Application of Artificial Insemination Technology for Dairy Breeding in Mongolia

Hyun‐Tae Jo,Jong‐In Jin,Seong‐Su Kim,Byung‐Hyun Choi,Tumor Baldan,Jung‐Gyu Lee,Yun‐Shik Kim,Sam‐Churl Kim,Il‐Keun Kong 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1

This study was focused on improvement of milk production in Mongolia dairy industry by artificial insemination (AI) technology, of which was supported from ODA project of KOICA in Republic of Korea. The study was started from January 2009 to present and 3rd years in this year. So, all data, especially synchronization and pregnancy of dairy cows (Holstein) will be summarized in final result in this year. For synchronization, total 81 dairy cows selected from 4 private farms that were 38, 30, 8 and 5 in Undarmal milk, Onjin (Enkhbayer), Jargalant, and BRM School, respectively. All the dairy cows were injected intramuscular (IM) of 5 ml PGF2α in the vulva and detected estrus 2 to 3 days after PGF2α injection. Total 78 out of 81 dairy cows (96.3%) were detected estrus by only 1 time injection of PGF2α. The dairy cows that were induced estrus, inseminated with 0.5 ml dairy frozen semen by conventional AI techniques. The pregnancy diagnosis of the AI dairy cows was detected by uterus palpation after 60 days of insemination. Total 75 from 78 inseminated dairy cows (90.1%) were diagnosis pregnant. The estrus induction and pregnant rate were very effective using PGF2α injection and conventional AI techniques in Mongolia dairy cow. The results indicated that AI after estrus induction in Mongolia dairy cows could be applied to dairy breeding technology to improve the breeding efficiency and milk production.

21가지 장미꽃 품종의 페놀성 화합물 함량 및 항산화 활성

조연재(Yeon Jae Jo),서정현(Jeong Hyun Seo),홍채영(Chae Young Hong),김성태(Seung Tae Kim),최은경(Ehn-Kyoung Choi),김윤배(Yun-Bae Kim),이준수(Junsoo Lee),정헌상(Heon Sang Jeong) 한국식품영양과학회 2021 한국식품영양과학회지 Vol.50 No.4

본 연구는 21품종의 장미꽃에 대한 식품 및 화장품 원료로 사용 가능성을 살펴보기 위해 페놀성 화합물과 항산화 활성을 평가하였다. 총 폴리페놀, 총 플라보노이드 및 타닌 함량은 러버샤이 품종에서 각각 379.70 mg GAE/extract g, 50.10 mg CE/extract g 및 339.44 mg TAE/extract g으로 높게 나타났으며, 칠백리 품종에서 각각 107.25 mg GAE/extract g, 9.46 mg CE/extract g 및 36.31 mg TAE/extract g으로 낮았다. 페놀산 함량은 총 폴리페놀 함량과 유사한 결과를 보였으며, 러버샤이 품종에서 27.40 mg/extract g으로 높았다. 안토시아닌은 대부분 cyanidin-3,5-diglucoside로 확인되었으며 총 안토시아닌 함량은 유니나 품종에서 8.80 mg/extract g으로 가장 높았다. ABTS 및 DPPH 라디칼 소거 활성은 각각 428.10 및 419.59 mg AAE/extract g로 러버샤이 품종에서 가장 높았으며, 항산화 활성과 페놀 화합물의 함량과 높은 양의 상관관계를 보였다. 이러한 결과는 러버샤이와 같은 항산화 활성이 뛰어난 품종들이 관상 목적뿐만 아니라 높은 기능성을 가진 식품 및 화장품의 원료로 사용 가능성이 있다고 판단된다. This study evaluated the functional components and antioxidant activities of 21 different rose flower extracts, to confirm their value as a food and cosmetics ingredient. The highest values of total polyphenol, flavonoid and tannin contents (379.70±1.64 mg GAE/extract g, 50.10±0.76 mg CE/extract g, and 339.44±1.20 mg TAE/extract g, respectively) were obtained in Lover Shy. The proanthocyanidin content ranged from 61.88 mg CE/extract g in the Unina cultivar to 5.34±0.13 mg CE/extract g in the Aileen cultivar. The phenolic acid content obtained was highest in the Lover Shy cultivar (27.40±0.26 mg/extract g), whereas highest levels of anthocyanin (8.83±0.03 mg/extract g) were obtained in the Unina cultivar. High ABTS and DPPH radical scavenging activities were determined in the Lover Shy cultivar (428.10±0.29 and 419.59±3.12 mg AAE/extract g, respectively). Taken together, these results indicate that the antioxidant activity and functional components of Lover Shy cultivar are superior, and this extract can potentially be applied as a food and cosmetic ingredient.

결핵균 단세포군항체에 의한 항산균항원의 면역화학적 분석에 관한 연구

최대경,백태현,박정규,김화중 충남대학교부설 생명공학연구소 1991 생물공학연구지 Vol.1 No.-

β-antigen is one of the major protein of Mycobacterium tuberculosis. We purified reference β-antigen from the unheated culture filtrate of Mycobacterium tuberculosis by serial ammonium sulfate precipitation, gel filtration on Sephacryl S-200 column and preparative polyacryamide gel electrophoresis. Nine monoclonal antibodies against β-antigen were produced by hybridoma technique. The nine monoclonal antibodies were divided into two groups according to their reaction patterns on Western blots. Four monoclonal antibodies(group Ⅰ) reacted with two major bands of 83 and 52 kDa, and two minor bands of 110 and 56 kDa molecules while the other five monoclonal antibodies(group Ⅱ) reacted with a major band of 83 kDa and a minor and of 110 kDa molecule. The interspecies reactivities of nine monoclonal antibodies on the sonicated antigens from 21 species of mycobacteria were also examined by dot blots. Two monoclonal antibodies, designated 4G5E10(group Ⅰ) and 5F3F2(group Ⅱ), showed a specificity restricted to Mycobacterium tuberculosis complex. β-antigien from the cell extract of Mycobacterium tuberculosis was purified by affinity chromatography with 5F3F2 monoclonal antibody and checked its molecular characterization. Affinity purified β-antigen showed two distinct bands of 83 and 52 kDa molecules on SDS-PAGE. These results indicating that β-antigen is a associated protein with molecular weights of 83 and 52 kDa, and contains at least two different specific epitopes for Mycobacterium tuberculosis complex on β- antigen molecules. Affinity purified and reference β- antigens were applied for its potential diagnostic utility for determination of LgG antibody in the cerebrospinal fluids and sera of tuberculosis patients by ELISA. The mean ELISA values of IgG antibody activities to affinity purified β-antigen at the CSF dilution of 1 : 5 were 0.866±0.607 in 12 patients with tuberculous meningitis and 0.058±0.040 in 24 controls(P<0.001). The mean ELISA values of IgG antibody activities to reference β-antigen at the serum dilution of 1 : 100 were 0.861±0.350 in 97 patients with minimal tuberculosis, 1.052±0.443 in 51 patients with moderately advanced, 1.381±0.396 in 49 patients with far advanced and 0.454±0.248 in 138 controls(P<0.01). An indirect sandwich ELISA using specific biotinylated 5F3F2 monoclonal antibody as tracer was designed to detect β-antigen in the culture filtrate of Mycobacterium tuberculosis and sputum samples from patients with pulmonary tuberculosis. The detection limit of indirect sandwich ELISA was 0.1㎍ culture filtrate/㎖. The mean ELISA values of β-antigen reactivities in sputum samples from 52 patients with pulmonary tuberculosis and 25 controls were 0.766±0.404 and 0.453±0.133(not significant).

공공체육시설의 활용방안

최종환,김창범,이규문,김태완,김현준,박종진 忠北大學校 平生體育硏究所 1995 平生體育硏究所 論文集 Vol.7 No.-

An effective use of public facilities for life sports was studied. The purpose of this study was 1) to examine the present management and sport programs of the public sport facilities, and 20 to provide more effective and productive sports management and programs for Cheongju-city community, which will be resulting in an independence of Chungbuk Athletics Association and the enhancement of level of citizens' health and physical fitness. After the investigation on the present situations of managing public sport facilities, this study showed the following results and suggestions. First, this study found several problems (e.g, a few numbers of sport experts and programs, ineffective opening and closing time for each facility, etc..) and suggested new plans on the management of public facilities for sport activities. Second, this study provided more practical sport programs for each age group, regarding to each public sport facility. In addition, these sport facilities were also used for cultural activities and resting places for the community. Third, this study also suggested that an indepence of Chungbuk Athletic Association and the enhancement of level of citizens' health and physical fitness should be consequently accomplished by activation of effective management on sport programs and facilities.

1, 2 차원 수치해석에 따른 기존 세굴심 산정식 편차 산정

최한규,박태현,이영섭 江原大學校 産業技術硏究所 2008 産業技術硏究 Vol.28 No.B

This study tried the 1st, 2nd dimensional numerical analysis according to the pier's shape, size and installing method in order to compare the depth of scour calculation method using the variables calculated by using the 2nd dimensional numerical analysis with the calculated depth of scour value by using the calculated variables by using the 1st dimensional numerical analysis. And then verified the problems occurring when the depth of scour is calculated by using the calculated values by using the 1st dimensional numerical analysis, as calculating the deviation depending on it.

SmartDoumi: 서비스 로봇의 하이브리드 제어구조

최경현,박태호 제주대학교 산업기술연구소 2000 尖端技術硏究所論文集 Vol.11 No.2

This paper addresses a hybrid control architecture for the hospital service robot. Smart Worker. In hybrid architecture, the deliberation takes place at planning layer while the reaction is dealt through the parallel execution of operations. Hence, the system presents both a hierarchical and an heterarchical decomposition, being able to show a predictable response while keeping rapid reactivity to the dynamic environment. In order to model the control behavior of this controller, this paper also proposes MuLOM(Muti-Layered Operation Model). Moreover, through simulation under a virtual environment IGRIP, the effectiveness of hybrid architecture and MuLOM is verified.

Hirschsprung 씨 질환에서 항문직장계측검사의 진단적 의의

최순옥,강중신,백태원,박우현 啓明大學校 醫科大學 1987 계명의대학술지 Vol.6 No.2

It is well known that stimulation of rectum by balloon distention results in the reflex relaxation of the internal anal sphincter in the normally innervated bowel. and this rectosphincteric reflex is absent in Hirschsprung's disease. It's the first report in Korea to evaluate diagnostic reliability of anorectal pressure study in Hirschsprung's disease using anorectal manometry, Model 003 developed by professor Holschneider. This investigation was performed on 21 consecutive children who complained of acute intestinal obstruction especially in infants and chronic constipation during the period February 1986 to September 1987. The ages of the children ranged from 8 days to 15 years, and 8 patients including the 4 neonates were examined within 1 year old. The 7 patients were proved to have Hirschsprung's disease who revealed no rectosphincteric reflex on anorectal pressure study, and Hirschsprung's disease was ruled out in 14 patients who reveales rectosphincteric reflex. In 2 out of 14 patients who showed no rectosphincteric reflex, Hirschsprung's disease was ruled out by rectal suction biopsy and clinical course. With the above results, this study shows an over-all diagnostic accuracy 90% along with sensitivity 100% and specificity 86%. In conclusion, anorectal pressure study is a reliable, diagnostic procedure for Hirschsprung's disease, and it has the advantage of simple , non-invasive and safe procedure without general anesthesia and is of particular value as a screening test in inpatients as well as outpatients.

펜싱 종목별 연습 및 시합시 심박수 변화

최태석,이근배,천영호,이현수 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.4

This experiment was done on 30 fencers that had joined the national competition and had a research on theft heart rates during the fencing bout and got the following results. 1. In average, a male fencer have a height of 178.40㎝, weight of 72.50㎏, the height when sitting of 93.70㎝, the chest perimeter of 94.70㎝, arm length of 56.50㎝, and thigh parameter of 60.20㎝. In average, for a female fencer, had a height of 167.98㎝, weight of 59.00㎏, the height when sitting of 87.00㎝, chest perimeter of 84.50㎝, the thigh perimeter of 58.30㎝, and the arm length of 50.90㎝. From the result of testing a fencer's endurance by general footwork, it was proofed that the evaluation of respiratory function was needed more. 3. When comparing the heart rates of male fencers in the fencing bout of fleuret and epee, we found out that the fleuret fencer had a higher heart rate, in average of 17.06beat/min, than the epee fencer. However, there was not much difference with the heart rates during the bouts of sabre and fleuret Clearly, the bout during epee showed lowest heart rate among these three subjects. 4. The heart rates during female's fencing bout, there was not much difference with the heart rates in the male's fencing bout, and the heart rate during a bout of epee was about 18.59beat/min lower than fleuret.

p-형 Si(100) 기판 위에 성장시킨 Bi_(3.25)La_(0.75)Ti₃O_(12) (BLT) 박막의 전기적 특성

최은경,박문흠,김현주,하태곤,강민주,김상수 昌原大學校 基礎科學硏究所 2003 基礎科學硏究所論文集 Vol.15 No.-

Bi₃zsLa(0.75)Ti₃0_(12) (BLT) thin film have been grown on p-type Si(l00) substrates by a sol-gel spin coating process. The structure and surface morphology of the film were analyzed by X-ray diffraction (XRD) and scanning electron microscopy (SEMI. Furthermore, the electrical properties were examined by capacitance-voltage ( C-V) characteristics by using a LF Impedance Analyzer(HP 4192A). XRD measurement show layered perovskite structure with a single phase in BLT thin film annealed at 700C for 30 min in oxygen. The measured grain size of BLT on Si is about 0.04 - 0.1 pn. From the cross-sectional SEM image, BLT film thickness is measured to be 0.4 pm. The C-V characteristic hysteresis curves show that the NIFS (metal-fexroelectric-semiconductor) structure has memory effect. The memory window decreases, with increasing frequency at MFS structure. And it is found that the memory window increases as the applied voltage to the ferroelectric capacitor is increased. These phenomena may be due to polarization reversals of the BLT ferroelectric film and because of the charge injection from Si substrate into the ferroelectric thin film. The results obtained indicate that the present BLT films are suitable for making ferroelectric field effect transistor(FeFET) memory.

초음파로 진단된 자궁동정맥 기형 2예

최홍준,이해혁,김태희,김정식,남계현,이권해,박성진 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Arteriovenous fistula of uterus is a very rare cause of massive uterine bleeding. However, it is important to make a rapid and precise diagnosis when it does occur, because life-threatening massive vaginal bleeding may occasionally be a symptom of this disease. Uterine curettage or surgical trauma can cause vascular abnormalities, including pseudoaneurysms, acquired ateriovenous malformations, ateriovenous fistulas, and rupture of vessels. The diagnosis is made by angiography, and gray scale ultrasonography, color and duplex doppler ultrasonography, computed tomography, magnetic resonance imaging is helpful. Ultrasonography is the most commonly performed initial imaging examination for evaluation of abnormal uterine bleeding. Color and duplex Doppler US is an appropriate modality for the detection and diagnosis of uterine ateriovenous malformations and for follow-up after embolization. Transcatheter embolization has replaced hysterectomy as the treatment of choice in women wish to retain thier fertility. A case of arteriovenous malformation of the uterus which was diagnosed by ultrasonography. So reported this case with a brief review of the literature.