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Hyun Na Koo,Sung Min Bae,Tae Young Shin,Jae Bang Choi,Bit Na Rae Yun,Jae Young Choi,Kwang Sik Lee,Jong Yul Roh,Yeon Ho Je,Byung Rae Jin,Soo Dong Woo 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05
The porcine reproductive and respiratory syndrome virus (PRRSV) has three major structural proteins which designated as GP4, GP5, and M. They have been considered very important to arouse the humoral and cellular immune responses against PRRSV infection and proposed to be the excellent candidate proteins in the design of PRRS bioengineering vaccine. However, the PRRSV structural proteins are produced in low levels in the infected cells because it forms insoluble protein and possesses several transmembrane regions. To overcome this problem, we fused the GP4, GP5, and M with SUMO (Small ubiquitin-related modifier), and expressed the fused gene in Bm5 cells and silkworm larvae. Expression of the proteins were analyzed by 12% SDS-PAGE and western blotting using 6xHis tag and porcine anti-PRRSV antibodies. In results, SUMO fused proteins were expressed at a high level in Bm5 cells. The levels of protein using the silkworm larvae is higher than that using Bm5 cells. The fused protein was purified by Ni-NTA affinity chromatography. This study demonstrated that SUMO, when fused with PRRSV structural proteins, was able to promote its soluble expression. This may be a better method to produce PRRSV structural proteins for vaccine development.
Small Cell Carcinoma of Ampulla of Vater: Report of Two Cases
Na Rae Kim,Yeon Ho Park,Yang Suh Koo,Seung-Yeon Ha,Dong-Hae Chung 대한췌담도학회 2010 대한췌담도학회지 Vol.15 No.1
We report a 62-year-old woman and a 66-year-old woman with abdominal pain and acute onset of jaundice, respectively. Preoperative cholangiopancreatography, cholangiography, and ERCP showed masses in the ampulla of Vater. Biopsy showed small cell carcinoma, and the tumor cells were immunoreactive for neuron-specific enolase, chromogranin A, and synaptophysin. Pancreatoduodenectomy or chemotherapy with radiation was performed in each patient. Histopathologic investigation disclosed direct invasion deep to the lamina propria, and infiltration of the pancreatic parenchyma, duodenum, and bile duct in the pancreatoduodenectomized patient. One patient died due to multiple liver metastases of the carcinoma of the ampulla of Vater 7 months after initiation of chemotherapy and radiotheraphy, and the other died 4 months after pancreatoduodenectomy. As in previously reported cases of small cell carcinoma of the ampulla of Vater, the present two cases showed extremely aggressive clinical course with early metastases resulting in fatal outcome.
Neurologic complications in patients with carotid artery stenting
Na Young Kim,Jong Wook Choi,황금,조성민,Youn Moo Koo,Jong Yeon Kim 대한뇌혈관외과학회 2019 Journal of Cerebrovascular and Endovascular Neuros Vol.21 No.2
Objective : Carotid artery stenting is helpful in patients with carotid artery stenosis and is a common method of treatment. However, data on the neurological consequences that might arise from, especially Asian patients after CAS is not enough. The purpose of this study was to investigate the outcome and prognostic factors affecting CAS patients. Methods : From January 2013 to June 2018 it was enrolled 97 patients who underwent CAS with severe carotid artery stenosis in a single institution. We retrospectively reviewed neurologic complications such as restenosis, ipsilateral or contralateral stroke, and hyperperfusion during the 6-month follow-up period. Results : There were no complication occured during the procedure in all 97 patients. Neurologic complications occurred in 30 patients (30.9%) after the procedure, and ipsilateral stroke 6 (6.2%), contralateral stroke 9 (9.4%), restenosis 2 (2.1%) and hyperperfusion 13 respectively. One of them had died (1.0%), of which the rest were discharged after symptoms improve. On univariate analysis, DM and pre-op NIHSS score was associated with the risk of CAS complication, exclusively. On the binary logistic regression for risk factors, DM (OR 0.144, 95% CI [0.029-0.718]), history of radiotheraphy (OR 36.103, 95% CI [1.009-1291.789]) and preoperative NIHSS (OR 1.266, 95% CI [1.059-1.513]) showed independent risk factors associated with post procedural neurological complications, statistically. Conclusion : Carotid artery stenting is a relatively safe and reliable long-term outcome for patients with carotid artery stenosis. However, careful observation should be taken after procedure immediately for any possible complications.
( Yeon Wook Kim ),( Na Kwon Kwak ),( Moon Woo Seong ),( Eui Chong Kim ),( Chul Gyu You ),( Young Whan Kim ),( Sung Koo Han ),( Jae Joon Yim ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: The Xpert MTB/RIF assay is endorsed by the WHO for detecting pulmonary and extrapulmonary tuberculosis (EPTB). The aim of this study was to determine the accuracy of the Xpert MTB/RIF assay in diagnosing EPTB in South Korea, which has an intermediate TB burden. Methods: We retrospectively reviewed the medical records of 1,426 patients in whom the Xpert MTB/RIF assay using extrapulmonary specimens was requested between January 1, 2011 and October 31, 2013 in a tertiary referral hospital in South Korea. The sensitivity, specifi city, positive predictive value (PPV) and negative predictive value (NPV) for diagnosis of EPTB and detection of rifampicin resistance were calculated. Results: Using culture as gold standard, sensitivity, specifi city, PPV, and NPV of the assay were 67.7%, 98.1%, 60%, and 98.6%, respectively. Higher sensitivity was shown among specimens including needle aspirates and biopsied tissues (85.7%), pus (75%), joint fi uids (100%), and urine (100%). The sensitivity, specifi city, PPV, and NPV for the detection of rifampicin resistance among specimens with positive results for M. TB were 80%, 100%, 100%, and 97.7%, respectively. Conclusions: The Xpert MTB/RIF assay showed acceptable sensitivity and excellent specifi city for diagnosis of EPTB and detection of rifampicin resistance in a country with an intermediate TB burden.