Ultrastructural studies of vitellogenesis in oocytes and follicle cells during oogenesis in female Protothaca (Notochione) jedoensis (Bivalvia: Veneridae)

강희웅,전제천,이기영,박관하,최기호 한국통합생물학회 2010 Animal cells and systems Vol.14 No.4

Ultrastructural studies of vitellogenesis in oocytes and follicle cells during oogenesis in female Protothaca (Notochione) jedoensis were investigated by histological and transmission electron microscope observations. In early vitellogenic oocytes, combined activities of the Golgi complex, mitochondria and rough endoplasmic reticulum in the cytoplasm are associated with autosynthetic vitellogenesis. Furthermore, at this time, many coated vesicles at the basal region of the oolemma of the oocyte lead to the formation of vesicles through endocytosis in the cytoplasm. Through the formation of the coated pits on oolemma during vitellogenesis, the uptake of extrafollicular precursors (nutritive materials) occurs in coated vesicles by endocytosis. Therefore, it is assumed that these exogenous materials are involved in heterosynthetic vitellogenesis. During late oogenesis, exogenous yolk precursors (yolk granules), lipid droplets and proteinaceous yolk granules are present in the cytoplasm of late vitellogenic oocytes. In mature oocytes,small yolk granules appear intermingled and form large mature yolk granules. Thus, two processes of vitellogenesis occur in oocytes by way of endogenous autosynthesis and exogenous heterosynthesis. The follicle cells attached to the oocytes appear to play an integral role in vitellogenesis in this study.

양식산과 자연산 황복, Takifugu obscurus(Abe)의 식품학적 품질평가

강희웅,심길보,강덕영,조기채,송기철,이진호,송흥인,손상규,조영제 한국양식학회 2007 韓國養殖學會誌 Vol.20 No.3

Gonadal Development, Spawning and Plasma Sex Steroid Levels of the Indoor Cultured Grunt, Hapalogenys nitens

강희웅,조재관,손맹현,박종연,홍창기,Jae Seung Chung,정애영 한국발생생물학회 2015 발생과 생식 Vol.19 No.1

The gonadosomatic index (GSI), gonadal development and changes in hormones in plasma level of the indoor cultured grunt (Hapalogenys nitens) were investigated by histological study from August 2011 to October 2012. The GSI showed similar trends with gonad developmental stages during the culture periods. Changes in plasma level of estradiol-17β of female H. nitens reached the highest value before the spawning period, and seasonal changes in plasma level of estradiol-17β were similar in trends of oocyte developments and GSI changes. Testosterone levels of male H. nitens reached the highest value before and after the spent stage. Ovarian developmental stages of H. nitens could be classified into early growing stage, late growing stage, mature stage, ripe and spawning stage, recovery and resting stage. The testicular developmental stages could be divided into growing stage, mature stage, ripe and spent stage, and recovery and resting stage.

Ultrastructural studies of spermatogenesis and the functions of Leydig cells and Sertoli cells associated with spermatogenesis in Larimichthys polyactis (Teleostei, Perciformes, Sciaenidae)

강희웅,정의영,정재승,이기영 한국통합생물학회 2013 Animal cells and systems Vol.17 No.4

Ultrastructural studies of spermatogenesis and the functions of Leydig cells and Sertoli cells were investigated by electron microscope observation. Ultrastructural characteristics of spermatozoa of Larimichthys polyactis (Sciaenidae) have a round head, a nucleus containing highly compact filamentous clusters of chromatin, slightly eccentric nuclear fossa, no acrosome, a short midpiece with a short cytoplasmic channel, and a flagellum showing the classic axoneme structure (92) and short irregular flagellum lateral fins. The spermatozoon of this species (Sciaenidae) is Perciformes type II: the flagellar axis is parallel to the nucleus, the centriolar complex is outside the nuclear fossa, the proximal centriole is anterior and perpendicular to the distal centriole, and no more than five spherical mitochondria are observed. Each of the well-developed Leydig cells observed during the periods of active meiotic division and before spermiation contains an ovoid vesicular nucleus; a number of smooth endoplasmic reticulum, tubular, or vesicular mitochondrial cristae; and several lipid droplets within the cytoplasm. This indicates that Leydig cells, as typical steroidogenic cells showing several cytoplasmic characteristics, are involved in male steroidogenesis. However, no clear evidence of steroidogenesis or of any transfer of nutrients from the cells to spermatids that appears to be associated with phagocytic function in the seminiferous lobule was observed for Sertoli cells. These findings indicate that Sertoli cells of this species function in phagocytosis and the resorption of products originating from degenerating spermatids and spermatozoa following spermiation.

서식지와 양식방법에 따른 큰가리비 (Patinopecten yessoensis) 성패의 성장 및 형태적 특징

강희웅,최진,남명모,김광일,임현정 한국패류학회 2017 The Korean Journal of Malacology Vol.33 No.4

The study investigated the growth and appearance characteristics of domestic Yesso scallops, Patinopecten yessoensis (Korea: Gangneung hanging culture, Pohang sowing culture) and imported Yesso scallops (Japan: Hokkaido sowing culture) which being sold in the eastern coast area of South Korea, and compared their commercial value. In domestic Yesso scallops reared by hanging method, the relative growth relationship for the shell height was shown as SL = 0.6658 SH + 35.388 (R2 = 0.7071), SW = 0.1896 SH + 7.52 (R2 = 0.2263), TW = 4.35 SH . 302.72 (R2 = 0.7007), MW = 1.9975 SH . 175.58 (R2 = 0.7523) in shell length, shell width, total weight, meat weight, respectively. Relative growth relationship of each regions in Yesso scallop was shown the straight on growth in all three different habitat. The percentage of shell height to shell length (SH/SL, %) was 103.95 ± 0.36% and the percentage of meat weight to total weight (MW/TW, %) was 41.91 ± 0.27%, they were shown significantly higher than others in domestic Yesso scallop reared by hanging method. In the sex ratio of Yesso scallops according to culture mode, the percentage of female was very low as 3.3% compared to 96.7% of male in one reared by hanging method in Korea. On the other hand, the percentage of female was similar with female as 41.1-44.4% in scallops reared by sowing method in Korea and Japan. The size of Yesso scallops was larger and the ratio of meat weight to total weight was higher in one reared by hanging method in Korea than others. Therefore, Yesso scallops reared by hanging method in Korea has a higher commercial value than Yesso scallops reared by sowing method in Japan.

자연산과 양식산 참조기의 식품학적 품질평가

강희웅,조영제,조기채,김종화,강덕영,박광재,심길보 한국수산과학회 2010 한국수산과학회지 Vol.43 No.1

The biochemical composition of wild and cultured yellow croaker, Larimichthys polyactis, was analyzed in this study. The moisture contents in wild and cultured yellow croaker was high: 75.2±1.60% and 79.5±1.95%, respectively. The crude lipid contents of wild and cultured yellow croaker were low; moreover, the crude protein and ash contents did not differ significantly(P>0.05). The total amino acid content of wild and cultured yellow croaker did not differ significantly; however, the cystine content of wild yellow croaker was higher than that of cultured yellow croaker. The essential /nonessential amino acid (E/NE) ratio in wild and cultured yellow croaker was 0.76±0.01 and 0.77±0.02, respectively. The free amino acid and extractive nitrogen contents of cultured yellow croaker were high and differed significantly. The water soluble vitamin(B1, B2, B6, B12, C and folate) and fat-soluble vitamin(A and E) contents did not differ significantly, expect for niacin. The niacin content of cultured yellow croaker was higher than that of wild yellow croaker. The fatty acid composition of wild and cultured yellow croaker did not differ significantly. The sodium, magnesium, and copper contents in wild yellow croaker were relatively low. In comparison, the calcium, phosphorus and iron contents in cultured yellow croaker were relatively high. Overall, the biochemical composition of wild and cultured yellow croaker did not differ significantly.

특집마당 : 유정용 강관의 기술개발 동향 (ERW 조관기술)

강희웅 대한금속재료학회 2012 재료마당 Vol.25 No.6

Jigsaw II 협동학습이 중학교 기술·가정 교과 '전기·전자 기술'단원에서 학업 성취도에 미치는 효과

강희웅,김진수 한국기술교육학회 2006 한국기술교육학회지 Vol.6 No.3

춘계학술대회 요약 : 구두발표 ; 6. 쥐노래미 (Hexagrammos otakii)의 생식생태

강희웅,정의영 한국어류학회 2003 한국어류학회지 Vol.15 No.2

서해권역 패류 생산의 장기 (1970-2022년) 변화 및 생산성 향상에 관한 고찰

강희웅,박광재,이경미,김정현 한국패류학회 2024 The Korean Journal of Malacology Vol.40 No.1

This study aims to explore measures to continuously improve shellfish productivity in the West Sea coastal region (Incheon metropolitan city, Gyeonggi-do, Chungcheongnam-do, and Jeollabuk-do), which has a high proportion of aquaculture among domestic shellfish production areas. To analyze changes in shellfish production in the West Sea coastal region over 52 years, we compared changes in tidal flat area and production by fishery and species from 1970 to 2022 using data from the KOrean Statistical Information Service (KOSIS, 2023). As a result of the analysis, West Sea region over the past 10 years (2013-2022) was a total of 50,092 tons (28,997 tons of offshore fisheries, 21,095 tons of shallow-sea aquacultures), aquaculture ratio accounted for 40.4%. Shellfish production by fishery in the West Sea area for 52 years (1970-2022) includes 23 species of offshore fisheries, and the main species targeted for fishing include sunray surf clams, surf clam, oysters, and manila clam. There are 16 species of shallow-sea aquacultures, and the main species targeted for aquaculture include manila clam, oysters, surf clam, and venus clams. Changes in tidal flat area due to reclamation projects had a significant impact on the production of burrowing shellfish. Over the past 31 years (1987-2018), the change in the area of the West Coast tidal flat decreased 590 km2 from 1,935.4 km2 to 1,345.4 km2, showing a decrease rate of 30.5%, a high decrease compares to other regions across the country. In conclusion, it is believed that the way to continuously improve the productivity of shellfish is to protect natural seed and shell growth areas, selecting habitat suitability and suitable sites for each species, managing seed release, and expanding shellfish seed production and intermediate cultivation infrastructure.