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자궁경부 상피종양에서 human papillomavirus 감염과 survivin 발현
윤재호,정동준,이정은,박동명,배동한,선우재근,백무준,김창진 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1
Human papillomavirus (HPV) has been considered a causative agent of uterine cervical carcinoma. HPV is a DNA oncogenic virus, which is well known as a causative virus in uterine cervical carcinoma. The virus is classified into two groups genotypically, low risk and high risk, according to the carcinogenic potentiality, and the determination of the viral genotype is important in clinical practice. Recently, numerous genotypes can be determined by high throughput method using DNA chip. Survivin is a recently characterized inhibitor of anti-apoptosis (IAP) protein, which is abundantly expressed in most solid and hematological malignancies, but undetectable in normal adult tissues. In this study, HPV genotypes are determined by DNA chip and the expression of survivin was examined by immunohistochemistry in 80 cases of uterine cervical intraepithelial neoplasia (CIN) and invasive carcinoma to see the roles of HPV and survivin in the carciogenesis of uterine cervical epithelial neoplasia. The results were as follows: 1. HPV positive rate was 72.5%, while negative rate was 27.5% in 80 cases of CIN and invasive carcinoma. The CIN and invasive carcinoma showed higher HPV positive rate (p <0.05). 2. HPV positive rate according to the histologic grade were 60%, 65%, 77% and 90% in CINI, CINII, CINII and invasive carcinoma, respectively. HPV positive rate showed increasing tendency according to the histologic grade, though there was no statistical significance. 3. The most frequent genotype was type 16 and the next were 58, 52, 18 and 33 in order of frequency. 4. Survivin was expressed in 96.3% of CIN and invasive carcinoma. The expression rate of survivin showed no significant difference between the histologic grade of CIN and invasive carcinoma, but showed tendency of increased expression rate in invasive carcinoma. 5. Survivin was expressed in HPV positive and in HPV negative each as in 95.5% and 96.6% respectively. There was no significant difference of survivin expression between HPV positive and negative cases. The above results suggest that HPV has no effect on the regulation of survivin expresson level in the uterine cervical intraepithelial neoplasia and invasive carcinomas.
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백일현,윤여일,김종기,박정훈 한국공업화학회 2005 응용화학 Vol.9 No.1
A pelletized absorbent was developed in order to separation the CO₂ generated during methane steam reforming reaction. The best adsorbent manufactured using the organic and inorganic binder showed the best plasticity when CaCO₃, feldspar and bentonite are mixed at the rate of 90:5:5% with the hardness of 95%. The CO₂ breakthrough test on the adsorbent using the fixed bed reactor found that the amount of adsorption was very high at the reaction temperature of 750>700>800℃ in the descending order.
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백일현,윤여일,김종기,박정훈 한국공업화학회 2005 응용화학 Vol.9 No.1
Based on the results coming from the methane steam reforming and the CO₂ breakthrough experiments, the basic design of the hybrid reaction process was set up. Hybrid reaction test using the device of the lab and bench size revealed high hydrogen and low CO₂ composition compared with the equilibrium composition of the methane steam reforming. In particular, an examination of the average composition through the repeated experiments of the hybrid reactions at the maximum adsorption temperature of the adsorbent of 750℃ found that hydrogen had 12 to 14% higher composition in the hybrid reaction compared with the methane steam reforming whereas the composition of CO₂ was 6-8% lower.
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대구 및 인근지역의 도로먼지에 함유된 미량원소성분의 함량분포
황윤정,황승만,최진수,김무식,백성옥 嶺南大學校 環境問題硏究所 1998 環境硏究 Vol.17 No.2
요약문 본 연구는 각종 먼지 가운데서도 독성이 가장 큰 것으로 알려진 도로먼지에 함유된 미량원소 성분이 조성 및 특성을 파악하기 위해 1992년 1월에서 10월가지 주거지역, 상업지역, 공업지역, 교외지역, 전원지역 등에 위치한 도로에서 시료를 채취하였으며 채취된 시료는 산으로 추출한후 ICP로 분석하였다 . 그 결과 토사 및 황사현상과 같은 자연발생원과 연관지을 수 있는 Al, Ca, Mg, Ti, Si, Na, K의 농도가 지역에 따라서는 전원지역과 교외지역에서, 그리고 도로포장 형태에 따라서는 비포장 도로에서 높게 나타난 반면에, 각종 연소 및 차량과 같은 인위적인 발생원과 관련이 있는 As, Cr, Mn, Zn, Pb, Cu, Ni는 공업지역 및 상업지역, 그리고 아스팔트에서 비교적 높게 검출되어 이러한 미량원소성문들의 농도분포는 지역에 따라, 그리고 도로포장 형태에 따라 특징적인 양상을 나타내는 것으로 조사되었다. 또한 As, Pb, Al 등의 일부 미량원소성분들의 농도는 계절에 따른 차이를 나타내었으며, 농축계수에 의한 평가결과 농축계수가 낮게 나타난 Na, Si, Ti, K, Al 등은 자연발생원에 의해 상당한 영향을 받는 반면에 As, Pb, Zn 등은 농축계수가 매우 높게 나타나 이들 성분의 경우에는 인위적인 발생원에 의한 기여도가 큰 것으로 평가되었다. Abstract This study was carried out to characterize the trace elements in street dust known to contain several toxic elements. Street dust was collected in residential, commercial, industrial, suburban and rural area of Taegu and on the vicinity of Taegu from January to October, 1992. Total amount of 16 trace elements in street dust were analyzed by ICP spectrophotometer. While the levels of As, Cr, Mn, Zn, Pb, Cu and Ni were higher in industral and commercial area than any other area by sites and the highest levels appeared on asphalt and cement street by street conditions. Also the concentrations of some trace elements originated mainly from soil (e.g. Al, Ca, Mg, Si, Na) were higher in rural and suburban area by sites and the highest levels appeared in unpaved street by street conditions. The concentrations of several trace elements such as Aa, Pb, Al appeared seasonal differences. The estimated enrichment factors (EF) for Na, Si, Ti, K and Al components showed with soil compositions appeared to have low values, while As, Pb and Zn components showed relatively higher values. These results imply that the latter constituents were likely to be affected by dry and wet depositions of atmospheric particles orginated from anthropogenic sources such as fuel combustion and refuge burning.
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범가자미, Verasper variegatus의 난황단백질 분리 및 면역학적 특성
백혜자,한창희,김우진,박중연,김진한,김윤 동의대학교 기초과학연구소 1998 基礎科學硏究論文集 Vol.8 No.1
The yolk protein of spotted flounder, Verasper variegatus was purified by precipitation with cold distilled water, followed by Sepharose CL-6B column chromatography. The purified protein was identified as vitellin by Ouchterlony's immunodiffusion test and immunoelectrophoresis. The purified vitellin from ovarian crude extracts has same antigenic determinants with the female specific serum protein, vitellogenin. The molecular weight of purified vitellin was estimated about 550kD by gel filtration. The vitellin was composed of three major subunits with molecular weight of about 108, 85 and 31 kD, and two minor subunits. The vitellin was identified by western blot analysis using anti-vitellin antibody.
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노인 인구에서 신경인지기능저하와 혈청 지질농도와의 상관성
유영선,최석주,정성수,김지은,윤성욱,전동욱,백준형,박성우,이정구,추일한,김영훈 大韓神經精神醫學會 2008 신경정신의학 Vol.47 No.6
Objectives : In this study, the authors evaluated the correlation between eurocognitive impairments and serum lipids levels among Korean elderly over the age of 65. Methods : A total of 609 elderly individuals participated in this study. Screening for cognitive impairments were carried out using the Mini-Mental Status Examination-Korean version (MMSE-KC). There were 197 subjects above 1.5SD Of MMSE- KC and they were evaluated using the Korean versions of the Consortium to Establish a Registry for Alzheimer's Disease (AD) (CERAD-KC) and Geriatric Depression Scale (GDS-K). Results : There was a correlation between old age/low levels of education and AD (p<0.05, P<0.01). There was an inverse correlation between the serum level of total cholesterol and the word list recognition test scores. The serum levels of LDL cholesterol were inversely correlated with the scores on the constructional praxis test, word list recall test and word list recognition test. Inverse correlations between the serum level of triglyceride and scores on the word list recall test and word list recognition test were also observed. Conclusion : There were inverse correlations between the serum levels of lipids and language/memory function in subjects with AD.
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주지현,최정현,이동건,백지연,고윤호,이혜정,김세희,신호진,박윤희,박지영,김유진,신완식,김춘추 대한감염학회 2001 감염 Vol.33 No.4
Background : Pneumocytitis cainii pneumonia (PCP) can occur in immunocompromised hosts especially such as AIDS or cancer patients. Although recent research had focused on PCP in AIDS patients, few studies have described the clinical presentations of PCP in recipients of stem cell transplantation (SCT). We evaluated the clinical manifestations of PCP in SCT patients admitted at St. Mary's hospital, Seoul, Korea. Methods : The medical records of 17 PCP patients undergoing SCT between Feb. 1998 and Feb. 2000 were reviewed. The diagnosis of PCP was confirmed through the demonstration of Pneumocytitis cainii via either cytology of brochoalveolar lavage (BAL) or histological technique of lung biopsy. CMV disease and CMV infection were confirmed by BAL culture and antigenemia respectively . Results : Seventeen patients were all recipients of allogeneic SCT and 7 of 17 patients were performed non-sibling SCT. Patients presented with symptoms including brief period (4 ∼23 days) of fever (76%), dyspnea (70%), cough (64%), and signs such as rare(58.8%), Sixteen patients (94%) had been receiving immunosuppressive agent such as cyclosporine A (64%) or Fk506 (35%) without PCP prophylaxis. Eleven patients (64%) were treated with corticosteroid with mean dose of 16 mg/day prednisolone and mean duration of 4.6 months after post-SCT period. Twelve patients were co-infected with CMV. Another co-infected miCroorganisms were Pseudomonas aeruginosa, Mycobacterium tuberculosis, herpes simplex virus, parainfluenza virus, Average duration of treatment with trimethoprim-sulfamethoxazole (TMP/SMX) was 21 ±9 days. Four patients died, and three of them were related with PCP. Conclusion : PCP developed frequently in patients who were taking immunosuppressive drug due to graft versus host disease or were not taking TMP/SMX prophylaxis. High risk patients showing fever, cough, or dyspnea should be considered to take early bronchoscopic intervention for detection of PCP. When treat for PCP, it also be considered to the possibility of coinfection such as CMV. (Korean J Infect Dis 33:273∼279, 2001)
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