감초폴리페놀 추출효율에 있어 열처리, 에탄올 농도, 추출시간 및 용매비율이 미치는 영향 탐색

채정일,류경선,서강석,김경훈,오영균,장선식,최창원,최낙진,Chae, Jung-Il,Ryu, Kyeong-Seon,Seo, Kang-Seok,Kim, Kyung-Hoon,Oh, Young-Kyoon,Jang, Sun-Sik,Choi, Chang-Weon,Choi, Nag-Jin Korean Association of Organic Agriculture 2012 韓國有機農業學會誌 Vol.20 No.3

Effects of pretreatment and extraction conditions on total polyphenol yield from licorice root were investigated using statistical method. For pretreatment, heat treatment at $121^{\circ}C$ for 10 min was applied. Licorice root content in solvent (10, 20, and 30%) ethanol concentration (20, 40, and 60%) and reaction time (1, 2, and 3 h) were used as variables for extraction conditions. Two experiments, with heat treated and no treated licorice, were prepared with same experimental design. Box behnken design was employed and produced a total of 15 trials. Total polyphenol yield from licorice root was not affected by heat treatment. Among variables, licorice content in solvent showed most significant effect regardless of other variables (p<0.05). Finally, optimum conditions for the extraction of total polyphenol from licorice root was detected as following: 10% of licorice in solvent, 52% ethanol as solvent, 2 h of reaction time and non-heat treatment and the extraction yield from optimized condition was 17.6 mg/g licorice root.

인간 구강 편평 상피암 세포에서 Sp1단백질의 조절을 통한 Mithramycin A의 세포 사멸효과

채정일,심정현 대한암예방학회 2012 Journal of cancer prevention Vol.17 No.3

Mithramycin A (Mith A), an older chemotherapy drug, has been studied as a potential therapeutic agent that may possess anti-tumor activity as well as apoptotic effect in several cancer patients. No related research has been done on the apoptotic effect of Mith A in HN22 and HSC4 oral squamous cancer cells using the specificity protein 1 (Sp1) protein. Hence, here we investigated whether Mith A can inhibit cell growth and regulate Sp1 target proteins by suppressing levels of Sp1 protein in HN22 and HSC4 cells. As a result, oral squamous cancer cell viability was reduced and apoptotic cell death was increased by Mith A. IC50 of Mith A for 48 h treatment in the HN22 and HSC4 cells were calculated to be approximately 25 and 26 nM. Mith A significantly suppressed Sp1 protein expression levels and modulated the expression level of the Sp1 regulatory protein such as p27, p21, cyclin D1, Mcl-1, and survivin in HN-22 and HSC-4 cells. Mith A also induced apoptotic signaling molecules, including: cleavages of Bid, caspase-3, and PARP, and upregulation of Bax in HN22 and HSC4 cells. In conclusion, our results strongly suggest that Mith A regulated proliferation and mediated apoptosis through inhibition of Sp1 in oral squamous cancer cells.

도로교통 기반 통행목적별 접근성 지수를 활용한 복합개발 방향설정

채정표(Chae, Jung-Pyo),성현곤(Sung, Hyungun) SH도시연구원 2018 주택도시연구 Vol.8 No.2

The Mixed-Use Development (MXD) has been recognized not only to play a critical role in vitalizing urban diversity, but also to solve such urban problems as dependence on automobile travel and worsening traffic congestion. The purpose of this study is to deduce the directions on how to categorize as well as where to locate various MXD types, by using spatial accessibility indices by travel purpose in the Seoul metropolitan area. First, we constructed the Origin/Destination Matrix with shortest travel time and distance respectively, through GIS network analysis as a process, to calculate the spatial accessibility indices by travel purpose. Seond, based on these results, we extracted and categorized the areas wherein a certain MDX type is more feasible through factor analysis of accessibility indices by dong-level. Conclusively, seven potential MXD types were categorized and their locations were provided. Our analysis results can be contributed to deciding the directions for MXD projects in the Seoul metropolitan area in future.

실천적 추론 수업이 중학생의 자아존중감에 미치는 효과

채정현,유태명 한국가정과교육학회 2006 한국가정과교육학회지 Vol.18 No.1

This study developed practical reasoning teaching-learning material for middle school home economics education and tested its effects on middle school students' self-esteem. The type of this study was a quasi-experimental research and its independent variable was practical reasoning instruction and its dependent variable was self-esteem. The research design for this study was a pretest-posttest design. Practical reasoning instruction was given for experimental group whereas traditional instruction was given to comparison group. For measuring self-esteem, ‘self-esteem scale’ developed by Choi & Jun(1993) was used and its Cronbach' α was ranged from .69 to .84. Statistic analysis was done by mean and standard deviation for general characteristics of samples and paired t-test for testing difference of pretest-posttest. The results of this study showed that practical reasoning instruction improved middles school students' self-esteem, the general self-esteem and social self-esteem among sub-areas of self-esteem in particular. 본 연구는 중학생을 대상으로 실천적 추론을 강조한 가정과 교수학습 자료를 개발하고 현장 교사가 이 수업을 실행하여 자아존중감의 효과를 측정하여 분석하였다. 본 연구의 형태는 의사실험 연구(quasi-experimental research)로서 독립변수는 실천적 추론 가정과수업이며 종속변수는 자아 존중감이다. 본 연구의 설계는 전후검사설계로 기존의 학급을 유지하면서 실험집단에는 실천적 추론 가정과수업을 행하고 비교 집단에는 기존에 행하고 있는 가정과수업을 그대로 행하여 실험전과 후에 자아존중감을 검사를 하였다. 자아존중감을 측정하기 위하여 최보가와 전귀연(1993)이 연구 개발한 ‘자아존중감 척도’를 사용하였다. 이 조사도구의 신뢰도는 Cronbach 알파계수가 .69에서 .84의 범위 수준이었다. 일반적 경향을 알아보기 위해서 평균과 표준편차, 빈도와 백분율을 내었으며, 집단의 사전 사후 차이를 알아보기 위해서 대응표본 t-test를 사용하였다. 본 연구의 결과, 실천적 추론 가정과 수업은 중학생들의 자아존중감을 향상시키고 특히 자아존중감 하위영역 중 일반적 자아존중감과 사회적 자아존중감을 향상시키는 것으로 나타났다. 구체적으로 실천적 추론 수업은 학생들로 하여금 결심하면 실천하도록 하며, 자신에 대해서 만족하고, 혼자의 힘으로 문제를 해결하도록 할 뿐만 아니라 다른 사람이 자신을 좋아하고, 원하면 친구를 사귈 수 있다고 생각하게 하는데 효과적인 수업이라는 결론을 내린다.

미국 장기요양시설 내 의료 관련 감염 감시의 기준

채정미 건강보험심사평가원 2019 HIRA 정책동향 Vol.13 No.4

청소년 성 인식도 조사

채정미,김도형,김해숙,남기정,정영애,주지은,지대원,김오택,허재석 경산대학교 학생생활연구소 1996 학생생활연구 Vol.2 No.1

기능에서 신체분절의 협응과 기여

채정병,Chae, Jung-Byung 대한고유수용성신경근촉진법학회 2017 PNF and Movement Vol.15 No.1

Purpose: This study investigated the coordination and contribution of body segments during functioning. Methods: The relevant literature related to body segments and function were reviewed. Results: Efficient control of function is considered with regard to a participant's ability to perform a sequence of movements in body segments, which progresses from the head to the arm, trunk, pelvis, and leg segments. Each segment performs a specific role, which environment explorer using visual information for the head, reaching and grasping for the arms, a stabilizer for the trunk, and the distribution of COM in the pelvis and leg. Conclusion: During any of the movements, the momentum generated by the proximal segments is transferred to the adjacent distal segments in an appropriate sequence. In assessing function for clinical intervention strategies, the segment coordination, segment sequence, transfer of the center of body mass, asymmetrical ratio, muscle activity, and compensatory strategies should be considered.

인지상태와 연령에 따른 손 기능의 비교

채정병,한승협,Chae, Jung-Byung,Han, Seung-Hyup 대한고유수용성신경근촉진법학회 2014 PNF and Movement Vol.12 No.4

Purpose: The purpose of this study is to analyze the hand functions of elderly persons according to their cognitive status and age. Methods: A total of 65 persons voluntarily participated in the study. The subjects were divided into three groups: impairment cognitive group, normal cognitive group, adult group (persons in their twenties). Assessment of cognitive status was performed using a mini-mental state examination for Koreans (MMSE-K). Hand function was assessed using the Purdue pegboard test. The collected data were analyzed using a one-way ANOVA and Pearsonn Acorrelation. Results: There were significant differences in hand functions in the three groups. Post-hoc test results showed significant differences between each group. There were statistically significant differences in the correlation among hand functions, cognitive status, and age. The findings of this study suggest that hand functions have a positive correlation with cognitive status. However, a negative correlation was found between hand function and age. Conclusion: According to the study's results, hand functions are correlated with age and cognitive functions in elderly persons. This study suggests that hand rehabilitation with cognitive intervention increases hand functions in elderly persons.

Mycoplasma 성 왕쥐똥나무 빗자루병에 관한 연구

채정기(Jyung Ki Chai),김영호(Young Ho Kim) 한국산림과학회 1989 한국산림과학회지 Vol.78 No.2

The occurrence of witches` broom in Ligustrum ovalifolium was first noticed in Korea by author in L984. The present study was carried out with particular emphasis on the symptomatology, etiology, transmission of the disease and antibiotic treatments. The infected tissue was observed by the fluorescence and electron microscopy and its biochemical characteristics were compared with healthy one by electrophoresis. The results are summarized as follows : I, symptoms of the infected trees were characterized by the dwarfing of the organs, yellowing and brooming of the foliage. 2. The observation by the trans electron microscopy on the witches` broom of L. ovalifolium revealed the occurrence of numerous mycoplasma-like organisms(MLOs) in the phloem tissue cells of the midribs of infected leaves. 3. The MLOs were surrounded by a single unit membrane, and they appeared to be multiplied by binary fission. 4. The presence of crystals unidentified in the phloem parenchyma cells was noticed by electron rnicroscopy, 5. The disease was able to be transmitted by budding, crown, and greenwood graftings to L. ovalifolium, L. obtusifolium, L, japonicum and also transmitted, even when the stocks and scions were not completely grafted. 6. Insect transmission on L. ovalifolium and L, obtzrsifolium was carried by Hishiyrconus sellatus. 7. The infected roots dipped in the 1,000 ppm of teracyclin solution was only temporarily effective in controlling the disease. 8. Infected plant with MLOs showed specific fluorescent reactions in phloems with DAPI stain. 9. The protein and peroxidase separated by electrophoresis showed strikingly distinctive difference between the healthy and diseased leaves.

인지행동치료의 치료적 효능(efficacy)과 유효성(effectiveness)

채정호,김원,유태열 한국인지행동치료학회 2003 인지행동치료 Vol.3 No.1