정신분열병 환자에서 세가지 청각 자극 "Oddball" 모형에 의한 사건관련전위 P3a와 P3b

진용탁,박이진,남지민,한상익,전양환 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.5

Objectives : Using 3-stimulus auditory "oddball" paradigm reflecting fronto/central and temporo/parietal functions Simultaneously, we examined patients with schizophrenia. Methods : fifteen patients with schizophrenia from outpatient clinic and fifteen normal controls from hospital staffs were recruited for the study. To elicit P3a and P3b, 3-stimulus auditory oddball paradigm was employed. The 3-stimulus auditory oddball paradigm was composed of standard tone (1,000 Hz, 75 dB, 80%), target tone (2,000 Hz, 75 dB, 10%) and distracter (White noise, 95 dB, 10%). Results : P3a and P3b were prominent in fronto/central and temporo/parietal areas, respectively, in both schizophrenics and normal controls. The P300 amplitude in patients with schizophrenia was reduced across P3a and P3b (p<0.01). The P300latency in patients with schizophrenia was delayed across P3a and P3b (p<0.01). Conclusion : These results were consistent with frontal and temporo-parietal lobe dysfunctions in schizophrenics. The 3-stimulus auditory paradigm could be applied for patients with schizophrenia and useful for further exploration the disorder.

Polyozellus multiplex가 생산하는 지질과산화 저해물질

황지숙,송경식,김양섭,석순자,이태호,유익동 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.5

담자균류로부터 지질과산화 저해물질을 탐색하던 중 강원도 오대산 지역에 자생하는 버섯의 한 종류로 부터, Sparague-Dawley계 rat의 간 mirosome 분획의 지질과산화를 강하게 억제하는 물질이 있음을 발견하였다. 이 버섯의 미생물학적 특성을 관찰한 결과 민주름버섯목, 굴뚝버섯과에 속하는 까치버섯(Polyzellus multiplex)으로 동정하였으며 본 버섯의 자실체 methanol 추출물을 benzene, ethylacetate로 순차적으로 추출하고 각종 chromatography를 이용하여 활성물질을 정제한 결과, ethylacetate층으로 부터 황산화활성을 나타내는 PM1을 benzene층으로 부터 지방산 계열의 PM2와 PM3 화합물을 순수하게 분리 정제하였다. PM1은 정색반응 및 각종 기기분석 결과 thelephoric acid계의 화합물로 동정되었으며 PM2 및 PM3는 각각 linoleic acid와 oleic acid로 동정되었다. 지질과산화 활성물질로 분리정제한 PM1 화합물의 IC_50 값은 1.96 ppm이었으며 LD_50 값은 500 mg/Kg 이상이었다. In the course of screening lipid peroxidation inhibitor from basidiomycetes, a mushroom, which was collected at O-Dae mountain in Kangweon-Do, was found to have active compound. The mushroom was identified as Polyzellus multiplex, which belongs to Aphylloporalles Thelephoraceae, on the basis of macroscopic and microscopic characteristics of the fruiting body. The methanol extract of fruiting body was extracted with benzene and ethylacetate, sequentially. By using various kinds of chromatographies, PM1, and PM2 and PM3, were purified from the ethylacetate extract and the benzene extract, respectively. Color reaction and analyses of IR, UV, and NMR spectra indicated that PM1 was a derivative of thelephoric acid, and PM2 and PM3 were linoleic acid and oleic acid, respectively. IC_50 of PM1 for inhibition of lipid peroxidation was 1.96 ppm and LD_50 was 500 mg/kg.

ANTITUMOR ACTIVITIES OF EXOPOLYSACCHARIDE PRODUCED BY Ganoderrna lucidum MYCELIUM

Yong Ick Ji,Soon Ok Moon,Shin Young Lee 생화학분자생물학회 1996 생화학분자생물학회 춘계학술발표논문집 Vol.- No.-

Clinical characteristics and outcomes of lung adenocarcinoma with solid or micropapillary subtypes

( Sun Ha Choi ),( Jae Yong Park ),( Yong Seon Yoon ),( Hee Won Kwon ),( Ji Eun Park ),( Sun Ji Park ),( Yong Hoon Lee ),( Hyewon Seo ),( Seung Soo Yoo ),( Shin Yup Lee ),( Jaehee Lee ),( Seung Ick Cha 대한결핵 및 호흡기학회 2019 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.127 No.-

Objective: The aim of this study was to investigate the clinical characteristics and outcomes of lung adenocarcinoma with solid or micropapillary (S/MP) subtypes in surgically treated patients. Methods: We retrospectively reviewed medical records and pathologic reports of 410 patients with lung adenocarcinoma who underwent curative resection. Clinical features and recurrence free survival were compared between groups with S/MP subtypes (S/MP+, n=203) and without S/MP subtypes (S/MP-, n=207). Result: S/MP subtypes were present in 203 patients (49.5%), and 141 (34.4%) had ≥ 5% of S/MP proportion. S/MP subtypes were more frequently observed in patients with male gender (57.6% vs. 42.0%, P=0.002), tumor > 3cm (62.3% vs. 37.4%, P<0.001), and positive lymph node stasis (85.9 vs. 40.9%, P<0.001) compared to female gender, tumor ≤ 3cm, and negative lymph node stasis, respectively. Recurrence and death were more frequent in S/MP+ group compared with S/MP- group (24.1% vs. 1.9%, P<0.001 and 5.4% vs. 1.4%, P=0.02). The tumor with S/MP proportion of < 5% (S/MP< 5%) were more frequent in patients with tumor > 3 cm (34.8% vs. 14.3%, P<0.001) and positive lymph node metastasis (60.9% vs. 18.7%, P<0.001) compared to tumor ≤ 3cm and negative lymph node metastasis, respectively, and more frequently associated with recurrence and death (22.6% vs. 1.9%, P<0.001 and 6.5% vs. 1.4%, P=0.05) compared with S/MP- group. Survival analysis indicated that S/ MP+ and S/MP<5% were associated with shorter recurrence free survival compare with S/MP- (HR=9.1, 95% CI=3.1-26.8, P<0.001; and HR=10.1, 95% CI=2.9-35.5, P<0.001, respectively). S/MP+ and S/MP<5% were more powerful predictor of recurrence than T or N stage in multivariate analyses.

Associations between $DRDs$ and schizophrenia in a Korean population: multi-stage association analyses

Lee, Kyu-Young,Joo, Eun-Jeong,Ji, Yong-Ick,Kim, Duk-Hwan,Park, Joo-Bae,Chung, In-Won,Lee, Sang-Ick,Joo, Yeon-Ho,Ahn, Yong-Min,Song, Joo-Yun,Kim, Yong-Sik Korean Society for Biochemistry and Molecular Bion 2011 Experimental and molecular medicine Vol.43 No.1

The dysregulation of the dopaminergic system has been implicated in the pathophysiology of major psychosis, including schizophrenia, with dopamine receptor genes ($DRDs$) presently targeted as the most promising candidate genes. We investigated $DRD1-5$ for association with schizophrenia using a multi-stage approach in a Korean sample. One hundred forty-two SNPs in $DRD1-5$ were selected from the dbSNP, and the associations of each SNP were then screened and typed by MALDI-TOF mass spectrometry using pooled DNA samples from 150 patients with major psychosis and 150 controls. Each of the suggested SNPs was then genotyped and tested for an association within the individual samples comprising each pool. Finally, the positively associated SNPs were genotyped in an extended sample of 270 patients with schizophrenia and 350 controls. Among the 142 SNPs, 88 (62%) SNPs in our Korean population were polymorphic. At the pooling stage, 10 SNPs ($DRD1$: 2, $DRD2$: 3, and $DRD4$: 5) were identified ($P$ < 0.05). SNPs rs1799914 of $DRD1$ ($P$ = 0.046) and rs752306 of $DRD4$ ($P$ = 0.017) had significantly different allele frequencies in the individually genotyped samples comprising the pool. In the final stage, with the extended sample, the suggestive association of $DRD4$ with rs752306 was lost, but the association of $DRD1$ with rs1799914 gained greater significance ($P$ = 0.017). In these large-scale multi-stage analyses, we were able to find a possible association between $DRD1$ and schizophrenia. These findings suggested the potential contribution of a multi-step strategy for finding genes related to schizophrenia.

혈중 고 알칼리인산분해효소치를 보인 영아에서 혈중 비타민 D 농도에 대한 연구

김지성,최재용,이균우,송익진,김철암,손병희,이정현 고신대학교의과대학 2012 고신대학교 의과대학 학술지 Vol.27 No.1

Objectives: Nowadays most infants on exclusively breast feeding have vitamin D deficiency due to the increase of breast feeding. However, domestic research lacks appropriate materials. Therefore, we researched practical clinical aspects of vitamin D deficiency related to breast milk feeding for infants who have a high amount of alkaline phosphatase (> 500 IU/L). Methods: The subjects of the study were 31 infants with high alkaline phosphatase level. We tested with 25-hydroxycholecalciferol (25-OHD3), parathyroid hormone, calcium, ionized calcium, phosphorus in their blood and with a wrist x-ray. Then, we divided them into two groups of breast feeding and formula feeding and compared the results. Results: Eighteen infants (58%) out of 31 infants that have high alkaline phosphatase were vitamin D insufficiency or deficiency, and 16 (100%) breast feeding infants of them showed vitamin D deficiency or insufficiency. However, only 2 (13%) of 15 formula feeding infants were at insufficiency. There was a correlation between alkaline phosphatase and 25-OHD3 concentration in multiple regression analysis, but no correlation in other variables was found in group of breast milk feeding infants. There was neither correlation between vitamin D concentration and alkaline phosphatase nor other correlated variables in the group of formula milk feeding infants. Conclusions: In this study, there was a high probability of vitamin D deficiency in the breast feeding infants with a high alkaline phosphatase level. Therefore, it is considered to be worth utilizing alkaline phosphatase as a screening test for vitamin D deficiency or rickets for breast feeding infants.

Role of Chest Computed Tomography in Patients Hospitalized with Community-acquired Complicated Parapneumonic Effusion or Empyema

( Ji-eun Park ),( Sunji Park ),( Sun Ha Choi ),( Hyewon Seo ),( Yong-hoon Lee ),( Seung-soo Yoo ),( Shin-yup Lee ),( Jaehee Lee ),( Chang-ho Kim ),( Jae-yong Park ),( Seung-ick Cha ) 대한결핵 및 호흡기학회 2020 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.128 No.0

Background Data regarding predictors of the outcome for patients with community-acquired complicated parapneumonic effusion (CPPE) or empyema are insufficient. Method Patients with community-acquired pneumonia (CAP) were classified into CPPE or empyema and control groups based on pleural fluid analysis and microbiological data. The patients with CPPE or empyema were further divided into longer and shorter length of stay (LOS) groups, and clinical characteristics, pleural fluid data, and computed tomographic (CT) findings were compared between the two groups. Result Of outcome variables, LOS was significantly longer in CPPE or empyema group than in the control group (13 days [interquartile range, 10-17 days] versus 8 days [6-12 days], p < 0.001), whereas 30-day mortality and in-hospital mortality were not significantly different between the two groups. Patients with CPPE or empyema were divided into the shorter (≤ 13 days) and longer LOS (≥ 14 days) groups. Multivariate analysis demonstrated that pneumonia severity index (PSI) class IV-V (odds ratio [OR] 2.79, 95% CI 1.35-5.76, p=0.006), increased attenuation of extrapleural fat (OR 2.26, 95% CI 1.06-4.80, p=0.034), and air bubbles in pleural space (OR 3.93, 95% CI 1.03-14.98, p=0.045) were independent predictors of prolonged LOS in CAP patients with CPPE or empyema. Conclusion Increased attenuation of extrapleural fat and air bubbles in pleural space assessed with CT and PSI class IV-V independently predicted prolonged LOS in CAP patients with CPPE or empyema. These findings may be helpful to identify patients who need more intensive evaluation and intervention.

Clinical Implications of Ischemic Stroke in Patients with Pulmonary Embolism

( Yong Hoon Lee ),( Seung-ick Cha ),( Jongmin Park ),( Jae Kwang Lim ),( Won Kee Lee ),( Ji-eun Park ),( Sun Ha Choi ),( Hyewon Seo ),( Seung-soo Yoo ),( Shin-yup Lee ),( Jaehee Lee ),( Chang-ho Kim ) 대한결핵 및 호흡기학회 2021 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.129 No.-

The clinical implications of ischemic stroke on the outcomes of patients with pulmonary embolism (PE) are not well understood. We investigated whether the clinical characteristics and treatment outcomes differ according to the history of ischemic stroke in patients with PE. Patients hospitalized for PE were classified into the following two groups: patients with a history of ischemic stroke (stroke group) and those without prior stroke (control group). We compared the clinical, laboratory, and radiological parameters between the groups. Of the 1,339 patients with PE, 115 (8.6%) had a history of ischemic stroke. The proportion of unprovoked PE was lower in the stroke group than in the control group, whereas the frequency of immobilization was higher. Comorbidities, including diabetes mellitus, ischemic heart disease, and atrial fibrillation, were more common in the stroke group. Despite the insignificant difference between the two groups in PE severity index (PESI) and computed tomography (CT) findings, PE-related in-hospital mortality was significantly higher in the stroke group than in the control group (7 [6.1%] vs. 15 [1.2%], p = 0.002), and prior stroke was one of the independent factors predicting PE-related in-hospital deaths (odds ratio, 4.87; 95% confidence interval, 1.92-12.35; p < 0.001). In conclusion, although PESI score, right ventricular dilation on CT, and adverse outcomes did not differ between the stroke and control groups, a history of ischemic stroke might influence the PE-related in-hospital mortality in PE patients.

Butyrate-treated M2 Macrophage Polarization is CECR1-dependent

( Ji Eun Park ),( Ha Jeong Kim ),( Sunji Park ),( Sun Ha Choi ),( Yong Hoon Lee ),( Hye Won Seo ),( Seung Soo Yoo ),( Shin Yup Lee ),( Jaehee Lee ),( Seung Ick Cha ),( Jae Yong Park ),( Chang Ho Kim ) 대한결핵 및 호흡기학회 2020 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.128 No.-

Background The Cat Eye Syndrome Critical Region, Candidate 1 (CECR1) gene and its product adenosine deaminase 2 (ADA2) are highly expressed by macrophages. Patients with tuberculous pleural effusion (TPE) usually have elevated ADA2 levels in pleural fluid. In terms of immunomodulatory functions of intestinal metabolite butyrate, it may affect CECR1 expression in patients with TPE. We examined the effect of butyrate on CECR1 expression of macrophages and the role of CECR1 on butyrate-related immune response. Additionally, relationship between ADA2 and macrophage phenotype in pleural fluid of patients with TPE was investigated. Methods Expression of CECR1 was evaluated in LPS-stimulated and/or butyrate treated THP-1 cells. The role of CECR1 on butyrate-induced immune response was evaluated using siRNA transfected THP-1 cells. Correlation between the levels of chemokines associated with M1/M2 macrophage phenotype and ADA2 in pleural fluid of patients with TPE were analyzed. Results Butyrate promoted the expression of CECR1 and M2-macrophage marker in THP-1 cells. In LPS-stimulated THP-1 cells, butyrate facilitated M2-macrophage marker/anti-inflammatory cytokine expression and inhibited M1-macrophage marker/pro-inflammatory cytokine expression in a CECR1-dependent manner. Among M1 chemokines measured, there was a significant negative correlation between pleural fluid CCL21 and ADA2 levels and between CCL25 and ADA2 levels in patients with TPE (CCL21; Spearman r = -0.478, p = 0.050: CCL25; Spearman r = -0.808, p<0.001). Among M2 chemokines measured, there was a significant positive correlation between TGF-β and ADA2 levels and between IL-22 and ADA2 levels in pleural fluid of patients with TPE (TGF-β; Spearman r = 0.603, p<0.001: IL-22; Spearman r = 0.470, p = 0.050). Conclusions Butyrate enhanced M2-macrophage marker/anti-inflammatory cytokine expression of LPS-stimulated THP-1 cells in a CECR1- dependent manner. ADA2 may exert anti-inflammatory effect during the process of pleural inflammation in patients with TPE.

Genetic Polymorphisms in Activating Transcription Factor 3 Binding Site are Associated with the Prognosis of Early-stage Non-small-cell Lung Cancer

( Ji Eun Park ),( Shin Yup Lee ),( Hyo Gyoung Kang ),( Jin Eun Choi ),( Sun Ha Choi ),( Sunji Park ),( Yong Hoon Lee ),( Hye Won Seo ),( Seung Soo Yoo ),( Jaehee Lee ),( Seung Ick Cha ),( Chang Ho Kim 대한결핵 및 호흡기학회 2020 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.128 No.-

Background Transcription factor ATF3 plays a significant role in cancer development and progression. We investigated the association between the SNPs in eQTLs within ATF3 binding regions and the prognosis of NSCLC after surgery. Methods 89 SNPs were selected using publicly available web server (http://galaxyproject.org). Results Among those SNPs, HAX1 rs11265425T>G was associated with significantly worse DFS (aHR = 1.30, 95% CI = 1.00-1.69, P = 0.05), and the ME3 rs10400291C>A was associated with significantly better DFS (aHR = 0.66, 95% CI = 0.46-0.95, P = 0.03). According to tumor histology, the HAX1 rs11265425T>G was associated with significantly worse DFS (aHR = 1.45, 95% CI = 1.02-2.05, P = 0.04) only in adenocarcinoma (AC), and the ME3 rs10400291C>A was associated with significantly better DFS (aHR = 0.46, 95% CI = 0.25-0.86, P = 0.01) only in squamous cell carcinoma (SCC). ChIP-qPCR assays showed that rs11265425T>G and rs10400291C>A reside in active enhancers where H3K27Ac and ATF3 binding occurs. HAX1 RNA expression was significantly higher in tumor compared to non-malignant lung, and higher in rs11265425TG or GG genotypes than in rs11265425TT genotype. Conversely, ME3 expression was significantly lower in tumor than normal lung, and higher in rs10400291AA genotype than in rs10400291CC or CA genotypes. Conclusion n Conclusion, this study shows that the functional polymorphisms in ATF3 binding sites, HAX1 rs11265425T>G and ME3 rs10400291C>A are associated with the clinical outcomes of patients in surgically resected NSCLC. The analysis of those genetic variants may be useful for predicting patients’ prognosis after surgery, thereby helping to refine therapeutic decisions for better clinical outcomes in NSCLC.