Technical Sessions - Session 13 : SPC 5 / Smooth Nonparametric Estimation of Mean Residual Life

(Myoung Hwan Na),(Sung Hyun Park),(Jae Joo Kim) 한국품질경영학회 1998 The 12th Asia Quality Management Symposium : Total Vol.- No.-

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Technical Sessions - Session 6 : Reliability / A Family of Tests for Trend Change in Mean Residual Life with Unknown Turning Point

(Myoung Hwan Na),(Jae Joo Kim) 한국품질경영학회 1998 The 12th Asia Quality Management Symposium : Total Vol.- No.-

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평균잔여수명의 경향 변화에 대한 검정에 관한 연구

나명환(Myoung Hwan Na),이현우(Hyun Woo Lee),김재주(Jae Joo Kim) 한국품질경영학회 1998 품질경영학회지 Vol.26 No.4

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Evaluation of the Efficacy of Kochiae fructus Extract in the Alleviation of Carbon Tetrachloride-induced Hepatotoxicity in Rats

Na-Young Kim,Jeong-Sook Lee,Seong-Ji Kim,Myoung-Ju Park,Seok-Hwan Kim 한국영양학회 2005 Nutritional Sciences Vol.8 No.4

Evaluation of the Efficacy of Kochiae fructus Extract in the Alleviation of Carbon Tetrachloride-induced Hepatotoxicity in Rats

Kim Na-Young,Lee Jeong-Sook,Kim Seog-Ji,Park Myoung-Ju,Kim Seok-Hwan The Korean Nutrition Society 2005 Nutritional Sciences Vol.8 No.4

Hepatoprotective effects of the extract of Kochiae fructus (KF), a traditional oriental medicinal plant, were evaluated against carbon tetrachloride($CCl_4$)-induced liver damage in rats. Male Sprague-Dawley rats were divided into control, $CCl_4,\;CCl_4$ plus methanol extract of KF (KFM-$CCl_4$), and $CCl_4$ plus butanol extract of KF (KFB-$CCl_4$) groups. KFM and KFB were orally administered once a day (200 mg/kg body weight) for 14 days. A mixture of 0.2 mL/100 g body weight of $CCl_4$ in olive oil was injected at 30 minutes after the final administration of KFM and KFB. The KFB pretreatment resulted in a significant decrease in the serum transaminase and lactic dehydrogenase levels in the $CCl_4$-treated rats. The $CCl_4$ treatment significantly lowered the activities of glutathione, glutathione reductase (GR), glutathione-S-transferase (GST), superoxide dismutase (SOD), catalase and glutathione peroxidase (GSH-Px). However, pretreatment with KFM and KFB resulted in a significant increase in the glutathione, GR and GST levels. KFB increased the activities of SOD, catalase and GSH-Px, but KFM did not alter them. Pretreatment with KFM and KFB resulted in a significant decrease in the production of aminopyrine N-demethylase in the $CCl_4$-treated rats. KF extract would appear to contribute to alleviate the adveISe effect of $CCl_4$ treatment by enhancing the hepatic antioxidant defense system.

Occurrence of Pasteuria spp. Associated with Plant-parasitic Nematodes in Korea

조명래,Myoung Soon Yiem,Heung Yong Jeon,Hye Rim Han,Hyung Hwan Kim,Seung Yong Na,Chun Keun Lim 한국응용곤충학회 2005 Journal of Asia-Pacific Entomology Vol.8 No.2

A survey was conducted on the occurrence of Pasteuria spp. associated with plant-parasitic nematodes in greenhouse and open field soils in Korea from 1996 to 2000. Among the 372 soil samples assayed, 20 samples had plant-parasitic nematodes attached with Pasteuria spp. endospores. The nematodes found with Pasteuria endospores and their collection sites were Meloidogyne spp. in Sungju, Cheju, and Yeoju and Helicotylenchus sp., Pratylenchus sp., Heterodera sp., and Aphelenchus sp. in Cheju. Morphological characteristics of the endospores on Meloidogyne incognita juveniles observed under light, scanning and transmission electron microscopes were same as the previous description of Pasteuria penetrans Sayre & Starr 1988.

지부자 활성성분이 D - Galactosamine 투여에 의한 흰쥐의 간손상에 미치는 영향

김나영(Na-Young Kim),이정숙(Jeong-Sook Lee),박명주(Myoung-Ju Park),이경희(Kyung-Hee Lee),김석환(Seok-Hwan Kim),최종원(Jong-Won Choi),박희준(Hee-Juhn Park) 한국식품영양과학회 2004 한국식품영양과학회지 Vol.33 No.8

지부자(Kochiae Fructus)의 생리활성물질 검색 및 간손상에 미치는 영향을 연구할 목적으로 실험동물에 지부자 추출물을 경구투여한 후 GaIN으로 간손상을 유발하여 혈액 및 간조직의 생화학적 변화를 관찰하고 free radical의 생성계와 해독계의 활성에 미치는 영향을 검토한 결과는 다음과 같다. GaIN 단독투여군은 대조군에 비하여 AST와 ALT가 증가하였으나, KFB, oleanolic acid, momordin Ic 투여군에서는 GaIN 단독투여군에 비해 유의적 감소를 보였다. 간조직의 지질과산화 함량은 GaIN 단독투여군이 대조군과 비교하여 증가하였고, KFM 200-GaIN군, KFB 200-GaIN군, momordin Ic 30-GaIN군과 oleanolic acid 30-GaIN군은 대조군에는 미치지 못하였으나 GaIN 단독투여군에 비해 현저히 감소하였다. XO, AO의 활성은 대조군보다 GaIN 단독투여군에서 유의적으로 증가하였으며, KFB 200-GaIN군, momordin Ic 30-GaIN군과 oleanolic acid 30-GaIN군의 XO와 AO의 활성은 GaIN 단독투여군보다 낮게 나타났다. 간조직 중의 GSH 농도는 GaIN 단독투여군이 대조군에 비해 현저히 감소하였고, KFB 200-GaIN군과 oleanolic acid 30-GaIN 군은 GaIN 단독투여군과 비교시 증가를 보였고, momordin Ic 30-GaIN군은 대조군에 가깝게 회복되었다. GaIN 단독투여군의 γ-GCS와 GR의 활성은 대조군에 비하여 유의한 감소를 보였고, momordin Ic 30-GaIN군의 γ-GCS의 활성은 대조군에는 미치지 못하였지만 GaIN 단독투여군에 비해 유의하게 개선되었다. KFM 200-GaIN군, KFB 200-GaIN군, momordin Ic 30-GaIN군과 oleanolic acid 30-GaIN군의 GR활성은 GaIN 단독투여군보다 유의한 증가를 보였다. GST 활성은 GaIN 단독투여군이 대조군에 비하여 현저한 감소를 나타내었고, KFM 200-GaIN군, KFB 200-GaIN군, momordin Ic 30-GaIN군과 oleanolic acid 30-GaIN군은 대조군 수준에는 못미쳤으나 GaIN 단독투여군보다 통계적으로 유의한 증가를 관찰할 수 있었다. SOD, catalase 및 GSH-Px의 활성은 대조군에 비하여 GaIN 단독투여군에서 감소를 보였고, SOD와 catalase 활성은 KFM, KFB와 oleanolic acid의 투여로 GaIN 단독투여군보다 높게 나타났다. 특히 momordin Ic 30-GaIN군의 SOD 활성은 대조군에 가깝게 개선되었다. GSH-Px의 활성은 KFM 200-GaIN군, KFB 200-GaIN군과 oleanolic acid 30-GaIN군은 대조군 수준에는 미치지 못하였으나 GaIN 단독투여군에 비해 현저히 증가하였고, 특히 momordin Ic 30-GaIN군은 대조군에 가깝게 증가되었다. 이상의 결과를 종합하여 볼 때 지부자로부터 분리한 momordin Ic가 GSH 농도를 증가시키고 활성산소 해독계에 관여하는 효소의 활성을 증가시킴으로서 GaIN으로 인한 간손상을 완화시키는 것으로 사료되어진다. This study was conducted to investigate the biological activity and hepatoprotective effect of various fractions and isolated compounds from Kochiae fructus (KF) extract on D-galactosamine (GaIN)-intoxicated rats. Male Sprague-Dawley rats were divided into control, GaIN treated group (GaIN), GaIN plus KF methanol extract treated group (KFM 200-GaIN), GaIN plus KF butanol extract treated group (KFB 200-GaIN), GaIN plus momordin Ic treated group (Momordin Ic 30-GaIN) and GaIN plus oleanolic acid treated group (Oleanolic acid 30-GaIN). KFM (200 ㎎/㎏ BW), KFB (200 ㎎/㎏ BW), momordin Ic (30 ㎎/㎏ BW) and oleanolic acid (30 ㎎/㎏ BW) were orally administered once a day for 14 days. GaIN (400 ㎎/㎏ BW) was injected at 30 minutes after the final administration of the compounds. The activities of serum aspartate aminotransferase and alanine aminotransferase were increased in the GaIN group compared to the control group and significantly lower in the KFB 200-GaIN, momordin Ic 30-GaIN and oleanolic acid 30-GaIN group than in the GaIN group. Hepatic lipid peroxide level was increased in the GaIN group compared to the control group and was lower in the KFM 200-GaIN, KFB 200-GaIN, momordin Ic 30-GaIN and oleanolic acid 30-GaIN group than in the GaIN group. Activities of xanthine oxidase and aldehyde oxidase in liver were higher in the GaIN group than in the control group and were significantly decreased in the KFB 200-GaIN, momordin Ic 30-GaIN and oleanolic acid 30-GaIN group compared to the GaIN group. Hepatic glutathione, γ-glutamylcysteine synthetase and catalase activities were decreased in the GaIN group compared to the control group and were higher in the KFB 200-GaIN, momordin Ic 30-GaIN and oleanolic acid 30-GaIN group than in the GaIN group. Activities of hepatic glutathione reductase, glutathione S-transferase, superoxide dismutase and glutathione peroxidase were lower in the GaIN group than in the control group and were improved in the KFM 200-GaIN, KFB 200-GaIN, momordin Ic 30-GaIN and oleanolic acid 30-GaIN group compared to the GaIN group. Therefore, the current results indicate that momordin Ic administration alleviated the GaIN-induced adverse effect through enhancing the antioxidant enzyme activities.

말굽버섯 추출물이 Streptozotocin으로 유발된 당뇨 흰쥐의 혈당, 지질대사 및 면역세포에 미치는 영향

김나영(Na-Young Kim),정호금(Ho-Kum Jung),박명주(Myoung-Ju Park),김석지(Seog-Ji Kim),김석환(Seok-Hwan Kim),최종원(Jong-Won Choi),이정숙(Jeong-Sook Lee) 한국식품영양과학회 2005 한국식품영양과학회지 Vol.34 No.6

말굽버섯(Fomes fomentarius)이 당뇨에 미치는 영향을 연구할 목적으로 실험동물에 STZ를 투여하여 당뇨를 유발시킨 후 말굽버섯 물추출물과 메탄올 추출물을 2주간 투여하여 혈당, 지질함량 및 면역에 미치는 영향을 검토한 결과는 다음과 같다. 혈당은 당뇨로 인해 증가하였으나 말굽버섯 추출물 투여군에서 유의적으로 감소를 보였다. 혈청과 간의 총콜레스테롤과 중성지방 함량은 말굽버섯 추출물 급여로 DM군에 비하여 현저한 감소를 나타내었다. HDL-콜레스테롤 함량과 GSH-Px의 활성은 말굽버섯 추출물 급여 시에는 DM군과 비교하여 유의한 증가가 나타났다. 혈청 보체 C3의 함량은 대조군에 비하여 DM군에서 유의한 감소를 보였고, DM-FM 200군에서 대조군 수준으로 회복이 관찰되었다. B cell과 T cell은 DM군에서 유의한 감소를 보였고, 말굽버섯 추출물 투여로 유의적으로 증가되었다. Helper T cell과 suppressor T cell의 분포는 DM군에서 유의하게 감소되었으나, 말굽버섯 추출물 투여로 유의한 증가를 나타내었다. 이상의 결과를 종합하여 볼 때 말굽버섯의 물 추출물과 메탄올 추출물은 혈당조절 기능, 지질대사 개선 및 면역작용을 활성화시킴으로서 STZ의 투여로 인한 당뇨의 증상을 완화시키는 것으로 사료된다. This study was conducted to investigate the effect of extract of Fomes fomentarius (FF) on blood glucose, lipid profile, antioxidant enzymes and immune cells in streptozotocin (STZ)-induced diabetic rats. Male Sprague- Dawley rats were divided into control, STZ-induced diabetic group (DM), STZ plus FF water extract treated group (DM-FW 200) and STZ plus FF methanol extract treated group (DM-FM 200). FW (200 ㎎/㎏ BW) and FM (200 ㎎/㎏ BW) were orally administered once a day for 14 days. Admdinistering FW and FM to STZ-induced diabetic rats lowered the blood glucose level. The supplementation of FW and FM suppressed the increase in the total cholesterol and triglyceride levels in the serum and liver of the diabetic rats. The high density lipoprotein-cholesterol level and glutathione peroxidase activity were higher in the FF-supplemented group compared to the diabetic group. Administering FW and FM increased the suppress in the serum complement component C3, whole blood B-cell, T-cell, helper T cell and suppressor T cell of the diabetic rats. Therefore, it could be suggested that FW and FM are alleviated the diabetic complication through enhancing the hyperglycemia and preventing diabetic complications.

Occurrence of Pasteuria spp. Associated with Plant-parasitic Nematodes in Korea

Cho Myoung Rae,Yiem Myoung Soon,Jeon Heung Yong,Han Hye Rim,Kim Hyung Hwan,Na Seung Yong,Lim Chun Keun Korean Society of Applied Entomology 2005 Journal of Asia-Pacific Entomology Vol.8 No.2

A survey was conducted on the occurrence of Pasteuria spp. associated with plant-parasitic nematodes in greenhouse and open field soils in Korea from 1996 to 2000. Among the 372 soil samples assayed, 20 samples had plant-parasitic nematodes attached with Pasteuria spp. endospores. The nematodes found with Pasteuria endospores and their collection sites were Meloidogyne spp. in Sungju, Cheju, and Yeoju and Helicotylenchus sp., Pratylenchus sp., Heterodera sp., and Aphelenchus sp. in Cheju. Morphological characteristics of the endospores on Meloidogyne incognita juveniles observed under light, scanning and transmission electron microscopes were same as the previous description of Pasteuria penetrans Sayre & Starr 1988.

간성 뇌증의 치료로서 Rifaximin(Normix) 단기간 투여의 효능과 안전성

김명환,이관식,문병수,박인서,송건훈,전재윤,한광협,문영명,백용한,나호균,윤석화 대한간학회 2001 Clinical and Molecular Hepatology(대한간학회지) Vol.7 No.1

Background/Aims: A prospective, randomized study was performed to evaluate the efficacy and safety of the short-term administration of rifaximin in the treatment of hepatic encephalopathy. Methods: Of the 64 patients diagnosed as having decompensated liver cirrhosis with hepatic encephalopathy, 39 patients were randomized to receive rifaximin and 25 patients to receive lactulose for seven days. Before and after the treatment we assessed changes in the level of serum ammonia, flapping tremor, patient's mental status, number connection test (NCT), and hepatic encephalopathy indices. Results: In rifaximin-treated group, the mean grade of serum ammonia (1.8→0.9), mental status (1.3→0.3), NCT (3.0→2.0), and flapping tremor (1.7→0.4) were improved after treatment. In the lactulose-treated group, the mean grade of serum ammonia (1.9→1.0), mental status (1.5→0.5), NCT (3.3→2.1), and flapping tremor (1.4→0.3) were improved after treatment. Side effects of abdominal pain (rifaximin group) and excessive diarrheaose group) were noted in 2 cases. The efficacy of treatment was not significantly different between rifaximin and lactulose-treated groups (84.3% vs. 95.3%). Conclusion: Rifaximin was as efficient and safe in the treatment of hepatic encephalopathy as lactulose in terms of efficacy. Rifaximin may be useful drug for the short-term treatment of hepatic encephalopathy.(Korean J Hepatol 2001;7:55-60)