비타민 E 보충이 연령이 다른 여자의 항산화 상태 및 면역능력에 미치는 영향

김연중 이화여자대학교 대학원 1998 국내박사

비타민E 투여가 미숙아 용혈성 황달에 미치는 영향

김혜승 梨花女子大學校 1983 국내석사

비타민 C와 E 섭취가 대학레슬링 선수의 혈중지질과 항산화요소에 미치는 영향

김규태 국민대학교 스포츠산업대학원 2005 국내석사

본 연구는 운동을 지속적으로 수행하고 있는 대학 레슬링선수들에게 6주 동안 항산화제 비타민 C와 E 섭취기간과 운동 강도별 활성산소의 작용에 의한 항산화효소의 활성 및 혈중지질이 어떻게 변하는 가를 분석하여 운동 강도와 이를 완화하는 항산화제 적정 투여기간을 규명하기 위한 것이다. 연구의 대상자들은 대학레슬링 남자 12명을 대상으로 6주간 항산화제 비타민 C와 E를 섭취하여 처치기간 (섭취전, 섭취 3주후 ,섭취 6주후)및 운동 강도에서 항산화 효소 (CAT)와 혈중지질의 변화를 알아보고자 하였다. 본 실험은 사전 실험을 기준으로 결정된 VO_(2) max75%의 운동 강도로 탈진에 이르는 트레드밀 운동을 수행하였다. 혈액 채취는 안정시 운동 중, 운동직후 총3회 실시 하였다. 비타민섭취 집단은 비타민 E1000IU, 비타민C 1000mg을 6주 동안 아침, 저녁에 하루2캅셀씩 복합 섭취 하도록 하였다. 섭취집단과 통제집단의 안정시, 운동 중, 운동직후에 따라 항산화효소, 혈중지질의 집단간, 집단내 변화를 알아보기 위하여 TWO-way repetead measure ANOVA를 사용하여 아래와 같은 결론을 얻었다. 첫째, CAT 농토는 항산화 활성에 있어 VO_(2) max75%수준의 운동 강도와 섭취전 안정시와 운동 중, 운동직후의 유의한 변화를 나타내지 않았다. 또한 3주간, 6주간의 항산화 비타민 C와E섭취 후에도 집단내 집단간 유의한 차이가 없었다. 둘째, 총 콜레스데롤 TC은 비타민 C와 E 섭취기간에 그룹 간, 시기 간에 따라 통계적으로 유의한 차이가 나타났다. 1주와 3주를 비교했을 때 유의한 차이가 없었지만 1주와 6주에서 섭취기간에 유의한 차이가 나타났다. 셋째, 고밀도 지단백 콜레스테롤HDL-C은 비타민 C와 E 섭취기간 그룹 간, 시기 간에 따라 통계적 유의한 차이가 나타나지 않았다. 1주나 3주를 비교했을 때 유의한 차이가 없었지만 1주와 6주에서 섭취기간에 유의한 차이가 나타났다. 넷째, 저밀도 지단백 콜레스데롤 LDL-C, 중성지질 TG 섭취기간 그룹 간, 시기 간에 따라 통계적으로 유의한 차이가 나타나지 않았다. 1주와 6주에서 비교했을시 유의 한 차이가 나타나지 않았다. 이상의 결론들을 종합하면 VO_(2) max75% 고정부하에서 탈신 시까지 운동을 실시한 결과 산화 스트레스의 변화가 거의 없는 것으로 나타났으며 항산화 비타민 C 와 E 섭취기간에 따른 운동 강도와 항산화능력도 크게 변화가 없는 것으로 나타나 추후 장기간의 적정량을 섭취하는 방법에 대한 종단적 연구가 필요한 것으로 사료된다. This research was to examine the appropriate dosage period of antioxidants that alleviate exercise stress. Twelve university wrestling athletes were given vitamin C and E for 6 weeks. During this period (The change during the diagnose period before intake, 3 weeks after intake, 6 weeks after intake) the changes in antioxidant enzyme activity and blood lipid levels in different exercise intensity were investigated among athletes. The experiment was performed on the trademill until the exhaustion at the 75% VO_(2) max exercise-intensity obtained in the pre-treatment. Blood samples were taken 3 times: in the stable state(before the exercise), during the exercise state, and after the exercise state. The vitamin group; 1000mg of vitamin C and 1000IU of vitamin E, ware taken two capsules a day, once in the morning and once in the evening. A TWO-WAY repeated measure ANOVA was used to see the change in the antioxidant enzyme and the blood-Lipid level within and between the supplement-group and the control-group at the stable, during the exercise and after the exercise state. The results are as follows. First, the CAT activity did not show any related changes between the antioxidant activation 75% VO_(2) max exercise-intensity and the stable, during exercise and the after exercise state. Also after the third and sixth weeks period of the antioxidant supplement there were no significant differences within or between groups. Second, the total cholesterol(TC) showed considerable statistical difference between groups and in the consumption period of the vitamins C and E. There were no notable changes when the first and third week was compared. However, there were significant differences in TC between the first and sixth week intake period. Third, the high-density lipoprotein cholestrol(HDL-C) did not display any statistical differences between groups and in the consumption period of vitamins C and E. There were no notable changes when the first and third week was compared. However, there were significant differences between the first and sixth week intake period. Fourth, there was no significant differences between groups and in the consumption period of the low-density lipoprotein cholesterol(LDL) and the triglycerides(TG). There was no differences between the first and the sixth week. The conclusion is as follows. When exercised starting from the 75% VO_(2) max fixation to the exhaustion the antioxidant activity was not changed. There were not much changes in the exercise intensity, antioxidant activity, and blood lipid levels during the intake period of the antioxidant vitamin C and E. It is recommended to consume the subscribed amount of the antioxidant vitamins for a long period of time rather than a large amount in a short period.

식이내 비타민 E 수준과 단기간의 운동이 마우스 조직의 항산화효소 활성과 혈청 IL-2 농도에 미치는 영향

이소연 숙명여자대학교 일반대학원 1998 국내석사

사람을 포함한 고등동물의 필수 영양소인 비타민 E 는 불포화지방산의 과산화작용을 저해하여 과산화물로부터 생체를 보호하고 면역기능에 영향을 주어 암세포의 성장을 억제하며, 운동은 산화적 스트레스를 증가시키고 면역능력을 변화시켜 암발생에 대한 억제효과가 있음이 보고되고 있다. 따라서 본 연구에서는 식이내 비타민 E 수준과 단기간의 운동이 조직에서 생성되는 항산화효소 (superoxide dismutase, glutathione peroxidase, catalase)의 활성과 혈청 IL-2 농도에 미치는 영향을 알아보고자 ICR 계 수컷마우스 (4-6 주령, 48마리)를 식이 비타민 E 수준에 따라 저비타민 E 식이군 (40mg/kg diet vitamin E), 정상수준의 대조군 (80mg/kg diet vitamin E), 고비타민 E 식이군 (200mg/kg diet vitamin E)으로 분류하여 8주간 실험식이를 제공하고 실험종료 직전 3일간에 걸쳐 treadmill 운동 (18m/min, 60min/day, 5% grande)을 실시하여 실험기간 종료후 비타민 E 수준을 측정하고 식이내 비타민 E 수준과 단기간의 운동에 의한 효과를 비교하였다. 8주간의 실험식이후 혈청과 간의 비타민 E 농도는 식이내 비타민 E 수준이 증가함에 따라 유의적으로 증가하였으며 total SOD 활성은 신장에서 저비타민 E 식이와 고비타민 E 식이시 감소하였으나 심장에서는 고비타민 E 식이시 증가하였다. Cu, Zn-SOD 활성은 신장에서 고비타민 E 식이시 유의적으로 감소했지만 근육에서는 저비타민 E 식이시 증가하였으며 Mn-SOD 활성은 모든 조직에서 식이 비타민 E 수준에 따른 유의적인 차니가 없었다. GPX 활성은 폐에서는 저비타민 E 식이시, 심장에서는 고비타민 E 식이시 증가하였으며 catalase 활성은 심장에서 고비타민 E 식ㅇ시 증가하였다. 운동에 의해서는 폐에서 total SOD, Cu, Zn-SOD, Mn-SOD, GPX, catalase 활성이 모두 증가하였으나 간과 신장에서의 GPX 활성은 감소하였다. 또한 항산화효소 활성에 대한 식이 비타민 E 수준과 운동간의 상호작용은 모든 조직에서 나타나지 않았으며 혈청 IL-2 농도도 식이 비타민 E 수준과 운동에 의해 유의적인 차이가 없었다. 이상의 결과를 종합해 볼 때 식이 비타민 E 수준은 항산화효소 활성에 대한 조직 특이성이 있어 고비타민 E 식이시 신장에서는 감소하고 심장에서는 증가하는 것으로 나타났으며, 단기간의 운동에 의해서 특히 폐조직의 항산화효소 활성이 증가하는 것으로 나타났다. 이와 같은 결과는 비타민 E의 항산화효과와 함게 생체내에 운동에 의해 생성된 radical 을 제거해주는 방어기전이 있음을 규명하는데 도움이 될 것으로 사료된다. Vitamin E, essential for life in higher animals including human, is thought to protect the body by inhibiting polyunsaturated fatty acid peroxidation and modulate the immune functions. While, exercise increases the oxygen consumption in the body, thereby enhances the oxidative stress to various organs and alter the immune response. The effect of dietary vitamin E levels and acute exercise on tissue antioxidant enzyme activities (superoxide dismutase, glutathione peroxidase, catalase) and serum IL-2 concentration were studied in mouse. Forty-eight one-month-old ICR mouse were fed with one of three different diets : low vitamin E (40mg/kg diet vitamin E), control (80mg/kg diet vitamin E), high vitamin E (200mg/kg diet vitamin E) diet for 8 weeks. Mouse exercised (18 m/min, 60 min/day, 5% grade) on a motor-driven treadmill for the last three consecutive days. Mice were killed immediately after exercise, blood and tissue(liver, lung, kidney, heart, and muscle) were collected to investigate the antioxidant enzyme activities and serum IL-2 concentration. Dietary vitamin E supplementation increased serum and liver vitamin E concentration. The activity of total superoxide dismutase(SOD) was significantly decreased (p<0.05) in the kidney of low and high vitamin E group and increased (p<0.05) in the heart of high vitamin E group. The activity of Cu, Zn-SOD was significantly decreased (p<0.05) in the kidney of high vitamin E group and increased (p<0.05) in the muscle of low vitamin E group. However, no significant differences in Mn-SOD activity were observed in all mouse tissues. The activity of GPX was significantly increased (p < 0.05) in the lung and heart of high vitamin E group. the activity of catalase was significantly increased (p<0.05) in the heart of high vitamin E group. Acute exercise significantly increased (p<0.05) total SOD, Cu, Zn-SOD, Mn-SOD, GPX, catalase activities in lung, but decreased (p<0.05) GPX activity in liver and kidney. there was no significant differences in serum IL-2 concentrations among different diet and exercise groups. The result suggested that the change of mouse antioxidant enzyme activities by different diatary vitamin E levels were tissue specific. And acute exercise was found to increase antioxidant enzyme activities in lugn tissue.

사염화탄소로 유발된 흰쥐의 급성간손상에 대한 비타민 E의 효과

홍순호 建國大學校 農畜大學院 2000 국내석사

간세포는 다양한 원인에 의해 손상을 받으면 세포 상호작용에 의해 각종 사이토카인 및 산소 유리기 등이 생성되면서 세포외기질이 손상을 받게 되고, 이로 인해 세포외기질이 이상증식이 유발되면 간섬유증으로 진행하게 된다. 단기적인 사염화탄소 투여시 급성 간손상을 일으켜 일시적인 간조직의 섬유화를 유발시킬 수 있고, 산화성 스트레스가 간섬유화의 중요한 요인으로 생각되고 있지만, 그 기전에 대해서는 확실하게 밝혀진 것이 없다. 간성상세포와 변환성장인자(TGF-β_(1))가 간섬유화를 유발시키는 중요한 요인으로 생각되므로 본 연구에서는 사염화탄소에 의한 급성 간손상시 간성상세포 골격표지자인 desmin과 세포증식에 관여하는 PCNA 항체를 이용하여 간성상세포의 활성도와 증식능을 관찰하였다. 또한 변환성장인자의 발현을 시간대에 따라 관찰하고, 비타민 E의 투여에 따른 변화를 비교 검토하여 항상화제인 비타민 E의 효과에 대해 알아보고자 면역조직화학적 방법을 시행하였다. 본 연구에서 도출된 결과는 다음과 같다. 1. 사염화탄소 투여에 따른 간성상세포의 활성도와 증식능의 변화는 사염화탄소를 투여한 Ⅰ군에서 사염화탄소 투여전과 비교시 시간이 지남에 따라 desmin과 PCNA발현이 증가되므로 간성상세포는 교원질을 생성하여 간섬유화를 일으킬 수 있는 세포라는 것을 확인할 수 있었다. 2. 사염화탄소를 투여한 Ⅰ군에서 사염화탄소 투여전과 비교시 시간이 경과함에 따라 변환성장인자의 발현이 증가되었다. 변환성장인자는 간섬유화를 유도하는 사이토카인 중의 하나로 이것의 증가는 간성상세포를 자극하여 섬유화를 유발시키는 중요한 역할을 하는 인자라는 것을 알 수 있었다. 3. 비타민 E 투여에 따른 간성상세포의 활성도와 증식능의 변화는 비타민을 투여한 Ⅱ군과 비타민 E를 투여하지 않은 Ⅰ군에서 32시간대에 desmin과 PCNA의 발현이 증가되었으나, Ⅰ군에 비해 Ⅱ군에서 desmin 과 PCNA의 발현이 유의하게 감소되었다. 또한 비타민 E투여에 따른 변환성장인자의 변화는 두 군 모두 32시간대에 발현이 증가되었으나, Ⅰ군에 비해 Ⅱ군에서 변환성장인자의 발현이 유의하게 감소되었다. 이는 항산화제인 비타민 E가 간성상세포와 변환성장인자의 활성을 억제시키는 것을 확인하였다. 이상의 결과에서 사염화탄소에 의한 급성간송상시 간성상세포와 변환성장인자는 간섬유화로 진행하는데 중요한 역할을 하며, 산화성 스트레스에 의한 간손상시 항상화제인 비타민 E는 급성 간부전의 치료 및 만성 간손상에 의한 간섬유화의 예방에 효과적일 것으로 사료된다. After the liver is damaged from an insult, various cytokines and free oxygen radicals are released from that damage the extracellular matrix which in turn induces abnormal hyperplasia of the extracellular maxtrix leading to hepatic fibrosis. Short term administration of CCl_(4) induces acute hepatotoxicity and may cause transient hepatic fibrosis. Although oxygen stress is thought to be an important mediator of this process the exact mechanism is not yet known. Hepatic stellate cell and TGFβ_(1) are demonstrated to be important agents in the formation of hepatic fibrosis. Thus, this study aimed to determine the changes in the hepatic stellate cell after induction of acute hepatoxicity with CCl_(4). desmin, a skeletal marker of hepatic stellate cell, and PCNA antibody that is related to cellular growth were used to determine the activity and growth potentionial of hepatic stellate cell. TGF-β_(1) expression was observed in relation to time and vitamin E administration. The effect of vitamin E, an anti-oxygen agent, was determined by immunohisto chemistry. The results are as follows: 1. Desmin and PCNA expressions were increased after CCl_(4) treatment. This result demonstrated that stellate cell could induce hepatic fibrosis through the production of collagen. 2. TGF-β_(1) activity was increased with time after CCl_(4) administration. This result showed that TGF-β_(1) was an important factor in the development of hepatic fibrosis. 3. Although desmin, PCNA, and TGF-β_(1) expressions were all increased at 32 hours in both the vitamin E administered and control groups, the levels were all significantly lower in the vitamin E administered group. These data demonstrated that vitamin E inhibits stellate cell and TGF-β_(1) activity. Taken together, These results suggest that stellate cell and TGF-β_(1) are important mediators of hepatic fibrosis after acute hepatotoxicity from CCl_(4). Vitamin E may be effective for the treatment of acute hepatic damage from oxygen stress and prevention of hepatic fibrosis from chronic hepatic damage.

Vitamin E 濟가 全身持久力 向上에 미치는 影響 : 運動選手와 一般學生을 中心으로

김교성 漢陽大學校 大學院 1983 국내석사

비타민 C, E 섭취가 전신경락마사지로 인한 피로도 저감화에 미치는 영향

김영순 건국대학교 대학원 2016 국내박사

본 연구는 비타민 C, E 복합 섭취가 전신경락마사지 후 대사산물로 인해 올 수 있는 피로도와 통증의 변화, 혈액 내 피로물질 및 항산화 효소들의 농도변화에 미치는 영향을 알아보고자 하였으며 경기지역에 거주하는 30∼50대 여성을 대상으로 실험유형별로 대조군(a meridian massage treatment control group, Cont.) 8명, Vit. C군(a meridian massage treatment after intake vitamin C group, Vit. C) 8명, Vit. E군(a meridian massage treatment after intake vitamin E group, Vit. E) 8명, Vit. CE군(a meridian massage treatment after intake vitamin C and vitamin E group, Vit. CE) 8명으로 총 4그룹으로 나누어 진행하였다. 연구결과는 피로정도와 통증정도의 변화에서 Vit. CE 모두 8.7%의 낮은 증가 경향을 보였고, 젖산 농도는 Cont.군에서 30.8% 증가, Vit. C군은 33.9% 감소, Vit. E군은 30.1% 감소, Vit. CE군은 45.5% 감소하였다. 요산은 Cont.군에서 2.3% 증가, Vit. C군, Vit. E군, Vit. CE군은 각각 6.6%, 8.9%, 9.5%로 유의하게 감소하였다. 코티졸 농도는 Cont.에서 약 32% 증가하여 산화스트레스가 유발되었고, 항산화제 섭취군인 Vit. C, Vit. E, Vit. CE에서 각각 5%, 16.2%, 23.4% 순으로 감소하였으며, 카테콜아민 농도는 비타민 복합 섭취군인 Vit. CE군에서 13.9% 감소한 것으로 나타나 전신경락마사지 시행 시 비타민 복합 섭취는 카테콜아민 반응을 효과적으로 둔화시키는 것으로 나타났다. SOD는 Cont.군에서 56% 증가, Vit. C군은 40.7%, Vit. E군은 46.6% 증가, Vit. CE군은 30.2% 증가하였고, GPx는 Cont.군에서 4.1% 감소, Vit. C군은 17.1% 증가, Vit. E군은 2.9% 감소, Vit. CE군은 17.4% 증가, BAP 는 Cont.군에서 0.4% 증가, Vit. C군, Vit. E군, Vit. CE군은 7.3%, 3.8%, 2.1% 증가하였으며, d-ROMs 변화는 Cont.군에서 1.5% 감소, Vit. C군, Vit. E군, Vit. CE군은 3.3%, 3.9%, 5.4% 감소하였다. 따라서 운동 등으로 굳어진 부위와 아픈 부위의 통증을 완화시키기 위한 대체제로 비타민 C와 E의 복합 섭취 및 경락마사지의 활용이 촉구된다. This study evaluated the impact of the simultaneous intake, vitamin C and E, on the concentration changes of fatigue and pain levels and antioxidant enzyme in blood, due to the metabolites after a body meridian. It targeted on women in their 30’ to 50’s living in Gyeonggi Province. By experimental types four target groups follow : a meridian massage treatment control group, Cont. as 8 people, Vit. C as 8 (a meridam massage treatment after intake vitamin C group), Vit. E as 8 (a meridian massage treatment after intake vitamin E group), Vit. CE as 8 (a meridian massage treatment after intake vitamin C and vitamin E group). The Result of the changes in fatigue and pain levels, was increased a little 8.7% in all including Vit. CE. The latic acid concentration was increased 30.8% in Cont., but decreased 33.9% in Vit. C, 30.1% in Vit. E, 45.5% in Vit. CE. The uric acid was increased 2.3% in Cont. but decreased attentively 6.6%, 8.9%, 9.5% in each Vit. C, Vit. E, Vit. CE. SOD (superoxide dismustase) was increased 56% in Cont., 40.7% in Vit. C, 46.6% in Vit. E, 30.2% in Vit. CE. GPx (glutathione peroxidase) was decreased 4.1% in Cont., 2.9% in Vit. E but increased 17.1% in Vit. C, 17.4% in Vit. CE. BAP (biological antioxidant potential) was increased 0.4% in Cont., 7.3%, 3.8%, 2.1% in each Vit. C, Vit. E, Vit. CE. d-ROMs (diacron reactive oxygen metabolites) was decreased 1.5% in Cont., 3.3%, 3.9%, 5.4% in each Vit. C, Vit. E, Vit. CE. From this, you can see each works in advanced way, like lactic and uric acid were originally increased, but with vitamin intake, they were decreased. Therefore, vitamin C and E intake before meridian massage treatment is highly recommended to alleviate pain in sore or hardened areas after exercise.

비타민 C 및 E 급여가 거세우의 도체특성 및 육질에 미치는 영향

추교문 慶尙大學校 大學院 2004 국내석사

This experiment was carried out to investigate the effects of dietary vitamin C and E on carcass characteristics and meat quality of Hanwoo steers. Thirty seven Hanwoo steers in the last fattening period were randomly assigned to one of four treatments. The four treatments were control(vitamin E 20IU/kg feed), vitamin C(vitamin C 0.1%/kg feed and vitamin E 20IU/kg feed), vitamin E( vitamin E 220IU/kg feed) and 0.1% vitamin plus 200IU vitamin E(vitamin C 0.1%/kg feed and vitamin E 220IU/kg feed). Hanwoo steers were fed experimental diets for 3 months of the last finishing period. Animals fed the diet containing 0.1% vitamin C plus 200IU vitamin E had higher(P<0.05) yield grade than those fed the other diets but quality grade was not different between animals fed the control diet and vitamin-supplemented diets. Proximate analysis in longissimus dorsi was not different between animals fed the control diet and vitamin-supplemented diets. Vitamin C concentration in longissimus dorsi was higher(P<0.05) in the animals fed the vitamin C-supplemented diet and the vitamin C plus E diet than in those fed the control diet and the vitamin E diet. Vitamin E concentration in longissimus dorsi was higher(P<0.05) in the animals fed the diet supplemented with vitamin E and the diet supplemented with vitamin C plus of vitamin E than in those fed the control diet and the vitamin C diet. Animals fed the diet supplemented with vitamin C was higher(P<0.05) in myristic acid, palmitic acid, palmitoleic acid and oleic acid in the longissimus dorsi than those fed the other diets but linoleic acid in longissimus dorsi was higher(P<0.05) in the animals fed the vitamin E-supplemented diet than those fed other diets. However, saturated fatty acid and unsaturated fatty acid were not different between animals fed the control diet and vitamin-supplemented diets. pH value in longissimus dorsi was increased as the storage period elapsed and was higher in the animals fed the vitamin C plus E diet than in those fed the other diets during the whole storage period. TBARS in longissimus dorsi was lower(P<0.05) in the animals fed the vitamin-supplemented diets than in those fed the control diet. Especially it was lowest(P<0.05) in the animals fed the diet supplemented with vitamin C and vitamin E. Therefore, it is supposed that lipid oxidation in longissimus dorsi can be retarded when vitamin C and vitamin E are supplemented with diet. Hue angle in longissimus dorsi was increased as the storage period prolonged. Especially it was lower(P<0.05) in the animals fed the vitamin-supplemented diets than in those fed the control diet. Metmyoglobin concentration in longissimus dorsi was increased as the storage period lengthen. Especially it was lower(P<0.05) in the animals fed the diets supplemented with vitamins C or vitamin E than in those fed the diet supplemented with no vitamins. Oxymyoglobin concentration in longissimus dorsi was higher(P<0.05) in the animals fed the diets supplemented with vitamin C or vitamin E than in those fed the control diet. Therefore, it is considered that vitamin C and E can stabilize beef color in longissimus dorsi when supplemented or with diet, respectively. Water holding capacity in longissimus dorsi was decreased as the storage period elapsed and was not different between animals fed the control diet and those fed the vitamin-supplemented diets until 7 days of storage. Cooking loss in longissimus dorsi was higher(P<0.05) in the animals fed the diets supplemented with vitamin E or vitamin C plus vitamin E than in those fed the control diet or vitamin C-supplemented diet. Drip loss and purge loss in longissimus dorsi were increased as the storage period elapsed. According to these results, it may be concluded that feeding the diets containing 0.1% vitamin C or 200IU vitamin E or the diet mixed these vitamins in 0.1% and 200IU to Hanwoo steers can retard lipid oxidation of longissimus dorsi and stabilize beef color.

비타민E 급여가 한우육질에 미치는 영향

이동길 경북대학교 농업개발대학원 2006 국내석사

This study was carried out to investigate the effect of vitamin E on Hanwoo meat quality. Hanwoo steers were fed vitamin E of 2mg per day for 15, 30, and 45 days, and their meats were analyzed about meat color, shear force, lipid oxidation, pH, water holding capacity, juiciness, tenderness, and flavor. The results obtained were as follows. Vitamin E fed for over 30 days improved the meat color, reduced the shear force, delayed the lipid oxidation, and increased the water retention capacity, but didn’t affect pH change. However, vitamin E increased meat quality by improving the organoleptic traits like juiciness, tenderness, and flavor of the meat In conclusion, vitamin E might improve the quality of Hanwoo meat, if Hanwoo steers were fed with vitamin E of 2mg a day for at least 30 days.

사염화탄소로 유발된 흰쥐 간성상세포의 손상에 대한 비타민 E의 예방효과

홍성일 建國大學校 農畜大學院 2001 국내석사

This study has measured ALT (alanine aminotranferase), AST (aspartate aminotranferase), LDH (lactate dehydrogenase) and total bilirubin as the hepatic index as time passed after inducing hepatic injury by giving carbon tetrachloride (CC1_(4)) to a male rat. The α-smooth mucle actin immunohistochemistry method was employed to observe the degree of activation of hepatic stellate cell (HSC) and the changes in the number of HSC as time passed were recorded. Vitamin E that is well known as an antioxidant was investigated to prove that it may prevent the hepatic injury caused by oxygen stress that were induced by CC1_(4). Results from this study were summarized as follows; 1. After administration of CC1_(4) changes in weights of liver and body of rat's according to each 100 g of weight of a rat were examined. Body weights were decreased and but liver weights were increased in comparison with the control group. Administration of vitamin E showed significant increase in the weight (P<0.05). 2. ALT, AST, LDH and total bilirubin in serum were examined and the group treated with administration of vitamin E showed the significant decrease (P<0.05) in comparison with the group treated with administration of CC1_(4). Interestingly, both groups showed the highest level's of them after 32 hours (P<0.01). 3. The result in immunohistochemistry staining of α-SMA demonstrated that in comparison with the group treated with only CC1_(4), the group treated with administration of vitamin E showed noticeable decrease (P<0.05). 4. The significant relationship between serum ALT and α-SMA expressions speculated to prove that as hepatic injury got worse, the number of HSC simultaneously increased (R=0.692). Taken together, the degree of hepatic injury after giving CC1_(4) to rats was the worst after 32 hours and the degree of expressions of α-SMA were increased or decreased according to the degree of hepatic injury. Furthermore, The administration of the antioxidant vitamin E were reduced the degree of hepatic injury and inhibited the activation of HSC. It is, therefore, concluded that vitamin E may have a beneficial role to prevent hepatic injury caused by oxygen stress.