Downregulation of FoxM1 sensitizes nasopharyngeal carcinoma cells to cisplatin via inhibition of MRN-ATM-mediated DNA repair

( Dandan Li ),( Lin Ye ),( Yue Lei ),( Jie Wan ),( Hongyan Chen ) 생화학분자생물학회 2019 BMB Reports Vol.52 No.3

Chemoresistance is the primary obstacle in the treatment of locally advanced and metastatic nasopharyngeal carcinoma (NPC). Recent evidence suggests that the transcription factor forkhead box M1 (FoxM1) is involved in chemoresistance. Our group previously confirmed that FoxM1 is overexpressed in NPC. In this study, we investigated the role of FoxM1 in cisplatin resistance of the cell lines 5-8F and HONE-1 and explored its possible mechanism. Our results showed that FoxM1 and NBS1 were both overexpressed in NPC tissues based on data from the GSE cohort (GSE12452). Then, we measured FoxM1 levels in NPC cells and found FoxM1 was overexpressed in NPC cell lines and could be stimulated by cisplatin. MTT and clonogenic assays, flow cytometry, γH2AX immunofluorescence, qRT-PCR, and western blotting revealed that downregulation of FoxM1 sensitized NPC cells to cisplatin and reduced the repair of cisplatin-induced DNA double-strand breaks via inhibition of the MRN (MRE11-RAD50-NBS1)-ATM axis, which might be related to the ability of FoxM1 to regulate NBS1. Subsequently, we demonstrated that enhanced sensitivity of FoxM1 knockdown cells could be reduced by overexpression of NBS1. Taken together, our data demonstrate that downregulation of FoxM1 could improve the sensitivity of NPC cells to cisplatin through inhibition of MRN-ATM-mediated DNA repair, which could be related to FoxM1-dependent regulation of NBS1. [BMB Reports 2019; 52(3): 208-213]

Granularity and Laxative Effect of Ultrafine Powder of Dendrobium officinale

DanDan Luo,Chao Qu,ZhenBiao Zhang,JianHui Xie,LieQiang Xu,HongMei Yang,CaiLan Li,GuoSheng Lin,HongFeng Wang,ZiRen Su 한국식품영양과학회 2017 Journal of medicinal food Vol.20 No.2

Constipation is a common disorder that is a significant source of morbidity among people around the world ranging from 2% to 28%. Dendrobium officinale Kimura et Migo is a traditional herbal medicine and health food used for tonicity of the stomach and promotion of body fluid production in China. This study aimed to prepare the ultrafine powder of Dendrobium officinale (UDO) and investigate its laxative effect and potential mechanism in mice with diphenoxylate-induced constipation. Results indicated that the mean diameter (d50) of UDO obtained by ball milling was 6.56 lm. UDO (62.5, 125, and 250 mg/kg, p.o.) could significantly enhance the gastrointestinal transit ratio and promote fecal output. Moreover, UDO treatment resulted in significant increases in the serum levels of acetylcholinesterase (AChE), gastrin (Gas), motilin (MTL), and substance P (SP), and obviously decreased serum contents of somatostatin (SS). Taken together, UDO, which can be easily obtained through milling to a satisfactory particle size, exhibited obvious laxative effect in diphenoxylate-induced constipated mice, and the mechanism might be associated with elevated levels of AChE, Gas, MTL, SP, and reduced production of SS. UDO has the potential for further development into an alternative effective diet therapy for constipation.

Protein Associated with Adventitious Root Induction Analysis of Tree Peony (Paeonia suffruticosa Andr.) Plantlets In Vitro by Two-dimensional Electrophoresis and Mass Spectrometry

Dandan Zhang,Zheng Wang,Liyun Shi,Wenqian Shang,Zhenzhu Fu,Dan He,Song Lin He 한국화훼학회 2016 화훼연구 Vol.24 No.2

In the present study, the plantlets in vitro of Paeonia suffruticosa ‘Wu Long Peng Sheng’ were used as laboratory materials. The proteome during adventitious root induction process was investigated to sift the related proteins by two-dimensional electrophoresis and mass spectrometry. The results indicated that the protein spots were concentrated in the acidity gel region (pH 4 - 7) and the spots number had a dynamic change ranged from 373 to 462 at the process of root induction (0 – 7 d). 8 spots significantly changed were analyzed with a mass spectrometer and identified using associated software and databases. The peptide information of the 8 spots was similar to the ATP synthase β-subunit of P. suffruticosa (Spots 1 - 4 and 8), P. tenuifolia (Spots 5), P. californica (Spot 6) and P. brownie ( Spots 7) r espectiv ely. T he expression levels of protein spots 1, 4, 5, 6 and 7 was dramatically downregulated, and that of protein spots 2 and 3 had a slightly opposite tendency on the 3rd day. The obviously decreased period is particularly interesting as it was consistent with the induction period of adventitious root primordial of tree peony plantlet in vitro. The ATP synthase β-subunit could be consumed for assembling the ATP synthase in order to supply energy to the rooting process. Therefore, we speculated that the ATP synthase β-subunit was involved in adventitious root initiation of tree peony plantlets in vitro and we expect that further studies should be carried out in order to export its action mechanism.

TEOA, a triterpenoid from Actinidia eriantha, induces autophagy in SW620 cells via endoplasmic reticulum stress and ROS-dependent mitophagy

Dandan Zhang,Cuixia Gao,Ruyi Li,Lin Zhang,Jingkui Tian 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.5

2a,3a,24-Thrihydroxyurs-12-en-28-oicacid (TEOA),a pentacyclic triterpenoid, isolated from the roots of Actinidiaeriantha, exhibits significant cytotoxicity against SW620, BGC-823, HepG-2, A549 and PC-3 cancer cells. In this study, weinvestigated the underlying molecular mechanism of the anticanceractivity of TEOA in SW620 cells.We demonstrated thatTEOA induced apoptosis through cleavage of caspase-9 andPARP in SW620 cells. In addition, evidence of TEOA-mediatedautophagy included the induction of autophagolysosomesand activation of autophagic markers LC-3B and p62. Furtheranalysis illustrated that TEOA promoted the phosphorylation ofPERK and elF2a, followed by up-regulation of the downstreamprotein CHOP, suggesting the involvement of PERK/eIF2a/CHOP pathway and ER stress in TEOA-induced autophagy inSW620 cells. Meanwhile, TEOA-mediated PINK1, Parkin,ubiquitin and p62 activation revealed that TEOA inducedspecific autophagy-mitophagy in SW620 cells. Additionally, anantioxidant NAC attenuated the TEOA-induced mitophagy,indicating that TEOA triggers mitophagy via a ROS-dependentpathway. Collectively, our findings revealed a novel cellularmechanism of TEOA in the colon cancer cell line SW620, thusproviding a molecular basis for developing TEOA into an antitumorcandidate.

An S-scheme photocatalyst constructed by modifying Ni-doped Sn3O4 micro-flowers on g-C3N4 nanosheets for enhanced visible-light-driven hydrogen evolution

Dandan Wang,Zhaoxin Lin,Chun Miao,Wei Jiang,Hongji Li,Chunbo Liu,Guangbo Che 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.113 No.-

Carbon nitrides (g-C3N4) is considered to be the prospective semiconductor photocatalyst for photocatalytic H2 evolution. Nevertheless, it suffers from low charge transfer efficiency and fewer metal active sites. Thereby, Ni-Sn3O4/g-C3N4 photocatalysts were constructed by anchoring Ni-doped Sn3O4 micro-flowers on g-C3N4 via a feasible and straightforward solvothermal treatment. The prepared Ni-Sn3O4/g-C3N4 S-scheme heterojunction could improve the transfer and separation efficiency of photo-generated electron-hole pairs by facilitating the electrons transfer from Ni-Sn3O4 to g-C3N4. Moreover, the photocatalytic H2 production performance was ameliorated due to the established internal electric field and the energy band bending in Ni-Sn3O4/g-C3N4 S-scheme heterojunction. Meanwhile, the doping Ni in Sn3O4 exposed more active sites in Ni-Sn3O4/g-C3N4 heterojunction for producing H2. As a result, Ni-Sn3O4/g-C3N4-5 photocatalyst exhibited outstanding H2 yields of 1961 µmol h−1 g−1 under visible light irradiation in comparison with pure Ni-Sn3O4 (12 µmol h−1 g−1) and bared g-C3N4 (1391 µmol h−1 g−1). Furthermore, the S-scheme mechanism in Ni-Sn3O4/g-C3N4 heterojunction for producing H2 by oxidizing H2O was proposed. This study provides helpful guide for developing efficient g-C3N4-based photocatalytic systems.

Metallothionein MT1M Suppresses Carcinogenesis of Esophageal Carcinoma Cells through Inhibition of the Epithelial-Mesenchymal Transition and the SOD1/PI3K Axis

Li, Dandan,Peng, Weiyan,Wu, Bin,Liu, Huan,Zhang, Ruizhen,Zhou, Ruiqin,Yao, Lijun,Ye, Lin Korean Society for Molecular and Cellular Biology 2021 Molecules and cells Vol.44 No.4

Metallothionein (MT1M) belongs to a family of cysteinerich cytosolic protein and has been reported to be a tumor suppressor gene in multiple cancers. However, its role in esophageal carcinoma carcinogenesis remains unclear. In this study, MT1M expression was correlated with tumor type, stage, drinking and smoking history, as well as patient survival. We also studied the regulation and biological function of MT1M in esophageal squamous cell carcinoma (ESCC). We have found that MT1M is significantly downregulated in ESCC tissues compared with adjacent non-cancer tissues. Furthermore, restoration of expression by treatment with the demethylation agent A + T showed that MT1M downregulation might be closely related to hypermethylation in its promoter region. Over-expression of MT1M in ESCC cells significantly altered cell morphology, induced apoptosis, and reduced colony formation, cell viability, migration and epithelial-mesenchymal transition. Moreover, based on reactive oxygen species (ROS) levels, a superoxide dismutase 1 (SOD1) activity assay and protein analysis, we verified that the tumor-suppressive function of MT1M was at least partially caused by its upregulation of ROS levels, downregulation of SOD1 activity and phosphorylation of the SOD1 downstream pathway PI3K/AKT. In conclusion, our results demonstrated that MT1M was a novel tumor-suppressor in ESCC and may be disrupted by promoter CpG methylation during esophageal carcinogenesis.

Effects of Selenizing Codonopsis pilosula Polysaccharide on Macrophage Modulatory Activities

( Tao Qin ),( Zhe Ren ),( Dandan Lin ),( Yulong Song ),( Jian Li ),( Yufang Ma ),( Xuehan Hou ),( Yifan Huang ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.8

The purpose of the present study was to investigate the immune-enhancing activity of selenizing Codonopsis pilosula polysaccharide (sCPPS5) in nonspecific immune response. In in vitro experiment, the results showed that sCPPS5 could promote the phagocytic uptake, NO production, and TNF-α and IL-6 secretion of RAW264.7 cells. sCPPS5 could also strongly increase the IκB-α degradation in the cytosol and the translocation of NF-κB p65 subunit into the nucleus of RAW264.7 cells. In the vivo experiment, sCPPS5 at medium doses could significantly improve the phagocytic index of peritoneal macrophages and induce the secretion of TNF-α and IL-6. Moreover, the effect of sCPPS5 was significantly better than Codonopsis pilosula polysaccharide (CPPS). These results indicated that selenylation modification could significantly enhance the immune-enhancing activity of CPPS in the nonspecific immune response.

Association between Delayed Lactogenesis II and Early Milk Volume among Mothers of Preterm Infants

Xiurong Yu,Jianhua Li,Xiangyun Lin,Dandan Luan 한국간호과학회 2019 Asian Nursing Research Vol.13 No.2

Purpose: This study aimed to evaluate the effect of delayed lactogenesis Ⅱ on early milk volume in mothers expressing milk for their preterm infants. Methods: 142 mothers with preterm infants participated in a longitudinal cohort study, the milk volumes over 14 days postpartum between mothers with delayed lactogenesis Ⅱ ( 72 hours) and mothers with non-delayed lactogenesis Ⅱ(< 72 hours) were compared using Wilcoxon's rank sum tests. Results: The prevalence of delayed lactogenesisⅡ among mothers of preterm infants was 36.0% (36/100). There existed negative correlations between the onset of lactogenesis Ⅱ and the daily milk volumes( rs ¼ 0.525~0.354, p ¼ .002 ~ p < .001). The milk volumes in every 24-hour of the 14 days postpartum in delayed group were significantly less than that in non-delayed group (p ¼ .002 ~ p < .001). After controlling for the covariates, pregnancy-induced hypertension syndrome, delayed expression initiation, shorter daily sleeping time were found to be the risk factors for delayed lactogenesis Ⅱ. Conclusion: Delayed lactogenesis Ⅱ was associated with lower milk volume in early postpartum period. Women who were at risk for delayed lactogenesis Ⅱ need targeted interventions and additional support during pregnancy and postpartum.