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Soybean Trypsin Inhibitor 와 황산 콘드로이친 포합체의 약리 효과 평가
최윤림(Youn Lim Choi),남현규(Hyun Gu Nam),신영희(Young Hee Shin) 한국약제학회 2000 Journal of Pharmaceutical Investigation Vol.30 No.3
Kunitz-type soybean trypsin inhibitor (SBTI) and chondroitin sulfate (A, and C type) were conjugated using sodium periodate method. And the physicochemical, pharmacokinetic properties and immunogenecity of the conjugates (Chon-A-SBTI or Chon-C-SBTI) were characterized. We expected the conjugation using chondroitin sulfate to reduce the immunogenecity and to improve the pharmacological effect. As the results, the mean molecular weight of the conjugate highly increased. After I.V. injection of the radiolabeled conjugates or native SBTI into mice, it was found that native SBTI showed rapid elimination from plasma, whereas Chon-A-SBTI and Chon-C-SBTI were slowly eliminated. Organ distribution of the two agents at 30 min after I.V. injection was different : Chon-A-SBTI or Chon-C-SBTI accumulated to a large extent in the liver (13% in Chon-A-SBTI and 16% in Chon-C-SBTI), whereas native SBTI was taken up more rapidly by the kidney (107% dose/g of tissue) and excreated into the urine (26%). In addition we evaluated the therapeutic value of the conjugates by using the sublethal septic shock model caused by pseudomonal elastase and tested the immunogenecity by passive cutaneous anaphylaxis shock (PCA). The conjugates were more effective than native SBTI against pseudomonal elastase induced septic shock in guinea pig. In case of the conjugates, the pharmacological and therapeutic effect lasted over 3 hours long. In immunogenecity test, both of the conjugates showed the reduction of their immunogenecity, especially Chon-A-SBTI looked most effective.
Jung, Nam-Chul,Lee, Jun-Ho,Choi, Hyun-Ji,Hwang, Sung-Uk,Song, Jie-Young,Seo, Han Geuk,Choi, Jinjung,Jung, Sang Youn,Han, Sung Gu,Lim, Dae-Seog Informa UK (TaylorFrancis) 2016 Immunological investigations Vol.45 No.6
<P>Background: The response of hepatocellular carcinoma (HCC) to immunotherapy is often disappointing and new strategies are clearly needed. The aim of the present study was to investigate whether cytokine-induced killer (CIK) cells combined with a dendritic cell vaccination enhanced cytotoxicity against hepatocarcinoma tumor cells in an in vivo animal model.Methods: CIKs and DCs were prepared from C3H/HeJ mice by conventional methods, the dendritic cell (DC) pulsed with a MH134 cell lysate, DC or CIK alone were used as controls. Cell phenotypes were analyzed by flow cytometry, cytokine secretion levels were determined by enzyme-linked immunosorbent assay (ELISA), and cytotoxicity was assessed by means of an in vitro lactate dehydrogenase (LDH) release assay. A mouse hepatocarcinoma cell MH134-bearing mice model was established to test the in vivo anti-tumor efficacy of the system.Results: CIK cells combined with DC therapy resulted in significant inhibition of tumor growth compared with the control group, whereas the decrease in tumor growth in mice that had been treated with CIK or DC alone did not reach the level of statistical significance. The combination therapy led to a further increase in the population of cytotoxic T cells (CTLs) in vivo, compared to the CIK or DC alone therapy. In addition, the combination therapy significantly enhanced cytotoxic activity against MH134 cells.Conclusion: Taken together, these results show that a DC + CIK vaccination is more effective than DC or CIK alone therapy for the treatment of hepatocarcinoma cancer.</P>
GaN HPA MMIC 기반 Ka 대역 25 W SSPA 설계 및 제작
지홍구(Hong-gu Ji),노윤섭(Youn-sub Noh),최윤호(Youn-ho Choi),곽창수(Chang-soo Kwak),염인복(In-bok Youm),서인종(In-jong Seo),박형진(Hyung-jin Park),조인호(In-ho Jo),남병창(Byung-chang Nam),공동욱(Dong-uk Kong) 한국전자파학회 2015 한국전자파학회논문지 Vol.26 No.12
Ka 대역의 25 W급 SSPA를 제작하기 위하여 상용 0.15 μm GaN 공정을 이용 구동증폭기(Drive Amplifier : DRA) 및 고출력증폭기(High Power Amplifier : HPA) 초고주파 단일 집적회로(Monolithic Microwave Integrated Circuit : MMIC)를 설계 및 제작하여 특성 평가하였고, SSPA(Solid State Power Amplifier)의 주요 부속품인 MS-to-WR28 변환기 및 WR28 전력합성기를 설계 및 제작, 평가하여 Ka 대역 GaN 기반 SSPA를 제작하였다. 제작 결과, 주파수 29~31 GHz 대역에서 포화출력 44.2 dBm 이상, 전력부가효율 16.6 % 이상, 전력이득 39.2 dB의 특성을 나타내었다. We designed and manufactured Ka-band SSPA include drive amplifier and high power amplifier MMICs by 0.15 μm GaN commercial process. Also, we fabricated main components micro-strip line to WR28 waveguide transition and WR28 wave guide power combiner for Ka-band SSPA. This Ka-band SSPA shows saturated output power 44.2 dBm, power added efficiency 16.6 % and power gain 39.2 dB at 29~31 GHz frequency band.
SWAT ArcView GIS Extension Patch를 이용한 소유역 분할에 따른 수문 및 유사 거동에 미치는 영향 평가
허성구 ( Sung Gu Heo ),김남원 ( Nam Won Kim ),박윤식 ( Youn Shik Park ),김종건 ( Jong Gun Kim ),김성준 ( Seong Joon Kim ),안재훈 ( Jae Hun Ahn ),김기성 ( Ki Sung Kim ),임경재 ( Kyoung Jae Lim ) 한국물환경학회 2008 한국물환경학회지 Vol.24 No.2
Because of increased nonpoint source runoff potential at highland agricultural fields of Kangwon province, effective agricultural management practices are required to reduce the inflow of sediment and other nonpoint source pollutants into the water bodies. The watershed-scale model, Soil and Water Assessment Tool (SWAT), model has been used worldwide for developing effective watershed management. However, the SWAT model simulated sediment values are significantly affected by the number of subwatershed delineated. This result indicates that the SWAT estimated watershed characteristics from the watershed delineation process affects the soil erosion and sediment behaviors. However, most SWAT users do not spend time and efforts to analyze variations in sediment estimation due to watershed delineation with various threshold value although topography falsification affecting soil erosion process can be caused with watershed delineation processes. The SWAT model estimates the field slope length of Hydrologic Response Unit (HRU) based on average slope of subwatershed within the watershed. Thus the SWAT ArcView GIS Patch, developed by using the regression relationship between average watershed slope and field slope length, was utilized in this study to compare the simulated sediment from various watershed delineation scenarios. Four watershed delineation scenarios were made with various threshold values (700 ha, 300 ha, 100 ha, and 75 ha) and the SWAT estimated flow and sediment values were compared with and without applying the SWAT ArcView GIS Patch. With the SWAT ArcView GIS Patch applied, the simulated flow values are almost same irrespective of the number of subwatershed delineated while the simulated flow values changes to some extent without the SWAT ArcView GIS Patch applied. However when the SWAT ArcView GIS Patch applied, the simulated sediment values vary 9.7% to 29.8% with four watershed delineation scenarios, while the simulated sediment values vary 0.5% to 126.6% without SWAT ArcView GIS applied. As shown, the SWAT estimated flow and sediment values are not affected by the number of watershed delineation significant compared with the estimated flow and sediment value without applying the SWAT ArcView GIS Patch.
IM-412 inhibits the invasion of human breast carcinoma cells by blocking FGFR-mediated signaling.
Jung, Seung-Youn,Yi, Jae Youn,Kim, Mi-Hyoung,Song, Kyung-Hee,Kang, Seong-Mook,Ahn, Jiyeon,Hwang, Sang-Gu,Nam, Ky-Youb,Song, Jie-Young National Hellenic Research Foundation 2015 Oncology reports Vol.34 No.5
<P>Triple-negative breast cancer (TNBC) is an aggressive cancer with a poor prognosis due to its epithelial???to-mesenchymal transition (EMT) phenotype. Cancer patients often experience several detrimental effects of cancer treatment, such as chemoresistance, radioresistance and the maintenance of cancer stem cells due to EMT. Thus, EMT signaling is considered to be a valuable therapeutic target for cancer treatment, and its inhibition is being attempted as a new treatment option for TNBC patients. Previously, we showed that 3-(2-chlorobenzyl)-1,7-dimethyl-1H-imidazo[2,1-f]purine???2,4(3H,8H)-dione (IM-412) inhibits transforming growth factor-관 (TGF-관)-induced differentiation of human lung fibroblasts through both Smad-dependent and??-independent pathways. In the present study, we examined the inhibitory effect of IM-412 on EMT pathways and invasiveness in TNBC cells since the TGF-관 signaling pathway is a typical signaling pathway that functions in EMT. IM-412 not only potently suppressed the migration and invasion of MDA-MB-231??cells, but also lowered the expression of mesenchymal markers and EMT-activating transcription factors in these cells. IM-412 inhibited the activation of several signaling proteins, including Smad2/Smad3, p38MAPK, Akt and JNK, and it also attenuated the phosphorylation of FGFR1 and FGFR3. Collectively, our findings suggest that the synthetic compound IM-412 suppressed the EMT process in MDA-MB-231??cells and thereby effectively inhibited the migration and invasion of these cancer cells. Thus, IM-412 could serve as a novel therapeutic agent for malignant cancers.</P>