RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향

박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.

덕유산 국립공원 등산로의 환경훼손에 대한 이용영향

서병수(Byung Soo Seo),김세천(Sei Cheon Kim),박종민(Chong Min Park),이창헌(Chang Heon Lee),이규완(Kyu Wan Lee) 한국산림과학회 1994 한국산림과학회지 Vol.83 No.3

The object of this study was to examine the user`s impacts on the environmental deteriorations of trail at Ticket Office - Paekryunsa (Temple) Hyangcho^kpong - Dongyupryung - Chilyun Fall area in To^kyusan National Park. Four trails were sampled in the study area according to the amount of users. Then the user`s impacts on trail were measured at each trail. The Ticket Office-Paekryunsa trail was the mo;;t used district and followed at Paekryunsa-Hyangcho^kpong trail, Hyangcho^kpong-Dongyupryung trail in descending order. Dongyupryung-Chilyun Fall trail is not used by people because of rest rotation system. The entire width of trail was greater at the more heavily used trail. Maximum depth, cross-sectional area loss, and surface texture and roughness of trail were the highest at Paekryunsa-Hyangcho^kpong trail. Soil hardness, soil acidity, soil moisture content, organic matter content, and exchange canon were influenced by trampling. Soil hardness, soil acidity and exchange canon increased in tramples soil, but content of soil moisture and organic matter decreased therein. Environmental deteriorations of trail were significantly influenced by the amount of users and the slope of trail. Bared lands about 2.000㎡ were appeared by trampling and camping around Hyangcho^kpong. Effects of carrying of rest rotation system for National Park were partly recognized at Dongyupryung-Chilyun Fall trail.

생리식염수 전기분해에 의한 세균 오염된 콘택트렌즈의 살균

김완석, 박준오, 최영기, 이영민, 김형규, 차은종, 김석용 충북대학교 동물의학연구소 2007 Journal of Biomedical and Translational Research Vol.8 No.1

본문참고

방송광고 요금과 판매방식에 관한 연구 : 우리나라와 주요 5개국의 비교를 중심으로

박원기,이규완 한국방송광고공사 2008 광고연구 Vol.0 No.78

The main purpose of this article is to make recommendations pertaining to the rate of broadcasting advertising and its method of sales in Korea by making comparisons with 5 leading advertising markets including USA, UK, Germany, France and Japan. With respect to the rate of broadcasting advertising, the overall tendency of‘ price range’is shifting from a cost-centered approach to a greater focus on media value, while‘ price application’is moving from a single unit structure to a multi-unit structure. On the advertising method of sales, it is found that the various packaged method of sales, the controversial issue today, are commonly used in many countries other than in Korea. Since the matter of subjectivity in determining advertising rates cannot altogether be avoided, it is necessary that relevant parties make continuous efforts to improve the current system. On the lack of flexibility of method of sales and related matters, as it is evident alterations to the current method of sales are necessary, some improvements are expected in the near future. 본 논문의 주된 연구목적은 광고비 지출 면에서 상위 5개국인 미국, 영국, 독일, 프랑스, 일본 등의 방송광고 요금과 판매방식을 우리나라와 비교하여 그 현황을 살펴보고 개선방안을 제시하는 데 있다. 방송광고 요금 측면에서는 국가별 차이에도 불구하고 전체적 흐름을 보면‘가격범위’면에서는 원가 중심에서 매체가치의 비중을 높이는 방향으로 움직이고, ‘가격적용’면에서는 단일요금제에서 차등요금제로 움직이고 있다고 할 수 있다. 방송광고 판매방식과 관련하여 쟁점이 되고 있는 패키지 판매의 경우 우리나라에만 존재하는 방식이 아님을 확인할 수 있었다. 요금 책정의 객관성 문제는 현실적으로 불가피한 면이 있기 때문에 향후 당사자 간의 지속적인 개선 노력이 필요하다. 판매방식의 경직성 문제 등에 대해서는 개선이 필요하다.

강원대학교 연습림내 蓮葉山과 九節山地域의 植物相에 관한 硏究

朴完根,廉圭眞,朴善貞 江原大學校 森林科學硏究所 1997 Journal of Forest Science Vol.13 No.-

要約강원대학교 연습림의 연엽산과 구절산 지역을 대상으로 植物相을 조사한 결과, 管束植物資源은 98科 345屬 551種 2亞種 88變種 10品種 2雜種으로 총 653분류군이 분포하는 것으로 나타났다. 이들 所産植物중 韓國特産植物은 20種類, 그리고 稀貴 및 滅種危機植物은 18種類가 분포하였으며, 自然生態系에 예측할 수 없는 영향을 초래하는 歸化植物은 27種類가 출현하였다. 귀화식물 種類수에 의한 自然破壞度(UI)는 약 12.4%로 나타났다. 또한 所産植物들의 有用度는 食用資源(42.1%), 藥用資源(33.8%), 牧草用(28.8%), 觀賞資源(11.8%), 木材資源(5.2%), 纖維資源(1.4%), 工業用(1.1%) 등의 순으로 나타났으며, 아직 용도가 밝혀지지 않은 植物資源도 140種類(21.4%)로 나타났다.ABSTRACTThis study was to survey the flora of resources vascular plants in the Experiment Forests of Kangwon National University. Resources vascular plants were composed of 98 families, 345 genera, 551 species, 2 subspecies, 88 varieties, 10 formae, and 2 hybrid, totaling 653 taxa. Among the investigated resources plants, Korean endemic plants and rare or endangered plants were distributed 20 taxa and 18 taxa, respectively. There were 27 taxa of Naturalized plants in this study area, which would have unpredictable influence on the natural ecosystem. The urbanized index(UI) of all over the investigated areas was about 12.4%. Useful resources plants were composed edible source(42.1%), medicinal source(33.8%), pasture source(28.8%), ornamental source(11.8%), timber source(5.2%), fiber source(1.4%), industrial source(1.1%). Vascular plants of unknown use were 140 taxa(21.4%)

TMS320F240을 이용한 PWM 인버터 유도전동기 구동 시스템의 전도노이즈 저감을 위한 스위칭 기법

朴奎炫,金利勳,元忠淵,金奎植,崔世琓,咸年根 성균관대학교 2003 학술회의지원논문목록집 Vol.2003 No.-

PWM 인버터에 의해 구동되는 유도전동기에 발생되는 고주파 커먼 모드 전압은 전도성 EMI, 전동기 접지전류, 베어링 전류 및 다른 원치 않는 결과의 주원인이 된다. 인버터 제어의 무효벡터 스위칭 상태는 나머지 유효벡터 스위칭 상태에 비교하여 큰 커먼 모드 전압을 일으킨다. 그러므로 이 논문은 커먼 모드 전압을 완화하는 스위칭기법을 다룬다. 즉 정현파 PWM기법을 기본으로 한 커먼 모드 전압 제거방법을 제안하였다. PWM 신호는 각각의 정현파 기준신호와 120˚위상차가 나는 3개의 캐리어 파형과 비교하여 발생된다. 시뮬레이션과 실험적결과는 제안된 PWM 기법에서 커먼 모드 전압이 종래의 PWM 기법보다 약 66% 더 저감됨을 보였다. High frequency common mode voltage produced by PWM inverter fed induction motor is a major cause of conducted EMI. creation motor ground currents, bearing currents and other hamful products. The zero switching states of inverter control invoke large in comparison with the non-zero switching state of inverter control. We proposed a common mode voltage reduction method based on sinusoidal PWM technique. PWM signal are generated by comparing respective sinusoidal reference signal with three triangular carrier wave displaced of 120˚. Simulation and experimental result show that common mode voltages in the propsed PWM technique are reduced by approximate 66% more than conventional PWM technique.

위암세포에 의한 종양침윤 림프구의 면역반응 억제기전에 관한 연구

박정규,송규상,서광선,최정목,배진선,장일성,윤완희,노승무,조은경,백태현 大韓免疫學會 1995 大韓免疫學會誌 Vol.17 No.3

Tumor-infiltrating lymphocytes ('1°ILs) interact most closely with tumor cells and thus are more likely to reflect tumor host interactions accurately. But it is unknown whether such T cells are nonspecific inflammatory cells or a subset of specific host immune responses. In this study, there was no clear correlation between the infiltration of T lymphocytes in stomach cancer and the overexpression of c-ErbB-2 or increasing class I MHC expression on tumor cells. A positive correlation was seen between the presence of TILs in the tumor and tumors with diploidy by flow cytometric DNA analysis. The proliferative responses of Ills stimulated with IL-2, anti-CD3 mAb, or both were examined. When compared to normal mucosal-associated lymphoid tissue lymphocytes, the proliferative response of TILs to high dose IL-2 was minimal. A similarly poor response to anti-CD3 mAb plus IL-2 was also observed. The freshly isolated TILs exhibit reduced ability to proliferate in response to IL-2, anti-CD3 mAb or both. The microenvironment of the tumor suppresses the proliferative capacity of the TILs. The mechanism of this suppression remains unknown. It could be mediated by suppressor cells, by soluble substances within the tumor, or both. To examine this question, supernatants of stomach cancer cells (SNSNU-1) were tested for the presence of immunosuppressive factors. Human peripheral blood T-cells and tumor-draining lymph node lymphocytes (TDLNL) were incubated for 3 days with SNSNU-1 and then assessed for proliferative responses to PMA, anti-CD28 mAb, or both and for the inducibility to express IFN- r or IL-4 mRNA to PMA. Peripheral blood T-cells pretreated with SNSNU-1 were unable to proliferate in response to PMA, anti-CD28 mAb or both. SNSNU-1 also produces inhibitory activities of TDLNL proliferative response to PMA or anti-CD28 mAb and PMA (49%, 52%, respectively). In contrast, culture supernatants obtained from HEp-2, K562 or Daudi showed normal proliferative responsiveness of peripheral blood T-cells and TDLNL by PMA, anti-CD28 mAb or both.

內部整列 알고리즘에 關한 分析 및 應用

朴淳圭,金完奎,金昌根 진주산업대학교 농업기술연구소 1990 農業技術硏究所報 Vol.3 No.-

내부 정렬에 관한 알고리즘들을 분석하고 PASCAL로 응용 프로그램화 하여 각 알고리즘들의 정렬 속도 및 효율성을 실험적 방법으로 비교 평가 하였다. 각 알고리즘에 대한 응용 프로그램의 수행결과를 요약하면 다음과 같다. 1. Straight Insertion sort는 초기상태의 입력자료가 순서배열 되어있는 경우와 소량의 데이터 화일에서 적용이 바람직하다. 2. Bubblesort는 실행시간이 가장 늦었으면 내부정렬 방법들 중에서 가장 비효율적이다. 3. Quicksort는 실행시간이 빨랐으며 내부정렬 방법들 중에서 효율이 가장 우수하다. 4. Heapsort는 실행 시간 면에서는 상당히 빠르지만 입력화일을 Tree로 구성하고 Heap성질이 유지되도록 재 조정이 필요하다. 5. Two-way Mergesort는 레코드의 길이가 길 경우에 레코드들의 이동에 걸리는 시간이 실행시간에 큰영향을 준다. The objects of this study is to investigate experimentely the sorting time and efficiency of internal sort algorithms. To generate sample data, We used the system's random-number generator. The experiments included both large and small samples of data with numeric keys. Table1 shows the sorting times(in milliseconds)consumed by the application programs of sort algorithms. as executed by the Turbo-Pascal system on a PRO-3000 computer. The results of experiments are as follows: 1. Bubblesort is definitely the wrost sorting method among all compared. 2. Quicksort has even strengthened its position as the quickest method and appears as the best array sorter by far.

원심모형시험을 이용한 모래다짐말뚝 시공지반의 호안제체 사면안정에 관한 연구

朴鎔遠,柳完圭,白寅哲,金濟奎 명지대학교 산업기술연구소 2008 産業技術硏究所論文集 Vol.27 No.-

Centrifuge modelling tests with embankment model on sand-clay composite soils(a type of soil which sand compaction piles are established) at the 15.5 and 20% replacement ratio(a_(s)) and with/without geotextile were performed to comprehend the effect of a_(s), and geotextile on slope stability of embankment From the test results, sand-clay composite soils at the 15.5 and 20% replacement ratio(a_(s)) are safety for slope failure(circular slip) and a geotextile reinforced model is better than a non-reinforced model on overall settlement and upheaval of ground surface in front of embankment.

Acetic Acid Alkylacetate계의 기액평형

박원규,심홍섭 영남이공대학 1979 論文集 Vol.8 No.-

Vapor-liquid equilibrium data for the binary systems of ethylacctate acetic acid, u-propyiacctate acetic acid, acetic acid nbutylacetate and acetic acid-iso amylacetate were investigated. It was shown that the logarithmic value of relative volatility (iogcr) exhibited a linear function of the liquid composition(x) icr systems. And these linear relationships for each of four systems indicate a strong tendency falling into a single point, when each line is extended. The present study proposed the empirical equations by which x-y data for. each binary system of acetic acid aPaylacetate can be estimated. And the x-y data estimated by the proposed equations are compared with the experimented data, and proved to be in a pied coincidence with them.