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조서영,손유정,박세영,손진아,유지인,BARITUGOKEIANNE,Yokimiko David,강경희,김호용,최종일,이미나,김희택,주정찬,박시재 한국화학공학회 2021 Korean Journal of Chemical Engineering Vol.38 No.7
Sugarcane molasses was examined for the production of poly(3-hydroxybutyrate) [P(3HB)] and poly(3- hydroxybutyrate-co-lactate) [P(3HB-co-LA)] in recombinant Ralstonia eutropha strains expressing Mannheimia succiniciproducens sacC gene encoding -fructofuranosidase, which can hydrolyze sucrose into glucose and fructose in the culture medium. When crude sugarcane molasses was added to the culture medium to support 20 g/L of sucrose in flask cultivation, the growth of R. eutropha NCIMB11599 expressing the sacC gene was significantly inhibited, which resulted in OD600 of 1.2 with P(3HB) content of 0.1wt%. The inhibition of cell growth due to the usage of the crude sugarcane molasses was relieved by pretreatment of sugarcane molasses with activated charcoal. Sugarcane molasses pretreated with activated charcoal could support the growth of R. eutropha NCIMB11599 expressing the sacC gene to OD600 of 87.2 with P(3HB) content of 82.5 wt% in batch fermentation when it was added to culture medium to support 20 g/L of sucrose. Also, R. eutropha 437-540 expressing Escherichia coli ldhA gene encoding lactate dehydrogenase along with the sacC gene produced P(3HB-co-6.2mol%LA) with 29.1 wt% polymer content from sugarcane molasses in batch fermentation.
후기청소년의 자기주도학습이 진로적응성에 미치는 영향: 진로결정 자기효능감의 매개효과
조서영,강영배 한국청소년시설환경학회 2022 청소년시설환경 Vol.20 No.3
This article aims to verify the mediating effect of the self-efficacy of career decision in the relationship between self-directed learning and career adaptability of post-adolescents. The subjects of the study for analysis were 316 university students in Daegu and Gyeongbuk. The results of the study show. First, post-adolescents the self-directed learning and its sub-variables including learning motivation, learning strategy, self-awareness in order has a meaningful effect on the career adaptability. Second, post-adolescents the self-directed learning and its sub-variables including learning strategy, self-awareness, learning motivation in order has a meaningful effect on the self-efficacy of career decision. Third, post-adolescents the self-efficacy of career decisions and its sub-variables including goal selection, job information, future plans in order has a meaningful effect on the career adaptability. Fourth, the analysis result of the correlation between the self-directed learning and the self-efficacy of career decision shows that the self-directed learning and the career adaptability and the self-efficacy of career decision have a meaningful positive correlation with each other. And, the results of multiple regression analysis and Sobel Test show that the self-efficacy of career decision has a meaningful partial mediating effect in the relationship between the self-directed learning and the career adaptability.