소배양법으로 배양한 생쥐췌장 베타세포의 형태학적 검정

유광성,전현우,박주홍,윤지원,고광삼 朝鮮大學校 附設 醫學硏究所 1986 The Medical Journal of Chosun University Vol.11 No.1

생쥐췌장 베타세포를 보다 순수하게 배양하기 위해 변형 고안한 소배양법을 검정하였다. 이 소배양법에 있어서는 96well plate에 islet cell을 이식하고 decantation한 다음 thimerosal에 노출시켜 많은 viable cell을 얻을 수 있었고, 생후 7일째의 생쥐의 췌장베타세포의 생존율이 가장 높았다. 또 이 소배양법으로 배양한 생쥐췌장 베타세포들은 위상차현미경, FITC-labelled anti-insulin antibody 염색 및 전자현미경으로 검정한 결과 형태학적으로 정상이었다. 기계적 선택방법으로서 생쥐췌장 베타세포의 decantation 최적시간은 세포를 이식한 후 12∼15시간 이었으며, 화학적 선택방법으로 0.6∼0.8㎍/㎖의 thimerosal에 노출시켜 fibroblastoid cell로 부터 생쥐췌장 베타세포의 분리를 증가시킬 수 있었으며, 두가지 방법을 동시에 응용하여 더 좋은 효율을 얻을 수 있었다. 또 이 소배양법에 있어서 cell duplication은 배양후 36시간후에 2배, 72시간후에 3배가 되었으며, 평균 generation시간은 46.8±7.0시간 이었다. 이들 결과로 미루어 보아 이소배양법은 생쥐췌장 베타세포 배양의 유용한 방법으로서, 췌장 베타세포에 연관된 각종 연구에 이용할 수 있을것으로 사료된다.

고등학생들의 에이즈(후천성 면역 결핍증)에 대한 지식과 태도에 관한 연구 : K시 일부 고등학생 대상

박인혜,윤현숙,한유정 韓國學校保建學會 1996 韓國學校保健學會誌 Vol.9 No.2

This descriptive study was done to investigate the degree of knowledge and attitudes, the need of health education, and the relationship between knowledge and attitudes concerning AIDS in senior high school students. The data was collected from 386 senior high school students(200 boys, 180 girls) in K city. The data was gathered by a self reporting questionnaire, from Nov. 22nd to the 30th, 1995. Data was analyzed using the descriptive statistics methods: t-test, F-test, Pearson-Correlation Coefficient, GLM with the statistical computer package, SAS. The Result of this study were as follows : 1. The mean score of knowledge about AIDS was 42.70 from a total score of 58, showing significant differences between boys and girls (p< 0.0001). 2. The mean score of attitudes about AIDS was 29.95 from a total score of 40, showing no significant differences between boys and girls. 3. The selected contents that the students want to learn were prevention, etiology, transmission, symptoms, and treatment of AIDS in that order. 4. The relationship between knowledge and attitude about AIDS show a positive correlation and is statistically significant(r=0.27, p< 0.0001). The information obtained from this study will provi a useful data to develop a practical health education program about AIDS for senior high school students.

1-Arylpyrrole로부터 9-Arylcarbazole의 합성

朴유미,金寅植,李龍均,成大東,金東賢,金倫瑛,鄭大一 東亞大學校附設基礎科學硏究所 1997 基礎科學硏究論文集 Vol.14 No.1

Carbazoles were discovered in anthracene oil of coal tar, and are the parent structure of a number of heterocyclic compounds. Much attention has been paid to carbazole derivatives recently as the materials for semiconductors and photoconductive compounds. Many conventional methods for synthesis of carbazoles were known such as Grabe-Ullmann methods and the Tauber methods. In the course of the investigation for the synthesis of pyrrole derivatives 3. We have found the formation of 9-arylcarbazoles 4 under refluxing glacial acetic acid. Thus we report the results here.

Propylthiouracil 투여에 의한 갑상선 기능저하 흰쥐 조직에서 알코올 탈수소효소 활성의 변화

백상현,김동선,이창범,박용수,안유헌,김태화,기춘석,강주섭 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.2

Background: Thyroid hormone has been known to affect hepatic alcohol dehydrogenase (ADH) activity. Although the liver is the principal site of ethanol metabolism, stomach is also responsible in part for ethanol oxidation. The effects of thyroid hormone on ADH activity in gastric mucosa and other tissues of rats had not been previously examined. Method: The effects of thyroid hormone on liver, stomach, lung, and kidney ADH activities (nM of NADH/min/mg of cytosolic protein) have been investigated in male Sprague Dawley rats treated with propylthiouracil (50 mg/kg) for 14 days. Results: Whereas hepatic ADH activities were not changed by treatment with PTU(42.9(8.6 vs 45.2 (10.1), gastric ADH activities in PTU-treated rats increased by 258.8% of control rat (6.3 ( 0.6 vs 2.2 ( 1.2, p〈0.001). In the activities of other tissues, PTU treatment decreased lung ADH activity by 59.7% of control, and increased kidney ADH activities by 247.1% of control rats. Conclusion: These data suggest that hypothyroidism causes an increase of gastric alcohol metabolism, and that the increase of gastric ADH activity can partly restore the first pass metabolism of ethanol in hypothyroid rats.

카올린에서 추출한 Silica로부터 탄소 환원질화에 의한 질화규소의 합성

신현택,형유업,박찬욱,배원태 慶尙大學校生産技術硏究所 1987 生産技術硏究所論文集 Vol.3 No.-

Nitriding behavior of the mixtures of amorphous silica extractracted from San Chung White kaolin and activated carbon Were studied by varying the carbon content, reaction temperature, and time under N₂atmosphere. Experimental results obtained from XRD, SEM and TEM indicated that the nitridation of the mixtures initiated below 1200℃ and mullite, silicon oxynitride, β-Sialon, β-SiC, and α-and β-Si₃N₄were identified as reaction products. Optimum nitridation condition of extracted silica were as follows. 1. Carbon/Extracted silica (in weight ratio)=3. 2. Reaction temperature; 1350℃ 3. Reaction time ; above 1hour.

남녀 중학생의 성에 대한 지식, 태도, 성교육요구도에 관한 연구 : -Junior high school St grade students in Kwang-Ju city- -광주 시내 일부 중학교 1학년 대상으로-

윤현숙,박인혜,한유정 韓國學校保健學會 1996 韓國學校保健學會誌 Vol.9 No.1

The purpose of the survey was to identify the degree and the relationship sexual knowledge, sexual attitude and need of sex education. This information will provide useful data for a more systematic desirable, and practical sex education. The data was collected from 300 first grade schoolchildren (150 boys and 150 girls) of two Junior high school in Kcity. The data was gathered by questionnaire from Nov.21 to 28, 1995. The questionnaire was modified by authors using the questionnaire developed by Lief & Reed. Data was analyzed by using the statistical computer package, SAS to mainpulate the data along with percentages, means, standard deviation, t-test, ??-test GLM, and Pearson correlation coeffiency. The results in this study were summarized as follows : 1. Sexual knowledge. - The mean score of sexual knowledges showed no significant differences between boys and girls(p<0.6180), but in the areas of biological differences(boys; 11.57±2.43, girls; 10.93±2.41, p<0.0242), and pregnancy physiology(boys; 9.28±1.87, girls; 10.04±2.42, p<0.0026) showed significant difference between boys and girls. 2. Sexual attitudes. - The mean score of sexual attitudes showed no significant differences between boys and girls(p<0.8286), but in the areas of masturbation(boys; 6.69±2.22, girls; 5.65±1.88, p<0.0001), and premarital intercourse (boys; 5.42±1.35, girls; 6.00±1.33, p<0.0002) showed significant difference between boys and girls. 3. Need of sex education. - The majority of the subjects wanted to learn about sexual delinquency and it's prevention(57.7%), form a friendship with the other sex(56.3%), physical differences of the other sex(52.0%), psychological differences and the characteristics of the other sex(50.3%) and meaning of love(50.3%). 4. The relationships between sexual knowledges and sexual attitudes. - Those who had higher sexual knowledges showed more positive attitudes towards sex, and showed statistical significance(r=0.2074, p<0.0003). 5. The relationships bwtween general characterisitics and sexual knowledge. - Better students(by self-perception of shool performance) had higher knowledge scores about sex. 6. The relationships bwtween general characterisitics and sexual attitudes. - Those who wanted to learn about sex, and better students had higher attitude scores about sex. - Those who were in high stadard of living had higher sttitude scores sex as well. Suggestions based on above study are as follows: 1. The planned sex education schowed be performed to the schoolchildren establish responsible attitudes about sex. 2. Systematic sex education through formal shool education according to age is recommended. 3. Further studies should be done to measure the effect of sex education based on the needs of the schoolchildren.

완전 방실차단을 동반한 급성 심근염

이유선,정진옥,박용규,박형서,노상필,정승현,김정희,이재환,최시완,성인환 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

급성 심근염환자에서 발행한 완전 방실차단을 영구 심장박동 조율기로 치료한 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Myocarditis, an inflammatory process involving the myocardal wall, may be caused by most infectious agents. Irrespective of its etiology, it presents commonly with evidence of heart failure, hypotension, and various electrocardiographic abnormalities. Patient with myocarditis may be asymptomatic or may have a rapidly progressive fatal disease. Medical management of patients with myocarditis includes specific therapy for underlying infection and control of the complication of myocarditis such as congestive heart failure and arrhythmia. Recently we had experienced myocarditis with complete atrioventricular block in 37-year-old man. Although he had shown complete recovery of heart failure, he had shown complete atrioventricular block, so we inserted permanent pacemaker. We report it with review of the literature.

방사선치료시 종양선량 예측 프로그램에 관한 연구

김유현,김창균,박장필 高麗大學校 倂設 保健大學 保健科學硏究所 2003 保健科學論集 Vol.29 No.1

The aim of this study is to develop a computer program which computes in-vivo doses from transmission doses measured during patient treatment using an ionization chamber. As the Kim's papaer, We adopted a straightforward empirical approach using a phantom transmission factor (PTF) which accounts for the contribution from all three factors. We develop the program which computes tumor doses by Microsoft Visual Basic Application program for D_(center) = D_(t)/PTF(d_(g), T_(p))×(SCD/SAD)²× BSF(r_(o))× S_(p)(r_(p,eff)) × TMR(d, r_(p,eff)). To make certain the accuracy of this program, we checked the accuracy for the following cases; in cases of regular or irregular field size, inhomogeneous material included. The errors were within 0.7% for regular field size, 2.5% irregular field size, 3.8% when inhomogeneous material was included in the phantom. It is considered that this program can make the quality control for dose at the time of radiation therapy because it is non-invasive that makes possible to measure the doses whenever a patient is given a therapy as well as eliminates the problem for entrance or exit dose measurement.

기능적 방법을 이용한 소아의 하악과두 골절의 처치 : 증례 보고 A case report

이정하,박성규,위유민,정현구,이봉규,이창섭 조선대학교 구강생물학연구소 2002 Oral Biology Research (Oral Biol Res) Vol.26 No.2

The pediatric condylar fracture occurs very frequently in the mandible, but this injury is occasionally ignored due to difficulty of diagnosis and no cooperation of patient. The adequate initial diagnosis and active treatment must be performed because delayed and improper treatment lead to possible severe complication such as TMJ ankylosis and retardation of mandibular development. Most pediatric condylar fracture is mainly performed by the conservative or functional treatment. but it may be required open reduction according to cases. In this study, Activator is applied for functional treatment of pediatric condylar fracture and fair recovery of jaw function is acquired.

보신 관광에서 감염된 수입성 Sparganosis 1예 : Intake of Thailand Snake during Trip

서환조,박명재,고경식,백영한,조유정,양현종,류경남,정형근,전정열 대한감염학회 1992 감염 Vol.24 No.1

Human sparganosois was reported more than 100 cases in Korean population. However no case reported yet in Korea as a patient was infected by sparganum from other geographical area, such as Thailand. The case we are reported here is a 38 year-old Korean male who had been suffered from mass being a small, round and soft consistance in upper portion of his right thigh. The patients stated that he had an opportunity to eat living snake in Bangkok, Thailand during trip. As therapy praziquantel (75㎎/㎏/day) was given p.o. for 14 days, but in vain to cure. Following medical treatment, surgical exicision was performed to remove a alive, soft, whitish-milky, mobile and elongated sparganum. Serlogical test by micro-ELISA for sparganum-specific antibody revealed a high serum antibody level of absorbance 0.67 (positive criterion: absorbance 0.22).