Choline dehydrogenase interacts with SQSTM1/p62 to recruit LC3 and stimulate mitophagy.

Park, Sungwoo,Choi, Seon-Guk,Yoo, Seung-Min,Son, Jin H,Jung, Yong-Keun Landes Bioscience 2014 AUTOPHAGY Vol.10 No.11

<P>CHDH (choline dehydrogenase) is an enzyme catalyzing the dehydrogenation of choline to betaine aldehyde in mitochondria. Apart from this well-known activity, we report here a pivotal role of CHDH in mitophagy. Knockdown of CHDH expression impairs CCCP-induced mitophagy and PARK2/parkin-mediated clearance of mitochondria in mammalian cells, including HeLa cells and SN4741 dopaminergic neuronal cells. Conversely, overexpression of CHDH accelerates PARK2-mediated mitophagy. CHDH is found on both the outer and inner membranes of mitochondria in resting cells. Interestingly, upon induction of mitophagy, CHDH accumulates on the outer membrane in a mitochondrial potential-dependent manner. We found that CHDH is not a substrate of PARK2 but interacts with SQSTM1 independently of PARK2 to recruit SQSTM1 into depolarized mitochondria. The FB1 domain of CHDH is exposed to the cytosol and is required for the interaction with SQSTM1, and overexpression of the FB1 domain only in cytosol reduces CCCP-induced mitochondrial degradation via competitive interaction with SQSTM1. In addition, CHDH, but not the CHDH FB1 deletion mutant, forms a ternary protein complex with SQSTM1 and MAP1LC3 (LC3), leading to loading of LC3 onto the damaged mitochondria via SQSTM1. Further, CHDH is crucial to the mitophagy induced by MPP+ in SN4741 cells. Overall, our results suggest that CHDH is required for PARK2-mediated mitophagy for the recruitment of SQSTM1 and LC3 onto the mitochondria for cargo recognition.</P>

홍삼류의 섭취가 비만과 혈중 지질의 상호관계에 미치는 영향

박화진,이정희,이소진,함혜선,조현정,임창률,유영빈,박기현 대한의생명과학회 2000 Journal of biomedical laboratory sciences Vol.6 No.4

비만은 동맥경화의 위험인자로서 혈중에 triglyceride의 농도를 종가시키고, 상대적으로 HDL-Cholesterol의 농도를 감소시킴과 동시에 수축기 혈압을 상승시킨다. 본 연구에서 홍삼제품류를 4년 또는 5년 동안 복용해 온 건강한 사람 (ginseng군)과 홍삼제품을 복용하지 않은 건강한 사람 (control군)을 대상으로 하여 신체 계측치로부터 비만지수를 구하고 이것과 혈중의 triglyceride (TG)농도, TG/HDL-Cholesterol ratio 및 수축기 혈압과의 상호관계를 연구한 바, ginseng군에서는 대조군에 비해 혈중의 TG 농도가 일정하게 유지되었고, TG/HDL-Cholesterol ratio 및 수축기 혈압도 일정하게 유지되었다. 이러한 현상은 ginseng군에서 기호품으로 alcohol을 섭취하거나, 흡연을 한 경우에도 상관없이 일정하게 유지 되었다. 이 결과는 홍삼제품류를 장기 복용하면 비만의 저하 및 고혈압 또는 동맥경화의 위험인자가 억제될 수 있고, 결론적으로 비만, 고혈압 및 동맥경화를 예방할 수 있을 것으로 추정한다. Obesity is the risk fcactor of atherosclerosis and not only increases triglyceride concentration in blood but also decreases relatively the ratio of TG to HDL-Cholesterol in blood. In case of obesity, systolic blood pressure is also increased in responding the increase of TG in blood. Index of obesity in red ginseng-taking group (ginseng group) was lower as compared with non-red ginseng-taking group (control group). The TG concentration, the ratio of triglyceride to HDL-cholesterol in blood and systolic blood pressure were decreased in the subjects of ginseng group compared with that in control group. It is inferred that long-term intake of ginseng products may help to prevent the risk of atherosclerosis and obesity.

Oyster-Zeolite 흡착탑을 이용한 하수의 유기물질과 질소제거

정병길,정유진,박성현,최호은,성낙창 東亞大學校 建設技術硏究所 2005 硏究論文集 Vol.29 No.1

The objective of this study is to investigated the removal possibility of organic matter and nitrogen contained final effluent of sewage treatment plant using oyster-zeolite adsorption column with waste oyster shell and natural zeolite. The raw water was collected the final effluent of Janglim sewage treatment plant in Busan city. The organic matter and T-N removal efficiency ranged from 2~60% and 38~97%, respectively. In conclusion, oyster-zeolite adsorption column could organic matter and nitrogen removal of final effluent of sewage treatment plant and its economical efficiency is enough because of using recycled waste oyster shell and a cheap natural zeolite.

가시오가피 투여가 혈중 전해질농도에 미치는 영향

유창재,박정순 신라대학교 자연과학연구소 2000 自然科學論文集 Vol.8 No.-

The purpose of this study was to investigate the effect of Acanthopanax teas(by Sang-ill medicine manufacture) on the concentration of electrolyte. The subjects employed were 4 male students who are majoring in physical education and 3 female students who are majoring in dance. Acanthopanax teas were orally administered 3 package/day for 1 month. The results of this study were as follows. 1.There were significant increase in Ca concentration at static state, immediately after the maximal exercise, 5 and 10 min of recovery period, as well. 2.There were no significant differences in Cl concentration at static state, immediately after the maximal exercise, 5 and 10 min of recovery period. 3.In static state, K concentration was increased by Acanthopanax teas but there were no significant differences at immediately after the maximal exercise, at 5 and 10 min of recovery period, as well. 4.There were no significant differences in Mg concentration at static state, immediately after the maximal exercise, 5 and 10 min of recovery period. 5.There were no significant differences in Na concentration at static state, immediately after the maximal exercise, 5 and 10 min of recovety period.

횡성군에서의 자살에 관한 연구

박기창,한정옥,유계준 大韓神經精神醫學會 1985 신경정신의학 Vol.24 No.2

An epidemiologic and psychiatric studies about suicides in Whoeng-Sung county for the past 7 years from January 1, 1977 to December 31, 1983 were conducted in order to identify overall pictures of suicides committed in this area. All the suicidal cases were obtained from the records of Whoeng-Sung police station. The authors visited homes of suicide victims and interviewed the family members, the relatives and the neighbors. The total number of suicides committed in this area during the study period was 123, and the suicidal rate was 23.4 per 100,000 population. The number, excluding the inhabitants outside this area, was 109, and the rate was 20.7 per 100,000 population. The male to female ratio was 2 to 1 and the highest rates were shown in the age group of 50-59 for male and 20-29 for female. Also, higher rates were shown in lesser educated, lower economic class, and people of Buddism and Confucianism. The main method for suicides, accounting for 76.2% of the total, was by ingestion of herbicides and insecticides for farming purposes. The place selected for the suicide was usually at victim's own house (67.9%). 82.4% of the victims have previously suggested their intention to commit suicide and of the victim have left a will. Also the suicidal rate was highest in the summer, higher in the afternoon, and higher in the beginning of the week for the male. Single men showed the highest suicidal rate. The common precipitating factors of committing suicide were family trouble, chronic illness or disability and financial difficulty. The most apparant dynamic motives of the suidides was easy self-abandonment of one's own life. Most of the suicidal victims had some psychopathologic condition (91.6%).

플라이 애쉬가 콘크리트 구조물의 철근 부식에 미치는 영향

박진택,유지홍,김정구,이재호,이광명 대한금속재료학회 2003 대한금속·재료학회지 Vol.41 No.4

Addition of fly ash has beneficial effects on some mechanical properties of concrete, as well as the corrosion process induced by the chloride ion. The aim of this study was to investigate the effect of fly ash on the steel corrosion in concrete with various water-to-binder ratios. The corrosion process of steel bars embedded in concrete without fly ash and with 20% fly ash was tested under complete immersion, in 3.5 wt.% NaCl solution. Monitoring of open-circuit potential and linear polarization method and electrochemical impedance spectroscopy(EIS) were used to follow the corrosion process. Also, ASTM C1202 test method was used to estimate the concrete's resistivity of chloride penetration in concrete. The results obtained from electrochemical tests show that the use of fly ash has led to the improvement of corrosion resistance. Partial cement replacement by fly ash caused significant pore refinement, reduced permeability to chloride ions, and increased corrosion resistance.

Chlamydia trachomatis에서 분리된 Cryptic plasmid DNA의 클로닝

유정한,김덕언,박일규,강정옥,최태열 대한임상병리학회 1996 대한임상병리학회지 Vol.16 No.5

웹기반에서의 성교육을 위한 코스웨어 설계 및 구현

유정숙,박두순 순천향대학교 부설 산업기술연구소 2002 순천향 산업기술연구소논문집 Vol.8 No.1

The recent development of a high-speed Internet network has greatly advanced the learning methods based on computers. Especially, the advent of the World Wide Web has changed the traditional face-to-face teaching methods by allowing a new way the educators and educates can communicate with each other anytime and anyplace. The purpose of this study is to help students build a proper view of value about 'sex', and desirable human relations by changing misinformation about sex and sexual indentity into a positive, sound direction of thinking about 'sex' through a web based educational program.

보조공학기기 이용실태 및 서비스 희망에 대한 심층면접

정민예,김정란,양노열,유인규,박혜연 대한보조공학기술학회 2009 대한보조공학기술학회지 Vol.1 No.1

목적 : 구직장애인, 장애인공무원과 지원을 많이 받아보지 않은 사업주를 대상으로 심층면접을 통해 보조공학기기 이용실태 및 서비스 희망을 조사하고, 이를 통하여 앞으로 보조공학서비스 서비스 방향을 제안하고자 조사하였다 연구방법 : 심층면접의 대상자는 장애인공무원, 구직장애인, 사업주로 분류하여 반구조화된 가이드라인 설문을 구성하였다. 면접 대상은 설문조사를 위한 모집단 중 무작위로 추출하여 면담 조사에 협조를 약속한 대상자 사업주 7명, 구직장애인 7명, 장애인공무원 5명을 대상으로 총 19명이 응답에 참여하였다. 결과 : 수집된 자료는 질적 분석 절차를 통해 보조공학기기의 사용경험, 보조공학기기 신청경험 또는 개인적 구입경험, 장애유형에 관련된 보조공학기기의 사용경험여부 및 인식조사, 보조공학서비스에 대한 인식정도 및 이용경험과 보조공학서비스 방향과 추가로 제공을 희망하는 서비스 내용 5가지의 주제가 도출되었다. 결론 : 보조공학서비스의 보다 효율적인 운영방식을 고려해야 할 것이며, 보조공학서비스를 할 때 구입이나 사후 관리도 보다 사용자 중심에서 서비스 제공이 필요할 것이다. Objective : The purpose of this study was to provide the information about the actual demand and use of assistive technology among people with disabilities who not received assistive technology service. Methods : In-depth interview subjects were government officials with disable, disabled job seekers, and employers hiring workers with disabilities. We made up the semi-structure guidelines question with group into three classes. We asked for an interview with subjects that choose samples at random. Results : These results show five themes related to use and application experience in assistive technology, the awareness of assistive technology service, and the demand to make of assistive technology service. Conclusion : In conclusion, we suggests that they want to be included assistive technology services target and replied use of those assistive devices will help them to get a job or to improve work efficiency and capacity.

사망진단서(사체검안서) 상의 선행사인으로부터 사망통계의 원사인이 선정되는 비율 : 3개 대학병원에서 교부된 사망진단서를 중심으로

박우성,박석건,정철원,김우철,탁우택,김부연,서순원,김광환,서진숙,부유경 한국의료QA학회 2004 한국의료질향상학회지 Vol.11 No.1

Background : To exatnine the problems intolved in writing practice of death certificates, we cotnpated the determination of underlying cause of death for wital statistics using recorded underlying cause of death in issued death statistics. Methods : We collected 688 rnortality certificates issue in year of 2,000 from 3 university hospitals. And we also collected vital statistics from ministry of statistics. The causes of death were coded by experienced medical record wpecialists. And causes of death determined at ministry of statistics for national vita statistics were mapped to causes of death recorded at each death certificates. The rate that underlying causes of death for vital statistics were derived from underlying causes of death recorded at issued death certificaties sere analysed. Results : 64.5% of underlying cause of death for could be derived from underlying cause of death recorded at issued death certificates, 8.6% derived from intermediate cause of death, and 3.9% derived from direct cause of death. In 23% of cases, underlying cause of death could not be derived using issued death certificates. The rate that underlying cause of death for vital statistics could be derived from underlying cause of death recorded at death certificates was different between 3 university hospitals. Ane the rate was also different between death certificates and postmortem certificates. We classified the causes of death using 21 major categories. The rate was different between diseases or conditions tha caused death too. Conclusion : When we examined the correctness of death certificate writing practice using abpve methods, cortectness of writing could not be told as satisfactory. There was difference in correctness of writing between hospotals, between death certificates and postmortem certificates, and between diseases and conditions that caused death. With this results, we suggested some strategy to improve the correctness of death certificate writing practice.