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- 저자 : ( Du San Baek ) (검색결과 22 건)
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( Du San Baek ),( Yong Sung Kim ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.3
Yeast surface-displayed antibody libraries provide an efficient and quantitative screening resource for given antigens, but suffer from typically modest library sizes owing to low yeast transformation efficiency. Yeast mating is an attractive method for overcoming the limit of yeast transformation to construct a large, combinatorial antibody library, but the optimal conditions have not been reported. Here, we report a large synthetic human Fab (antigen binding fragment) yeast surface-displayed library generated by stepwise optimization of yeast mating conditions. We first constructed HC (heavy chain) and LC (light chain) libraries, where all of the six CDRs (complementarity-determining regions) of the variable domains were diversified mimicking the human germline antibody repertoires by degenerate codons, onto single frameworks of VH3-23 and Vκ1-16 germline sequences, in two haploid cells of opposite mating types. Yeast mating conditions were optimized in the order of cell density, media pH, and cell growth phase, yielding a mating efficiency of ~58% between the two haploid cells carrying HC and LC libraries. We constructed two combinatorial Fab libraries with CDR-H3 of 9 or 11 residues in length with colony diversities of more than 109 by one round of yeast mating between the two haploid HC and LC libraries, with modest diversity sizes of ~107. The synthetic human Fab yeast-displayed libraries exhibited relative amino acid compositions in each position of the six CDRs that were very similar to those of the designed repertoires, suggesting that they are a promising source for human Fab antibody screening.
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Du San Baek,Sang Hoon Joo 대한화학회 2022 Bulletin of the Korean Chemical Society Vol.43 No.10
Since the pioneering work of Ryoo and co-workers in 1999 on the synthesis ofordered mesoporous carbons via nanocasting, the nanocasting pathway hasendured the test of time and has established itself as a robust and versatilemethod for preparing ordered mesoporous materials (OMMs). This methodenables the generation of a remarkably wide range of non-siliceous OMMs,some of which are otherwise inaccessible. In this account, we present ourdecade-long efforts toward the function-oriented design of non-siliceousOMM-based electrocatalysts for promoting small molecule conversion reac-tions relevant to renewable energy conversion and commodity chemical pro-duction, including hydrogen evolution reaction, oxygen evolution reaction, andselective oxygen reduction reaction. We also discuss the extension of the nano-casting concept to a silica-coating-mediated synthesis for preparing new cata-lytic materials. We conclude the account by suggesting the remainingchallenges and outlook for the future development of advanced OMMs.
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Baek, Du-San,Kim, Jeong-Ho,Kim, Ye-Jin,Kim, Yong-Sung American Chemical Society 2018 MOLECULAR PHARMACEUTICS Vol.15 No.2
<P>Neuropilin-1 (NRP1), which functions as a coreceptor for vascular endothelial growth factor (VEGF) and is implicated in vascular permeability and tumorigenesis, has been targeted by peptides that specifically bind to the VEGF-binding region on NRP1. Like natural VEGF ligands, all known peptides with NRP1-binding activity bind only through a carboxy (C)-terminal R/K-x-x-R/K sequence motif (x stands for any amino acids); this strict requirement is called the C-end rule (CendR). Here, we report immunoglobulin Fc-fused NRP1-specific peptides deviating from CendR. We screened a yeast surface-displayed Fc-fused non-CendR peptide library against NRP1 and isolated Fc-V12, wherein V12 peptide comprising 12 amino acids has a PPRV sequence at its C-terminal end. Although Fc-V12 lacked the CendR motif, it showed selective binding to the VEGF-binding region of NRP1 and triggered cellular internalization of NRP1, which resulted in enhanced extravasation into tumor tissues and tumor tissue penetration of the Fc-fused peptide along with the coinjected chemical drug in tumor-bearing mice. Through a saturation mutagenesis study, we identified that the Val residue at the C-terminus of Fc-V12 is crucial for NRP1 binding. We further improved NRP1 affinity of Fc-V12 (<I>K</I><SUB>D</SUB> = ∼761 nM) through directed evolution of the upstream sequence of PPRV to obtain Fc-V12–33 (<I>K</I><SUB>D</SUB> = ∼17.4 nM), which exhibited enhanced NRP1-mediated vascular permeability as compared with Fc-V12. Our results provide functional Fc-fused non-CendR peptides, which bind to the VEGF-binding region of NRP1 and enhance vascular permeability, expanding the sequence space of NRP1-targeting peptides.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/mpohbp/2018/mpohbp.2018.15.issue-2/acs.molpharmaceut.7b00761/production/images/medium/mp-2017-007613_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/mp7b00761'>ACS Electronic Supporting Info</A></P>
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백두산(Du-San Baek),양윤상(Yoon-sang Yang),이동훈(Dong-Hoon LEE),박춘근(Chun-Geun Park) 한국철도학회 2014 한국철도학회 학술발표대회논문집 Vol.2014 No.10
지하철 터널과 고속철도 터널 및 자동차 전용도로에 건설된 터널과 같은 경우는 반폐쇄적인 공간적 특성으로 인하여 음에너지 밀도가 매우 높은 반사음장이 형성되어 강한 세기의 음파가 터널 입구와 출구 방향으로 전파되어 터널 입출구 근방에서 또 다른 소음문제를 야기시킨다. 그러나 지금까지는 터널내부에서의 소음저감을 다룬 연구가 대부분이었으며[1~3], 터널 입구와 출구에서의 소음저감 대책이나 소음전파 특성에 대한 연구결과가 발표된 사례는 거의 없었다. 따라서 본 연구에서는 터널 입구와 출구방향으로 방사되는 소음을 저감시키기 위한 일환으로 관 출구를 경사지게 하거나 관 출구에 다양한 크기의 플랜지를 부착하면서 소음전파 특성을 측정하고 음향해석 결과와 비교하였다. In the tunnel of rail way or motorcar road, reflection sound field with high density sound energy has been generated and radiated to entrance, And then it leads to another noise problem around tunnel entrance. But most of researches were conducted about noise reduction at inside of tunnel and the study on the noise reduction countermeasure or noise propagation characteristics at entrance of tunnel has scarcely been performed. Therefore, to reduce the radiated noise from tunnel exit we measured both noise directivity and reflection coefficient and compared those with simulation results by changing the conditions of inclined exit or flange attached to exit.
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관 경사출구에 부착된 플랜지가 음향반사계수에 미치는 영향
백두산(Du-San Baek),양윤상(Yoon-Sang Yang),이동훈(Dong-Hoon Lee),조재형(Jae-Hyeong Jo) 한국소음진동공학회 2014 한국소음진동공학회 학술대회논문집 Vol.2014 No.10
This research is to review the possibility of reducing the noise radiated from the tube exit by controling the sound reflection coefficient at the inclined exit. The sound reflection coefficient at the inclined exit of flanged tube was measured by both transfer function method and standing wave ratio method. Accuracy on the sound reflection coefficient measured by transfer function method was verified through comparison with sound reflection coefficient measured by standing ratio method. The flanged tube had lower sound reflection coefficient than the tube which have no flange. Also the sound reflection coefficient was decreased in accordance with increasing the inclined angle of unflanged tube.
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