폐렴구균의 용혈능 변화와 pneumolysin 유전자의 변이 양상

오원섭,장현하,정숙인,김연숙,이혁,김신우,김성민,백경란,이남용,송재훈 대한화학요법학회 2002 대한화학요법학회지 Vol.20 No.3

목적 : 폐렴구균은 인체에서 폐렴, 수막염 및 패혈증 등을 일으키는 중요한 병원균으로 현재 전 세계적으로 항균제 내성이 큰 문제가 되고 있다. 하지만 폐렴구균의 항균제 내성에 따라 병독성이 어떻게 변화하는지에 대해서는 구체적으로 알려져 있지 않다. 이에 기능적 및 구조적 분석을 통하여 폐렴구균의 주요 병독성 인자인 pneumolysin의 병인론적 역할을 규명하고자 하였다. 방법 : 임상 검체에서 분리된 페니실링 감수성 균주 20주와 내성 균주 20주를 대상으로 하였다. Pneumolysin의 세포 독성의 변화를 관찰하기 위하여 각각의 균주들의 용혈능을 측정하였고, pneumolysin 유전자를 중합효소 연쇄반응으로 증폭한 후 증폭 산물의 유전자 구조를 분석하였다. 결과 : 페니실린 감수성 균주와 내성 균주간의 용혈능은 유의한 차이를 보이지 않았으나, 일부 용혈능이 매우 낮은 균주에서 Lys152→Thr, Thr-172→Ile, Lys-224→Arg와 같은 변이가 관찰되었다. 결론: pneumolysin의 세포 독성의 변화는 항균제 내성 정도와는 무관하였으나, 일부 용혈능이 저하된 균주에서 발견된 pneumolysin 유전자의 변이가 pneumolysin의 세포 독성에 영향을 주는 것으로 보인다. Background : Pnumolysin (Ply) is one of the most important virulence factors of Streptococcus pneumoniae. To investigate the functional and structural variation of Ply, hemolytic activity (HA) and gene sequences of pneumolvsin were determined. Methods : A total of 40 invasive pneumococcal isolated (20 penicillin-susceptible & 20-resistant strains) were analyzed. HA of each isolate was measured in crude extracts (1×10^8 CFU/mL) hourly from the time of inoculation to 9h. Crude extracts (50㎕) and DTT buffer (100㎕) were serially diluted in 96-well plate and mixed with 1% sheep blood (50㎕). HA was represented as the reciprocal of the greatest dilution, which resulted in the complete lysis. Two sets of oligonucleotide primers were used to amplify a 1,474-bp fragment containing the coding region and a 578-bp fragment of upstream region of ply gene, respectively. Amplified products were directly sequenced. Results : HA showed a wide variation from 0 to 87,480 regardiess of penicillin MICs, serotypes or specimen sources. Two bacteremic strains showed completely no hemolytic activity. No significant differences in HA between penicillin-susceptible (Mean± SE : 15,468± 4,693) and penicillin-resistant strains (21,384± 4,087) were found (p > 0.05).Sequencing of the coding region showed unique alterations in amino acids in strains with markedly reduced hemolytic activity (≤ 40): Thr-172-Ile and Lys-224-Arg. However, upstream region of ply gene was highly conserved. Conclusions : Ply activity was not related with antibiotic resistance. Absence of HA in some strains was associated with unique alterations in amino acid sequences in ply gene. Relationnship between genomic alteration and functional changes in pneumolysin should be further investigated.

2010 파주, 안산지역 갈대, 억새 등 야초류의 이용실태와 사료가치 평가

서성(Sung Seo),김원호(Won Ho Kim),정민웅(Min Woong Jung),박형수(Hyung Soo Park),심재진(Jae Jin Shim),박진길(Jin Gil Park),성하균(Ha Guyn Sung),김종덕(Jong Duk Kim),이종경(Joung Kyong Lee) 한국초지조사료학회 2011 한국초지조사료학회지 Vol.31 No.2

본 연구는 부존 조사료자원의 이용을 활성화하여 수입사료를 줄이고 가축 사료비를 절감하고자 2010년도에 경기 파주 (민통선 지역)와 경기 안산 (시화지구)에서 갈대와 억새 등 야초자생지를 탐색하고, 수확?이용실태 조사와 함께 사료가치를 분석하였다. 파주 민통선 내억새 (Miscanthus sinensis)와 갈대 (Phragmites communis) 혼합 자생지는 90~100 ㏊ 규모로, 1차 곤포작업으로 사일리지 520톤 (㏊당 6톤), 2차로 380톤 (㏊당 4톤)을 조제하여, 연 사일리지 900톤 (㏊당 10톤)을 생산하였다. 곤포의 유통가격은 롤당 52~55천 원으로 ㎏당 130~137.5원이었다. 곤포는 자가소비 50~70%, 판매유통 30~50%로, 수익성은 인건비 정도로 추정되었다. 초장 70 ㎝의 재생 억새는 조단백질 9.6%, 상대사료가치 82.4 (건초 4등급), 건물 소화율 67.7%, 초장 70 ㎝의 재생 갈대는 각각 13.8%, 84.3 (4등급), 67.9%로 사료가치는 높았으며, 수확이 늦어짐에 따라 사료가치는 크게 낮아졌다. 안산 시화지구 (3공구)의 갈대 등 야초류자생지는 100 ㏊ 정도로 2009년도에는 50 ㏊에서 사일리지 550톤 (㏊당 11톤)을 생산하여 롤당 50천 원에 유통하였으며, 2010년도는 잦은 강우와 장마로 부분 이용에 그쳤다. 고초기의 갈대는 건물률 89.8%, 조단백질 2.2%, NDF 80.6%, ADF 55.4%, 상대사료가치 52.9 (5등급), 건물 소화율 36.4%로 볏짚에 비해서도 사료가치는 크게 낮았다. 초장 80 ㎝의 재생 갈대는 조단백질 12.9%, 상대사료가치 99.8 (3등급), 건물 소화율 66.6%로 사료가치는 양호하였으며, 개화기의 초장 150 ㎝ 갈대는 조단백질 4.5%, 상대사료가치 59.9 (5등급), 건물 소화율 42.2%로 볏짚의 사료가치와 비슷하였다. 이상의 결과를 종합하여볼 때, 갈대, 억새 등 야초류는 현 상태에서 최대한 수거?이용하되, 사료가치를 유지하고 가축 기호성을 고려한 시기에 수확하는 것이 중요하며, 최소한 볏짚과 비슷하거나 그 이상의 사료가치를 기대하기 위해서는 7월 중순 이전 (늦어도 7월까지)에 수확?이용하는 것이 권장된다. 본 연구에서 조사된 대부분의 야초류는 상대사료가치가 75 이하인 건초 5등급으로 사료가치는 매우 낮았으며, 키가 작은 영양생장기에 수확한 경우에만 4등급으로 나타났다. 부존 조사료자원의 이용은 양질의 사료작물 생산과 병행하여 추진하는 것이 바람직하였다. This study was carried out in 2010 to investigate the utilization and nutritive value of native grasses, such as Phragmites communis and Miscanthus sinensis which might reduce the cost of feeding domestic cattle. The regions within the Civilian Control Line in Paju and Sihwa region in Ansan were surveyed. In Paju, the yearly silage production was 900 MT harvested from 90 ha (10 MT/㏊). About 30~50% of these were sold out at the marketing price of 52,000~55,000 Won per roll (130~137.5 Won/㎏). Regrown Miscanthus sinensis of 70 ㎝ in length contained 9.6% of crude protein (CP), 82.4 of relative feed value (RFV), and 67.7% of in vitro dry matter digestibility (IVDMD). Regrown Phragmites communis of 70 ㎝ in length contained 13.8% of CP, 84.3 of RFV, and 67.9% of IVDMD. As harvesting was delayed, the quality was decreased dramatically. In Ansan region, Phragmites communis plantation covers 100 ha. The silage production was 550 MT from the area of 50 ha (11 MT/㏊) in 2009. And the marketing price was 50,000 Won per roll. But in 2010 only a small amount of grasses could have been baled due to frequent and much rainfall from spring to summer season. However, the good forage quality was observed from regrown Phragmites communis of 80 ㎝ in length, 12.9% CP, 99.8 RFV and 66.6% IVDMD, while that of late matured grasses was very low, 2.2% CP, 52.9 RFV and 36.4% IVDMD, greatly lower than forage quality of rice straw. The quality of grasses at bloom stage of 150 ㎝ in length was similar to that of rice straw, showing 4.5% CP, 59.9 RFV and 42.2% IVDMD. In conclusion, the overall quality of most native grasses in this survey were very poor. Therefore, we recommend that Phragmites communis and Miscanthus sinensis should be harvested during June or July to obtain better forage quality which is richer in forage values than rice straw. Production of high quality forage crops was also desirable for self sufficiency of forage.

ACCase 저해 제초제 cyhalofop-butyl에 대한 경남지방 수집종 피의 저항성

원종찬,원옥재,하준,임일빈,강광식,변종영,박기웅,이증주,Won, Jong Chan,Won, Ok Jae,Ha, Jun,Im, Il-Bin,Kang, Kwang Sik,Pyon, Jong Yeong,Park, Kee Woong,Lee, Jeung Joo 한국잡초학회한국잔디학회 2018 Weed & Turfgrass Science Vol.7 No.2

Repeated use of ACCase inhibiting herbicides for a long time has resulted in increases of resistant Echinochloa oryzicola populations in paddy fields in middle west area of Korea. This study aims to investigate current status of herbicide resistant E. oryzicola in Gyeongsangnam-do, in which there is less information about herbicide resistance. For resistance frequency and dose-response study, seeds from 100 individual plants of E. oryzicola in Gyeongsangnam-do were collected and tested with cyhalofop-butyl. Seven percent of plants from Gyeongsangnam-do was resistant at a recommended rate of cyhalofop-butyl. $GR_{50}$ values (herbicide rates required to reduce plant growth 50%) for one representative resistant populations and five susceptible populations were $738g\;a.i.\;ha^{-1}$ and 66-234 (average 147)$g\;a.i.\;ha^{-1}$, respectively, indicating average 5 times difference in resistance. Although lower rate of frequency of herbicide resistance in Gyeongsangnam-do than in Jeollabuk-do, increases of herbicide resistance are expected in this area because of increases of direct seeded rice fields and increases of dependence on a specific herbicide. Therefore, it is necessary to monitor herbicide resistance regularly and conduct integrated herbicide resistance management in this area. 경남지역 제초제 저항성 피의 발생현황과 저항성 정도를 알아보기 위하여 벼 재배 논에서 100개체의 피 종자를 수집하여 ACCase 저해제인 cyhalofop-butyl에 대한 약량반응 실험을 수행한 결과, 수집한 피의 7%가 저항성으로 조사되었다. 선발된 감수성 5개체의 $GR_{50}$값은 평균 $147g\;a.i.\;ha^{-1}$로 나타났고, 함양 지역의 저항성 개체의 $GR_{50}$값은 $738g\;a.i.\;ha^{-1}$ 로 R/S (ratio of resistance to sensitivity)값은 5.01배로 나타났다. 현재 경남지역에서의 저항성 피의 발생 수준은 낮은 것으로 나타났지만, 저항성 피의 확산을 지연시키거나 막기 위해서는 주기적인 저항성 피의 모니터링과 체계적인 저항성 잡초 관리방안을 마련하여 수행할 필요가 있다.

치골결합의 외상성 분리에 대한 치료

하상호,유재원,이상홍,신동민,표영배,안병관 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.16 No.2

The separation of symphysis pubis as a result of injury is uncommon, but it is increasing in number in parallel with today's speedy development of car industry. Most cases of the separation of symphysis pubis are amenable to closed treatments, but unstble separation where the reduction is difficult to maintain may require open techniques. Authors analyzed 27 cases of traumatic separation of symphysis pubis with 16 months follow-up on an average, who had been treated at the Orthopaedic Department, Chosun University Hospital. The results obtained were as follows; 1. The most common cause was traffic accident (81.5%). 2. Among of 27 patients, instability on the posterior stabilizer of pelvic bone was noticed in 18 patients. 3. Fifteen patients were treated with closed methods(bed rest, hammock, traction)and twelves were treated by the open reduction and internal fixation. 4. Internal fixations were carried out with plate and screw(5 cases), screw and wiring(4 cases), and wiring (3 cases). 5. The separation of symphysis pubis with minimal disruption of posterior stabilizer were treated by closed treatment or internal fixation of the separation of symphysis pubis diastasis alone, and its result was good or excellent. 6. We think that the symphysis pubis diastasis with severe disruption of posterior stabilizer should be fixed anteriorly and posteriorly both.

영양소제거 공정의 기질 정량관계에 대한 온도와 기질부하의 영향

하준수,최의소,박재홍,길경익,김성원 대한상하수도학회 2003 상하수도학회지 Vol.17 No.6

This research has been performed to evaluate the stoichiometric relationship between removed COD and nitrogen or phosphorus. A modified A²/O process, which was equipped with an external nitrification tank and a switching reactor, was operated with a capacity of 50㎥/d. From the results, it has been concluded that the stoichiometric values were varied by temperature condition or input COD loads. In case of denitirfication, the requirement g COD for removed nitrate nitrogen ranged 0.13 to 10.5g, and the compensation coefficient of temperature was evaluated as 1.028. For phosphorus removal, the required COD was about 7.5gCOD_(consumed)/gP_(released) and 0.54gPHACOD_(storage)/gP_(uptake). During rainy season, as it appeared a lower organic substrate loads, the PHA(Poly Hydroxy Alkanoate) contents of biomass was reduced. From the relationship between influent BOD, and the sum of removed TN and TP, the ratio for rainy and winter season was about 4.8 and 5.8g BOD/g (TN+TP) each others. Therefor, the substrat ratios for nitrogen and phosphorus removal appeared to be affected by temperature and influent organic substrate loads.

경골 분절 골절의 골수강내 금속정을 이용한 수술적 치료

하상호,유재원,이상홍,신동민,표영배,허민 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.2

The Fracture of the tibia is the most common on fracture of the long bone and has high complication rates due to it's anatomical property. Segmental fractures are characterized by two or more fractures on different levels of the shaft with an intermediate segment. In addition to general characteristics of tibial fracture, segmental fractures are commonly combined with extensive soft tissue injury comminution and displacement with poor blood supply in it's intermediate segment. Recently, closed intramedullary nailing under the image intensifier has been widely used and its good results have been reported. The purpose of this study is to compart the bony union, functional result and complication between Ender nail and interlocking nail which we treated with intramedullary nailing in 20 segmental tibial fractures of 19 patients from 1990 to 1991. Minimum follow up period was 12 months. The results were as follows : 1. The most common type of fractures was Melis type Ⅰ. 2. All fractures were united and average union time was 24.9 weeks. 3. The major complications were delayed union (6 cases) joint stiffness (6 cases), superficial infection (3 cases), and angular deformity (2 cases). The complication rate in Ender nail was higher than Interlocking nail. 4. According to the criteria of functional results by Klemm and Borner. we obtained a 87.5% of excellent and good resulted in Ender nailing group and 91.7% of excellent and good results in Interlocking nail group.

중간세공탄소를 담체로 사용한 직접메탄을 연료전지용 Pt/C 촉매제조

하승백,김현수,최재식,정원섭,이호인 한국공업화학회 2003 응용화학 Vol.7 No.2

For the direct methanol fuel cell, the expensive cost of Pt is one of the most important factors. In this study, various kinds of support were synthesized and chosen for highly dispersed catalysts which need less amount of Pt and show higher activity. A mesoporous carbon was selected as a support which has 5 times larger specific surface area than Vulcan XC-72, a commercial support. Such a large surface area gave an advantage in the dispersion of Pt, but a disadvantage in electric conductivity which could be solved by increasing the carbonizaton temperature in the preparation of mesoporous carbon. These prepared mesoporous carbons were used as supports of Pt. Among the catalysts, the catalyst using mesoporous carbon carbonized at 1000 ℃ showed better activity than the catalyst using Vulcan XC-72 as a support showing the best activity. From the XRD analysis, the activity was proportional to the dispersion of Pt.

난소의 호산성 투명세포암종 - 1예의 보고 -

하창원,고재수,명나혜,조경자,박상윤,김미경,장자준 대한병리학회 1992 Journal of Pathology and Translational Medicine Vol.26 No.5

무방향 네트워크 그래프에서의 효율적인 경로계산알고리즘

하경재,김원경 경남대학교 정보통신연구소 1999 연구논문집 Vol.1 No.-

컴퓨터, 통신, 전력계통 등의 신뢰도 분석과 같은 문제에 네트워크의 확률그래프모델이 지난 수십년동안 널리 사용되고 있으며, 이 경우 모델 그래프의 출발지(source)와 목적지 사이의 경로(paths) 집합이나 cutset 정보를 이용하여 문제를 해결하는 방법이 많이 제시되고 있다. 이러한 경우 아무리 잘 작성된 신뢰도분석 알고리즘이라 할지라도 네트워크규모가 커질수록 대부분의 수행시간은 신뢰도계산 등에 소요되는 시간보다는 경로셋이나 컷셋 등을 구하는(enumerating) 데 대부분 소요된다. 따라서, 네트워크 그래프모델을 사용하면서 주어진 문제를 해결하는 새로운 알고리즘을 개발하고 실제 구현하였다면 전체 수행결과의 분석을 위해 이들 셋집합을 구하기 위한 알고리즘과 그 수행시간 및 메모리요구량 등에 대한 분석을 반드시 언급해야만 한다. 본 논문에서는 네트워크시스템의 신뢰도분석 등의 문제에 사용되는 네트워크 그래프모델에서, 임의의 s-t 두 노드쌍 사이의 모든 최소경로를 찾는 효율적인 알고리즘을 제안한다. 이 알고리즘은 네트워크그래프의 접속행렬 대한 소행렬식 전개와 대수적 부울연산에 근거하며, 계산속도와 메모리요구량을 줄일 수 있도록 개선되었다. Probabilistic graph models have been widely used for the past few decades as a model for reliability analysis of computer, communication, and electric power systems. Especially, the problem of reliability calculation for a s-coherent system such as computer networks are based on paths or cutsets between source and destination nodes. Most of time spent in a well-written program will be on enumerating path sets or cutsets, rather than on calculating the reliability. When development and implementation of a new algorithm on the reliability analysis is reported, the researcher should report the corresponding path enumeration algorithm and its running time, storage required. In this paper, we proposed an efficient algorithm for enumerating all minimal paths of a source-terminal pair in the network graph. The proposed algorithm is based on simple boolean operations through the minor expansion of a connection matrix and can improve the performance in terms of the computing time and storage.

교정용 각형선재에 부여된 torque가 브라켓에 발생시키는 모멘트에 관한 유한요소법적 분석

하도원,김영석,성재현 대한치과교정학회 2001 대한치과교정학회지 Vol.31 No.3

교정용 각형선재에 부여된 torque가 브라켓에 발생시키는 모멘트에 관한 유한요소법적 분석 본 연구의 목적은 교정치료의 마무리 단계에서 바람직하지 못한 순설측 경사를 가진 한 개 치아의 이동을 원할 때 브라켓 슬롯의 크기와 각형선재의 종류,크기에 따라 부여하여야 할 적절한 임상적 torque(SWA의 각형선재에서 나머지 편평한 부분과 이동을 위해 변형시킨 부분의 단면이 이루는 각도만큼의 torque)의 양을 구하고자 하는 것이다 임상적 torque는 play와 active torque(브라켓에 모멘트를 전달할 수 있는 torque)의 합으로 구성되는데, play는 수학적 공식을 이용하여 계산하였고 arrive torque는 컴퓨터를 이용한 삼차원 유한요소 법으로 구하였다. 유한요소모델은 일렬로 배열된 세 개의 브라켓과 여기에 삽입된 stainless steel, TMA, NiTi 이렇게3가지 종류의 교정용 각형선재로 구성된다. 양쪽의 브라켓을 일정한 각도로 비틀어서 가운데 브라켓에 발생하는 모멘트를 계산하였다. 선재의 크기는 많이 사용되는 각형선재인 .016" X .022", .017" X .022", .017" X .025", .018" X .025", .019" X .025" .020" X .025", .021" X .025"의7개로 디자인하였다. .018" 브라켓에는 .016" X .022", .017" X.022", .017" X .025" 선재를 삽입하여 실험하였고 .022" 브라켓에는 .016" X .022" 선재을 제외한 나머지 전재를 삽입하여 실험하였다. 실험으로 다음과 같은 결과를 얻었다. 1.삽입된 브라켓 슬롯의 크기에 상관없이 같은 크기와 재질의 교정용 각형선재에 같은 active torque를 가하면 동일한 모멘트가 발생하였다. 2.선재의 크기가 증가될 수록 동일한 active torque에 의해 발생되는 모멘트의 양은 증가하였다. 실험에 사용한 가장 굵은 선재인 .021" × .025" 선재는 동일한 재질의 가장 가는 .016" X .022" 전재에 비해 약 1.75배 더 큰 torsional stiffness를 가졌다. 3.선재의 재질에 따라서는 stainless steel, TMA, NiTi순으로 torsional stiffness가 감소하였는데 stainless steel에 비해 TMA는 0.35배, NiTi는 0.16배 였다. 4.브라켓간 거리의 증가와 발생되는 torsional stiffness는 반비례하였다 브라켓간 거리의 증가에 의해 감소되는 torsional stiffness의 비율은 선재의 재질과 상관이 있었고 크기에 따라서는 큰 차이가 없었다. 5.교정치료의 마무리 단계에서 이상적인 순설측 경사이동을 일으키는 임상적 torque의 공식과 값을 구하였다. (주요 단어 : 임상적 토오크, 액티브 토오크, 모멘트, 유한요소법) The purpose of this study was to investigate the ideal clinical torque(In the SWA rectangular wire, the torque by the angle between the plane part and twisted part to move the tooth) of the orthodontic rectangular wire which produce the proper labiolingual movement of the single tooth during finishing stage of the orthodontic treatment. The clinical torque is the sum of the play and the active torque which generates the moment at the bracket. The play is calculated by the formula and the active torque is calculated by the computer aided three-dimensional finite element method. The finite element model was consist of the three brackets which formed a row and 3 kinds of orthodontic rectangular wire(stainless steel, TMA, NiTi) which inserted in brackets. Both sides of the model were twisted and the moment generated in the center bracket was calculated. The sizes of seven wires which were used commonly were .016" X .022", .017" X .022", .017" X .025", .018" X .025", .019" X .025", .020" X .025", .021" X .025". In 018" bracket, 016" X .022", ,017" X .022", .017" X .025" wires were inserted and in 022" bracket, all the sizes of wires except .016" X .022" were inserted and tested. The following conclusion could be drawn from this study. 1.The moment generated on the same size of the wires by the same active torque were equal regardless of the bracket slot size. 2.The moment were increased with the size of the wires. The moment generated on the .021" X .025" wire was about 1.75 times as large as that on the .016" X .022" wire regardless of the material. 3.The moments were increased in the order of the NiTi, TMA, stainless steel. The moment of the TMA wire was 0.35 times as small as that of the stainless steel wire and the moment of the NiTi was 0.16 times as small as that of the stainless steel wire. 4.The moment was decreased as the interbracket distance was increased. 5.To get a desired moment with the specific size and material of the wire on the specific bracket slot, the formula and the results were displayed. Key words : Clinical torque, Active torque, Moment, Finite element method