DJ-1/park7 modulates vasorelaxation and blood pressure via epigenetic modification of endothelial nitric oxide synthase

Won, Kyung Jong,Jung, Seung Hyo,Jung, Soo Hyun,Lee, Kang Pa,Lee, Hwan Myung,Lee, Dong-Youb,Park, Eun-Seok,Kim, Junghwan,Kim, Bokyung Oxford University Press 2014 Cardiovascular research Vol.101 No.3

<P><B>Aims</B></P><P>DJ-1/park7, a multifunctional protein, may play essential roles in the vascular system. However, the function of DJ-1/park7 in vascular contractility has remained unclear. The present study was designed to investigate whether the DJ-1/park7 is involved in the regulation of vascular contractility and systolic blood pressure (SBP).</P><P><B>Methods and results</B></P><P>Norepinephrine (NE) elevated contraction in endothelium-intact vessels in a dose-dependent manner, to a greater extent in DJ-1/park7 knockout (DJ-1/park7<SUP>−/−</SUP>) mice than in wild-type (DJ-1/park7<SUP>+/+</SUP>) mice. Acetylcholine inhibited NE-evoked contraction in endothelium-intact vessels, and this was markedly impaired in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP>. Nitric oxide (NO) production (82.1 ± 2.8% of control) and endothelial NO synthase (eNOS) expression (61.7 ± 8.9%) were lower, but H<SUB>2</SUB>O<SUB>2</SUB> production (126.4 ± 8.6%) was higher, in endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice than in those from DJ-1/park7<SUP>+/+</SUP> controls; these effects were reversed by DJ-1/park7-overexpressing endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice. Histone deacetylase (HDAC)-1 recruitment and H3 histone acetylation at the eNOS promoter were elevated and diminished, respectively, in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls. Moreover, SBP was significantly elevated in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls, but this elevation was inhibited in mice treated with valproic acid, an inhibitor of Class I HDACs including HDAC-1.</P><P><B>Conclusion</B></P><P>These results demonstrate that DJ-1/park7 protein may be implicated in the regulation of vascular contractility and blood pressure, probably by the impairment of NO production through H<SUB>2</SUB>O<SUB>2</SUB>-mediated epigenetic inhibition of eNOS expression.</P>

Finding hidden space of Coastal Protect area in Korea National Park

Jung Won Park,Sung Geon Jang,Jun Seong Kim 강원대학교 산림과학연구소 2017 강원대학교 산림과학연구소 학술대회 Vol.2017 No.11

There are 4 marine national parks in Korea, Hanryohaesang national park, Taeanhaean national park, Dadohaehaesang national park and Byeonsanbando national park. The total area of these park is 3,332.85㎢ that is 50.07% of the total area of all national parks in Korea, 21 site including mountain etc,. the areas exclusive land(mountain) is 2,753.709㎢ that is 40.9% of the total areas of all national parks in Korea. KNPS has been monitoring about the coastal wetland including mudflat and costal sanddune on 4 marine national park, and identified the total 105 site, 19 site of hanryohaesang national park, 35 site of Taeanhaean national park, 49 site of Dadohaehaesang national park and 2 site of byonsanbando national park and the area of 17.2㎢. This is 0.69% of the total area of mudflat in Korea and 7.8% of mudflat designated protected area in Korea. and also total length of shoreline is 1,729km that is 12% of the total length of shoreline in Korea. However, There is many unexplored coastal zone compared to the long shoreline. so, KNPS will start to research in-depth using technical equipment, for example, drone with camera for measurement, mutispectral scanner, liDAR(light detection and ranging) kinds of 3D scanner for ground, VRS/RTK GPS etc, for studying on the effective coastal zone information research system through precision surveying in 2017. And then, KNPS will carry out to expanding, restoring, monitoring and providing visitor service on special protection area of coastal zone. Precision survey results Increased from 17 square kilometers to 93 square kilometers in protect area

Insulin resistance mediates high-fat diet-induced pulmonary fibrosis and airway hyperresponsiveness through the TGF-β1 pathway

Park, Yoon Hee,Oh, Eun Yi,Han, Heejae,Yang, Misuk,Park, Hye Jung,Park, Kyung Hee,Lee, Jae-Hyun,Park, Jung-Won Nature Publishing Group UK 2019 Experimental and molecular medicine Vol.51 No.5

<▼1><P>Prior studies have reported the presence of lung fibrosis and enhanced airway hyperresponsiveness (AHR) in mice with high-fat-diet (HFD)-induced obesity. This study evaluated the role of TGF-β1 in HFD-induced AHR and lung fibrosis in a murine model. We generated HFD-induced obesity mice and performed glucose and insulin tolerance tests. HFD mice with or without ovalbumin sensitization and challenge were also treated with an anti-TGF-β1 neutralizing antibody. AHR to methacholine, inflammatory cells in the bronchoalveolar lavage fluid (BALF), and histological features were evaluated. Insulin was intranasally administered to normal diet (ND) mice, and in vitro insulin stimulation of BEAS-2b cells was performed. HFD-induced obesity mice had increased insulin resistance, enhanced AHR, peribronchial and perivascular fibrosis, and increased numbers of macrophages in the BALF. However, they did not have meaningful eosinophilic or neutrophilic inflammation in the lungs compared with ND mice. The HFD enhanced TGF-β1 expression in the bronchial epithelium, but we found no differences in the expression of interleukin (IL)−4 or IL-5 in lung homogenates. Administration of the anti-TGF-β1 antibody attenuated HFD-induced AHR and lung fibrosis. It also attenuated goblet cell hyperplasia, but did not affect the AHR and inflammatory cell infiltration induced by OVA challenge. The intranasal administration of insulin enhanced TGF-β1 expression in the bronchial epithelium and lung fibrosis. Stimulating BEAS-2b cells with insulin also increased TGF-β1 production by 24 h. We concluded that HFD-induced obesity-associated insulin resistance enhances TGF-β1 expression in the bronchial epithelium, which may play an important role in the development of lung fibrosis and AHR in obesity.</P></▼1><▼2><P><B>Obesity: A trigger for asthma onset</B></P><P>Insulin resistance may be an important causative factor underlying the increased risk of asthma and other respiratory issues in obese individuals. Obesity doubles the likelihood of developing asthma, with symptoms that are more difficult to control than in non-obese patients. The connection between these conditions is poorly understood, but researchers led by Jung-Won Park, Yonsei University College of Medicine, Seoul, South Korea, have identified a potential mechanism. They demonstrated that a signaling molecule called TGF-β1 contributes to airway sensitivity and tissue scarring in a mouse model of diet-induced obesity. Subsequent experiments showed that treatment with insulin also gives rise to increased TGF-β1 production in the mouse lung. Since insulin resistance is a common feature of obesity, resulting in abnormally high levels of circulating insulin, this could also account for the increased risk of respiratory problems.</P></▼2>

월악산국립공원의 부착규조 분포와 유기오탁지수 (DAIpo)에 의한 수질평가

박정원(Jung-Won Park) 국립공원연구원 2010 국립공원연구지 Vol.1 No.2

본 연구는 2006년에 월악산국립공원 내에 부착규조류 분포와 유기오탁지수에 의한 수질 평가이다. 동정된 부착규조류는 138종류였고, 우점종은 Achnanthes sp. Achnanthes alteragracillima, Achnanthes convergens, Achnanthes minutissima, Cocconeis placentula var. lineata, Cymbella affinis, Cymbella silesiaca, Gomphonema minutum, Reimeria sinuata 등이었다. DAIpo 값은 62.3에서 99.9의 범위로, 매우 좋음에서 좋음으로 평가되었다. To investigated the epilithic diatoms community and an assesment of water quality by DAIpo(diatom assemblage index of organic water pollution), the samplings were collected from fourteen sampling stations on April, May, August, September and October 2006 in Woraksan National Park. The total 138 taxa were identified from this study and dominant species were Achnanthes sp. Achnanthes alteragracillima, Achnanthes convergens, Achnanthes minutissima, Cocconeis placentula var. lineata, Cymbella affinis, Cymbella silesiaca, Gomphonema minutum, Reimeria sinuata. The variations of DAIpo values was ranged from 62.3 to 99.9 and the water quality was estimated between very good and good.

경주국립공원 문화재지정구역의 귀화식물 현황 및 관리방안

윤정원 ( Jung Won Yoon ),신현탁 ( Hyun Tak Shin ),이명훈 ( Myung Hoon Yi ),김기송 ( Gi Song Kim ),성정원 ( Jung Won Sung ),박기환 ( Ki Hwan Park ),이창현 ( Chang Hyun Lee ),김용식 ( Yong Shik Kim ) 한국환경생태학회 2012 한국환경생태학회지 Vol.26 No.4

경주국립공원 문화재지정구역에 분포하는 귀화식물은 총 14과 40속 45종 1변종 등 총 46분류군을 확인하였다. 귀화식물을 유형별로 구별해보면, 생활형은 1년생 초본이 17분류군(37%)으로 가장 많았으며, 다년생 초본이 16분류군 (34.8%), 2년생 초본이 11분류군(23.9%), 목본이 2분류군(4.3%)로 나타났다. 원산지 별로는 유럽이 17분류군(37%)으로 가장 많았으며, 그 다음으로 북아메리카에서 이입된 종이 16분류군(34.8%)으로 나타났다. 또한 귀화도는 3등급이 18분류군(40.9%)으로 가장 높다. 귀화식물 321분류군에 대한 본 연구대상지의 도시화 지수는 14.3%이며, 지구별 도시화 지수는 남산지구가 8.1%로 가장 높다. 경주국립공원은 사적형 공원으로 문화재가 매우 중요하나, 문화재 주변의식재 및 관리 또한 중요하다. 따라서 귀화식물의 경우 장기적인 모니터링과 생태적인 특성을 파악하여 체계적인 관리방안이 요구된다. The naturalized plant species in Gyeongju National Park was summarized as 46taxa including 14 families 40 genus 45 species 1 varieties. Among them, annual herbaceous species was 17taxa (37%) which is grouped as the biggest proportion and 16taxa (34.8%) for perennial herbaceous species and 11taxa (23.9%) for biannual herbaceous species and 2taxa (4.3%) for tree species. European origin was the biggest proportion as 17taxa (37%), and 16% (34.8%) from North America. The degree of naturalization (DoN) was the biggest for Degree III, V as 18taxa (39.1%). Regarding 321 naturalized plant species, the Urbanization Index in the studied area was 14.3%. The Namsam District showed 8.1% which is the highest DoN in the studied districts within the Gyeongju National Park. As the cultural properties is important in the Gyeongju National Park as a representatives for historical sites in Korea, planting and its management in the cultural properties is important tasks. The naturalized plant species in the studied area should be reasonably management.

경주국립공원의 관속식물상

윤정원 ( Jung Won Yoon ),김용식 ( Yong Shik Kim ),신현탁 ( Hyun Tak Shin ),김기송 ( Gi Song Kim ),성정원 ( Jung Won Sung ),이창현 ( Chang Hyeon Lee ),박기환 ( Ki Hwan Park ),이명훈 ( Myung Hoon Yi ) 한국환경생태학회 2013 한국환경생태학회지 Vol.27 No.2

본 연구는 경주국립공원(남산, 토함산, 단석산)의 관속식물상을 밝히고 주요 식물들의 분포를 조사하였다. 2011년 7월부터 2012년 5월까지 총 7회 조사하였다. 조사결과 관속식물은 96과 284속 385속 4아종 41변종 6품종으로 총 436분류군을 확인하였다. 그 중 산림청 지정 희귀식물은 7분류군, 특산식물은 13분류군이다. 식물구계학적 특정식물은 41분류군으로 Ⅴ등급 1분류군, Ⅳ등급 3분류군, Ⅲ등급 9분류군, Ⅱ등급 4분류군,Ⅰ등급 24분류군이다. 한편 귀화식물은 29분류군으로 확인하였으며, 귀화율은 6.65%, 도시화지수는 9.03%이다. 조사된 436분류군의 유용도는 목초용 자원식물이 182분류군(41.7%), 식용 176분류군(40.4%), 약용 147분류군(33.7%), 관상용 52분류군(11.9%), 목재용 18분류군(4.1%), 염료용 16분류군(3.7%), 섬유용 8분류군(1.8%), 공업용 자원식물이 1분류군(0.2%) 그리고 용도가 밝혀지지 않은 식물이 79분류군(18.1%)으로 나타났다. This study was carried out to investigate the vascular flora of Mt. Nam, Mt. Toham, and Mt. Danseok in Gyeongju National Park . The vascular plants were surveyed for 7 times from July 2011 to May 2012. The results were summarized as 436 taxa, 96families, 284 genera, 385 species, 4 subspecies, 41 varieties, and 6 forms. The 13 taxa were categorized as Korean endemic plants species and the other 7 taxa as rare plants species, which categorized by the Korea Forest Service. Based on the Floristic Degrees categorized by the Specific Distribution of Plants Species, one taxa grouped as the Floristic Degree Ⅴ, 3 for Floristic Degree Ⅳ, and 9 for Floristic Degree Ⅲ, 4 for Floristic Degree Ⅱ, and 24 taxa for Floristic Degree I. The naturalized plants were recorded as 29 taxa, and their Naturalization Ratio and Urbanization Index were recorded as 6.65%, and 9.03%, respectively. 436 taxa listed consists of 182 taxa(41.7%) of pasturing plant, 176 taxa(40.4%) of edible plants, 147 taxa(33.7%) of medicinal plants, 52 taxa(11.9%) of ornamental plants, 18 taxa(4.1%) of timber plants, 16 taxa(3.7%) of stain plants, 8 taxa(1.8%) of fiber plants, 1 taxa(0.2%) of industrial plants and 79 taxa(18.1%) of unknown plants.

민간공원특례사업에 있어 갈등요인 분석과 해소방안 연구

이정하(Lee Jung Ha),박태원(Park Tae Won) 도시정책학회 2018 도시정책학회 학술대회 Vol.2018 No.1

Since the Constitutional Court ruled in July 1999 that the private urban planning facilities were subject to the violation of private property rights, the government has adopted a sunset system for urban parks. While the private business owner s size of urban parks was lowered from 80 % to 70 % in 2014, the cause of the controversy over the project was changed from 100,000㎡ or more in total urban parks to 50,000㎡ or more. Therefore, this study analyzes the causes of failure in the public park formation projects based on the cases in which conflicts are emerging in analyzing similar systems abroad and promoting projects, and analyzes the causes of failure through related experts and surveys. In addition, the causes of conflicts are different, local sentiments and development conditions are different, and the limit of urban park management and response plans for future urban urban planning based on projects focused on the business feasibility and retrofittibility are limited, and urban park plans are less than 50,000㎡.

生長調節物質 處理가 고추品種의 生育 및 基他特性에 미치는 影響

朴惠遠,李政明 경희대학교 부설 식량자원개발연구소 1989 硏究論文集 Vol.10 No.-

A series of experiments were undertaken to find out effects of several plant growth regulators on the growth and other factors in several cultivars of pepper (Capsicum spp.). Major results can be summarized as follows. 1. Paclobutrazol greatly reduced the shoot elongation and, in general, the higher the concentration, the severe the growth inhibition. 2. Soil treatment of uniconazole was most effective in retarding the shoot elongation. However, the plants treated with uniconazole also exhibited dark green foliage and late flowering. 3. Plant treated with CCC exhibited early flowering, good fruit set, and early fruit pigmentation. 4. SADH sprayed at 5,000 to 10,000 ppm was effective in reducing the plant size suitable for attractive pot plants. 5. MH treatment completely inhibited the elongation of main shoot, but not the growth of axillary shoots. 6. The growth retarding effect of paclobutrazol and uniconazole was proven to persist much longer than other chemicals for a single spray treatment.

초기 성공적인 심폐소생술후 혈청 Interleukin-6 농도의 발현양상

박규남,박승현,황두영,김형국,이환,최경호,이원재,정욱성,김학주,김세경 大韓應急醫學會 1998 대한응급의학회지 Vol.9 No.4

Objective : To investgate patterns of serial serum interleukin-6(IL-6) concentrations after initial successful cardiopulmonary resuscitation, and to access differences in serum IL-6 concentrations in patients with Cerebral Performance Category(CPC, 1-2) and CPC(3-5) at the 5th day after cardiacpulmonary resuscitation(CPR). Methods : We studied prospectively 12 patients with spontaneous circulation after cardiopulmonary resuscitation. Venous blood samples were taken 2, 4, 12, 24, 48 and 72 hours after restoration of spontaneous circulation to measure serum IL-6 levels by the enzyme-linked immunosorbent assay. Results : Serum IL-6 concentrations in the severe neurolgically disabled group(CPC, 3-5) were significantly increased at 2, 24 and 48 hours after restoration of spontaneous circulation compared with the less neurolgically disabled group. Also, serum IL-6 concentrations in the severe neurolgic disabled group showed dual peak at 4 and 24 hours. Conclusion : Our study demonstrates significant increase of serum IL-6 in patients with CPC(3-5) after successful cardiopulmonary resuscitation and the significant correlation between early increase of serum IL-6 and poor neurologic outcome. It suggests that early increase of serum IL-6 can be used as a prognostic marker after successful cardiopulmonary resuscitation.

응급실로 내원한 장중첩증의증 환아의 진단방법에 대한 임상적 고찰

원형섭,박정배,표창해,김형수,박철완,이근 대한응급의학회 1995 대한응급의학회지 Vol.6 No.2

The purpose of this study is to evaluate the appropriate diagnostic methods for the patients with suspected intussusception. Intrssusception is a state that a portion of alimentary tract is telescoped into a segment just caudad to it, and has a characteristic symptoms of cyclic irritability, vomiting, bloody stools and palpable mass, It is the most common cause of acute or acquired intestinal obstruction in infants and children, and needs early diagnosis and immediate treatment. Authors reviewed retrospectively medical records of 411 infants & children with suspected intussusception from January 1, 1992 to December 31, 1994 and report the results with literature reviews. The results were as followings; 1) 263 cases were definite intussusception(DI) out of 411 suspected intussusception(SI). 2) The male to female ratio was 2.3 : 1 in SI and 2.8 : 1 in DI. 3) The distribution of age revealed that 85% of SI and 84% of DI were under 24 months old, and the most common age was 8 months in SI and 10 months in DI. 4) There were upper respiratory infection in 84 cases(2)%), gastroenteritis in 32 cases(8%) and bronchiolitis in 8 cases(2%) as preceding diseases. 5) The simple X-ray showed gaseous bowel distension in 212 cases(52%). 6) The most common symptoms and signs were cyclic irritability and abdominal pain(94%), bloody stools(72%), palpable abdominal mass(47%), in order. 7) Of 411 patients, 252 cases(61%) were diagnosed and treated by air enema(228 cases) and/or exploration(24 cases). Among 296 cases(72%) who showed bloody stools in digital rectal examination and glycerin enema, 245 cases(83%) were confirmed as intussusception through air enema and/or operative intervention, and that corresponds to 93% of DI. 8) Intestinal perforation as complication occurred in 3 of 393 cases who underwent air and barium enema, but all of them recovered without any sequelae after operation. Conclusively, it is thought that air enema preceded by digital rectal examination and glycerin enema is safe and useful method in diagnosis and treatment of infants and children with SI.