진공용 나노스테이지 개발

홍원표,강은구,이석우,최헌종 한국공작기계학회 2004 한국공작기계학회 춘계학술대회논문집 Vol.2004 No.-

Miniaturization is the central theme in modem fabrication technology. Many of the components used in modern products are becoming smaller and smaller. The direct write FIB technology has several advantages over contemporary micromachining technology, including better feature resolution with low lateral scattering and capability of maskless fabrication. Therefore, the application of focused ion beam(FIB) technology in micro fabricaton has beome increasingly popular. In recent model of FIB, however the feeding system has been a very coarse resolution of about a few μm. It is not unsuitable to the sputtering and the deposition to make the high-precision structure in micro or macro scale. Our research is the development of nano stage of 200mm strokes and 10nm resolutions. Also, this stage should be effectively operating in ultra high vacuum of about 1×10^(-5)pa. This paper presents the concept of nano stages and the discussion of the material treatment for ultra high vacuum.

스포츠 브랜드 이미지 척도 표준화에 관한 연구

홍석표 한국스포츠산업경영학회 2002 한국스포츠산업경영학회지 Vol.7 No.1

본 연구는 현재 소비자 행동론에서 가장 보편적으로 사용되어지는 브랜드 이미지 측정 도구 중하나인 Aaker(1997)의 척도를 국내 스포츠 상황에 적합한 표준화를 통한 그 사용 가능성을 알아보는데 그 목적이 있다. 본 연구의 조사대상은 한국 프로농구리그 관람자를 대상으로 경기장에서 직접 조사하였으며, 분석에 이용된 총 표본수는 209명이었다. 분석을 위하여 LISREL 8.5 프로그램을 이용한 확인적 요인분석을 사용하였다. 본 연구의 결과에 의하면, Asker의 15개의 하위요인 가운데 10개 요인이 한국 스포츠 현장에서 브랜드 이미지를 측정하는 데 유의한 요인인 것으로 나타났으며, 5개 하위영역 중 4개가 스포츠 브랜드 이미지를 측정 시 의미 있는 영역으로 나타났다. 또한, 각 요인 및 하위영역에 대한 신뢰도 및 타당도를 검증할 결과 모든 요인과 영역에서 비교적 높은 타당도와 신뢰도를 보여주고 있었다. 이에. 본 연구에서 표준화한 스포츠 브랜드 이미지 척도는 스포츠 국내 스포츠 현장에 그 활용성이 높은 것으로 나타났다. The purpose of this study was to standardize a brand image scale into sports contexts in Korea. To achieve the purpose of this study. Brand Personality Scale(BPS) developed by Aaker(1997) was used to translate and analyze its adaptability in Korean. The BPS was consisted of 15 facets with 5 sub-domains. A total of 209 sample who were spectatos in Korean Baseball League(KBL) was used for data analysis. Results indicated that 10 facets among original 15 original facets significantly represented brand image in sports contexts of Korean Baseball League. Also, four sub domain among original five were statistically fit with proposed model for Korean version of brand image scale. In detail, sincerity, excitement, competence, and ruggedness were shown as good sub domain for the scale. The result indicated that Korean version of sport brand image scale included relatively high reliability and validity.

PCR을 이용한 Rat 기관지 세척액에서의 Pneumocystis carinii DNA의 검출 : 기초실험 A pilot study

김주옥,홍석철,한표성,이종진,김선영,이영하,신대환 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.1

Background: Pneumocystis carinii (PC) is a opportunistic pathogen causing serious pneumonia (Pneumocystis carinii pneumonia : PCP) in immunosuppressed patients including AIDS. Laboratory diagnosis of PCP is dependent on microscopic demonstration of the PC by using cytochemical staining or by immunocytochemical staining with monoclonal antibodies. However, these staining methods are not highly sensitive. The development of the polymerase chain reaction (PCR) has made it possible to detect very small numbers of pathogens in clinical specimens. To establish the usefulness of PCR for detection of PC DNA, We performed PCR as a pilot study. Methods: PCP was induced on Spraque-Dawley rats with prednisolone 5mg IM twice a week. Bronchial lavages, impression smears, and permanent sections were performed from the 3rd week to 7th week of cortisonized rats. PC DNA was extracted with bead beater / 10% CTAB method. Results: Impression smears showed PC cyst after the 4th week (100%, 28/28 rats), and permanent sections showed 27/28 rats. Control rats showed some PC cysts (2/7). PCR result was positive only one case among the 6th week rats (1/7), But positive 6 cases at the 7th week rats, (6/7). Among control rats. 2 cases were positive (2/7). ??This data is a pilot study for the PC DNA detection using a PCR. PCP were successfully cortisonized rats. If adequate bronchial washing technique will be used, PCR can be a sensitive for PC DNA detection.

고속 HMC 이송계의 운동특성 평가 및 운동오차 예측

최헌종,허남환,강은구,이석우,홍원표 한국공작기계학회 2004 한국공작기계학회 추계학술대회논문집 Vol.2004 No.-

Recently, the evolution in production techniques (e.g. high-speed milling) and the complex shapes involved in modern production design has been increasingly popular. The key to the achievement is a drastic improvement of the dynamic behavior of the machine tool axes used in production machinery. The more complex these tool paths the higher the speed and acceleration requirements. But it is very difficult to reach the target for high speed machine tool because of the limitations of servo system and motion control system. However the direct drive design of machine tool axes, which is based on linear motors and which recently appeared on the market, is a viable candidate to meet the ever increasing demands, because of these advantages such as no backlash, less friction, more mechanical simplicity and very higher acceleration and velocity comparing to the traditional system. This paper focused on the performance tests of the high speed horizontal machine tool based on linear motor. Especially, dynamic characteristics were investigated through circular test and circular form machining test is carried out considering many important parameter. Therefore these several experiments is used to be evaluated the model for prediction of circular motion error and circular machined error.

FIB를 이용한 나노가공공정 기술 개발

최헌종,강은구,이석우,홍원표 한국공작기계학회 2004 한국공작기계학회 춘계학술대회논문집 Vol.2004 No.-

The application of focused ion beam (FIB) technology in micro/nano machining has become increasingly popular. Its use in micro/nano machining has advantages over contemporary photolithography or other micro/nano machining technologies, such as small feature resolution, the ability to process without masks and being accommodating for a variety of materials and geometries. This paper presents that the recent development and our research goals in FIB nano machining technology are given. The emphasis will be on direct milling, or chemical vapor deposition techniques (CVD), and this can distinguish the FIB technology from the contemporary photolithography process and provide a vital alternative to it. After an introduction to the technology and its FIB principles, the recent developments in using milling or deposition techniques for making various high-quality devices and high-precision components at the micro/nano meter scale are examined and discussed. Finally, conclusions are presented to summarize the recent work and to suggest the areas for improving the FIB milling technology and for studying our future research.

대퇴동맥을 통한 경피적 관동맥 중재시술 환자의 천자부위 지혈을 위한 Angioseal^(�) 사용과 고식적 용수 압박법의 비교 : 전향적 연구

김용훈,권현철,김필호,안석진,유철웅,최진호,이상철,김준수,김덕경,전은석,이상훈,홍경표,박정의,서정돈 대한내과학회 2004 대한내과학회지 Vol.66 No.5

목적 : 경피적 관동맥 중재술은 최근 양적 및 질적으로 급격한 발전을 보였지만 시술 시 천자부위의 혈관 합병증은 아직 해결해야 할 문제점이다. 저자들은 대퇴동맥을 통한 경피적 관동맥 중재술을 환자에게 천자부위 지혈을 위한 혈관폐쇄기구인 안지오실의 안전성과 유용성을 고식적인 용수 압박법과 비교 연구하고자 하였다. 방법 : 2002년 4월부터 2003년 5월 사이에 삼성서울병원 심장혈관센터에서 대퇴동맥을 통한 경피적 관동맥 중재술을 성공적으로 시행한 200명의 환자(안지오실 사용군: A군, 100명, 고식적 용수압박법 사용군 B군, 100명)를 대상으로 시술 후 주요 합병증 및 경한 합병증, 지혈 후 환자가 자리에 앉기까지의 시간, 보행개시 가능시간, 총 재원 기간과 시술 1주 후의 합병증을 전향적으로 조사하여 비교 연구하였다. 결론 : 두 군에서 연령, 성별, 기저질환, 심혈관 질환의 위험요소, 시술의 종류, 시술 중 사용한 헤파린의 양, clopidogrel의 양, ticlopidine의 양, 지혈 시 수축기와 확장기혈압, ACT (activated clotting time)는 차이가 없었다. A군이 B군에 비해 시술 후 자리에 앉기까지의 시간 (A군: 4.3±0.3시간, B군: 13.7±0.8시간, p=0.004) 및 보행개시까지의 시간(A군: 6.8±0.5시간, B군: 18.8±2.1시간, p=0.013)이 유의하게 짧았다. 시술 후 주요 합병증은 두군 모두에서 관찰되지 않았으며, 경한 합병증의 전체발생은 A군에서 유의하게 적었다(A군: 28명, B군: 19명, p=0.003). 반상출혈의 경우는 A군에서 유의하게 낮았지만(A군: 3명, B군: 12명, p=0.01), 혈종, 출혈의 발생은 두군간에 차이가 없었다. 총 재원기간에는 두 군간에 차이가 없었다(p=0.239). 시술 1주 후 경과관찰에서 주요합병증은 역시 두 군 모두에서 관찰되지 않았으며, 경한 합병증의 전체발생은 두 군에서 차이가 없었다(A군: 15명, B군: 13명 p=0.418), 반상출혈의 빈도는 차이가 없었지만 혈종의 발생은 A군에서 더 낮았다(A군: 2명, B군:6명, p=0.004). 두 군 모두에서 출혈은 발생하지 않았다. 결론 : 대퇴동맥을 통한 경피적 관동맥 중재술을 시행한 환자에서 안지오실의 사용은 고식적 용수 압박법에 비해 환자의 침상 안정시간을 줄여 조기 활동개시가 가능하게 하였으며 국소합병증의 위험도를 일부 낮추어, 시술에 따른 환자의 불편을 현저하게 감소시킬 수 있다고 할 수 있을 것으로 기대된다. Background : Although the number and the quality of percutaneous coronary intervention have been recently increased dramatically, the vascular complication at puncture site is still the major cause of patients' morbidity. We evaluated the safety and efficacy of newly of newly-developed collagen-based arterial closure device, Angioseal after transfemoral percuatenous coronary intervention. Methods : This study was designed as a prospective single center non-randomized comparative study. A total 200 patients undergoing transfemoral percutaneous coronary intervention were enrolled between April 2002 and May 2003. They were divided into two groups; Angioseal group (group A, n=100) and manual compression group (group B, n=100). The baseline clinical and angiographic characteristics were reviewed. The time to sit up, the time to ambulation, the duration of hospital stay, major and minor vascular puncture site complications were monitored. The patients were followed-up for 1 week after procedure by telephone. Results : The baseline clinical characteriwtics, clinical diagnosis, cardiocascular risk factors, typesof procedure, doses and numbers of anticoagulants were similar between two groups. The blood pressure and activated clotting time were also similar. The time to sit up (group A: 4.3±0.3 hours, group B: 13.7±0.8 hours, p=0.004) and the time to ambulation (group A: 6.8±0.5 hours, group B: 18.8±2.1 hours, p=0.013) were shorter in group A. No major vascular complications were noted. The incidence of hematoma and bleeding were not significantly different between two groups. The incidence of ecchymosis, however, was significantly lower in group A (group A:12%, group B: 3%, p=0.001) The duration of gospital stay was similar between groups. During 7 days of follow-up period incidence of hematoma was significantly lower in group A (p=0.004). Although the incidence of ecchymosis was not significantly different between two groups. Conclusion : The angioseal may be associated with earlier ambulation and less patients' morbidity with low incidence of local complication rate compared to manual compression after transfemoral percutaneous coronary intervention.

How Involvement Relates to the Formation of Attitudinal Loyalty, with Emphasis on Internet Leisure Activities

( Seok Pyo Hong ) 한국스포츠정책과학원(구 한국스포츠개발원) 2002 International Journal of Applied Sports Sciences Vol.14 No.1

Involvement was found to be the most important personal variable among others, such as motivation, locus of control, value choice, and degree of innovativeness in the formation of participant loyalty. Therefore, it is the most important variable that induces customer`s repeated patronage. As such, the degree of involvement in, and loyalty to, a product or service should be considered in developing the best marketing strategy that focuses on maintaining and increasing customer`s involvement and loyalty. Despite many studies regarding the relationship between involvement and attitudinal loyalty, casual relationship remains questionable due to the co-relational nature of those studies. The purpose of this study was to examine the relationship between the degree of involvement and attitudinal loyalty using the path analytic model, and also to determine relationships among indicators between the two constructs. A sample of 312 university students was used to examine the relationship between two constructs, and in filling out a questionnaire, respondents were asked whether they participate in any internet activities during their free time. They were also asked to report on their favorite internet leisure activity based on Jackson`s(1999) study of the internet in relation to leisure pursuits(i.e. games, chatting, music, information search, and e-mail). Structural Equation Modeling(SEM) and linear regression analysis were used for data analysis, and the following results were obtained through the data analysis. First, risk-related indicators for involvement and resistance to attitudinal loyalty were not significant indicators. Second, there was a strong relationship between involvement and attitudinal loyalty regarding internet leisure participation. Third, each indicator of involvement differently influenced each indicator of attitudinal loyalty.

A survey of the Brassica rapa genome by BAC-end sequence analysis and comparison with Arabidopsis thaliana /

Hong, Chang Pyo,Plaha, Prikshit,Koo, Dal Hoe,Yang, Tae Jin,Choi, Su Ryun,Lee, Young Ki,Uhm, Tae Sik,Bang, Jae Wook,Edwards, David,Bancroft, Ian,Park, Beom Seok,Lee, Jung Ho,Lim, Yong Pyo Korean Society for Molecular and Cellular Biology 2006 Molecules and cells Vol.22 No.3

The Relationship Between Zonulin and Asthma: A Mouse Model Study

Hong Joon-Pyo,Kang Sung Hun,Chu Jinah,Byeon Sun-Ju,Lee Hyunjoo,Choi Jeong-Hee,Lee Kyung Chul,Hong Seok Jin 대한비과학회 2023 Journal of rhinology Vol.30 No.3

Background and Objectives: Zonulin is a human protein that regulates intercellular tight junctions and increases the permeability of the intestinal epithelium. In light of the increasing focus on zonulin’s role in numerous chronic inflammatory diseases, this study aimed to investigate whether differences exist in serum zonulin levels and bronchial epithelium zonulin expression in vivo between asthma and normal groups, using a mouse model.Methods: Sixteen mice were utilized in this study, divided evenly between the normal and asthma groups. Serum zonulin levels, the expression of zonulin antibody in the bronchial epithelium, and serum cytokine levels were evaluated in both groups. Enzyme-linked immunosorbent assay and RNA in situ hybridization were utilized for the analysis.Results: The asthma group exhibited significantly higher levels of serum zonulin. High zonulin antibody expression was also observed in the bronchial epithelium of the asthma group. Given that our mouse model demonstrated a significant difference in interleukin (IL)-4 and IL-6 between the normal and asthma groups, zonulin may be associated not only with type 2 responses but also with various subtypes of asthma. Further studies are required to investigate this relationship in greater detail.Conclusion: Zonulin may play a role in the complex pathophysiology of asthma and could serve as a biomarker in various asthma-related situations.

Genomic distribution of simple sequence repeats in Brassica rapa

Hong, Chang Pyo,Piao, Zhong Yun,Kang, Tae Wook,Batley, Jacqueline,Yang, Tae Jin,Hur, Yoon Kang,Bhak, Jong,Park, Beom Seok,Edwards, David,Lim, Yong Pyo Korean Society for Molecular and Cellular Biology 2007 Molecules and cells Vol.23 No.3