Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

韓國産 자작나무科의 系統分類學的 硏究(序論)

李相泰,蘇雄永,田寬培,李愚喆 全北大學校 生物學硏究所 1981 生物學硏究年報 Vol.2 No.-

The present paper is an introduction to the whole project of "The systematic studies on Korean Betulaceae". Three studies under the project were performed this year and presented in this report : a review of taxonomic literatures and the construction of distribution maps by Woo-Tchul Lee, pollen morphology by Sang-Tae Lee and Moo-Yeol Kim, and wood anatomy by Woong-Young Soh and Kwan-Bae Jeon.

자연산 점몰개, Squalidus multimaculatus Hosoya et Jeon의 새인두흡충, Clinostomum complanatum (Rudolphi, 1814) 감염

박명애 ( Myoung Ae Park ),서정수 ( Jung Soo Seo ),정승희 ( Sung Hee Jung ),최희정 ( Hee Jung Choi ),전은지 ( Eun Ji Jeon ),지보영 ( Bo Young Jee ),이완옥 ( Wan-ok Lee ),우승호 ( Sung Ho Woo ),이은혜 ( Eun Hye Lee ) 한국어병학회 2011 한국어병학회지 Vol.24 No.2

The metacercariae of Clinostomum complanatum (Rudolphi, 1814) were isolated in the whole body muscle of wild freshwater fish, spotted barbel gudgeon (Squalidus multimaculatus Hosoya et Jeon) from the local river in Yeongdeok, Gyeongsangbuk-do, Korea. The infected fish with bumpy body surface by yellow grub showed erratic swimming behavior and some fish were died. The isolated excysted metacercariae were tongue shaped and 2.85~5.89 mm × 0.8~1.99 mm in size. 1~98 metacercariae were isolated in individual fish and mean infection intensity was 25. All examined adult spotted barbel gudgeon sizing 7 cm in body length were infected. This is the first report of disease outbreak and mortality caused by C. complanatum infection in wild freshwater fish in Korea.

철근콘크리트 구조물의 균열발생 현황 및 원인 조사연구

전광우,정재동,최영화 대구대학교 과학기술연구소 1997 科學技術硏究 Vol.4 No.3

The purpose of this study is to propose the pertinent plan for crack through analyzing types, styles, periods, parts and features of crack for national concrete-related pursuers in domestic working site. And this is a basic study on control of crack which is presented to reinforced concrete structure. For these purposes, as we make check-list about each cause of crack and eliminate the cause of crack in first place in job site, we can make an effective method of control the crack as a whole.

Hough 변환에 의한 고속 원형물체 검출 알고리즘

전호민,최우영 명지대학교 산업기술연구소 2001 産業技術硏究所論文集 Vol.20 No.-

The several algorithms to detect circular boundaries from a digital image by Hough transform have been recognized as a robust technique. However, the algorithms are limited by slow speed and excessive memory. In this paper, a fast algorithm to detect the circular object with unknown radius is proposed. In this algorithm, the divide-and-conquer method is applied to reduce the computation time by using the property of area of cumulated points by the Hough transform. And the requirement of memory can be limited to the one 2-dimensional array of which size is the same as the source image. Experimental results show that the proposed algorithm gives better performance in comparision with Davies' Hough transform algorithm.

CT flouroscopy를 이용한 중재적 시술의 유용성

전혜정,박정희,서보경,박상우,신현준,최영철,한혜승,이창희 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.14 No.-

Conventional CT-guided needle localization has been reported many times, but a few reports of interventional procedures by using multidetector CT scan with CT-fluoroscopy have been described. We presented 36 cases of CT-fluoroscopy guided interventional procedures focusing on the puncture technique. Two cases of small pneumothorax and one case of pulmonary hemorrhage out of 2lcases after lung biopsy, were not needed any treatments We can easily and accurately access to the lesion by using CT-fluoroscopy and unique our marker system.

오존노출이 실험적으로 유발된 알레르기성 비염에 미치는 영향

전우진,변상내,김용민,김병국,나기상,박찬일 대한이비인후과학회 2000 대한이비인후과학회지 두경부외과학 Vol.43 No.11

남한 내 북한이탈주민들의 3년간 사회의식 변화 및 생활 만족도에 대한 추적연구

전우택,유시은,조영아,홍창형,엄진섭,서승원 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.3

Objectives : The aim of this study was to examine the change in attitudes and satisfaction level of North Korean defectors in South Korea over a 3 year period (2001-2004). Methods : In our first study in 2001, a total of 200 detectors were interviewed. In the follow up study after 3 years, 151 defectors were re-interviewed by an in-person interview. Results : The level of satisfaction to the governmental support policy to defectors decreased significantly. However, the general level of satisfaction increased slightly (from 3.52 to 3.56 ; 5-score scale). This shows that North Korean defectors are stably adapting to South Korean society. The level of satisfaction significantly correlated with the defectors' confusion of the different value systems, the defectors' understanding of the South Korean people, satisfaction with their physical and mental health, and the Prejudice of South Korean people to defectors. In terms of the attitudes, the type of schooling, the military service and the communist party membership in North Korea were not related to the change in attitude. However, there was a significant correlation with gender and age. Women showed a greater change in attitude compared to men. Older people had difficulty in understanding the South Korean people, but they showed little psychological affiliation to North Korea. Moreover, they showed a high level of satisfaction with the economic situation in South Korea and had an optimistic viewpoint for their future than younger people. Even though many defectors attended churches, religion was not associated with the level of satisfaction or their change in attitude. Conclusion : The factors that influence the level of satisfaction and attitudes of North Korean defectors have changed over 3 years. Therefore, the government, nongovernmental organizations (NGOs) associated with defectors and the churches need to be more sensitive to these changes and make plans to support the adaptation of defectors to South Korean society.

정상 인체피부섬유모세포종에 대한 벤조산의 독성효과

전주원,손영우,서부일,한두석 대한본초학회 2004 大韓本草學會誌 Vol.19 No.1

Objective : The aim of this study was to evaluate cytotoxic effect of benzoic acid on normal human skin fibroblast cell line , Detroit 551. Methods : The cytotoxicity was determined by colorimetric assays such as MTT assay or XTT assay. The microscopy was carried out to observe Detroit 551 cell line. Cells were incubated with various concentrations of benzoic acid in CQ incubator controlled with 37 c for 48 hours. Results : The cytotoxic effect of benzoic acid on Detroit 551 did not show a positive significance at 50 uM and 100 uM benzoic acid by MTT assay or X T T assay compared with control. MTTB and XTTg were 900.6 uM and 974.6 uM, respectively. In microscopic study, Detroit 551 treated with benzoic acid showed a slightly decreased cell number, while the morphological changes of cell was not showed. Conclusion : Above the results, the cytotoxicity of benzoic acid was midtoxicity on normal human skin fibroblasts cell line. Detrit 551

탈북자들의 신앙 경험과 교회의 통일 준비

전우택,조영아 연세대학교 통일연구원 2003 통일연구 Vol.7 No.2

This study was conducted to investigate the religious experiences of North Korean defectors in South Korea and the preparation of the Church for the Korean unification. Twenty-three North Korean defectors were interviewed qualitatively, and the data from the interview were analyzed using the theme analysis method. Results showed that the reasons why North Korean defectors held a religion in South Korea were as follows: (1) to receive help for overcoming life difficulties (2) to maintain relationship with the people who helped the defectors when they entered South Korea (3) to broaden personal connections (4) to receive financial support and (5) to be guided by moral aspects of the religion. On the other hand, North Korean defectors reported the reasons why they had difficulties in keeping religion and why they abandon ed it as follows: (1) unfamiliarity with religion (2) religion posing aspects that were similar to Ju-Che ideology, which were educated in North Korea (3) negative experiences in the church and (4) their absolute judgemental attitude. Theses results suggests ways that South Korean churches could prepare for Korean unification as follows: (1) Churches should try to understand the psychological and cognitive characteristics of North Korean people and North Korean defectors (2) need to develop religion education programs for North Korean defectors (3) need to confirm the basic doctrine of Christianity in preparing unification and lastly (4) need to be more active, balanced, and initiative i n the process of unification.