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Generation and characterization of monoclonal antibodies against human interferon-lambda1
홍승호,김정식,박선 대한면역학회 2008 Immune Network Vol.8 No.1
Background: Members belonging to the interferon-lambda (IFN-λ) family exert protective action against viral infection; however, the mechanisms of their action have remained elusive. To study IFN-λ biology, such as endocytosis of IFN- λ, we produced monoclonal antibodies (Abs) against human IFN-λ and examined their usefulness. Methods: We purified recombinant human IFN-λ1 expressed in Escherichia coli by using affinity columns. Then, we generated hybridoma cells by fusing myeloma cells with splenocytes from IFN-λ1-immunized mice. For evaluating the neutralizing activity of the monoclonal Abs against IFN-λ1, we performed RT-PCR for the MxA transcript. In order to study the binding activity of IFN-λ and the monoclonal Ab complex on HepG2 cells, we labeled the monoclonal Ab with rhodamine and determined the fluorescence intensity. Results: Four hybridoma clones secreting Abs specific to IFN-λ1 were generated and designated as HL1, HL2, HL3, and HL4. All the Abs reacted with IFN-λ1 in the denatured form as well as in the native form. Abs produced by HL1, HL3, and HL4 did not neutralize the induction of the MxA gene by IFN-λ1. We also demonstrated the binding of the HL1 monoclonal anbitody and IFN-λ complex on HepG2 cells. Conclusion: Monoclonal Abs against IFN-λ1 were produced. These Abs can be used to study the cellular binding and internalization of IFN-λ.
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A Case Study of Concrete Pavement Deterioration by Alkali-Silica Reaction in Korea
홍승호,한승환,윤경구 한국콘크리트학회 2007 International Journal of Concrete Structures and M Vol.1 No.1
The concrete pavement of the Seohae Highway in Korea has suffered from serious distress, only four to seven years after construction. Deterioration due to Alkali-Silica Reaction (ASR) has seldom been reported per se in Korea, because the aggregate used for the cement concrete has been considered safe against alkali-silica reaction so far. The purpose of this study is to examine the deterioration caused by an alkali-silica reaction of concrete pavement in Korea. The investigation methods included visual inspection and Automatic Road Analyzer (ARAN) analysis of surface cracks, coring for internal cracks, stereo microscopic analysis, scanning electronic microscope (SEM) analysis, and electron dispersive X-ray spectrometer (EDX) analysis. The results are presented as follows: The crack pattern of the concrete pavement in Korea was longitudinal cracking, map cracking or D-cracking. Local areas of damage were noticed four to five years after construction. The cracks started from edges or joints and spread out to slabs. The most intensive cracking was observed at the intersection of the transverse and longitudinal joints. Where cracking was the most intense, pieces of concrete and aggregate had spalled away from top surface and joint interface area. The progress of deterioration was very fast. The reaction product of alkali-silica gel was clearly identified by its generally colorless, white, or very pale yellow hue seen through a stereo optical microscopy. The typical locations of the reaction product were at the interface between aggregate and cement paste in a shape of a rim, within aggregate particles in the cracks, and in the large void in the cement paste. Most of the white products were found at interface or internal aggregates. SEM and EDX analysis confirmed that the white gel was a typical reaction product of ASR. The ASR gel in Korea mainly consisted of Silicate (Si) and Potassium (K) from the cement. The crack in the concrete pavement was caused by ASR. It seems that Korea is no longer safe from alkali-silica reaction.
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홍승호,김영리 한국유전학회 2016 Genes & Genomics Vol.38 No.9
Apolipoprotein C3 (ApoC3) plays a regulatory role in triglyceride (TG) metabolism. The higher level of TG can be a cause in pathogenesis of the vascular diseases or metabolic syndrome (MetS). In this study, we examined the associations of ApoC3 polymorphisms (-482C[T rs2854117 and 3238G[C rs5128) with Korean MetS patients. A total of 835 subjects were investigated, including 320 patients with MetS and 515 healthy subjects. The genotype analysis of the ApoC3 polymorphisms was performed by polymerase chain reaction-restriction fragment length polymorphism methods. Of the two polymorphisms studied, we observed a significant difference in the -482C[T polymorphism between the MetS and control groups. The TT genotype of the -482C[T polymorphism was associated with increased risk for MetS, compared with the controls (OR 1.627, 95 % CI 1.075–2.463, P = 0.021). The association was female-specific. No associations were found for the risk of MetS in the 3238G[C polymorphism. Haplotypes composed of two polymorphisms, however, were associated with MetS susceptibility in only male group. The 3238G[C polymorphism was significantly associated with TG levels (P = 0.013). Our data suggest that the ApoC3 -482C[T polymorphism is associated with increased MetS susceptibility in the Korean population.
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‘우리 몸의 구조와 기능’ 애니메이션 제작을 활용한 STEAM 프로그램 개발 및 적용 효과
홍승호,이상호 한국초등과학교육학회 2019 초등과학교육 Vol.38 No.2
The purpose of this study is to investigate the effect of STEAM program on students’ academic achievement, creative problem-solving ability, scientific inquiry ability and scientific interests of elementary school students. For this, we developed a STEAM program to utilize animation production about ‘structure and function of the our body’ unit in 5th grade. The STEAM program was developed for a total of 9 sessions and was applied to the experimental group. The theoretical lesson of the 2009 revised curriculum was consisted of 11 sessions and was applied to the comparative group. The results of study showed significant differences of the students’ academic achievement, creative problem-solving ability to the experimental group participating in the STEAM program and also showed significant effects in the domains of subject contents and teacher preference, which are the areas of scientific interests compared to the comparative group. This means that the STEAM program to utilize animation production about ‘structure and function of the our body’ developed in consideration of the characteristics of elementary students positively influenced the academic achievement, creative problem-solving ability and scientific interests of elementary school students. In the future we need to develop STEAM programs more variously that utilize animation production and that it needs to be applied to elementary schools.
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초등과학 생명영역의 생식 및 유전 분야에 대한 오개념 분석
홍승호 한국초등과학교육학회 2003 초등과학교육 Vol.22 No.3
The purpose of this study is to survey elementary students' misconception on reproduction and genetics unit in biological science. It is also to provide basic data for the effective teaching strategies which help students have the appropriate scientific conceptions. In order to investigate students' misconceptions, 19 questions were developed based on the analysis of science curriculum for elementary students. The questionnaire was used for 515 elementary students of 6th pade in Jeju-city and Bukieiu-gun, Jeju-Do. 1'test was perfDrmed to do frequency comparison by gender and counties. Analysis of variance was also used to investigate significant differences among the reasons of conception selection. Among 19 questions, 5 questions in plants and 4 questions in animals were appeared extent of misconception over 50%. The forming causes of misconception were mainly influenced by textbook and teacher. The reasons of conception selection showed statistically significant differences between right conception and misconception in 5 questions. According to the gender, the selection reasons of 3 questions were significantly different between male and female students. d students living Jeiu-ci and Beiu-gun show핀 significant differences in the selection reasons of 2 questions. Therefore, it was concluded that this study may help teachers reconsider elementary students' conceptions for reproduction and genetics and to be successful in the leaching-learning processes.
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