Comparative Study between PLGA and Dermal Tissue for Tracheal Regeneration

( Jin Hoon Kim ),( Ho Joong Kim ),( Jhin Gook Kim ),( Won Ho Kong ),( Soo Won Seo ) 한국조직공학과 재생의학회 2010 조직공학과 재생의학 Vol.7 No.3

Various kinds of biomaterials that have biocompatibility and biodegradability have been used for the tissue engineered organ reconstruction. Their characteristics have relationship with functional and morphological reconstruction. However, it is not clear which types of biomaterials provide the best outcomes after transplantation. For the reason, in this study, to compare synthetic biomaterial and natural biomaterial, we developed two kinds of patches to compare affects of biomaterials for cell transplantation and organ reconstruction. The one was coated with PLGA sheet and the other was coated with autologous dermal sheet. Cultured PKH26-labeled cells were seeded on the patches and then the patches were transplanted onto tracheas. After three months, we found that each biomaterial had a different tracheal reconstruction pattern: the PLGA patch was suited for airway patency(tracheal passage with stable inside diameter for air exchange) but not for epithelial layer reconstruction whereas the dermal patch was suited for epithelial layer reconstruction but not for airway patency. Consequently, while selecting biomaterials for transplantation and reconstruction, not only their biological effects on the body but also their effects on organ morphology and function should be considered.

Optimized Serological Isolation of Lung-Cancer-associated Antigens from a Yeast Surface-expressed cDNA Library

( Min Soo Kim ),( Hye Young Choi ),( Yong Soo Choi ),( Jhin Gook Kim ),( Yong Sung Kim ) 한국미생물생명공학회 2007 Journal of microbiology and biotechnology Vol.17 No.6

Increased expression of ICAM-3 is associated with radiation resistance in cervical cancer

배덕수 ( Duk Soo Bae ),( Young Min Chung ),( Byoung Gie Kim ),( Chang Soo Park ),( Seung Jae Huh ),( Jhin Gook Kim ),( Jong Kuk Park ),( Sun Mi Cho ),( Byung Soo Kim ),( Jun Suk Kim ),( Young Do Yoo ) 대한산부인과학회 2006 대한산부인과학회 학술대회 Vol.92 No.-

N2 병기 비소세포 폐암의 수술 전 동시화학방사선요법

이규찬(Kyu Chan Lee),안용찬(Yong Chan Ahn),박근칠(Keunchil Park),김관민(Kwhan Mien Kim),김진국(Jhin Gook Kim),심영목(Young Mog Shim),임도훈(Do Hoon Lim),김문경(Moon Kyung Kim),신경환(Kyung Hwan Shin),김대용(Dae Yong Kim),허승재(Seung 대한방사선종양학회 1999 Radiation Oncology Journal Vol.17 No.2

목 적:N2 병기 비소세포폐암에서 수술 전 동시화학방사선요법과 수술을 적용하여 급성 부작용, 수술절제가능성 및 수술 후 병기 강하율을 보고하고자 한다. 대상 및 방법:1997년 5월부터 1998년 6월까지 삼성서울병원에서 N2 병기 비소세포폐암으로 진단 받은 15명의 환자들을 대상으로 하였다. 환자들 연령의 중앙값은 61세(45∼67세), 남녀 성비는 12:3 이었으며 세포 조직형은 편평상피암종이 11명, 선암종이 4명이었다. 치료 전 임상적 T병기는 T1, T2, 및 T3가 각각 2명, 12명, 및 1명씩이었으며 모든 환자들이 N2 병기로서 이중 10명은 종격동내시경을 통한 조직생검을 통하여 종격동 림프절 전이를 확인하였고 나머지 5명은 흉부 CT 영상 상 종격동 림프절 전이가 강력히 의심된 경우였다. 수술 전 방사선치료는 흉부 CT 영상을 기준으로 하여 원발 병소, 동측 폐문부 및 종격동 림프절에 대하여 10MV X-선을 이용하여 45Gy를 5주간에 걸쳐서 조사하도록 계획하였다(일회 선량 1.8Gy, 일일 1회, 주 5회). 수술 전 복합화학요법은 cis-Platin(100mg/m2) 을 제 1일에 급속 정주하고 Etoposide(50mg/m2/day)를 제 1일부터 제 14일까지 경구로 2회에 분복하도록 하였으며, 첫 번째 화학요법은 방사선치료의 시작일에, 두 번째 화학요법은 4주 후에 시행하도록 계획하였다. 수술은 방사선-복합화학요법의 완료 3주째에 흉부 CT 영상을 얻어 병변의 진행이나 원격전이의 소견이 없음을 확인한 후 시행하였다. 결 과:방사선치료는 15명 모두에서 계획된 목표 선량 45Gy를 조사하였으며, 복합 화학요법은 11명의 환자에서는 계획대로 2회를, 나머지 4명에서는 1회만 시행하였다. 본 연구의 치료 방법과 관련하여 1명의 환자가 수술 15일만에 급성 호흡부전으로 사망하였으며 입원치료를 요하는 정도의 급성 부작용의 발현은 방사선폐렴과 호중구 감소로 인한 발열이 각각 1명, 2명이었다. 급성 식도염은 RTOG grade 1이 9명, grade 2가 3명으로 대체로 경미한 편이었다. 총 26회의 복합 화학요법과 관련된 급성 부작용으로 grade 3 이상의 백혈구 감소증, 혈소판 감소증, 및 빈혈이 각각 26.9%, 7.7%, 3.8%에서 나타났다. 수술을 시행한 환자는 13명으로 이 중 12명에서 근치적 절제술이 가능하여 수술절제율은 92.3%(12/13) 이었다. 한 명에서는 수술 시 늑막 전이가 확인되어 절제 수술을 시행하지 못하였다. 다른 2명은 수술을 거부하였다. 수술 후 병리학적 T병기는 T0, T1, 및 T2가 각각 3명, 6명, 및 3명이었으며 N병기는 N0, N1, 및 N2가 각각 8명, 1명, 및 3명이었다. 병리학적 완전 관해는 모두 3명의 환자에서(27.3%) 확인되었으며, 수술 전 임상적 병기와 비교하여 볼 때 병기 강하, 불변, 상승이 각각 8명(61.5%), 4명(30.8%), 1명(7.7%) 이었다. 결 론:N2 병기 비소세포폐암에 대한 동시화학방사선요법은 대체로 만족할 만한 결과를 얻을 수 있었으나 보다 많은 환자들을 대상으로 하는 장기간의 추적 관찰을 요한다. Purpose:This is to evaluate the acute complication, resection rate, and tumor down-staging after pre-operative concurrent chemoradiotherapy for stage IIIA (N2) non-small cell lung cancer. Materials and Methods:Fifteen patients with non-small cell lung cancer were enrolled in this study from May 1997 to June 1998 in Samsung Medical Center. The median age of the patients was 61 (range, 45∼67) years and male to female ratio was 12:3. Pathologic types were squamous cell carcinoma (11) and adenocarcinoma (4). Pre-operative clinical tumor stages were cT1 in 2 patients, cT2 in 12, and cT3 in 1 and all were N2. Ten patients were proved to be N2 with mediastinoscopic biopsy and five had clinically evident mediastinal lymph node metastases on the chest CT scans. Pre-operative radiation therapy field included the primary tumor, the ipsilateral hilum, and the mediastinum. Total radiation dose was 45 Gy over 5 weeks with daily dose of 1.8 Gy. Pre-operative concurrent chemotherapy consisted of two cycles of intravenous cis-Platin (100 mg/m2) on day 1 and oral Etoposide (50 mg/m2/day) on days 1 through 14 with 4 weeks' interval. Surgery was followed after the pre-operative re-evaluation including chest CT scan in 3 weeks of the completion of the concurrent chemoradiotherapy if there was no evidence of disease progression. Results:Full dose radiation therapy was administered to all the 15 patients. Planned two cycles of chemotherapy was completed in 11 patients and one cycle was given to four. One treatment related death of acute respiratory distress syndrome occurred in 15 days of surgery. Hospital admission was required in three patients including one with radiation pneumonitis and two with neutropenic fever. Hematologic complications and other acute complications including esophagitis were tolerable. Resection rate was 92.3% (12/13) in 13 patients excluding two patients who refused surgery. Pleural seeding was found in one patient after thoracotomy and tumor resection was not feasible. Post-operative tumor stagings were pT0 in 3 patients, pT1 in 6, and pT2 in 3. Lymph node status findings were pN0 in 8 patients, pN1 in 1, and pN2 in 3. Pathologic tumor down-staging was 61.5% (8/13) including complete response in three patients (23.7%). Tumor stage was unchanged in four patients (30.8%) and progression was in one (7.7%). Conclusion:Pre-operative concurrent chemoradiotherapy for Stage IIIA (N2) non-small cell lung cancer emonstrated satisfactory results with no increased severe acute complications. This treatment scheme deserves more patient accrual with long-term follow-up.

천명음과 폐기능검사로 찾아 낸 상기도종양

김진국,이경수,김호중,안용찬 대한내과학회 2002 대한내과학회지 Vol.62 No.1

Molecular profiles of EGFR, K-ras, c-met, and FGFR in pulmonary pleomorphic carcinoma, a rare lung malignancy

Lee, Soohyeon,Kim, Youngwook,Sun, Jong-Mu,Choi, Yoon La,Kim, Jhin Gook,Shim, Young-Mog,Park, Yeon Hee,Ahn, Jin Seok,Park, Keunchil,Han, Jung Ho,Ahn, Myung-Ju Springer-Verlag 2011 Journal of Cancer Research and Clinical Oncology Vol.137 No.8

<P><B>Background</B></P><P>Pulmonary pleomorphic carcinoma (PPC) is a rare type of lung cancer characterized by the poor response to conventional chemotherapy and subsequent disappointing outcomes. Therefore, it is paramount to delineate the molecular characteristics of this disease entity.</P><P><B>Methods</B></P><P>In this study, we retrospectively examined the surgical specimens of 61 patients who underwent lung surgery. Mutational or gene amplification statuses of epidermal growth factor receptor (EGFR), <I>k</I>-<I>ras</I>, <I>c</I>-<I>kit</I>, <I>c</I>-<I>met</I>, and fibroblast growth factor receptor (FGFR) were examined using genomic DNA sequencing, real-time PCR and/or fluorescence in situ hybridization (FISH).</P><P><B>Results</B></P><P>The median age was 61 years, and 50 patients were men and 11 were women. In the histologic review of epithelial component, adenocarcinoma were in 44 cases (72%), squamous cell carcinoma in 15 (25%) and large cell carcinoma in 2 patients (3%). Overall, 30 cases (49%) had any molecular alterations. Nine patients (15%) possessed EGFR deletion in exon 19 (<I>n</I> = 8) or L858R mutations in exon 21 (<I>n</I> = 1), while 3 other cases having atypical EGFR mutations. Six patients (9.8%) had <I>k</I>-<I>ras</I> mutations in exon 12, and 3 had <I>c</I>-<I>kit</I> mutations. High gene copy number of <I>c</I>-<I>met</I> was found in 11 patients (18.0%) and that of FGFR was in 6 patients (9.8%). No significant relationships were identified among the occurrence and type of mutations and patient survival or any other clinicopathological variables.</P><P><B>Conclusions</B></P><P>Given the diverse repertoire of mutational profiles observed in PPC samples, clinical trials based on accurate cancer-genotyping should be considered as a legitimate treatment scheme for this rare disease entity in the future.</P>

대동맥 박리증의 수술요법 -27례의 수술환자를 대상으로 한 5 년간의 성적-

김진국,안혁,Kim, Jhin-Gook,Ahn, Hyuk 대한흉부심장혈관외과학회 1988 Journal of Chest Surgery (J Chest Surg) Vol.21 No.3

Current therapy of aortic dissections remains unstandardized because of the relative rarity of these catastrophic events and conflicting reported results of various therapeutic strategies. Hence, we reviewed our current results and planned to purify our method of interpretation of results and so, to standardize therapeutic managements. This study comprised unselected, consecutive 27 patients with aortic dissections who were operated at Seoul National University Hospital from Jan 1983 to March 1988. The results from analysis of their preoperative, operative and postoperative finding were as follows: 1] 7 patients had acute type A, 14 had chronic type A, 4 had acute type B, and 2 had chronic type B. 2] The causes of dissections were unclear, but 8 patients had Marfan`s syndromes, 2 had previous operative histories on cardiovascular systems and 2 had congenital heart diseases. 3] Multiple preoperative variables were found to correlate significantly with operative mortality and complications. The prevalences of such preoperative major complicating factors were significantly more frequent in acute than chronic [P < 0.05] and type A than type B [P < 0.01]. 4] Operations were performed according to the type of the dissections and whether it was acute or chronic. Usually dacron tube graft replacements were performed[25/26]. Intraluminal sutureless graft replacement was performed in 11 patients. Of the 14 patients with combined aortic regurgitation, concomitant aortic valve resuspension in 4, seperative aortic valve replacement in 1, and aortic valve replacement with coronary reimplantation were performed in 9 patients. 2 patients had concomitant arch vessel managements. 5] Over-all operative mortality rate was 33% and 54% for acute type A, 25% for acute type B, 29% for chronic type A, 0% for chronic type B respectively. The main causes of operative mortality were cardiovascular complications [mainly CPB-weaning failure] in acute cases and hemorrhagic complications in chronic cases.

누드 랫드의 호모자웅간 번식 성공사례의 보고

김희진(Heui-Jin Kim),박종성(Jong-Seong Park),김진국(Jhin-Gook Kim),한진수(Jin-Soo Han) 한국실험동물학회 1998 Laboratory Animal Research Vol.14 No.1

전혈류 정지술을 이용한 하공정맥 폐색증의 교정수술

김진국,나명훈,안혁,Kim, Jhin-gook,Na, Myung-Hoon,An, Hyuk 대한흉부심장혈관외과학회 1987 Journal of Chest Surgery (J Chest Surg) Vol.20 No.4

MOVC is an uncommon disease which can be corrected by surgical method if early detected. A case of a 34-year-old male with MOVC is reported. Operation was done on cardiopulmonary bypass with circulatory arrest under moderate hypothermia. The incision was done both on the RA extending to level of suprahepatic IVC and on the IVC just proximal to the right renal vein. And then, thrombectomy and membranectomy under the direct visualization was done. Total circulatory arrest was used intermittently in order to get good visual field and for preventing blood loss. The Postop. course was good except one episode of hepatic encephalopathy which was persisted for 12 hours and then controlled by conservative measures.

봉합사와 대망을 이용한 도관형 장기재건

김진훈 ( Jin Hoon Kim ),김진국 ( Jhin Gook Kim ),서수원 ( Soo Won Suh ) 한국조직공학과 재생의학회 2006 조직공학과 재생의학 Vol.3 No.4

To reconstruct the tube type organs like trachea, vessel, stomach, esophagus, bladder and intestine, two considerations have to solve that the first is to endure the suture strength of surgical operation and the movement of organ, and the second is to guarantee the bioactivity of cell and tissue through angiogenesis. Because the previous materials have weak strength or low biocompatibility and difficult to be supplied blood vessels in terms of the wide range replacement, differently with skin, new material or operation method are required. In this study, we are trying to solve the above problems and the possibility of making artificial organ by cell culture and implantation using the polyglycolide woven mesh scaffold(WMS) and the omentum.