발암억제와 유산균

배형석 中央大學校 遺傳工學硏究所 1993 遺傳工學硏究論集 Vol.6 No.1

This study was to review recent reports on antitumor activity of lactic acid bacteria including the relationship among the gut microflola, nutritional factors and carcinogenesis in human and animal models. The fecal bacterial enzymes, β-glucuronidase, nitroreductase, and azoreductase were shown to play an important role in the incidence of colon cancer. These were increased on a Western(high fat and animal protein) diet, but reduced by consumption of Lactobacilli and Bifidobacteria with the diet. High consuming(≥225g/day) of fermented milk products, even the people on a Western diet were epidemiologically revealed to be at low risk for colon and breast cancer. This antitumor effect is augmented by reducing production of harmful bacteria enzymes such as β-glucuronidase, nitroreductase, and azoreductase due to altering intestinal flora metabolic activity. In animal models having tumors, Lactobacilli cultures also significantly suppressed the growth of Sarcoma, Leukemia, and Carcinoma. The antitumor effect of lactic acid bacteria are supposed to be host- mediated and dependent on the activation of non-specific effector cells such as macrophages or natural killer cells.

항암제와 Lactobacillus casei HY2782의 병용투여에 의한 항암효과의 증강

윤상군,배형석,김경태,백영진 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

S-180 복수암을 유발시킨 생쥐에 대한 BCG, OK-432와 LC2782의 항암효과 비교실험 결과 LC2782를 투여한 생쥐의 평균 생존율은 192%(p<0.001)였으며, OK-432 와 BCG를 투여한 생쥐의 평균 생존율은 각각 141%(p<0.01), 112%였다. 3LL 고형암을 유발시킨 생쥐에 대한 BCG , OK-432와 LC2782의 항암효과 비교실험 결과, LC2782를 투여한 생쥐의 암 억제율은 62%(p<0.05)였으며, OK-432 와 BCG를 투여한 실험군의 암억제율은 90%(p<0.05)와 76.2%(p<0.05)로서 OK-432가 가장 높은 암억제 효과를 나타냈다. 또한 S-180 복수암을 유발시킨 5-Fu와 CP를 단독 투여한 경우의 평균 생존율은 각각 115%와 99%였으나, 5-Fu와 CP를 LC2782 와 병용 투여한 경우의 평균 생존율은 각각 226%(p<0.001)1와 244%(p<0.00)1를 나타내어 단독 투여시보다 2배 이상 생존율이 증가하였다. 3LL 고형암을 유발시킨 생쥐에 5-Fu 와 CP를 LC2782와 병용 투여한 경우의 암억제율은 99.3%(p<0.05)와 73%(p<0.025)였다. Augmentation of antitumor activity of antitumor drugs in combination with Lactobacillus casei Hy2782 (LC2782) was studied against Sarcoma-180 (S-180) and Lewis lung carcinoma (3LL). Antitumor drugs used in this study were 5-fluorouracil (5-Fu) and cyclophosphamide (CP). The prolongation effect of LC2782 on the life span of mouse intraperitoneally implanted with S-180 was stronger than that of OK-432 and BCG, while the inhibitory effect of OK-432 and BCG on the growth of 3LL solid tumor was a little stronger than that of LC2782. Average survival rates of mice administrated LC2782, OK-432 and BCG were 192%, 141%, and 112%, respectively, when that of the control was 100%. Intralesional administration of 5-Fu, CP, 5-Fu+LC2782 and CP+LC2782 resulted in 93%, 69%, 99% and 73% inhibition rates against 3LL solid tumor proliferation. The combination therapy of 5-Fu or Cp with LC2782 significantly prolonged the life span of S-180-inoculated ICR mice. Average survival rates of mice administrated 5-Fu and CP alone were 115% and 99%. Furthermore, survival rates of mice administrated 5-Fu and CP in combination with LC2782 were 226% and 244%, respectively.

유산균 투여가 건강한 성인의 분변미생물 및 부패산물 생성에 미치는 영향

신명수,김용재,배형석,백영진 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

유산균의 장내균총과 부패산물 생성에 미치는 영향을 알아보기 위하여, 건강한 성인 남녀 6명의 지원자에게 Lactobacillus acidophilus 와Bifidobacterium longum으로 제조한 유산균분말(각각 1.5×10 exp (9) cells)을 하루에 2회씩 2주동안 섭취하도록 하였다. 그리고 지원자 분변으로부터 균총수 변화와 부패산물인 암모니아, indole, skatole, p-cresole량을 측정하였다. 유산균의 투여 기간중에 분변내 bifidobacteria의 수가 8.78±0.39(log cfu/g feces±S.D)에서 9.27±0.29로 증가하였고(p<0.05) 투여 중지후에는 약간 감소하였으며, lactobacilli의 수는 6.15±0.80에서 6.76±0.48로 증가하였다(p<0.05). Enterococcus 균수는 투여 기간중에 6.66±0.80에서 7.72±0.40으로 증가하였다가 투여 중지후에는 감소하는 경향을 보였다. 그리고 혐기성균인 Bacteroides는 투여중에 8.45±0.34에서 9.15±0.21으로 증가하였으나(p<0.01), 유해균주로 알려진 Closridium(lecithinase negative)과 Staphylococcus 균수는 현저히 감소하였다. 그러나, 유산균 투여 실험기간인 6주 동안 분변내 암모니아, indole, skatole, p-cresole의 유의적인 농도변화는 없었다. To investigate the effects of lactic acid bacteria administration on fecal microflora and putrefactive metabolites in human being, Lactobacillus acidophilus and Bifidobacterium longum powder (1.5×10 exp (9) cells, respectively) was administrated to six healthy volunteers (average 28 years old) twice a day for 2 weeks. During the administration of lactic acid bacteria, the numbers of bifidobacteria, lactobacilli, and enterococci in feces were increased significantly, whereas those of Staphylococcus and lecithinase-negative Clostridium were decreased considerably. In addition, a number of anaerobic Bacteroides were increased. However, the contents of fecal ammonia and putrefactive metabolites (indole, skatole, p-cresole) were not changed during the administration.

CMOS소자를 위한 Ni Silicide의 Dopant에 따른 영향분석

배미숙(Mi-Suk Bae),지희환(Hee-Hwan Ji),이헌진(Hun-Jin lee),안순의(Soon-Eui Ahn),박성형(Sung-Hyung Park),이기민(Key-Min Lee),이주형(Joo-Hyoung Lee),왕진석(Jin Suk Wang),이희덕(Hi-Deok Lee) 대한전자공학회 2002 대한전자공학회 학술대회 Vol.2002 No.6

심한 근육염이 동반된 Mumps 바이러스 감염증 1예

김석배,허재형,홍석균,신정우,김인호,배현주,조정희,이주연,박종원 대한감염학회 1999 감염 Vol.31 No.3

생쥐에서 Sarcoma 180 및 Lewis Lung Carcinoma에 대한 Lactobacillus casei의 항암 효과

배형석,백영진,윤영호 한국산업미생물학회 1993 한국미생물·생명공학회지 Vol.21 No.3

마우스에서 sarcoma 180(S-180)과 Lewis lung carcinoma(3LL)를 유발시키고 유산균의 항암 활성을 정확하게 측정할 수 있는 in vivo 암 모델을 정립하고 이 암들을 치료하기에 적합한 가열 살균한 Lactobacillus casei YIT 9018(LC9018)의 최적 투여조건과 그 항암 효과를 검토하였다. S-180 복수암, S-180 고형암, 3LL의 3가지 in vivo 암 모델이 확립되었다. S-180 복수암이 유발된 마우스들의 평균 생존일은 17±1일이었다. 암세포(2×10^6 cells)를 서혜부 피하에 접종한 후 21일이 경과하였을 때 S-180 고형암과 3LL에 걸린 마우스들로부터 적출한 암괴의 무게는 각각 1.29±1.08 g, 3.01±1.64g이었다. S-180 복수암, S-180 고형암, 3LL에 대한 항암효과를 나타내는 가열 살균한 LC9018의 최적 투여량은 공히 100 ㎍/mouse였다. 그리고, LC9018의 항암활성은 25∼100 ㎍ 범위에서 유산균 투여량에 비례하여 증가하였다. LC9018 100㎍을 마우스의 구강, 정맥, 복강, 암 유발 부위에 투여하였을 때, 암 유발 부위에 투여한 것이 이들 종양들의 성장을 가장 현저하게 억제하였다. LC9018 100 ㎍을 S-180 복수암에 걸린 ICR 마우스의 복강에 투여하였을 때, 암세포 접종 후 50일 동안 마우스의 생존율(T/C%)은 185%로서 대조군(100%)에 비하여 거의 2배로 증가하였다. LC9018 100 ㎍을 S-180 고형암과 3LL에 걸린 ICR 마우스의 우측 서혜부에 피하 투여하였을 때는, 암세포 접종 후 21 일까지 종양 성장에 대한 억제율은 각각 93%, 97%였다. 그리고 가열 살균한 LC9018은 S-180과 3LL 세포에 직접적으로 작용하는 독성 물질을 갖고 있지 않는 것으로 확인되었다. Antitumor activity of Lactobacillus casei YIT 9018(LC9018) was studied in mice by using sarcoma 180(S-180) and Lewis lung carcinoma (3LL). Following the eatablishment of in vivo tumor models such as ascites form S-180, solid form S-180 and 3LL for estimating antitumor activity of Lactobacilli, optimal dose and injection route of heat-killed LC9018 for suppression of local tumor were examined. Administration of 100 ㎍/mouse of LC9018 significantly inhibited the growth of ascites form S-180, solid form S-180 and 3LL. The antitumor activity of LC9018 increased in proportion to the amount of administration at a dose range of 25∼100㎍/mouse. Intralesional injection of LC9018 showed the greatest inhibition against the growth of all these tumors among intralesional, intravenous, intraperitoneal, and oral administrations. Heat-killed LC9018 was found not having cytotoxic substance which direct growth inhibition against S-180 and 3LL in vitro. These results suggested that the antitumor activity of LC9018 may be host-mediated.

A study on the immune response of horses bred against respiratory diseases vaccination in Jeju island

( Suk Jae Park ),( Tae Jun Kwon ),( Jae Ho Bae ),( Min Soo Kang ),( Huun Seok Chae ),( Jae Hoon Woo ),( Seol Hwa Park ),( Hyoung Seok Yang ),( Wan Shol Kang ),( Jin A Ko ),( Nam Young Kim ) 한국예방수의학회(구 한국수의공중보건학회) 2015 예방수의학회지 Vol.39 No.4

Viral respiratory infections are common in horses, notably equine herpesvirus infection and equine influenza, which primarily initiate secondary bacterial respiratory infections such as strangles caused by Streptococcus equi equi. A decline in the production of stallions has been associated with these respiratory diseases leading to adverse financial implications. This study investigated the antibody responses against respiratory diseases in horses from Jeju Island a year after vaccination. A low level of equine herpesvirus type 1 (EHV-1) (11.36%) antibodies was detected from stallions, however a high level of EHV-4 (95.84%) antibodies was detected from horses without vaccination against this infection suggesting that EHV-4 is ubiquitous in this horse population. In case of equine influenza, ranch stallions showed low positive rate (12.06%) whereas stallions from Subtropical Livestock Research Institute displayed higher positive rate (81.32%). Antibody responses against equine influenza and strangles revealed positive rates of 26.32% and 55.12%, respectively. These findings may draw attention towards the importance of developing an improved disease prevention and/or immunization program that will effectively control respiratory diseases in horses.

Genetic Analysis of Complement Component 9 (C9) Polymorphisms with Clearance of Hepatitis B Virus Infection

Bae, Joon Seol,Pasaje, Charisse Flerida A.,Park, Byung Lae,Cheong, Hyun Sub,Kim, Jeong-Hyun,Park, Tae Joon,Kim, Jason Yongha,Lee, Jin Sol,Koh, In Song,Lee, Hyo-Suk,Kim, Yoon Jun,Shin, Hyoung Doo Springer-Verlag 2011 Digestive diseases and sciences Vol.56 No.9

The Anti-oxidation of Ulva lactuca Fucoidan in Ovariectomy

Chun Suk Nam,Eun Kyung Park,Kum Suk Kang,Ju A Shim,Hee Jin Kim,Jin Young Lee,Hyoung Soo Ok,Sang Hun Lee,Jin Wook Kim,Nam Young Kim,Eo Jin Lee,Bae Jin Ha 한국생명과학회 2005 한국생명과학회 심포지움 Vol.45 No.-

Two-Step Planarization of ECMP and CMP for MEMS Copper Patterns

Jeong, Suk Hoon,Joo, Suk Bae,Lee, Ho Jun,Park, Boum Young,Kim, Hyoung Jae,Jeong, Hae Do Trans Tech Publications, Ltd. 2008 Materials science forum Vol.569 No.-

<P>Chemical mechanical polishing (CMP) has been used as planarization process in the fabrication of semiconductor devices. The CMP process is required to planarize the overburden film in an interconnect process by high relative velocity between head and platen, high pressure of head and chemical effects of an aqueous slurry. But, a variety of defects such as dishing, delamination and metal layer peering are caused by CMP factors such as high pressure, pad bending and strong chemical effect. The electrical energy of the electro-chemical mechanical planarization (ECMP) dissolves copper (Cu) solid into copper ions electrochemically in an aqueous electrolyte. The dissolved copper complex layer or passivation layer is removed by the mechanical abrasions of polishing pad and abrasive. Therefore the ECMP process realizes low pressure processing of soft metals to reduce defects comparing to traditional CMP process. But, if projected metal patterns were removed and not remained on whole wafer surface in final processing stage, Cu layer could not be removed by ECMP process. The two-step process consists of the ECMP and the conventional CMP used in micro patterned Cu wafers. First, the ECMP process removed several tens 'm of bulk copper on Cu patterned wafer within shorter process time than the Cu CMP. Next, residual Cu layer was completely removed by the Cu CMP under low pressure. Total time and process defects are extremely reduced by the two-step process.</P>