인체 체액내 황색포도상구균의 증식과 철 획득 기전

신성희,이강길,이명희,양남웅,임용,김진호 대한감염학회 2000 감염 Vol.32 No.5

Drosophila melanogaster 韓國 自然集團에 있어서 P-M system에 依據한 系統型 轉換

成耆昌,崔喜慶,金溶聲 성균관대학교 기초과학연구소 1989 論文集 Vol.40 No.1

Isofemale lines of Drosophila melanogaster were established from Daejon in Korea and were examined in order to investigate the changes of cytotype or strain type by P-M system through 1∼15 generations. The results are as follows: 1. Using π_2 (strong P) strains and bw;st (true M) strains as standard strains, the mean sterility frequencies from Cross A were 20.8%∼34.6% for each generations and the mean sterility frequencies from Cross B were 0.0%∼0.3%. 2. Frequency of strain with P cytotype for each generation was analyzed to be 44.8%(G₁), 59.2%(G₂), 43.7%(G₄), 36.7%(G_7), 29.2%(G_10) and 36.7%(G_15). Therefore it was tend to be low as increased with generation number, but the differencies in percentage of GD sterility between each generation are non-significant statistically. 3. Among the isofemale lines, type conversions were observed. Q→M', M'→Q and M'→M changes have occured with frequency of 18.4%, 8.2% and 4.1%, respectively. The remaining lines (69.3%) were unchanged through several generations. 4. The results suggest that Drosophila melanogaster are appeared temporally as a certain strain type in natural population, but the type conversions were represented by interactions between P elements in each genome and cytoplasm for each generation.

한국재래산양 β-lactoglobulin 유전자 5'flanking 영역의 염기서열 분석

류승희,한성욱,서길웅,상병찬 충남대학교 농업과학연구소 2001 농업과학연구 Vol.28 No.2

본 연구는 한국 재래산양의 β-lactoglobulin(β- LG) 유전자 발현조절부위의 특성을 구명하기 위하여 PCR기법으로 specific primer를 이용하여 β-LG 발현조절부위를 증폭한 후 염기서열을 분석하여 Sheep종의 β-LG유전자 발현조절부위의 염기서열상의 차이를 분석하였다. 한국 재래산양의 genomic DNA로부터 PCR기법을 이용하여 β-LG 유전자 발현조절 부위를 증폭한 결과 Sheep종에서 보고된 바와 같이 1,077bp의 단편크기로 증폭되었음을 확인 할 수 있었다, Sheep종에서 보고된 β-LG 발현조절 부위의 염기서열과 한국재래산양에서 분석된 염기서열간의 차이는 총 897개의 염기중 46개의 nucleotide에서 차이를 나타내어 94.9%의 상동성을 보였다. 특히 한국재래산양과 Sheep종간 염기서열상의 차이는 Sheep종의 T염기가 재래산양의 C염기로 치환되었거나 반대로 Sheep종의 C염기가 재래산양에서 T염기로 치환되어 있음을 확인할 수 있었다. 이상의 결과에서 β-LG유전자 발현조절부위의 염기서열은 재래산양과 Sheep 종간에 높은 상동성을 보였으나 이들 종간의 발현조절부위의 염기서열상의 차이에 대한 유전자 발현조절 관계와 전사요소는 앞으로 연구가 더 진행되어야 할 것으로 사료된다. This study was analyzed by PCR technique with specific primer in order to investigate the characteristics of β-lactoglobulin (β-LG) gene 5'flanking region in Korean native goat. This work confirmed amplified product of 1,077 bp fragments obtained from the amplification of β-LG promoter from genomic DNA using PCR in Korean native goat. The nucleotide sequence of β-LG gene 5'flanking region in Korean native goat as compared with that of sheep β-LG were different at 46 base of 897 nucleotides, and showed high homology as about 94.9% each breed. Especially we confirmed that the difference of nucleotide sequences between Korean native goat and sheep were consisted of T→C substitution and C→T substitution reversely. As a consequences, the sequences of β-LG gene 5'flanking region showed a high homology between Korean native goat and sheep. Furthermore we should be studied that relationships between the control of gene expression and nucleotide sequences of transcription factor in Korean native goat.

전영택 소설의 죽음 연구

김용성,임희종 全北大學校 敎育大學院 1998 敎育論叢 Vol.18 No.-

Jeon Yeongtaeg, with a penname Nuˇlbom which means spring-ever, endeavored to write about Death, criticizing against the popular romantic novels of Lee Kwangsoo's style. My study over his 17 short novels of the theme of Death will give light to the of Death in his literary works. It seemed to me that his love for people which rules his works all the time was not so much influenced by the Christian faith but as his very nature. He expressed archetype of Death and revival through consideration of Christianism. The aspects of Death in his novels are Death locked in feudalistic values, Death of deliverance in restriction, Death of redemption in the favor of Salvation, Death of martyrdom against persecution and Death for the sake of rebirth.

Rockfill 댐의 Hydraulic Fracturing에 關한 硏究

임희대,우원재,우상태 忠南大學校 産業技術硏究所 1995 산업기술연구논문집 Vol.10 No.2

The term hydraulic fracturing has been used in the geotechnical engineering literature to describe situations in earth embankment dams where the reservoir water pressure initiates and propagates a cracks. The term has also been used in situations where an existing crack is further widened by the reservoir water pressure. The mechanisms of hydraulic fracturing in the core of rockfill dams that have been proposed to date are quite qualitative and have been postulated in the course of investigations to find the causes of leakage or failures as a results of internal erosion. In this study, the various mechanisms of hydraulic fracturing are outlined and the finite element analyses of 5 representative rockfill dams in Korea were conducted to examine the assumption that hydraulic fracturing occurs in embankment cores when the reservoir water pressure exceeds the total normal stresses. This numerical analyses use the hyperbolic model and its parameters from the conventional triaxial tests. As the results of the analyses, the total normal stresses by FEM exceed the water pressures in most cases which is contray to the fact that the water pressures exceed the total normal stresses by the pressure meters in the case of Soyanggang dam.

비선형 시스템 제어를 위한 퍼지 PID 제어기의 설계 및 해석

이철희,김성호 강원대학교 산업기술연구소 2000 産業技術硏究 Vol.20 No.B

Although Fuzzy Logic Controller(FLC) adopted three terms as input gives better performance, FLC is in general composed of two-term control because of the difficulty in the construction of fuzzy rule base. In this paper, a three-term FLC which is similar to PID control but acts as a nonlinear controller is proposed. To reduce the complexity of the rule base design and to increase efficiency. a simplified fuzzy PID control is induced from a hybrid velocity/position type PID algorithm by sharing a common rule base for both fuzzy PI and fuzzy PD parts. It is simple in structure, easy in implementation, and fast in calculation. The phase plane technique is applied to obtain the rule base for fuzzy two-term control and the resultant rule base is Macvicar-Whelan type. And the membership function is a Gaussian function. The frequency response information is used in tuning of the membership functions. Also a tuning strategy for the scaling factors is proposed based on the relationship between PID gain and the scaling factors. Simulation results show better performance and the effectiveness of the proposed method.

m-Methylphenyl N-methylcarbamate 유도체들의 살충활성 메카니즘

박승희,남상길,성낙도 충남대학교 농업과학연구소 1995 농업과학연구 Vol.22 No.2

A Series of meta and para-substituted phenyl N-methylcarbamate derivatives were synthesized and influence of substituents(X) on the molecular orbital(MO) quantities of carbonyl group, carbamylation reaction center, and insecticidal activities(pI_50) were discussed quantitatively. From these findings, the most stable streo conformer(Z) shows that the planer phenyl group occupies vertical(θ=90˚) position on the plane of the N-methylcarbamyl group. The carbamylation mechanism was proposed that the carbamylation process of acetylcholinesterase(ACh.E) by m-methyl substituted phenyl N-methylcarbamate derivatives proceeds via hyperconjugation of m-methyl group and carbonyl oxygene protonation, theromodynamically control reaction with acidic site of ACh.E.

주택에 있어서 가전기기 및 Home Automation 의 이용실태에 관한 조사연구

조성희,김계동 부산대학교 가정대학 1993 家政大學硏究報告 Vol.19 No.-

In housing design, the possession of many kinds of electric home appliances EHA and the supply of home automation(HA) are considered as new additional design criteria to develop the shape and area of kitchen and working space. This study attempts to findout the character of the possessins and usage of EHA and the HA. For this purpose, the field survey with the questionnaire and observation has been carried out based on 77 households by housing types (apartment and detached house). Findings are as follows : (1) The households have many kinds of EHA and the possession rate of them is very high. Also, the user preference for larger size of EHA such as refrigerater and washing machine is one of the usage characters of EHA. But, these characters of the possession and usage of EHA have not been considered on the design of kitchen and working area, so the misfits have many problems which are the narrow working space, the lack of storage space, the dead space and the inefficient working circulation in those areas. (2) The households require and recognize the HA as the important home equipment for the home security, the home control and the home management. But the practical usage rate is very low because of the supply system that the HA has been furnished by housing developer not user. So, the user education to give the information and instruction about HA is needed.

컴퓨터 그래픽을 이용한 실내 색채의 시각적 효과에 관한 연구 : 거실을 대상으로

조성희,윤소연 부산대학교 가정대학 1995 家政大學硏究報告 Vol.21 No.-

This study attemps to develop a tool to evaluate the color images and to analyze the visual effects of interior colors. For this purpose, the color models for living room using simulation method of computer graphics, as an evaluation tool, were developed and the experiment using them has been carried out to investigate the visual effects of interior colors based on 63 subject ratings. The color combination types for color models are composed according to principles of the lightness (light tone/dark tone) and the similarity or variety of 'tone' and 'hue' (unity, soft, varity, hard). And all of these types are made by three dominent color groups (10R, 5Y, 7.5B). The major findings are as follows; 1) The color models were defined as an effective evaluation tool for the visual effects of interior color. 2) As a result of factor analysis, four dimensions came out: 'pleasure', 'warmness', 'potency' and 'arousal'. 3) The light tone color combination types showed significant difference in 'pleasure' and 'warmness' from the dark tone groups. The light tone groups are grasped as more opened, more pleasant, more feminine, softer. On the contrary, The dark tone groups are comparatively more packed, masculine, rigid. Regarding overall circumstances, the light tone groups demonstrate bigger difference dependent on dominant colors than the dark tone groups, therefore, the property of the light tone influences on color image than any other factors. 4) Among the four combination types, unity(identity of hues-analogy of tones) revealed as the most unified images and B group was the highest grade. Moreover, it is affected a lot bydominant hue. Soft (variety of hues-analogy of tones) type shows eminently feminine, soft, and warm images. Variety (variety of hues-contrast of tones) type exhibit unnatural, artificial mood and is less affected by the dominant hues. Hard (identity of hues-contrast of tones) type is grasped as well arranged, exhibits simple and unified images. 5) General trend of the four types exhibits that harmony principle of color governs visual effect more than that of tones, for unity/Hard and Soft/Variety has similar trends.

Dextranase 함유 구강 세정액의 치태 제거 및 치은염 예방 효과에 관한 임상적 연구

송우성,손성희,정현주 전남대학교 치의학연구소 2001 구강과학 Vol.13 No.3

A novel glucanhydrolase (DXAMase) from a mutant of Lipomyces starkeyi (KSM 22) has been shown effective in hydrolysis of mutan, reduction of mutan formation by Streptococcus mutans and removal pre-formed sucrose-dependent adherent microbial film and DXAMase has been strongly bound to hydroxyapatitie. These in vitro properties of Lipomyces starkeyi DXAMase are desirable for its application as a dental plaque control agent. This study was performed to determine the adjunctive oral hygiene benefits and safety of dextranase (Lipomyces starkeyi KSM 22 DXAMase)-containing mouthwash when used alongside normal toothbrushing. This 6-month clinical trial was placebo-controlled double-blind design evaluating 1U/㎖ dextranase mouthwash and 0.12% chlorhexidine mouthwash. A total 39 systemically healthy subjects, who had moderate levels of plaque and gingivitis were included. At baseline, 1, 3 and 6 months, subjects were scored for plaque accumulation(Turesky modification of Quigley-Hein's plaque index), gingivitis status (Lo¨e and Silness gingival index), and tooth stain(Area and severity index system by Lang et al). Additionally, oral mucosal examinations were performed and subjects questioned for adverse symptoms. Two weeks after pre-experiment examinations and a professional prophylaxis, the subjects were provided with allocated mousewash and instructed to use 20-ml volumes for 30s twice daily after toothbrushing. All the groups showed significant increase in plaque accumulation since 1 month of experiment. During 6 month's period, the Dextranase mouthwash group showed the least increase in plaque accumulation, compared to the Chlorhexidine mouthwash and placebo groups. As for gingival inflammation, all the groups showed significant increase during 6 months of experiment. The Experimental group (Dextranase mouthwash) also showed the least increase in gingival index score, compared to the Positive control (Chlorhexidine mouthwash) as well as the Negative control (placebo) groups. Whereas the tooth stain was increased significantly in the Positive control group, compared to the baseline score and the Negative control group since 3 months of mouthrinsing. It was significantly increased after 6 months in the Experimental group, still less severe than the Positive control group. As for the oral side effect, the Experimental group showed less tongue accumulation, bad taste, compared to the Positive control group. From these results, mouthrinsing with Lipomyces starkeyi KSM 22 dextranase provided adjunctive benefits to toothbrushing, comparable to 0.12% chlorhexidine mouthwash in inhibition of plaque accumulation and gingival inflammation and local side effects were if anything less frequent and less intense than chlorhexidine, with long-term use of the mouthwash. All data had provided positive evidence for Lipomyces starkeyi KSM 22 dextranase as an antiplaque agent and suggested that further development of dextranase formulations for plaque control are warranted.