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Serratia marcescens에서 cAMP receptor protein(CRP)유전자의 클로닝 및 해석
유주순,문종환,정수열,김혜선,최용락 東亞大學校附設遺傳工學硏究所 1999 遺傳工學硏究 Vol.- No.6
전사조절인자로서 잘 알려져 있는 cAMP receptor protein(CRP)은 cAMP와 DNA에 결합하는 특별한 활성을 가지고 있으며, cAMP-CRP complex를 형성하여 수많은 유전자의 발현조절에 관여한다. 이러한 측면에서 cAMP-CRP의 조절은 어떤 면에서 총체적 조절체계라고까지 한다. 본 연구는 Serratia 균주에서 crp유전자의 분자적 특성 및 cAMP에 의한 발현조절을 받는 분자기구를 해석하고자 유전자를 클로닝하고 발현을 확인하였다. MacConkey배지에서 maltose를 탄소원으로 충분히 이용하지 못하는 대장균 TP2139(△crp,△lac)를 숙주로 이용하고, 염색체DNA를 library로 작성하여 얻은 형질전환체 약 일만개의 콜로니에서 red colony를 나타내는 5종류 의 양성 클론을 얻었다. 이들 클론을 Southern방법으로 확인한 결과 3kb의 단편을 가진 pCKB12클론이 crp유전자를 coding하고 있음을 확인하였다. glpD-lacZ 융합 plasmid인 pLDC6 의 BamHI부위에 pCKB12의 3kb 단편을 삽입시킨 재조합 plasmid pLDC 6-Scrp를 작성하여, 클로닝된 Serratia 의 crp 유전자가 대장균에서 유전자 전사조절에 미치는 영향을 확인한 결과 cAMP-CRP 복합체 형성에 의한 전사조절 기능이 확인되어졌다. One of the better-characterized transcription factor of E. coli is the cAMP receptor protein(CRP) and the CRP binds cAMP and DNA. The cAMP-CRP complex is involved in regulation of many genes at bacteria. The cAMP-CRP regulatory element represents, in some respects, a global regulatory network. The aim of this work was to study the structure and the mechanisms controlling the expression of CRP in Serratia marcescens. We have been get 5 different clones from Serratia which stimulated the cells to use maltose as a sole carbon source in E. coli TP2139. The crp gene clone, pCKB12, was confirmed by Southern hybridization with E. coli crp gene. The location of the crp gene was determined by constructing subclones carrying various portions of pCKB12. To investigate the potential role of CRP in E. cloi, lacZ fused plasmids were constructed and investigated the β-galactosidase activity of the fused plasmid. The Serratia marcescens cAMP receptor protein can substitute the E. coli CRP in transcriptional activation at the lacZ gene. These results suggest that Serratia marcescens cAMP receptor protein complex functions to regulate several promoters in E. coli.
Risk Based Inspection 기법을 이용한 화학공장의 안전성 향상에 관한 연구
노용해,유진환,서재민,임차순,고재욱 한국산업안전학회 2002 한국안전학회지 Vol.17 No.3
The RBI technique proposed by API is composed of three steps. The qualitative RBI method can be used for the purpose of screening the components with high risk. And, the quantitative RBI method employs complex risk evaluation model for predicting component risk in a quantitative manner. The inspection program can be optimized based on the results obtained by these RBI technique. The forementioned RBI technique has been applied to a common hydrodesulfurizer unit and the technique is critically evaluated for studying its benefits and limitations, which is the main issue of this thesis. It's conducted that the RBI method can provide a method for defining and measuring the component risk, and also provide a powerful tool for managing many of the important elements of a process plant.
( Hae Won Yoo ),( Young Seok Kim ),( Sang Gyune Kim ),( Jeongju Yoo ),( Jae Woo Park ),( Yong Seok Lim ),( Gab Jin Cheon ),( Jae Young Jang ),( Young Don Kim ),( Soung Won Jeong ),( Sae Hwan Lee ),( B 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1
Aims: The Baveno VI guidelines proposed that esophagogastroduodendoscopy(EGD) for screening esophageal varices(EV) can be avoided if liver stiffness(LS) measured by transient elastography(TE) is less than 20 kPa and platelet count is greater than 150,000 cells/μL. However while validation of TE is well proven, 2D-SWE has not been sufficiently validated for EV prediction. The aim of this study is to predict the presence of EV by non-invasive tools combined with 2D-SWE and to compare the diagnostic capabilities with TE. Methods: Between January 2015 and October 2017, 258 patients with compensated advanced chronic liver disease(cACLD) who underwent 2D-SWE and EGD consecutively were enrolled. The AUROC was calculated to evaluate the accuracy of the prediction for the presence of EV using 2D-SWE, model combining 2D-SWE and platelet count(PC), liver stiffness to spleen/platelet score (LSPS) score and platelet-spleen ratio (PSR) score. 177 patients who underwent simultaneous TE examination were on the subgroup analysis was done. Results: The mean age was 56.8±10.7 years and most common etiology was chronic hepatitis B (45.0%). 83.1% of patients were in Child class A. Prevalence of all-size varices was 41.1%. 2D-SWE alone has good ability to discriminate varices (AUROCs : 0.750, 95% CI, 0.690 to 0.809). Model combining 2D-SWE with platelet count and LSPS using 2D-SWE has better discriminative ability for varices and AUROCs were 0.793 (95% CI, 0.738 to 0.848) and 0.813 (95% CI, 0.760 to 0.865) respectively. For 177 patients who performed TE and 2D-SWE simultaneously, there was no difference in predictive abilities when other factors such as albumin, bilirubin, ALT, Platelet count, hemoglobin, spleen diameter were adjusted in multivariate analysis. Conclusions: 2D-SWE combined with platelet count and LSPS seemed to be useful to predict EV. In addition, 2D-SWE has similar diagnostic performance with TE for predicting presence of EV.
The Role of Spleen Volume in Predicting Portal Hyper-tension in Patients with Cirrhosis
( Hae Won Yoo ),( Sang Gyune Kim ),( Young Seok Kim ),( Jeongju Yoo ),( Jae Woo Park ),( Yong Seok Lim ),( Jae Myeong Lee ),( Gab Jin Cheon ),( Jae Young Jang ),( Young Don Kim ),( Soung Won Jeong ),( 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1
Aims: It is already well known that the size of the spleen increases as liver cirrhosis aggravates. However, there is few studies for establishing the correlation between portal hypertension and spleen volume (SV). In this study, we aimed to see the relationship between the splenic volume and hepatic venous wedge pressure (HVPG) and to find what determine the portal pressure. Methods: From May 2007 to February 2018, 337 cirrhotic patients who measured SV and HVPG simultaneously were included. SV was measured based on the ellipsoid volume calculation formula by measuring length, height, and width. Other clinical and biochemical factors which may contribute to increase portal pressure were also collected. Results: Mean HVPG was 14.2 ± 5.3 and Child-pugh class A, B, and C patients were 34%, 40%, and 16%, respectively. A significant positive correlation was noted between splenic volume and HVPG levels (r=0.127, P=0.019). Below 16mmHg of HVPG, the stronger correlation was noted between them (r=0.202, P=0.003) but this relation was not observed above 16mmHg of HVPG. We divided HVPG into various stages of portal hypertension(HVPG<5, 5≤HVPG<10, 10≤HVPG<16, HVPG≥16). The average spleen volume of each group tended to increase with increasing HVPG value. Mean splenic volume for each group was 320.74cm3, 464.78 cm3, 501.06 cm3, 525.94 cm3 respectively. But there was no significant difference in the mean spleen volume between the groups., HVPG was correlated with presence of ascites (P=0.038) as well as SV (P=0.043) after adjusted by MELD, Na, albumin, platelet count. Conclusions: The spleen volume tended to increase with increasing HVPG and this association was stronger when HVPG was below 16 mmHg. More studies with larger numbers are needed to predict the portal pressure non-invasively.
A recurrent inactivating mutation in RHOA GTPase in angioimmunoblastic T cell lymphoma
Yoo, Hae Yong,Sung, Min Kyung,Lee, Seung Ho,Kim, Sangok,Lee, Haeseung,Park, Seongjin,Kim, Sang Cheol,Lee, Byungwook,Rho, Kyoohyoung,Lee, Jong-Eun,Cho, Kwang-Hwi,Kim, Wankyu,Ju, Hyunjung,Kim, Jaesang,K Nature Publishing Group, a division of Macmillan P 2014 Nature genetics Vol.46 No.4
The molecular mechanisms underlying angioimmunoblastic T cell lymphoma (AITL), a common type of mature T cell lymphoma of poor prognosis, are largely unknown. Here we report a frequent somatic mutation in RHOA (encoding p.Gly17Val) using exome and transcriptome sequencing of samples from individuals with AITL. Further examination of the RHOA mutation encoding p.Gly17Val in 239 lymphoma samples showed that the mutation was specific to T cell lymphoma and was absent from B cell lymphoma. We demonstrate that the RHOA mutation encoding p.Gly17Val, which was found in 53.3% (24 of 45) of the AITL cases examined, is oncogenic in nature using multiple molecular assays. Molecular modeling and docking simulations provided a structural basis for the loss of GTPase activity in the RHOA Gly17Val mutant. Our experimental data and modeling results suggest that the RHOA mutation encoding p.Gly17Val is a driver mutation in AITL. On the basis of these data and through integrated pathway analysis, we build a comprehensive signaling network for AITL oncogenesis.
柳海鏞(Yoo Hae-Yong) 한국법학원 2005 저스티스 Vol.- No.83
다른 모든 자유의 뿌리가 되는 신체의 자유를 극도로 제한하는 인신구속은 형사소송절차의 정당한 목적 달성을 위하여 불가피한 경우에만 예외적으로 허용되어야 한다는 것이 우리나라의 헌법과 형사소송법의 기본정신이고, 대법원과 헌법재판소 판례도 이를 강조한다. 영장주의는 인신구속절차에 대한 법원의 견제를 통해 구속의 남용으로부터 개인의 인권을 보호하려는 안전장치인데 이러한 제도적 보장이 형사소송의 실무운영에 그대로 반영되는 것이 중요하다. 1997년부터 시행된 영장실질심사제도는 구속의 목적과 올바른 운영방식 등을 둘러싼 다양한 논쟁의 기폭제가 되었으며, 수사 초기 단계의 인권침해 여지 제거, 구속되는 피의자의 법관대면권 보장, 적법절차의 강화, 불필요한 구속의 억제 등 여러 가지 성과를 남겼으나, 「구속=처벌」 관행을 완전히 극복하지 못하였고, 함께 도입된 조기 석방장치인 기소 전 보석제도는 제 역할을 하지 못하는 등 한계도 드러났다. 이 글에서는 현행 인신구속제도 운영상 문제점을 인신구속에 관한 시각 차이로 인한 혼선, 석방제도의 제한적 활용, 형사절차 전반에 대한 체계적 접근 부족, 합리적ㆍ구체적 구속기준 및 석방기준의 부재, 구속의 본래 목적 외 활용, 미결구금에 의존하는 양형, 일반 국민의 이중적 법 감정 등 측면으로 나누어 분석하고, 이러한 운영상 결함이 초래하는 부작용으로 피의자ㆍ피고인의 방어권 침해, 무죄추정 원칙의 형해화, 구속 및 석방을 둘러싼 사법 불신, 공판중심주의 제약 등을 지적하였다. 이러한 여러 가지 문제점에 대한 해결방안으로 사법개혁위원회가 제안한 구속대체제도 도입, 석방제도 통합, 석방수단의 다양화 등이 입법에 반영되는 것을 염두에 두면서 사안의 중대성과 도망할 염려 사이의 연관성에 대한 합리적 해석을 통한 「구속=처벌」 연결고리의 부분적 해체, 기소 전 보석의 적극 활용, 구속적부심사ㆍ보석 전담부의 운영, 집행유예제도의 보완 등을 제시하고 있다.