Hypoxia-Responsive MicroRNA-101 Promotes Angiogenesis <i>via</i> Heme Oxygenase-1/Vascular Endothelial Growth Factor Axis by Targeting Cullin 3

Kim, Ji-Hee,Lee, Kwang-Soon,Lee, Dong-Keon,Kim, Joohwan,Kwak, Su-Nam,Ha, Kwon-Soo,Choe, Jongseon,Won, Moo-Ho,Cho, Byung-Ryul,Jeoung, Dooil,Lee, Hansoo,Kwon, Young-Guen,Kim, Young-Myeong Mary Ann Liebert 2014 Antioxidants & redox signaling Vol.21 No.18

<P>Aims: Hypoxia induces expression of various genes and microRNAs (miRs) that regulate angiogenesis and vascular function. In this study, we investigated a new functional role of new hypoxia-responsive miR-101 in angiogenesis and its underlying mechanism for regulating heme oxygenase-1 (HO-1) and vascular endothelial growth factor (VEGF) expression. Results: We found that hypoxia induced miR-101, which binds to the 3 ' untranslated region of cullin 3 (Cul3) and stabilizes nuclear factor erythroid-derived 2-related factor 2 (Nrf2) via inhibition of the proteasomal degradation pathway. miR-101 overexpression promoted Nrf2 nuclear accumulation, which was accompanied with increases in HO-1 induction, VEGF expression, and endothelial nitric oxide synthase (eNOS)-derived nitric oxide (NO) production. The elevated NO-induced S-nitrosylation of Kelch-like ECH-associated protein 1 and subsequent induction of Nrf2-dependent HO-1 lead to further elevation of VEGF production via a positive feedback loop between the Nrf2/HO-1 and VEGF/eNOS axes. Moreover, miR-101 promoted angiogenic signals and angiogenesis both in vitro and in vivo, and these events were attenuated by inhibiting the biological activity of HO-1, VEGF, or eNOS. Moreover, these effects were also observed in aortic rings from HO-1(+/-) and eNOS(-/-) mice. Local overexpression of miR-101 improved therapeutic angiogenesis and perfusion recovery in the ischemic mouse hindlimb, whereas antagomiR-101 diminished regional blood flow. Innovation: Hypoxia-responsive miR-101 stimulates angiogenesis by activating the HO-1/VEGF/eNOS axis via Cul3 targeting. Thus, miR-101 is a novel angiomir. Conclusion: Our results provide new mechanistic insights into a functional role of miR-101 as a potential therapeutic target in angiogenesis and vascular remodeling. Antioxid. Redox Signal. 21, 2469-2482.</P>

심근 허혈-재관류 백서 모델에서 mitogen-activated protein kinase 활성화에 대한 α-lipoic acid의 효과

김연경,박은미,최준호,윤경호,유남진,이은미,김남호,정진원,김민선,박병림,오석규 圓光大學校 醫科學硏究所 2006 圓光醫科學 Vol.21 No.1

형성평가에 대한 과학교사들의 인식 및 실태

南貞姬,成乙善,飮在鎬,金慶姬,崔秉舜 한국교원대학교 과학교육연구소 1999 청람과학교육연구논총 Vol.9 No.1

전국의 초, 중, 고등학교 과학교사 197명을 대상으로 과학교육에서 형성평가에 대한 인식 및 이에 대한 실태를 질문지를 이용하여 조사하였다. 교사들은 학교교육에서 평가의 가장 중요한 목적이 학습목표의 성취를 확인하기 위한 것이라고 생각하고 있었으며, 가장 적절한 평가방법은 형성평가라고 생각하고 있었다. 따라서 형성평가는 수업 후반부에 학습내용의 성취정도를 확인하는 평가라고 인식하고 있었다. 대부분의 교사들이 형성평가가 매우 필요하다고 인식하고 있었고, 그 이유로 학습목표 달성 정도의 확인을 들었다. 그러나 과학 수업 중 수시로 이루어지는 형성평가는 적었다. 형성평가의 실시를 어렵게 만드는 원인으로 학생 수 과다, 시간 부족, 준비의 어려움을 들었으며, 형성평가로부터 얻은 정보는 학생들의 성취수준을 판단하는데 이용한다고 응답하였다. The science teachers' perceptions and the status of formative assessment in science teaching were identified through a questionnaire. The majority of teachers thought that the most important goal of assessment in education was to identify to what extent pupils achieved learning objectives. They suggested that formative assessment was the most appropriate method regardless of the purposes of assessment. They thought that formative assessment was an assessment carried out at the end of lesson to identify to what extent pupils achieved learning objectives. The teachers stressed the need for formative assessment to identify what pupils achieved. But they carried out formative assessment about once a week and there were very few teachers carrying out formative assessment during the lesson. They responded that it was difficult to carry out formative assessment because of large class size, lack of time and difficulty in preparation. And they used the information from formative assessment to judge the level of attainment.

복합레진의 중합 수축력과 수복물의 결합양상에 관한 연구 제 1 편 : 중합수축력

박남수,최대균,임호남,최부병,우이형 大韓齒科器材學會 1993 대한치과재료학회지 Vol.20 No.1

The polymerization contraction forces has a relationship with the contents of filler, and if forces surpass the bond strength between restoration and bonded surface, the failure of bond can be occured. This study was designed to evaluate the effects of filler contents on the polymerization contraction forces and to confirm whether the polymerization forces could induce the bond failure of composite resins to enamel or dentin. Low capacity load cell using strain gauge were prepared and used as a measurement system for polymerization contraction forces. Oxidized alloy surfaces, enamel surfaces and dentin surfaces were used as bonded surface. Measurements of the polymerization contraction forces were done for 1 hour, and a stress at 1 hour after was recorded as a polymerization contraction force. It was considered as a bond failure that the polymerization contraction forces increased and then decreased by the time. The following conclusions were drawn : 1. Polymerization contraction forces of composite resins were decreased by the increase of filler contents. 2. Polymerization contraction forces were increased at thickened resin specimens, but it was not significant at over two millimeter thickness. 3. Bond strength of composite resins to the acid etched enamel surfaces exceeded the polymerization contraction forces and the reduced polymerization contraction forces due to the bond failure were not confirmed. 4. Failure of bond and reduced polymerization contraction forces occured on dentin.

學敎給食이 學生의 體格, 體力 및 體質에 미치는 影響

甘炳浩,申重圭,南喆鉉 韓國學校保健學會 1992 韓國學校保健學會誌 Vol.5 No.1

The study examined the difference of the physique, the physical strength, and the physical constitution between meal and non-meal service schools at the countryside, and concluded as follows : 1) The effects on the physical development Meal service school student's height was 0.41 ~ 2.38 cm taller, weight 0.21 ~ 1.58 kg heavier, and the chests 0.15 ~ 2.55 cm bigger than the non-meal service school students. Therefore, the effects of the school meal service on the student's physique development proved positive. 2) The effects on the physical strengh Today, the student's physical growth is reinforced by consuming high calorie food, while their physical exercise is decreased. As a result, obesity becomes another problem. In this respect, physical strength is a significant factor in judging the physical condition of the students. This study compared the meal service schools with the non-meal service schools by suing six items of the physical strength test those schools. In comparison, the meal service school students were better than the others in most items except in the eleven year old girl's 100 meter running and the ball throwing, and the ten year old boy's 600 meter running. It means that school meal service affects the physical growth in a positive manner. 3) The effects on the physical constitution The students in their growing period should have their meals not only balanced, but regularly since this helps to maintain their health. Insufficient nutrition may disturb the balanced physical growth of cause a week or powerless physical constitution. As shown at the table 6, meal service school students who had a balanced meal have 0.01% lower incidence of disease, and 3.11 ~ 7.32 % less myophia than the other. The average 0.39 to 0.63 cavity of the meal service school students also show that their constitutions are better than the non-meal service ones. In conclusion, the school meal service program gives every student a regular nutritious meal which results in a balanced physical growth. Erentually, the healthier students could be the healthier social menbers which promise a brighter society in the future.

영광 원자력발전소 6호기 가동중검사 수행 경험

김영호,남민우,양승한,윤병식,김용식 한국비파괴검사학회 2004 한국비파괴검사학회지 Vol.24 No.4

원자력발전소 운전에 따른 경년열화 등에 의하여 원자력발전소 주요 기기 및 재료 등에 손상 발생 가능성이 있어 원자력법 및 관련 기술기준에서는 비파괴검사 방법을 이용하여 원자력발전소 주요 기기 및 배관의 용접부등 취약 부위에 대한 건전성을 주기적으로 펑가토록하고 있다. 이에 따라, 영광 6호기 가동중검사는 기기, 배관 및 구조물 비파괴검사, 압력용기 자동 초음파탐상검사, 원자로 내부 구조물 육안검사 및 증기발생기 전열관 와전류탐상검사로 구분하여 수행하였다. 원자력발전소 계통의 주요기기에 대한 비파괴검사 결과, 기기, 배관 및 구조물과 원자로 압력용기 용접부에 대해서는 특이 사항 발생 없이 적용 규격에 만족되고 건전한 것으로 최종 평가되었다. 특히, 배고나 용접부에 대한 초음파탐상검사는 영광 5호기에서와 마찬가지로 ASME Code Sec. IX 1995년도 판에 따라 기량검증(Performance Demonstration : PD) 방법을 적용함으로써 검사 신뢰도를 확보하였다는데 큰 의미가 있다. As the increase of the operation year of nuclear power plants, the probabilities of the degradation of the major facilities and materials in the nuclear power plants are increased. The integrity of those facilities shall be monitored and verified by the non-destructive examination methods with the regulation codes, so called inservice inspection(ISI). The ISI of Yonggwang unit 6 was performed in four different parts, 1)non-destructive examinations for the components, piping weldments and structures, 2)automated ultrasonic examinations for pressure vessels, 3)visual examinations for the interior structures of the reactor, 4)eddy current examinations for the steam generator tubes. As the results, there was no severe indication and all detected indications were evaluated as non-relevant. Especially for the examinations of the piping weldments, PD(Performance Demonstration) was applied as a UT examination method defined in the 1995 edition of ASME Code Sec. XI. The implementation of the PD for the piping weld results in an improvement of the reliability of the UT examinations.

감압술을 이용한 치근단 낭의 처치

김남혁,최병재,이제호,손흥규,김성오,최형준 大韓小兒齒科學會 2009 大韓小兒齒科學會誌 Vol.36 No.2

치근단 낭은 염증 등에 의한 자극으로 치수가 실활되어 치근단에 있는 상피로부터 형성되는 낭종으로 주된 기원은 말라세즈 상피잔사이지만 치은열구 상피나 상악동 이장 상피, 누공의 상피에 의한 것도 보고 되었다. 구강악안면 영역에서 가장 흔히 발생되는 낭종의 하나이지만 유치열기에서의 발생 빈도는 낮은 것으로 알려져 있다. 악골 내의 치성 낭종은 크기가 커지면서 주위 조직의 파괴나 구조의 변형을 초래할 수 있으며 유치의 경우에서는 후속 영구치배의 변위를 유발할 수 있다. 이런 큰 크기의 치성 낭종을 외과적으로 제거하면 해부학적 구조의 손상 및 안모의 변형, 치아흡수, 신경손상 등의 후유증을 일으킬 수 있으므로 조대술이나 감압술 후 적출하는 술식이 권장되고 있다. 이 증례는 하악 우측 부위의 동통을 주소로 연세대학교 치과대학병원 소아치과에 내원한 8세 남아로, 임상 및 방사선학적검사 결과 치수치료 후 기성금속관의 수복을 시행한 하악 우측 제1유구치의 치근단 하방에 방사선투과성 병변과 견치와 제1소구치의 변위를 발견하였다. 병소가 있는 하악 우측 제1유구치를 발거하였고, 조직학적 검사에서 치근단 낭으로 진단되었다. 감압술 및 공간유지장치를 시행하고 병소의 크기 변화와 하악 우측 견치와 제1소구치의 맹출 양상을 정기적으로 관찰한 결과 양호한 치유 양상과 정상적인 맹출을 보였기에 이를 보고하고자 한다. Radicular cyst is a true epithelium lined cyst formed when epithelium at the apex of a nonvital tooth is stimulated by inflammation. It is the most common type of cyst in the oral cavity, but its frequency is low in the primary dentition. Treatment of large-sized cyst aims at conservation of adjacent structure and allowance of proper eruption of the successive permanent teeth in the primary dentition. Considering these two aspects, marsupialization or enucleation following decompression is recommended as a treatment means for large-sized radicular cyst. In this case, 8-year old boy visited the pediatric dentistry department, Yonsei University Dental Hospital, with the chief complaint of pain on the lower right area. Clinical and radiographic examinations revealed periapical radiolucent lesion on #84, which had previous pulp treatment and restorated with the stainless steel crown. Dislocation of its successive tooth, #44, was also observed. #84 was extracted and sent for biopsy. Through histologic examination, it was diagnosed as radicular cyst. Following its extraction, removable space maintainer was delivered, which was also used as a decompressor. During periodic check-up for next 22 months, favorable healing of the lesion and eruption of the successive tooth were observed.

이식형 인공중이 시스템을 위한 고효율 차동 전자 트랜스듀서의 설계

송병섭,노철균,김명남,조진호 한국센서학회 2002 센서학회지 Vol.11 No.3

이식형 인공중이에 사용되는 일반적인 전자기 방식의 진동 트랜스듀서에 비하여 두 개의 소형 영구자석을 같은 극끼리 접착한 후 코일내부에 위치시킨 차동 전자 트랜스듀서는 효율이 높고 코일-영구자석간의 공극조정이 필요 없으며 외부의 자기장에 대하여 영향을 받지 않는 특성을 가진다. 본 논문에서는 이러한 차동 전자 트랜스듀서를 이식형 인공중이 시스템에 사용하기 위하여 입력전류에 대하여 진동력의 크기를 최대화시킬 수 있는 효율적인 구조를 제안 해석하였다. 유한요소 해석을 통하여 가장 효율적인 코일과 자석의 길이 비를 도출하였고 소리신호 전달을 위한 트랜스듀서의 필요 진동력을 산출한 후 트랜스듀서 발생 진동력을 해석하였다. 그리고 실제 이소골에 이식할 수 있는 크기의 트랜스듀서를 제작하기 위한 설계상수를 추출하였다. 계산된 설계상수를 이용하여 이식 가능한 크기로 트랜스듀서를 구현한 후 무부하 실험과 사체 부하 실험을 실시하여 제작된 트랜스듀서가 실제 중등이상 고도난청자를 위한 이식형 인공중이 시스템에 사용될 수 있음을 증명하였다. The differential electromagnetic transducer for IME(implantable middle ear) system, which have two small permanent magnets glued with the same pole facing each other in the coil, has high vibration efficiency and does not influenced by external magnetic field. In this paper, using finite element method, highly efficient structure of the transducer was proposed and vibration force of the transducer was calculated by electromagnetic theory. And the necessary vibration force of transducer to transmit the sound signal to inner ear when the transducer is attached at stapes was calculated and the design parameters of the transducer were investigated. Using the parameters, the differential electromagnetic transducer was manufactured in small size to implant in confined human middle ear. And it was examined by unloaded and loaded vibration experiment using temporal bone sampled from cadaver.

복합레진의 중합 수축력과 수복물의 결합양상에 관한 연구 제 2 편 결합강도

박남수,우이형,최부병,임호남,최대균 大韓齒科器材學會 1993 대한치과재료학회지 Vol.20 No.2

Failure of bond between resinous restorative materials and tooth can be occured by the polymerization contraction forces larger than bond strengths. This study was planned to confirmed that the bond strengths between composite resin and acid etched enamel will be influenced by the polymerization contraction forces altered with filler contents and diameter of attached resin specimens. Ten kinds of experimental composite resins were prepared and bonded to bovine tooth enamel which were acid-etched. The diameter of resin specimens attached were 4 mm and 6 mm. The following conclusions were drawn : 1. Shear bond strengths were increased and then decreased with rise of filler contents, maximum shear bond strength was obtained by the 40% filled composite resin. 2. Unit shear bond strengths of resin specimens bonded with large area were lower than that of narrow area bonding. It was concluded that shear bond strengths to acid etched enamel can be influenced by the polymerization contraction forces.

매복된 상악 중절치의 교정적 처치를 통한 치험례

김남혁,김성오,송제선,손흥규,최병재,이제호,최형준 大韓小兒齒科學會 2010 大韓小兒齒科學會誌 Vol.37 No.1

매복은 임상 검사나 방사선 사진 검사 상에서 인식 가능한 맹출 경로 상에 장애물이나 이소 위치에 의한 치아 맹출의 정지를 의미한다. 상악 중절치의 치아 매복의 원인으로는 과잉치, 치아종, 치배의 이소위치, 외상 후 연조직 치유에 의한 폐쇄, 만곡치, 정지된 치근 발육 등이 있다. 매복된 치아는 인접치의 근심이동으로 인한 공간 상실, 부정교합, 인접치의 치근 흡수, 낭종의 형성 등을 유발할 수 있으므로 맹출장애에 대한 주의 깊은 관찰과 조기 진단이 중요하며 적절한 치료가 시행되어야 한다. 매복된 치아의 치료방법으로는 유치의 발거나 외과적 노출을 통한 맹출 유도, 경과관찰, 외과적 방법이나 교정력을 이용한 매복치의 재위치, 매복치의 외과적 발거 등이 있다. 이 중 교정적 견인은 매복된 치아가 원인을 제거한 후 또는 외과적으로 치관을 노출시킨 후에 일정기간의 관찰에도 맹출하지 않거나, 맹출경로가 정상에서 많이 벗어나 맹출이 기대되지 않을 경우 적용될 수 있다. 본 증례는 매복된 상악중절치를 교정적 견인을 통해 정상적인 맹출 방향으로 유도한 치험례들로 양호한 치료 결과를 보였기에 이를 보고하고자 하는 바이다. Impaction is defined as a cessation of the eruption of a tooth caused by a clinically or radiographically detectable physical barrier in the eruption path or by an ectopic position of the tooth. The reasons for impaction of the maxillary central incisor are supernumerary tooth, odontoma, ectopic position of tooth germ, dilacerated tooth and so force. Impacted tooth cause space loss due to proximal movement of adjacent tooth, malocclusion, root resorption of adjacent tooth, cyst formation, so careful observation and early detection is important and exact treatment should be applied to prevent these results. The treatment options of impacted tooth include induction an eruption through extraction of deciduous tooth or surgical exposure, reposition of impacted tooth by surgical method or orthodontic treatment. Orthodontic traction is recommended when an eruption does not happen after removal of barrier or surgical exposure, when eruption path is too transpositioned to be corrected spontaneously so eruption does not expected. In these cases, traction of impacted maxillary central incisor was carried out using orthodontic method with closed eruption technique and it showed good clinical results so we report these cases.