항원제시세포로서 CD40-활성 B 세포와 수지상세포의 기능 비교

NGUYEN HOANG TUYET MINH 전남대학교 대학원 2008 국내석사

종양항원에 특이적인 세포독성 T 림프구를 이용한 종양의 세포면역 치료는 종양에 대해서 특이 반응을 보이면서 부작용이 매우 적은 치료법으로 주목을 받고 있다. 이와 같이 종양-특이 T 세포 면역 반응을 유발시키기 위해서는 T 세포에 효과적으로 항원을 제시할 수 있는 항원제시세포의 매개가 필수적이다. 가장 강력한 항원제시세포로 알려진 수지상세포를 임상에 적용하기 위해서는 수회에 걸쳐서 다량의 수지상세포를 투여하여야 하는데, 시험관 내에서 수지상세포를 증폭할 수 없을 뿐만 아니라 대량 생산하는데 고비용이 소요되어서 임상적용을 하는데 어려운 문제점이 있다. 본 연구자는 이러한 수지상세포의 단점을 보완하여 종양에 대한 세포면역치료의 효과를 증대시킬 수 있는 대체 항원제시세포로서 CD40-활성 B 세포의 이용 가능성을 알아보기 위해서 CD40-활성 B 세포와 수지상세포의 항원제시기능을 비교 분석하였다. 정상인의 말초혈액에서 단핵구를 분리한 후 다시 림프구와 단구를 분리하였다. 분리된 단구는 GM-CSF와 IL-4를 첨가한 배양액에서 미성숙 수지상세포로 분화시켰으며, 배양 6일째에 IL-1β, TNFα, IL-6 및 PGE2를 첨가하여 표준 성숙 수지상세포로 분화시켰다. 림프구는 IL-4를 첨가한 배양액에서 CD40 리간드를 핵산전달감염시킨 J558 세포주와 공조배양하여 14일동안 B 세포를 활성화시켰다. 유세포분석기를 이용한 면역표현형 분석에서 CD40-활성 B세포와 수지상세포에서 항원제시세포의 기능과 연관된 costimulatory factor (CD80, CD86), 부착분자(CD54) 및 MHC-II 분자의 발현 정도가 두 세포에서 유사하게 높게 발현되었다. CCR7은 CD40-활성 B 세포에 비해 수지상세포에서 현저히 높게 발현되었지만, CD40-활성 B 세포의 표면에서도 이 분자의 발현이 증가되었다. CD40-활성화 B세포의 자가 T 림프구와 동종 T 림프구에 대한 자극 능력은 수지상세포에 비해서 다소 낮았다. 종양 특이 세포독성 T 림프구는 자가 CD3+ T 세포를 ARH77 세포주 융해질이 부가된 CD40-활성 B 세포나 수지상세포로 1주일 간격으로 3차례 반복 자극하여 제조되었으며, 자가 종양에 대한 세포독성 T 림프구에서 분비된 IFF-γ 분비 세포의 수는 ELSPOT 분석을 통해 측정하였다. 수지상세포로 자극되어 제조된 종양 특이 세포독성 T 림프구는 CD40-활성 B세포로 자극되어 제조된 것 보다 IFN- 분비능이 더 높게 나타났다. 그러나 CD40-활성 B 세포는 시험관내에서 대량 증폭이 가능함으로 종양항원-특이 세포독성 T 세포를 유도할 때 종양항원을 부가시킨 CD40-활성 B세포의 수를 두배 증가시켜 배양할 경우 발현된 IFN-γ 분비 세포의 수가 현저히 증가되어 수지상세포와 유사한 효과를 나타내었으며, 세포종양에 대한 살상효과는 오히려 약간 더 높게 나타났다. 결론적으로 본 연구는 종양 특이적인 세포면역치료에 있어서 CD40-활성 B세포가 향후 수지상세포를 대체할 수 있는 차세대 항원제시세포로써 매우 유용한 역할을 할 수 있음을 제시하였다. The use of cellular vaccines reliably generates tumor antigen-specific T cells both in animal models and in human. Highly efficient antigen presentation by professional antigen presenting cells (APCs) is a requisite for the development of T cell mediated immunity in vitro and in vivo. The potential need for continuous application of large number of dendritic cells (DCs) which are though as the most powerful APC might be a principle obstacle for clinical use of DC vaccines. In this study, we evaluated and compared the functions of antigen presentation of CD40-activated B (CD40-B) cells with those of DCs to confirm whether CD40-B cells could be an efficient alternative source of APCs which can compensate the limitation of DCs for cancer immunotherapy. Lymphocytes and monocytes were isolated from peripheral blood mononuclear cells (PBMCs) of normal donors using Percoll gradient centrifugation. To generate CD40-B cells, lymphocytes were co-cultured with CD40L-transfected J558 cell line in the presence of IL-4. Monocyte-derived DCs were differentiated from the same donor with conventional cytokine cocktail of IL-1, TNFα, IL-6 and PGE2. Both of CD40-B cells and DCs consistently expressed a high level of cell surface molecules related to APC function, such as CD80, CD86, CD54 and HLA-DR. Although the level of expression of CCR7 on mature DCs was much higher than that on CD40-B cells, this molecule was clearly up-regulated on CD40-B cells. Although DCs had superior capacity of allogeneic T cell stimulation compare to CD40-B cells, the level of cytotoxicity of cytotoxic T lymphocytes (CTLs) induced by CD40-activated B cells pulsed with ARH77 lysates against ARH77 was higher than that of CTLs induced by DCs pulsed with ARH77 lysates. CTLs stimulated by mature DCs pulsed with ARH77 lysates showed a larger number of IFN- secreting cells than those stimulated by CD40-B cells pulsed with ARH77 lysates in a culture ratio of 5:1. However, CTLs stimulated by CD40-B cells pulsed with ARH77 lysates at the 1:2.5 culture ratio demonstrated the increased number of IFN γ-secreting cells against ARH77 as much as CTLs stimulated by tumor antigen pulsed DCs at 5:1 culture ratio displayed. Because CD40-B cells can be easily expanded in vitro, these results suggest that CD40-B cells are an excellent alternative source of professional APCs for antigen-specific immunotherapy for cancer.

Thermal control strategies for increasing lifetime for three-phase voltage source converters

Nguyen, Minh Hoang 중앙대학교 대학원 2023 국내박사

Wide-Tuning Range Ring type VCOs Using 0.13-μm CMOS Process

Hoang, Nguyen Minh Kangwon National University 2010 국내석사

Towards A Fault-Tolerant Routing Protocol for Mobile Ad hoc Network

Hoang Nguyen,  Minh Duc 명지대학교 대학원 2023 국내석사

MANET(Mobile Ad Hoc Network)은 기존 네트워크 리소스 없이 통신 시스템을 신속하게 배포해야 하는 시나리오에 특히 적합합니다. 예를 들어 MANET은 기본 라우팅 프로토콜이 중요한 데이터 패킷의 보급 기능을 유지하는 데 중요한 자연 재해에서 구조 대원 간의 상호 통신 프로세스를 용이하게 할 수 있습니다. 그러나 모든 MANET의 백본, 즉 라우팅 프로토콜은 노드의 통신 범위, 고속 이동성 및 에너지 용량 제약에 의해 제한됩니다. 본 연구에서는 이러한 MANET 노드의 특성에 의존하여 데이터 전송을 위한 신뢰할 수 있는 경로를 결정하는 내결함성 임시 주문형 라우팅 프로토콜(FT-AORP)을 제안했습니다. 그 후, 발견된 경로 중 2개는 내결함성을 최대화하기 위해 원본 데이터 패킷의 복제본을 전송하는 데 사용되었습니다. 또한 OMNeT++ 네트워크 시뮬레이터를 사용하여 네트워크 노드 수, 노드 속도, 데이터 패킷 전송률의 세 가지 시뮬레이션 매개 변수에 대한 광범위한 시뮬레이션 실험을 통해 제안 시스템의 성능을 평가했습니다. 시뮬레이션 결과는 FT-AORP가 다른 기준 라우팅 프로토콜과 비교하여 패킷 전달 비율을 크게 개선하고 종단 간 지연을 줄였으며 전송 경로의 더 높은 잔류 에너지 수준을 유지했음을 보여주었습니다. Mobile ad hoc networks (MANETs) are particularly suited for scenarios that demand rapid deployment of a communication system without any existing network resources. For instance, a MANET can facilitate the intercommunication process between members of a rescue party in a natural disaster, where the underlying routing protocol is crucial to maintaining the dissemination capability of data-critical packets. However, the backbone of every MANET, i.e., their routing protocol, is limited by the communication range of nodes, their high-speed mobility, and the capacity constraints of energy. This study proposed a fault-tolerant ad hoc on-demand routing protocol (FT-AORP) that relies on these characteristics of MANET nodes to determine reliable paths for data transmission. Subsequently, two of the discovered paths were used to transmit the duplicates of an original data packet to maximize fault tolerance. Further, using the OMNeT++ network simulator, the performance of the proposed system was evaluated through extensive simulation experiments against three simulation parameters: the number of network nodes, node speed, and data packet sending rate. The simulation results demonstrated that FT-AORP greatly improved the packet delivery ratio, reduced end-to-end delay, and maintained a higher residual energy level of the transmission path, compared to other baseline routing protocols.

DSVM Methods for Common-Mode Voltage Reduction and Input Power Factor Compensation of Matrix Converters : 매트릭스 컨버터의 공통모드 전압 저감과 입력 역률 보상을 위한 공간벡터변조기법

Nguyen Minh Hoang 울산대학교 2010 국내박사

Matrix converter has become a modern converter which fulfills all the requirements of the conventionally used rectifier/ dc link/ inverter structures. Furthermore, matrix converter has many advantages that include bidirectional power flow capability, a long lifetime, a size reduction, a controllable input power factor and sinusoidal input currents. This research analyzed the limitations of matrix converter which exist in the conventional DSVM method and the effects of input filter as well. In order to overcome these problems, we proposed several DSVM methods for matrix converter. For common-mode voltage (CMV) reduction, we proposed a DSVM method based on using a couple of appropriate active switching configurations (SC) instead of zero SC, the CMV reduction obtained was 42% beside the lower THD compared to those of the conventional DSVM method. For the power factor correction purpose, two proposed DSVM method (I and II) under considering the controllable power factor capability were presented. The new PWM switching pattern was developed in the proposed DSVM method I with voltage transfer ratio less than 0.866. Many analyses of the proposed DSVM method II with voltage transfer ratio less than 0.5 were carried out in terms of common-mode voltage reduction, minimum switching loss and better power quality compared to those obtained in the conventional DSVM method. In order to overcome the low power factor in case of the low output conditions, two input power factor compensation algorithms were proposed to compensate the effects of input filter for the higher input power factor at power supply. Finally, the most recommended application of matrix converter was successfully implemented in overhead crane control based on industrial networks. Simulation and experimental results were provided to verify the feasibility and the effectiveness of the proposed theories.

Wide-Tuning Range Ring type VCOs Using 0.13-μm CMOS Process

Nguyen Minh Hoang 강원대학교 대학원 2010 국내석사

This thesis presents the design of voltage controlled oscillator (VCO) that consists of four-stage delay cells with a new differential topology using a 0.13μm CMOS process. It utilizes dual-delay path or feed-forward technique to achieve high operation frequency and widening the tuning range. In our work, VCO is designed from hand calculation and verified by simulation on the schematic scheme. The output frequency ranges from 3.8GHz to 8.1GHz with control voltage varying from 0 to 1V and thus the tuning range is approximately 75%. The simulated result of phase noise is -101.2dBc/Hz @ 1MHz offset from the center frequency of 5.75GHz. The total power dissipation is 63.6mW at 0.4V control voltage. The proposed ring type VCOs is compared with other recently reported ring oscillators by figure of merit (FOM) to evaluate performance 이 논문은 0.13um CMOS 공정을 이용하여 새로운 차동 구조의 4 단의 지연 셀을 가지는 전압 제어 발진기의 설계를 제안한다. 고 주파수 동작과 넓은 주파수 가변 범위를 가지기 위해 두 개의 지연 경로 또는 피드-포워드 기술을 이용하였다. 실제 계산을 통해 설계를 하였으며 회로와 레이아웃 레벨에서의 시뮬레이션을 통해 검증하였다. 시뮬레이션 결과 설계된 전압 제어 발진기는 1.2V 공급 전압에서 5.75GHz 의 중심 주파수로부터 3.8GHz - 8.1GHz 까지의 발진 범위를 가지며 가변 범위는 75%이다. 위상 잡음은 중심

Supplementing Hongjam recovered behavioral and biochemical defects in APOE-/- mice

Hoang, Nguyen Minh Anh 한림대학교 대학원 2024 국내석사

홍잠 섭취에 의한APOE -/- 마우스의 행동 및 생화학적 결함 회복 2023. 석사학위논문 호앙 유엔 민 안 노화생명과학과 지도교수 고 영 호 일반적으로 경도인지장애(MCI)는 노화과정 중에 나타나는 정상적 기억력 감퇴와 기억력이 과도하게 손상되면서 나타나는 질병인 치매(dementia) 사이의 과도기 단계이다. 현재 치매 치료에 허가된 의약품들은 원치 않는 심각한 부작용이 보고 돼서, 아직도 치매를 대처하는 최선의 방법은 노화에 따른 기억력 저하로 인한 MCI로 진행을 억제하거나, 다시 정상으로 회복 시킬 수 있는 방법의 개발이 필요하다. 누에의 숙잠을 쪄서 제조하는 홍잠(HJ)은 최근의 연구결과에 따르면 기억력 개선 효과와 간 기능개선 효과와 건강수명 증대 효과가 있음이 밝혀졌다. HJ의 영양성분은 70% 이상의 단백질과 15%의 지질과 다양한 종류의 식물 유래 화합물들과 미네랄 등이 다량으로 함유되어 있다. 하지만, HJ이 유전적인 문제에 의하여 발병하는 고 지혈증과 혈관성 치매의 발병에 영향을 줄 수 있는지 여부는 연구되지 않았다. 본 연구에서는 APOE Knock Out (APOE-/-)마우스 모델을 이용하여 다양한 기억력 및 운동 능력 비교 연구와 생화학적인 연구를 진행하여 HJ가 어떠한 영향을 미치는 지에 대한 연구를 진행했다. 이전의 연구에 따르면, APOE-/- 암컷 쥐는 정상 대조군에 비해 유의하게 높은 치매 발병 위험과 고지혈증을 가지고 있다. 하지만, 본 연구에서 HJ를 섭취한 암컷 APOE-/- 쥐는 운동 능력과 공간 기억력과 사교성 및 새로운 자극에 대한 호기심이 일반 사료 섭취 암컷 APOE-/- 쥐와 비교하면 통계적으로 유의하게 향상됐다. 또한 HJ 섭취는 콜레스테롤, 젖산, 중성지방 수치에도 긍정적인 영향을 주었다. 그리고, HJ를 섭취한 실험동물 뇌 조직은 미토콘드리아 활성이 증가되어 있었다. 이 결과는 HJ의 섭취가 뇌를 포함한 다양한 조직과 기관의 마이토콘드리아의 활성을 증진시켜서 기능을 활성화 시킴으로 달성되는 것으로 추측된다. 본 논문의 연구 결과를 보면, HJ 섭취에 따른 효과는 단일 성분에 의해 발생하는 것이 아니라 단백질, 지방산, 미네랄, 식물 유래 화합물들의 상호작용에 의한 것으로 사료된다. 본 논문은 APOE의 유전적 결함에 의하여 발병하는 고지혈증과 혈관성 치매는 HJ을 섭취함으로써 질환의 발병을 억제하고 진전을 늦출 수 있음을 보여주므로 이에 대한 추가 연구가 필요하다. 키워드 : 알츠하이머병, APOE-/- 마우스 모델, HongJam, 행동검사, 효소분석, 미토콘드리아 복합체, ATP Mild cognitive impairment (MCI), in general, increases the risk of developing dementia and is often considered a transitional phase from healthy cognitive aging to unhealthy dementia. Due to unwanted side effects of commercial medicines, developing new treatments that can suppress memory deterioration during MCI is not only of high interest but a crucial necessity for a healthy aging. HongJam (HJ) contains a remarkable amount of nutrients and phytochemicals which are not only necessary for the nervous system function but also control blood cholesterol and glucose levels. However, it is not still clear whether HJ can influence the late-onset of AD as well as high triglycerides level. In order to determine the effectiveness of HJ food on cognition in the APOE-/- mice, several behavioral tasks and enzyme assays are used, which differ in sensitivity to detect cognitive impairments for this strain. Such rigorous assessments give insights into the most sensitive influence on the subject’s cognitive deficits based on the effect of HJ food concentration. Although female mice exhibited significantly greater risk of developing AD and greater dementia compared to normal controls, this study observed an improvement in female APOE-/-mice fed with HJ on better spatial memory, sociability and curiosity for new stimulations along with improved motor function. Moreover, HJ food also showed the positive effects on cholesterol, lactate and triglycerides level. In addition, it is possible that HJ proteins might increase mitochondrial activity to improve lifespan of the test subjects. Although the precise underlying mechanism and the details of the active substances in HJ food remained elusive, this herbal medicine might offer a novel approach for the prevention of dementia disease. The enhancing effects of HJ food are not caused by a single component, but by the combination of proteins, fatty acids, minerals, and phytochemicals interaction together which activate various signaling mechanisms including the mitochondrial activity increase in brain tissues. As a whole, in the future, it is necessary to study whether HJ may have precise mechanism in therapeutic effect of APOE-/- mice with clear AD symptoms and high triglycerides levels.

Improving the short-range flood forecasting capability of a coupled meteorological and hydrological model

Nguyen, Hoang Minh Sejong University 2019 국내박사

Flooding is one of the most serious and frequently occurring natural disasters in many regions around the world. In particular, given climate change, the impacts of flooding increase over time. To mitigate the damages caused by floods, flood forecasting is required to support water resource managers. Unfortunately, the accuracy of such forecasts remains limited because of the uncertainties that arise from various sources. These uncertainties may result in ineffective mitigation of flood damages. The coupling of meteorological and hydrological models is one of the most common methods for predicting streamflow. In this method, the capability of the streamflow predictions depends strongly on the performance of numerical weather prediction (NWP) model. However, the outputs of NWP models, especially quantitative precipitation forecasts (QPFs), are usually only somewhat skillful. This leads to the requirements for increasing the skills of QPFs. The improvements in QPFs are not required only in term of qualitative and quantitative skills but they also must involve the increase in probabilistic skill that is very valuable for the warnings of heavy rainfall and flooding. Because deterministic forecast may not really useful in the probabilistic forecast, ensemble predictions, which could address this problem, are thus becoming increasingly common and widely applied. Ensemble predictions allow analyzing qualitative and quantitative skills by using ensemble mean and probabilistic skill by considering all of their members. Ensemble QPFs can be derived from multiple runs of NWP model and statistically post-processing deterministic forecasts. By dint of the quick operation in ensemble streamflow prediction and non-requirement of high computational resources, the latter has been a subject of interest in several previous studies. However, the methods proposed in previous studies often require a large amount of historical forecast data for estimating parameter set. Hence, they are not suitable for applying to deterministic NWP models that have been launched operationally for a short time. Besides, these methods were only applied to medium-range (3-15 days) forecasts with accumulated rainfall in several hours or seasonal forecasts with daily rainfall totals. The applications for short-range (12-72 hours) forecasts with hourly rainfall have not been considered because hourly rainfall has a more greatly skewed distribution and higher intermittency of precipitation. Even though a method that could address these issues was proposed in a study, its effectiveness was not high due to the strong dependence of generated ensemble predictions on deterministic forecasts. Therefore, this study was conducted to propose a method, which could address the limitations of previous studies, for improving the capability of short-range rainfall and flood forecasts using a coupled meteorological and hydrological model. Three individual approaches including ensemble generation, blending, hydrological correction and real-time correction (Kalman filter technique) are developed and applied. To generate the ensemble precipitation prediction from single-valued rainfall forecast, the qualitative and quantitative skills are firstly evaluated at previous time step window and are then used to define the perturbation weights of QPF issued at current time by looking up a table. Ensemble QPF, which is generated as a kind of Monte-Carlo simulation, is highly dependent on the performance of deterministic NWP model because its members oscillate around the deterministic rainfall forecast. A blending technique is applied to mitigate this dependence by taking the advantages of radar-based rainfall prediction which is the best forecast for very short-range (6 hours ahead). This technique only reduces the rainfall intensity errors without considering the spatial rainfall errors (mis-location and extent) that have considerable contributions in the accuracy of rainfall and flood forecasts. Thus, a rainfall correction method that may involve the spatial rainfall errors is proposed. Target basin is located into different zones to account for the errors inside the basin and contiguous areas. Forecast errors in each zone basin are evaluated at previous time step window and then displaced to the target basin by performing a hydrological correction. Next, the forecast errors are continuously predicted and updated in real-time by using a Kalman filter technique and are then used to verify the rainfall forecast of each ensemble member; this process is called Kalman correction. Finally, each ensemble member is forced in turn as input for a rainfall - runoff model to obtain ensemble streamflow prediction. To validate the performance of the proposed method, it was applied to improve the capability of short-range rainfall and flood forecasts for the two flood events that occurred in 2013 and 2016 on the Yeongwol watershed using a coupled Local Data Assimilation and Prediction system (LDAPS) and Sejong University Rainfall - Runoff (SURR) model. Ensemble precipitation predictions were generated from the rainfall forecasts of the deterministic LDAPS model and were then blended with the McGill algorithm for precipitation nowcasting by Lagrangian extrapolation (MAPLE) rainfall predictions to produce hybrid rainfall forecasts. These hybrids continued to be verified by applying the rainfall correction method that involves the forecast errors caused by mis-location and extent. Eventually, each ensemble member of the verified hybrids was used to driver the SURR model to obtain the ensemble streamflow predictions on the watershed. The performance of the method was evaluated in term of qualitative (Proportion Correct (PC), Critical Success Index (CSI), Bias ratio, and False Alarm Ratio(FAR)), quantitative (Root Mean Square Error (RMSE), Mean Error (ME), Mean Absolute Error (MAE), Correlation Coefficient (CC), Model Efficient (NSE), and Absolute Relative in Volume (AREV)), and probabilistic (Brier score) skills. The results showed that the capability of rainfall and flood forecasts using the coupled model was improved sustainably step by step and exhibited the best skills after applying the final step that is Kalman correction. For rainfall forecast, the values of PC, CSI, and Bias averaged over total lead time were improved dramatically by 59%, 60%, and 106%, respectively, for flood event in 2013; 39%, 40%, and 257%, respectively, for flood event in 2016, whereas the improvements in RMSE, ME, MAE, and CC were 32%, 74%, 16%, and 32%, respectively, for flood event in 2013; 15%, 97%, 8%, and 33%, respectively, for flood event in 2016. The probabilistic skill increased dramatically with values of Brier score decreasing by 48% and 37% for flood events in 2013 and 2016, respectively. For flood forecasting, the improvements in RMSE, NSE, AREV, and ME were 30%, 63%, 24%, and 103%, respectively, in 2013; 42%, 42%, 53%, and 134%, respectively, in 2016. The success of this case study proved the viability of the methodology proposed in this study. A notable point is the application of the linear Kalman filter technique for correcting the rainfall forecast of each ensemble member, which may not produce the highest effectiveness of the rainfall correction method. Further study would be conducted to investigate the performance of non-linear ensemble Kalman filter technique for improving the effectiveness of the method.

A Study on Indirect Vector AC Current Control Method Using Matrix Converter Fed Inductin Motor : 매트릭스 컨버터를 사용한 유도전동기 간접 백터제어를 위한 전류제어기법에 관한 연구

HOANG, NGUYEN MINH 울산대학교 대학원 2005 국내박사

Three-phase Matrix Converter has become an effective substitution for the conventional converter module due to generation of the feasible variety of frequency and amplitude output voltage without energy storage elements besides the advantages such as unity input power factor at the power supply and the regeneration capability. For that advances, this master thesis describes works investigated the operation of the matrix converter fed induction motor using a first proposed indirect vector ac current control method, and then a second modified method respectively. Some simulation and experiment results are carried out to validate the effectiveness of the two-proposed methods as compared with the classical method using voltage source inverter. Three-phase Matrix Converter has become an effective substitution for the conventional converter module due to generation of the feasible variety of frequency and amplitude output voltage without energy storage elements besides the advantages such as unity input power factor at the power supply and the regeneration capability. For that advances, this master thesis describes works investigated the operation of the matrix converter fed induction motor using a first proposed indirect vector ac current control method, and then a second modified method respectively. Some simulation and experiment results are carried out to validate the effectiveness of the two-proposed methods as compared with the classical method using voltage source inverter.

(The) effect of an antigalvanic reduction of silver on gold for the stability of a self-assembled alkanethiol monolayer and chemical lift-off lithography

Nguyen Minh Hoang Garduate School, Korea University 2019 국내석사

The effects of silver anti-galvanic reduction (Ag AGR) on the structure of alkanethiol self-assembled monolayers (SAMs) on gold with regard to the ordered structure and surface coverage were studied using electrochemical methods. In this paper, we report the structure changes as well as the chemical lift-off lithography (CLL) performance of SAMs dependent on chain lengths, namely 1-butanethiol, 6-mercapto-1-hexanol and 11-mercapto-1-undecanol. Cyclic voltammetry of redox probe and linear sweep voltammetry of reductive desorption demonstrated that the structure of SAMs with Ag AGR pretreatment are more ordered resulted from higher surface coverage and stronger molecules interaction. Meanwhile, influence of Ag AGR on the CLL efficiency behaved differently depending on the SAM chain lengths in both electrochemistry and AFM imaging. Nevertheless, the formation of alkanethiol SAMs on Ag AGR/Au has enabled a facile method which possesses a great potential on the quality control of the SAMs system.