Hepatitis B Virus DNA Polymerase Displays an Anti-Apoptotic Effect by Interacting with Elongation Factor-1 Alpha-2 in Hepatoma Cells

( Xianli Niu ),( Shirong Nong ),( Junyuan Gong ),( Xin Zhang ),( Hui Tang ),( Tianhong Zhou ),( Wei Li ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.1

Hepatitis B virus (HBV) genome P-encoded protein HBV DNA polymerase (Pol) has long been known as a reverse transcriptase during HBV replication. In this study, we investigated the impact of HBV Pol on host cellular processes, mainly apoptosis, and the underlying mechanisms. We showed a marked reduction in apoptotic rates in the HBV Pol-expressed HepG2 cells compared to controls. Moreover, a series of assays, i.e., yeast two-hybrid, GST pull-down, co-immunoprecipitation, and confocal laser scanning microscopy, identified the host factor eEF1A2 to be associated with HBV Pol. Furthermore, knockdown of eEF1A2 gene by siRNA abrogated the HBV Pol-mediated anti-apoptotic effect with apoptosis induced by endoplasmatic reticulum (ER) stress-inducer thapsigargin (TG), thus suggesting that the host factor eEF1A2 is essential for HBV Pol’s anti-apoptosis properties. Our findings have revealed a novel role for HBV Pol in its modulation of apoptosis through integrating with eEF1A2.

A new phenylethanoid glycoside with antioxidant and anti-HBV activity from Tarphochlamys affinis

Zhou, Xian-Li,Wen, Qing-Wei,Lin, Xing,Zhang, Shi-Jun,Li, Ying-Xin,Guo, You-Jia,Huang, Ren-Bin 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.5

A new phenylethanoid glycoside, named taraffinisoside A (1), together with five known glycosides were isolated from the stems and leaves of Tarphochlamys affinis. The structure of taraffinisoside A was identified on the basis of detailed spectral analysis. Compounds 1-4 and 6 showed potent antioxidant activities with $IC_{50}$ values of 10.36, 19.73, 43.95, 15.30 and $46.04{\mu}M$ by 1,1-diphenyl-2-picryhydrazyl radical-scavenging assay. Compounds 1, 2 and 4 showed anti-HBV activities, with $IC_{50}$ values of 0.50, 0.72 and 0.26 mM for HBsAg and 0.93, 0.42 and 0.07 mM for HBeAg, respectively.

A new phenylethanoid glycoside with antioxidant and anti-HBV activity from Tarphochlamys affinis

Xian-Li Zhou,Qing-Wei Wen,Xing Lin,Shi-Jun Zhang,Ying-Xin Li,You-Jia Guo,Ren Bin Huang 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.5

A new phenylethanoid glycoside, named taraffinisosideA (1), together with five known glycosideswere isolated from the stems and leaves of Tarphochlamysaffinis. The structure of taraffinisoside A was identified onthe basis of detailed spectral analysis. Compounds 1–4 and6 showed potent antioxidant activities with IC50 values of10.36, 19.73, 43.95, 15.30 and 46.04 lM by 1,1-diphenyl-2-picryhydrazyl radical-scavenging assay. Compounds 1, 2and 4 showed anti-HBV activities, with IC50 values of0.50, 0.72 and 0.26 mM for HBsAg and 0.93, 0.42 and0.07 mM for HBeAg, respectively.

Pristimerin Inhibits Breast Cancer Cell Migration by Up-regulating Regulator of G Protein Signaling 4 Expression

Mu, Xian-Min,Shi, Wei,Sun, Li-Xin,Li, Han,Wang, Yu-Rong,Jiang, Zhen-Zhou,Zhang, Lu-Yong Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Background/Aim: Pristimerin isolated from Celastrus and Maytenus spp can inhibit proteasome activity. However, whether pristimerin can modulate cancer metastasis is unknown. Methods: The impacts of pristimerin on the purified and intracellular chymotrypsin proteasomal activity, the levels of regulator of G protein signaling 4 (RGS 4) expression and breast cancer cell lamellipodia formation, and the migration and invasion were determined by enzymatic, Western blot, immunofluorescent, and transwell assays, respectively. Results: We found that pristimerin inhibited human chymotrypsin proteasomal activity in MDA-MB-231 cells in a dose-dependent manner. Pristimerin also inhibited breast cancer cell lamellipodia formation, migration, and invasion in vitro by up-regulating RGS4 expression. Thus, knockdown of RGS4 attenuated pristimerin-mediated inhibition of breast cancer cell migration and invasion. Furthermore, pristimerin inhibited growth and invasion of implanted breast tumors in mice. Conclusion: Pristmerin inhibits proteasomal activity and increases the levels of RGS4, inhibiting the migration and invasion of breast cancer cells.

Experimental Investigation on Fatigue Behavior of Steel Reinforced Concrete Composite Beam-to-Girder Joints

Le-Wei Tong,Qingjun Xian,Liying Zhou,Yiyi Chen,Yunfeng Zhang 한국강구조학회 2012 International Journal of Steel Structures Vol.12 No.4

Fatigue behavior and failure mechanism of steel reinforced concrete (SRC) beam-to-girder joints is discussed in this paper,which is intended for use in high-speed railway station structures due to their high stiffness and load capacity. Three identical SRC beam-to-girder joint specimens were designed and tested under static loading and two stages of fatigue loading. In the first stage of fatigue loading, the specimens were subjected to design fatigue load for 2 million cycles, while during the second stage, the specimens were loaded to failure under increased fatigue loading amplitude in order to know its fatigue strength and failure mechanism. The constructional details of SRC beam-to-girder joint specimen and the method of loading and testing are presented. The comparison in structural behavior of the joint is made between under static and fatigue loading. Fatigue failure characteristics of the joint are described in detail. It is found that the SRC beam-to-girder joints remained in their elastic range and the concrete surface crack did not exceed 0.1 mm when subjected to design static loading and 2 million cycles of design fatigue loading. There was no significant difference in structural behavior of each component of SRC composite beam between static and fatigue loading. Fatigue failure occurred after these joints were applied higher-level fatigue loading for another 0.70to 0.91 million cycles. Fatigue crack was initiated at the tension flange of I-shape steel of beam connected by welding to the flange of I-shape steel of girder or at the hole in tension flange of I-shape steel of beam, and then the crack propagated along flange width and web height of the I-shape steel in beam until the I-shape steel lost loading capacity due to lack of enough cross section. The fatigue behavior of constructional detail of the I-shape steel played a key role in the fatigue strength of the SRC beam-to-girder joints. Discussions on improving the fatigue strength of SRC beam-to-girder joints and future research aspects are presented finally.

Syntheses, Structure, and Properties of Four Metal-Organic Polymers Based on Rigid Multiple Carboxylate Ligands and N-Donor Ligands

Yong Zhou,Wen-Hao Zhao,Jin Wang,Jin-Rui Wei,Xian-Hong Yin,Xiang-Bo Wei,Cui-Wu Lin 대한화학회 2015 Bulletin of the Korean Chemical Society Vol.36 No.11

Four metal-organic coordination polymers, {[Cd2(BIBP)2(H2DTDA)2]n  · 2(H2O)} (1), [Zn (BIBP) (H2TDA)2] n (2), [Cd2(BIBP)(H2TDA)2] n (3), and [Ni2(BIBP)2(H2DTDA)2(μ-O)] n , (4) [where BIBP = 4,4′-bis(1-imidazoly)biphenyl, H2TDA = [1,1′:4′1″-terphen-yl]-3,3″-dicarboxylic acid, and H2DTDA = 2′,5′-dimethyl-[1,1′:4′,1″-terphenyl]-3,3″-dicarboxylic acid] were hydrothermally synthesized and characterized by elemental analyses, IR, TG, luminescence, and single crystal X-ray diffraction. X-Ray diffraction analysis reveals that the four complexes exhibit new frameworks due to diverse coordination conformations. The different coordination modes of the ligands BIBP and two aromatic carboxylate acids play important roles in the construction of the final structure for the complexes. X-ray structure analysis reveals that 1, 2, and 4 are 3D coordination polymers, while complex 3 is a 2D network polymer.

Experimental Investigation on Fatigue Strength of Joints between SRC Beams and Concrete-Filled RHS Columns

Qingjun Xian,Le-Wei Tong,Liying Zhou,Yiyi Chen 대한토목학회 2017 KSCE JOURNAL OF CIVIL ENGINEERING Vol.21 No.5

Fatigue behavior, failure mechanism and fatigue strength of joints between steel reinforced concrete (SRC) beams and Concrete Filled Rectangular Hollow Section (CFRHS) columns is discussed in this paper. Three identical beam-to-column joint specimens were designed and tested under static loading and two stages of fatigue loading. In the first stage of fatigue loading, the specimens were subjected to design fatigue load for 2 million cycles, while during the second stage, they were loaded to failure under increased fatigue load in order to know failure mechanism and fatigue strength. It is found that the joints satisfied design requirements when subjected to static loading and design fatigue loading. Fatigue failure occurred after these joints were applied higher-level fatigue loading. The crack initiated at the weld toe of stud or stirrup hole in the upper flange of I-shaped steel in certain SRC beam, and then it propagated along flange width in winding trajectory until fatigue fracture occurred. Stress amplitude of tension flange in SRC beam can be regarded as the parameter representing fatigue strength of the joints. S-N curves in related codes are selected to evaluate fatigue strength of the joints. The design method is suggested to consider fatigue design of the joints.

Anisotropic Patterns of Liver Cancer Prevalence in Guangxi in Southwest China: Is Local Climate a Contributing Factor?

Deng, Wei,Long, Long,Tang, Xian-Yan,Huang, Tian-Ren,Li, Ji-Lin,Rong, Min-Hua,Li, Ke-Zhi,Liu, Hai-Zhou Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.8

Geographic information system (GIS) technology has useful applications for epidemiology, enabling the detection of spatial patterns of disease dispersion and locating geographic areas at increased risk. In this study, we applied GIS technology to characterize the spatial pattern of mortality due to liver cancer in the autonomous region of Guangxi Zhuang in southwest China. A database with liver cancer mortality data for 1971-1973, 1990-1992, and 2004-2005, including geographic locations and climate conditions, was constructed, and the appropriate associations were investigated. It was found that the regions with the highest mortality rates were central Guangxi with Guigang City at the center, and southwest Guangxi centered in Fusui County. Regions with the lowest mortality rates were eastern Guangxi with Pingnan County at the center, and northern Guangxi centered in Sanjiang and Rongshui counties. Regarding climate conditions, in the 1990s the mortality rate of liver cancer positively correlated with average temperature and average minimum temperature, and negatively correlated with average precipitation. In 2004 through 2005, mortality due to liver cancer positively correlated with the average minimum temperature. Regions of high mortality had lower average humidity and higher average barometric pressure than did regions of low mortality. Our results provide information to benefit development of a regional liver cancer prevention program in Guangxi, and provide important information and a reference for exploring causes of liver cancer.

제11회 한,중 형법 국제 학술심포지엄 : 중국회사범죄 입법 중 자격형의 보완을 논함

천져씨옌 ( Ze Xian Chen ),쩌우웨이밍 ( Wei Ming Zhou ) 한국비교형사법학회 2013 비교형사법연구 Vol.15 No.2

중국의 1997년 형법에서는 회사가 형사책임의 주체가 되는 것을 인정하였고, 회사범죄에 대해 쌍벌제를 확립하였으나 회사의 재범능력을 제한시키거나 박탈하는 자격형(資格刑)과 관련해서는 아직 보완해야 할 부분이 굉장히 많다. 본문에서는 중국의 1997년 형법에서 기업범죄 입법내용과 방식에 대한 연구를 토대로 자격형에 있어서 결점과 부족한 부분이 있음을 지적하고, 기업범죄에 새로운 자격형을 신설하는 것에 대한 합리성을 논증함과 동시에 대륙법계국가 중 특히 프랑스의 입법경험을 참고하여 기업범죄 중 형사책임과 관련된 규정을 보완하고자 하였다. Although the company has been recognized as the subject of criminal responsibility and the bipartite punishment system against corporate crimes has been established in`s new penal code, the are still far from perfect. This article, with the analysis of the contents and the mode of the corporate crimes’ regulations in China`s new penal code, points out the defects and deficiencies in the regulations of qualification penalty; and by demonstrating the reasonableness of setting new qualification penalties against the corporate crimes, suggests that we should learn the legislative experiences of civil law countries and further improve the regulations of corporates` criminal responsibility.

Multiscale self-coordination of bidimensional empirical mode decomposition in image fusion

( Feng-ping An ),( Xian-wei Zhou ),( Da-chao Lin ) 한국인터넷정보학회 2015 KSII Transactions on Internet and Information Syst Vol.9 No.4

The bidimensional empirical mode decomposition (BEMD) algorithm with high adaptability is more suitable to process multiple image fusion than traditional image fusion. However, the advantages of this algorithm are limited by the end effects problem, multiscale integration problem and number difference of intrinsic mode functions in multiple images decomposition. This study proposes the multiscale self-coordination BEMD algorithm to solve this problem. This algorithm outside extending the feather information with the support vector machine which has a high degree of generalization, then it also overcomes the BEMD end effects problem with conventional mirror extension methods of data processing, . The coordination of the extreme value point of the source image helps solve the problem of multiscale information fusion. Results show that the proposed method is better than the wavelet and NSCT method in retaining the characteristics of the source image information and the details of the mutation information inherited from the source image and in significantly improving the signal-to-noise ratio.