角膜潰瘍의 治療

洪承浩 藥業新聞社 1981 (月刊) 醫藥情報 Vol.1981 No.7

콘크리트용 골재 알칼리-골재 반응 KS F 2545 화학법과 ASTM C 1260 촉진법 비교고찰

홍승호,한승환,윤경구 한국도로학회 2007 한국도로학회 학술대회 발표논문 초록집 Vol.2007 No.10

Generation and characterization of monoclonal antibodies against human interferon-lambda1

홍승호,김정식,박선 대한면역학회 2008 Immune Network Vol.8 No.1

Background: Members belonging to the interferon-lambda (IFN-λ) family exert protective action against viral infection; however, the mechanisms of their action have remained elusive. To study IFN-λ biology, such as endocytosis of IFN- λ, we produced monoclonal antibodies (Abs) against human IFN-λ and examined their usefulness. Methods: We purified recombinant human IFN-λ1 expressed in Escherichia coli by using affinity columns. Then, we generated hybridoma cells by fusing myeloma cells with splenocytes from IFN-λ1-immunized mice. For evaluating the neutralizing activity of the monoclonal Abs against IFN-λ1, we performed RT-PCR for the MxA transcript. In order to study the binding activity of IFN-λ and the monoclonal Ab complex on HepG2 cells, we labeled the monoclonal Ab with rhodamine and determined the fluorescence intensity. Results: Four hybridoma clones secreting Abs specific to IFN-λ1 were generated and designated as HL1, HL2, HL3, and HL4. All the Abs reacted with IFN-λ1 in the denatured form as well as in the native form. Abs produced by HL1, HL3, and HL4 did not neutralize the induction of the MxA gene by IFN-λ1. We also demonstrated the binding of the HL1 monoclonal anbitody and IFN-λ complex on HepG2 cells. Conclusion: Monoclonal Abs against IFN-λ1 were produced. These Abs can be used to study the cellular binding and internalization of IFN-λ.

길

홍승호 고요아침 2017 한국동시조 Vol.- No.7

A Case Study of Concrete Pavement Deterioration by Alkali-Silica Reaction in Korea

홍승호,한승환,윤경구 한국콘크리트학회 2007 International Journal of Concrete Structures and M Vol.1 No.1

The concrete pavement of the Seohae Highway in Korea has suffered from serious distress, only four to seven years after construction. Deterioration due to Alkali-Silica Reaction (ASR) has seldom been reported per se in Korea, because the aggregate used for the cement concrete has been considered safe against alkali-silica reaction so far. The purpose of this study is to examine the deterioration caused by an alkali-silica reaction of concrete pavement in Korea. The investigation methods included visual inspection and Automatic Road Analyzer (ARAN) analysis of surface cracks, coring for internal cracks, stereo microscopic analysis, scanning electronic microscope (SEM) analysis, and electron dispersive X-ray spectrometer (EDX) analysis. The results are presented as follows: The crack pattern of the concrete pavement in Korea was longitudinal cracking, map cracking or D-cracking. Local areas of damage were noticed four to five years after construction. The cracks started from edges or joints and spread out to slabs. The most intensive cracking was observed at the intersection of the transverse and longitudinal joints. Where cracking was the most intense, pieces of concrete and aggregate had spalled away from top surface and joint interface area. The progress of deterioration was very fast. The reaction product of alkali-silica gel was clearly identified by its generally colorless, white, or very pale yellow hue seen through a stereo optical microscopy. The typical locations of the reaction product were at the interface between aggregate and cement paste in a shape of a rim, within aggregate particles in the cracks, and in the large void in the cement paste. Most of the white products were found at interface or internal aggregates. SEM and EDX analysis confirmed that the white gel was a typical reaction product of ASR. The ASR gel in Korea mainly consisted of Silicate (Si) and Potassium (K) from the cement. The crack in the concrete pavement was caused by ASR. It seems that Korea is no longer safe from alkali-silica reaction.

Influences of 2482C>T and 3238G>C polymorphisms of the Apolipoprotein C3 gene on prevalence of metabolic syndrome

홍승호,김영리 한국유전학회 2016 Genes & Genomics Vol.38 No.9

Apolipoprotein C3 (ApoC3) plays a regulatory role in triglyceride (TG) metabolism. The higher level of TG can be a cause in pathogenesis of the vascular diseases or metabolic syndrome (MetS). In this study, we examined the associations of ApoC3 polymorphisms (-482C[T rs2854117 and 3238G[C rs5128) with Korean MetS patients. A total of 835 subjects were investigated, including 320 patients with MetS and 515 healthy subjects. The genotype analysis of the ApoC3 polymorphisms was performed by polymerase chain reaction-restriction fragment length polymorphism methods. Of the two polymorphisms studied, we observed a significant difference in the -482C[T polymorphism between the MetS and control groups. The TT genotype of the -482C[T polymorphism was associated with increased risk for MetS, compared with the controls (OR 1.627, 95 % CI 1.075–2.463, P = 0.021). The association was female-specific. No associations were found for the risk of MetS in the 3238G[C polymorphism. Haplotypes composed of two polymorphisms, however, were associated with MetS susceptibility in only male group. The 3238G[C polymorphism was significantly associated with TG levels (P = 0.013). Our data suggest that the ApoC3 -482C[T polymorphism is associated with increased MetS susceptibility in the Korean population.

‘우리 몸의 구조와 기능’ 애니메이션 제작을 활용한 STEAM 프로그램 개발 및 적용 효과

홍승호,이상호 한국초등과학교육학회 2019 초등과학교육 Vol.38 No.2

The purpose of this study is to investigate the effect of STEAM program on students’ academic achievement, creative problem-solving ability, scientific inquiry ability and scientific interests of elementary school students. For this, we developed a STEAM program to utilize animation production about ‘structure and function of the our body’ unit in 5th grade. The STEAM program was developed for a total of 9 sessions and was applied to the experimental group. The theoretical lesson of the 2009 revised curriculum was consisted of 11 sessions and was applied to the comparative group. The results of study showed significant differences of the students’ academic achievement, creative problem-solving ability to the experimental group participating in the STEAM program and also showed significant effects in the domains of subject contents and teacher preference, which are the areas of scientific interests compared to the comparative group. This means that the STEAM program to utilize animation production about ‘structure and function of the our body’ developed in consideration of the characteristics of elementary students positively influenced the academic achievement, creative problem-solving ability and scientific interests of elementary school students. In the future we need to develop STEAM programs more variously that utilize animation production and that it needs to be applied to elementary schools.

PARTICLE COUNTER에 의한 CLEAN ROOM 계측

홍승호 대한설비공학회 1986 대한설비공학회 강연회 및 기타간행물 Vol.- No.-

압록강 하구부터 두만강 하구까지 답사기

홍승호 한국도로학회 2019 도로 : 도로학회지 Vol.21 No.1

IEC/ISA 필드버스의 우선 순위 성능 분석

홍승호,고성준,Hong, Seung-Ho,Ko, Seong-Jun 대한전자공학회 1998 電子工學會論文誌, S Vol.s35 No.10

This paper investigates the performance characteristics of the data link layer of the 1996 version of IEC/ISA fieldbus which is developed as internatioal standard of fieldbus. A discrete-event simulation model of the data link layer of IEC/ISA fieldbus is developed. Using the simulation model, this paper identifies the network parameters that influence the performance of prioritys scheme and real-time data transmission, and analyzes the delay performance of IEC/ISA fieldbus with respect to the change of network parameters. The simulation model also integrates continuous-time simulation model of a control system as application process. Using the integrated simulation model, this study investigates the effect of network-induced delay on the performance of control system. Since the simulation model developed in this study can predict the network performance, it will be effectively utilized in the design phase of control and automation systems that adopt the IEC/ISA fieldbus. 본 논문에서는 필드버승의 국제 규격인 IEC/ISA 필드버스의 1996년도 제안서를 토대로 데이터 링크 계층에 대한 성능을 시뮬레이션 기법을 통하여 해석한다. 이 연구를 위해 스케듈링 기능과 우선 순위 기능을 포함하는 데이터 링크 계층의 이산사건 시뮬레이터를 개발하였고, 이를 통하여 IEC/ISA 필드버스에서 제공하는 우선 순위 기능의 동작과 실시간 데이터의 전송 특성에 영향을 주는 네트워크 파라미터들을 파악하였다. 또한 시뮬레이션 모델을 이용하여 IEC/ISA 필드버스가 구축될 수 있는 실제의 여러 네트워크 시스템 환경에 대하여 네트워크 파라미터의 변화에 대한 IEC/ISA 필드버스의 데이터 전송 ㅣ지연 시간의 특성을 분석하였다. 본 연구를 통하여 개발된 시뮬레이션 모델에서는 또한 응용 프로세스로 제어 시스템의 연속시간 시뮬레이션 모델을 통합함으로써, 데이터 전송 지연 시간이 필드버스에 접속된 제어 시스템의 제어 성능에 미치는 영향을 조사하였다. 본 연구를 통하여 개발된 시뮬레이션 모델은 네트워크의 성능을 미리 예측 가능하게 하며, 따라서 IEC/ISA 필드버스가 도입되는 제어 및 자동화 시스템의 설계시 매우 유용하게 활용될 수 있을 것이다.