S-580 : Vitamin D and carotid subclinical atherosclerosis in SLE

( Chang Bum Bae ),( Ju Yang Jung ),( Bo Ram Go ),( Hyun Ah Kim ),( Chang Hee Suh ) 대한내과학회 2013 대한내과학회 추계학술대회 Vol.2013 No.1

Vitamin D and carotid subclinical atherosclerosis in SLE Chang-Bum Bae, Ju-Yang Jung, Bo-ram Go, Hyun-Ah Kim, Chang-Hee Suh Department of Rheumatology, Ajou University School of Medicine, Suwon, Korea Introduction Atherosclerosis develops earlier in systemic lupus erythematosus (SLE) and is the important cause of mortality. It has been suggested that vitamin D deficiency contributed cardiovascular disease and immune dysregulation in SLE. Methods One-hundred two female patients with SLE and 52 normal controls (NC) were recruited. The carotid artery intima-media thickness (cIMT) and plague were assessed by B-mode ultrasound at the carotid artery level. Vitamin 25(OH)D3 levels were checked by immunoradiometric assay using the Bio-Line 25(OH)D3-Ria CT kit (Bio-Line S.A., Belgium). Disease activity markers were checked at the time of enrollment. Results The cIMT of SLE is 0.41±0.08mm, which is higher than NC (0.37±0.11 mm, p=0.012). Also, carotid artery plague index (PI) of SLE is higher than NC (0.68±1.39 vs 0.26±0.87, p=0.026). The cIMT was correlated with age (r=0.442, p<0.001), body mass index (r=0.246, p=0.013), waist-hip ratio (r=0.245, p=0.013), SLE disease activity index (r=0.239, p=0.016), taking aspirin (r=0.244, p=0.013), and the carotid artery PI was correlated with renal involvement (r=0.224, p=0.023) in patients with SLE. Vitamin 25(OH)D3 was not showed correlation with cIMT and carotid artery PI. Also, it was not correlated with disease activity markers. Conclusion In patients with SLE, the risk of cardiovascular disease measured by cIMT and carotid artery plaque was higher than NC, which may be derived from systemic inflammation. However, vitamin D levels were not showed significant correlations with carotid subclinical atherosclerosis. It may be not suitable to assess vitamin D as marker of disease activity or subclinical atherosclerosis.

Selective cytotoxic effects on human cancer cell lines of phenolic-rich ethyl-acetate fraction from Rhus verniciflua Stokes.

Kim, Ji Hye,Jung, Chang Hwa,Jang, Bo-Hyoung,Go, Ho Yeon,Park, Jong-Hyeong,Choi, You-Kyung,Hong, Seong Il,Shin, Yong Cheol,Ko, Seong-Gyu Institute for Advanced Research in Asian Science a 2009 The American journal of Chinese medicine Vol.37 No.3

<P>Rhus verniciflua Stokes (RVS) is a plant with a long history of medicinal use in Eastern Asia. RVS has been widely used to treat gastritis, stomach cancer and atherosclerosis. The cytotoxic effects of different solvent fractions from an RVS ethanol extract were measured in 11 human cancer cell lines. The study showed that the ethyl-acetate (EtOAC) fraction was the most cytotoxic. This fraction contains a number of phenolic compounds, and this phenolic-rich EtOAC fraction was particularly effective against gastric and breast cancer cells. A purified phenolic-rich EtOAC fraction (PPEF) had a stronger apoptotic effect on these cells. Liquid chromatography-mass spectrometry (LC-MS) analysis showed that the PPEF contained gallic acid, protocatechuic acid, fisetin, sulfuretin, butein and 8 unknown compounds. There were only small amounts of flavonoids: fisetin, sulfuretin and butein. The results showed that PPEF induces apoptosis only in gastric and breast cancer cell lines, but not in lung, colon or liver cancer cell lines. Therefore, PPEF may have a significant potential as an organ-specific anti-cancer agent.</P>

Experiences Providing Medical Assistance during the Sewol Ferry Disaster Using Traditional Korean Medicine

Kim, Kyeong Han,Jang, Soobin,Lee, Ju Ah,Jang, Bo-Hyoung,Go, Ho-Yeon,Park, Sunju,Jo, Hee-Guen,Lee, Myeong Soo,Ko, Seong-Gyu Hindawi 2017 Evidence-based Complementary and Alternative Medic Vol.2017 No.-

<P><B>Background</B></P><P> This study aimed to investigate medical records using traditional Korean medicine (TKM) in Sewol Ferry disaster in 2014 and further explore the possible role of traditional medicine in disaster situation.</P><P><B> Methods</B></P><P> After Sewol Ferry accident, 3 on-site tents for TKM assistance by the Association of Korean Medicine (AKOM) in Jindo area were installed. The AKOM mobilized volunteer TKM doctors and assistants and dispatched each on-site tent in three shifts within 24 hours. Anyone could use on-site tent without restriction and TKM treatments including herb medicine were administered individually.</P><P><B> Results</B></P><P> The total of 1,860 patients were treated during the periods except for medical assistance on the barge. Most patients were diagnosed in musculoskeletal diseases (66.4%) and respiratory diseases (7.4%) and circulatory diseases (8.4%) followed. The most frequently used herbal medicines were Shuanghe decoction (80 days), Su He Xiang Wan (288 pills), and Wuji powder (73 days).</P><P><B> Conclusions</B></P><P> TKM in medical assistance can be helpful to rescue worker or group life people in open shelter when national disasters occur. Therefore, it is important to construct a rapid respond system using TKM resources based on experience.</P>

지역사회 및 병원 임상검체에서 분리한 대장균의 항생제 내성 양상

Kim, Yong-Hui,Go, Jong-Myeong,Gong, Young-Woo,Oh, Bo-Young,Kim, Jung-Hee,Kim, Hye-Young,Lee, Mi-Yeon,Koh, Yeon-Ja,Hwang, Kyoung-Wha,JeGal, Seung,Lee, Jae-Mann 한국미생물학회 2006 미생물학회지 Vol.42 No.4

본 연구에서는 지역사회 일반인에게 분리된 대장균과 종합병원을 내원한 설사환자에서 분리한 대장균에 대하여 항생제 내성현황을 모니터링 하였다. 2006년 2월$\sim$7월까지 인천의 지역사회 일반인 692명의 대변으로부터 순수하게 분리된 대장균 491 균주에 대해 항생제 내성균을 조사한 결과, tetracycline 내성이 46.6%, ampicillin 내성이 41.1% ticarcillin 내성이 37.9%였다. 또한 3차 의료기관의 설사환자에서 분리된 대장균 120 균주에 대하여 ampicillin 내성이 66.9%, ticareillin 내성이 63.8%, tetracycline 내성이 47.2%였다. $\beta$-lactam 분해효소(extended spectrum $\beta$-lactamase)를 생성하는 대장균은 지역사회 일반인의 경우 0.6%, 설사질환자의 경우4..1%로 각각 분리되었다. We monitored antibiotic resistance of Escherichia coli isolates from healthy volunteers of community and hospital patients from February to July in 2006. From disc diffusion test on 4915 E. coli isolates from healthy volunteers of the community, the resistance rates were as follows; tetracycline resistant, 46.6%; ampicillin resistant, 41.1%; ticarcillin resistant,37.9%. From disc diffusion test on 120 E. coli isolates from hospital patients, the resistance rates were as follows: ampicillin resistant, 66.9%; ticarcillin resistant, 63.8%; tetracycline resistant, 47.2%. Extended spectrum $\beta$-lactamase producing E. coli were isolated 0.6% and 4.1% from healthy volunteers and hospital patients.

Inhibition of Pyruvate Dehydrogenase Kinase 2 Protects Against Hepatic Steatosis Through Modulation of Tricarboxylic Acid Cycle Anaplerosis and Ketogenesis

Go, Younghoon,Jeong, Ji Yun,Jeoung, Nam Ho,Jeon, Jae-Han,Park, Bo-Yoon,Kang, Hyeon-Ji,Ha, Chae-Myeong,Choi, Young-Keun,Lee, Sun Joo,Ham, Hye Jin,Kim, Byung-Gyu,Park, Keun-Gyu,Park, So Young,Lee, Chul- American Diabetes Association 2016 Diabetes Vol.65 No.10

<P>Hepatic steatosis is associated with increased insulin resistance and tricarboxylic acid (TCA) cycle flux, but decreased ketogenesis and pyruvate dehydrogenase complex (PDC) flux. This study examined whether hepatic PDC activation by inhibition of pyruvate dehydrogenase kinase 2 (PDK2) ameliorates these metabolic abnormalities. Wildtype mice fed a high-fat diet exhibited hepatic steatosis, insulin resistance, and increased levels of pyruvate, TCA cycle intermediates, and malonyl-CoA but reduced ketogenesis and PDC activity due to PDK2 induction. Hepatic PDC activation by PDK2 inhibition attenuated hepatic steatosis, improved hepatic insulin sensitivity, reduced hepatic glucose production, increased capacity for beta-oxidation and ketogenesis, and decreased the capacity for lipogenesis. These results were attributed to altered enzymatic capacities and a reduction in TCA anaplerosis that limited the availability of oxaloacetate for the TCA cycle, which promoted ketogenesis. The current study reports that increasing hepatic PDC activity by inhibition of PDK2 ameliorates hepatic steatosis and insulin sensitivity by regulating TCA cycle anaplerosis and ketogenesis. The findings suggest PDK2 is a potential therapeutic target for nonalcoholic fatty liver disease.</P>

3,3′-Diindolylmethane Suppressed Cyprodinil-Induced Epithelial-Mesenchymal Transition and Metastatic-Related Behaviors of Human Endometrial Ishikawa Cells via an Estrogen Receptor-Dependent Pathway

Kim, Bo-Gyoung,Kim, Jin-Wook,Kim, Soo-Min,Go, Ryeo-Eun,Hwang, Kyung-A,Choi, Kyung-Chul MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.1

<P>Cyprodinil (CYP) is a pyrimidine amine fungicide that has been extensively used in agricultural areas. 3,3′-Diindolylmethane (DIM) is a derivative of the dietary phytoestrogen, indole-3-carbinol (I3C), which is derived from cruciferous vegetables and considered to be a cancer-preventive phytonutrient agent. In this study, the effects of CYP and DIM were examined on the cell viability, invasion, and metastasis of human endometrial cancer cells, Ishikawa, via epithelial mesenchymal transition (EMT). CYP increased the level of cell viability of Ishikawa cells compared to DMSO as a control, as did E2. Ishikawa cells lost cell-to-cell contact and obtained a spindle-shaped or fibroblast-like morphology in response to the application of E2 or CYP by the cell morphology assay. In the cell migration and invasion assay, CYP enhanced the ability of migration and invasion of Ishikawa cells, as did E2. E2 and CYP increased the expressions of N-cadherin and Snail proteins, while decreasing the expression of E-cadherin protein as EMT-related markers. In addition, E2 and CYP increased the protein expressions of cathepsin D and MMP-9, metastasis-related markers. Conversely, CYP-induced EMT, cell migration, and invasion were reversed by fulvestrant (ICI 182,780) as an estrogen receptor (ER) antagonist, indicating that CYP exerts estrogenic activity by mediating these processes via an ER-dependent pathway. Similar to ICI 182,780, DIM significantly suppressed E2 and CYP-induced proliferation, EMT, migration, and invasion of Ishikawa cancer cells. Overall, the present study revealed that DIM has an antiestrogenic chemopreventive effect to withdraw the cancer-enhancing effect of E2 and CYP, while CYP has the capacity to enhance the metastatic potential of estrogen-responsive endometrial cancer.</P>

Potential Association Between Frequent Nonsynonymous Variant of <i>NPPA</i> and Cardioembolic Stroke

Kim, Jeong-Hyun,Jang, Bo-Hyung,Go, Ho Yeon,Park, Sunju,Shin, Yong-Cheol,Kim, Sung-Hoon,Ko, Seong-Gyu Mary Ann Liebert 2012 DNA and cell biology Vol.31 No.6

Copper indium selenide water splitting photoanodes with artificially designed heterophasic blended structure and their high photoelectrochemical performances

Kim, Joo Sung,Baek, Seung Ki,Kim, Young Been,Do, Hyun Woo,Kwon, Yong Hun,Cho, Sung Woon,Yun, Young Dae,Yoon, Jae Hong,Lee, Han-Bo-Ram,Kim, Sang-Woo,Cho, Hyung Koun Elsevier 2018 Nano energy Vol.46 No.-

<P><B>Abstract</B></P> <P>Conventional <I>p-</I>CuInSe<SUB>2</SUB> absorbers for solar cells have been homogenously synthesized using multi-step process despite a narrow crystal phase region in the phase diagram and the existence of various secondary phases. In contrast, here we propose artificially-designed heterophasic blended copper indium selenide compounds for water splitting photoanodes using a simple one-step annealing synthetic process where the electrodeposited metal precursors were directly annealed with Se vapor injection and without additional intermediate steps. The resultant product is revealed to possess a novel “<I>phase-blended structure</I>” comprising two phases of <I>p-</I>type CuInSe<SUB>2</SUB> and <I>n-</I>type CuIn<SUB>3</SUB>Se<SUB>5</SUB> crystals. The CuInSe<SUB>2</SUB> nanoparticles with a higher Cu fraction are three-dimensionally (3D) embedded in the <I>n-</I>type CuIn<SUB>3</SUB>Se<SUB>5</SUB> matrix, which has been verified by various analysis methods such as X-ray diffraction, transmission electron microscopy, and capacitance-voltage curve. The average diameter of the CuInSe<SUB>2</SUB> nanoparticles is 66.8nm and the interval between the nanoparticles in the CuIn<SUB>3</SUB>Se<SUB>5</SUB> matrix is 67.6nm. Consequently, the phase-blended structure photoabsorber exhibits a remarkably enhanced anodic photocurrent of 12.7mA/cm<SUP>2</SUP> at 1.23V versus the reversible hydrogen electrode. The considerably enhanced photocurrent gain of the phase-blended structure photoanode is attributed to the excellent charge separation facilitated by the built-in potential generated from the 3D <I>p-n</I> junction.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Inorganic heterophasic blended structure was synthesized via one-step annealing process of electrodeposited Cu/In bilayers. </LI> <LI> We designed inorganic heterophasic blended structure consisting of <I>p</I>-type CuInSe<SUB>2</SUB> nanoparticles and <I>n</I>-type CuIn<SUB>3</SUB>Se<SUB>5</SUB> matrix. </LI> <LI> The nanoscale CuInSe<SUB>2</SUB> nanoparticles with a higher Cu fraction were three-dimensionally embedded in <I>n</I>-type CuIn<SUB>3</SUB>Se<SUB>5</SUB> matrix. </LI> <LI> Phase-blended structure resulted in sufficient depletion and efficiently separated photo-generated electron-hole pairs. </LI> <LI> Phase-blended structure CIS showed remarkably enhanced photocurrent and good stability with an ultra-thin Al<SUB>2</SUB>O<SUB>3</SUB> layer. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>We report an inorganic heterophasic blended structure with remarkably enhanced photocurrent performance via built-in potential between <I>p</I>-type CuInSe<SUB>2</SUB> nanoparticles and <I>n</I>-type CuIn<SUB>3</SUB>Se<SUB>5</SUB> matrix.</P> <P>[DISPLAY OMISSION]</P>

Ovalbumin Induced Asthma in VDUP1 Knockout Mouse Model

Bo Ram Lee,Jeong Ha Lee,Du Min Go,Jae Hoon Choi,Dae Yong Kim 대한수의학회 2012 대한수의학회 학술대회발표집 Vol.2012 No.-

A case of pregnant women with psoriasis exposed to acitretin: teratogenic risk of acitretin treatment

( Bo Young Kim ),( Go Eun Kim ),( Tae Jun Park ),( Ji Hyun Park ),( Il-hwan Kim ) 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.1

Acitretin is a synthetic retinoid specifically used in severe psoriasis and disorders of keratinization. Teratogenicity is the most important safety issue of acitretin. Strict pregnancy prevention is required in women of childbearing potential who use acitretin. We report a 30-year-old female with pustular psoriasis who had been taking acitretin at 20mg/day before contraception and during pregnancy until 7 completed weeks after the last menstrual period. We normally avoid prescribing acitretin to women of childbearing potential, but she wanted to receive acitretin because of its effectiveness. She has two induced abortion history. We explained to the patient about teratogenicity of actiretin, and recommended contraception. Once her pregnancy was confirmed, we stopped using acitretin, and explained the possibility of teratogenicity. Also, the obstetrics service was consulted. Acitretin is one of the drug had Pregnancy prevention plan(PPP) in the European union. Pregnancy test and contraception should be performed before, during, and after the treatment for 36 months. However, there is no legal risk minimization measure such as PPP in Korea. Therefore, additional supports should be provided for the special policies for teratogenic drug. Physicians and pharmacists must rigorously apply the acitretin PPP recommendations, and patients must be fully informed about acitretin’s risk of teratogenicity.