on a space means to actively look at the inner consciousness that reflects a poet's consciousness and objective perception. Therefore, in relation to sea space in the poems of Park Jae Sam First it is circular repetitive reminiscence space. Second it is an altered space due to time. Third, it is self-aware embracing space. above 3 things are classified as such that will help understand his poem through the process of overcoming and accepting sorrow caused by sea space which either the poet consciously or unconsciously feels. To sum up, his poems on the sea is a lyric poetry where past co-exists with present, and is reminiscence, altercation and embracement space that mends fence with the real world.

Candida albicans에 대한 중성 전기분해수소수의 항진균 효과

박미정 ( Mi-jung Park ),최연희 ( Youn-hee Choi ),송근배 ( Keun-bae Song ),김은경 ( Eun-kyong Kim ) 대한예방치과·구강보건학회 2019 大韓口腔保健學會誌 Vol.43 No.4

Objectives: This study assessed the antifungal effects of neutral electrolysis hydrogen water, an agent known for its oral anti-bacterial effects on the growth of C. albicans in vitro. Methods: Hydrogen water, denture cleansing agent, and Listerine® were compared to a tap water control. We serially cultured C. albicans KCTC 27239 in SD media to analyze the inhibitory effect of each treatment on growth. We evaluated the data using repeated-measures ANOVA test and one-way ANOVA test, and a significant difference was defined as P<0.05. Results: Hydrogen water significantly inhibited the growth, but not the biofilm formation, of C. albicans compared to tap water control at 1-, 5-, and 10-minute time points (P<0.05). Conclusions: The growth of C. albicans was slower in hydrogen water than in tap water, thereby suggesting an inhibitory effect of hydrogen water. Hydrogen water may, therefore, be utilized as an antibacterial and preventive agent without any harmful effect on denture and oral tissues.

내면화된 수치심이 사회불안에 미치는 영향 : 자기비난의 매개효과

박미정(Mi-Jung PARK),박경(Kyung PARK) 한국상담심리교육복지학회 2020 상담심리교육복지 Vol.7 No.4

본 연구는 내면화된 수치심이 사회불안에 미치는 영향에서 자기비난의 매개효과를 알아보고자 하였다. 연구를 위해 서울 및 경기에 소재한 대학생 253명을 대상으로 자기보고식 설문지를 실시하였다. 연구 결과, 내면화된 수치심은 남녀 간에 유의미한 차이를 나타내고 있음을 검증하였다. 내면화된 수치심과 사회불안의 사회적 상호작용 불안 및 사회공포증, 내면화된 수치심과 자기비난, 사회적 상호작용 불안 및 사회공포증과 자기비난 등 모든 변인간의 정적인 상관관계를 확인하였다. 또한 내면화된 수치심이 사회적 상호작용 불안 및 사회공포증과의 관계에서 자기비난이 완전매개하고 있음을 검증하였다. 이는 내면화된 수치심이 사회불안에 직접적인 영향을 미칠 뿐 아니라, 자기비난을 통해 간접적으로 영향을 미치는 것을 의미한다. 사회불안의 사회적 상호작용 불안이 사회공포증 보다 자기비난의 매개효과에 대한 설명력이 더 높았다. 특정 상황 보다는 일상생활의 상호작용에서 자기비난을 통하여 더 높은 수준의 불안을 경험한다는 것을 설명하고 있다. 내면화된 수치심의 성격적인 특성을 가진 사람들이 모두 사회불안을 경험하는 것은 아니지만, 인지적인 전략으로 자기비난을 사용하게 됨으로써 사회불안에 유의미하게 영향을 미친다는 것을 알 수 있다. This study is aimed at identifying how the mediating effect of self-criticism in the effect of internalized shame on social phobia. For this, 253 university students based in Seoul and Gyeonggi Province were asked to complete self-reported questionnaire. The main results of this study are summarized as follows. First, all the variables addressed in this study were found to be static correlation. Second, the influence of internalized shame on self-criticism was significant, and self-criticism and internalized shame has a significant impact on social anxiety. There, internalized shame was proved to be mediated by self-criticism in relation to social anxiety. Third, as a result of examining the mediating effects of self-criticism on social interaction anxiety and social phobia, respectively, the ability to explain the mediating effects of self-criticism was higher than that of social phobia.

지속성 외래 복막투석 환자에서 영양상태와 투석의 적절도

박미정(Mi Jung Park),이균상(Kyun Sang Lee),조민석(Min Seok Cho),김정기(Jeong Ki Kim),박병석(Byoung Seok Park),마성권(Seong Kwon Ma),고정희(Jung Hee Ko),나명윤(Myong Yun Nah),염충호(chung Ho Yeum),정권(Jung Gwon),김수완(Soo Wan Kim) 대한신장학회 2001 Kidney Research and Clinical Practice Vol.20 No.4

Purpose - Malnutrition is common in CAPD patients and depends on many factors such as dialysis-related and nondialysis-related factors. The present study aimed to assess nutritional status, dialysis adequacy and their relationships with overall mortality and morbidity. Methods - We studied 102 patients who had been receiving CAPD for at least 6 months. Dialysis adequacy was assessed by parameters derived from urea kinetic modeling(UKM) and nutritional status was assessed by serum biochemical measurement, normalized protein catabolic rate(nPCR), normalized protein equvalent of total nitrogen appearance(nPNA) and urea kinetic studies. Spearman's simple correlation and multiple linear stepwise regression analysis were used to assess correlation between dialysis adequacy and nutritional status in CAPD patients. We compared the differences between patients who suffered morbid events, defined as either an infectious complication or hospitalization, and patients who remained well. Results : The results showed that the total dialysis dose(total weekly Kt/Vurea ) has statistically significant correlation with nPCR(r=0.234, p=0.028), nPNA (r=0.246, p=0.021), total weekly creatinine clearance (WCC)(r=0.479, p=0.0001), serum albumin levels(r= 0.233, p=0.029), serum cholesterol(r=0.266, p=0.013), serum BUN(r=-0.290, p=0.006) and serum creatinine levels(r=-0408, p-0.0001). nPNA was positively correlated with serum cholesterol(r=0.217, p=0.045), serum transferrin(r=0.218, p=0.042) and serum ferritin levels(r=0.220, p=0.043). Patients who suffered morbid events had an old age(p=0.001), long duration of CAPD(p=0.0001), higher CRP(p=0.021), lower serum albumin level(p=0.020), lower hematocrit(p=0.049) and lower WCC(p=0.017). Conclusions: These results indicate that adequate dialysis is very important for the maintenance of adequant nutrition because nutritional status positively correlated with dialysis dose, which is best assessed by UKM. In addition, assessment of nutritional status and dialysis adequacy are important in predicting clinical outcomes in CAPD patients.

만성 신부전 환자에서 고호모시스틴혈증에 관한 연구

박미정(Mi Jung Park),이균상(Kyun Sang Lee),조민석(Min Seok Cho),김정기(Jeong Ki Kim),박병석(Byoung Seok Park),마성권(Seong Kwon Ma),고정희(Jung Hee Ko),나명윤(Myong Yun Nah),염충호(chung Ho Yeum),김수완(Soo Wan Kim),김남호(Nam Ho Kim 대한신장학회 2001 Kidney Research and Clinical Practice Vol.20 No.4

Objective : It has been reported that hyperhomocysteinemia is an independent risk factor for atherosclerotic complications, although the mechanisms re- main unclear. The major determinents of total fasting plasma homocysteine(tHcy) concentrations have been recently reported but there are still conflicting data on the influence of those in peritoneal and hemodialysis patients. Therefore, we evaluated the prevalence and association of vascular complications and the determinents of hyperhomocysteinemia in chronic renal failure patients receiving conservative treatment(predialysis), peritoneal dialysis(PD) and hemodia-lysis (HD) patients. Methods We measured the factors, including fasting plasma vitamine levels(folate, vitamin E4 and vitamin B12), serum creatinine concentration, dialysis adequacy-related varibles as well as associated risk factors for vascular diseases that might affect tHcy concentrations in 37 predilysis, 30 PD, 34 HD patients and 21 normal persons. Continuous variables were compared using independent sample t-test. Spearman's correlation was used to determine the strength of association between tHcy and other predictive varia41es. Percentages were compared using Pearson's chi-square test or Fisher's exact test, depending on the frequencies. Independent determinents of tHcy concentration and atherosclerotic vascular complications were identified using multiple regression analysis. Results : The prevalence of hyperhomocysteinemia was 83.8, 72, 88% among predialysis, PD and HD patients, respectively(Odds ratio was 103.33, 81.43, 150.0 vs. controls, respectively). tHcy values in pre- dialysis, PD and HD patients are significantly higher than those in controls(24.68±9.01, 21.04±8.82, 23.62± 9.46 vs. 8.80±2.07 ㎛oL/L, repectively, p<0.01). Pre- dialysis, PD and HD patients with atherosclerotic vascular complications had higher tHcy concentrations than did predialysis, PD and HD patients with- out vascular complications(21.93±8.71 vs. 32.09±4.71 ㎛oL/L, p<0.01, 17.57±5.85 vs. 28.74±9.70 ㎛oL/L, p<0.01, 19.00±4.29 vs. 33.28±10.13, p<0.01 respectively). We also observed increasing odds ratios of vascular events with increasing tHcy concentrations. For predialysis, PD and HD patients, fasting plasma folate level had negative correlation with tHcy concentrations by spearman's simple correlaltion. And using muliple regression analysis, we recognized hyperhomocysteinemia is an independent risk factor for atherosclerosis and fasting plasma folate is a major determinent of tHcy concentrations in predialysis, PD and HD patients. Conclusions: Hyperhomocysteinemia in predialysis, PD and HD patients was more prevalent than that in norrnal controls. Risk of atherosclerotic vascular complications increased with increasing tHcy concentrations. Hyperhomocysteinemia is an independent risk factor for atherosclerosis and fasting plasma folate is a major determinent of tHcy concentrations in predialysis, PD and HD patients.