SiO2/SiOx 다층구조 내 결함조절을 통한 실리콘 나노결정의 발광효율 최적화에 관한 연구

정지운 서울시립대학교 2016 국내석사

RF-magnetron sputtering system을 사용하여 다양한 조건으로 실리콘 산화막 다층구조 내 실리콘 나노결정을 제작한 후 시료제작 조건에 따른 발광현상에 관하여 연구하였다. 증착 시 기판의 온도를 조절하는 방법과 증착 후 양성자 조사를 하는 방법을 이용하여 다층구조 내 결함을 조절 함으로서 실리콘 나노결정의 형성을 증가시켜 PL (photoluminescence spectroscopy) 의 강도를 증대 시킬 수 있었다. 다층구조 내 결함이 PL Intensity증가에 끼치는 영향을 연구하기 위해서 흡수 분광법 및 PL측정을 통해 결함상태를 분석하였다. 또한 XPS (x-ray photoelectron spectroscopy) 측정을 통해 시료의 조성비 분석을 실시하였다. 다층구조 내 결함분석 결과 결함의 양에 따라서 생성되는 실리콘 나노결정의 양이 변화하는 것을 알 수 있었고 이는 실리콘 나노결정 형성 시 구조 내 결함이 산소의 확산에 핵심적 역할을 함으로써 나노결정 형성의 근원적 역할을 하는 것으로 이해되었다. We studied luminescence properties of Si-NC (silicon nanocrystal) embedded in silicon oxide multilayers deposited by RF-magnetron sputtering. Substrate temperature variation and proton irradiation were used to control various defect generation in the multilayers. PL (photoluminescence spectroscopy) intensity is increased by increasing formation of Si-NC. We performed absorption spectroscopy and PL measurements for study of the effect of the defect generation on the PL intensity variation. XPS (x-ray photoelectron spectroscopy) measurements are performed to characterize the change of the chemical states for all the relevant core-levels of elements in the samples. On the contrary to what the researchers expect, we found that the oxygen related defects formed in as-deposit SiOx layer by substrate temperature control and proton irradiation help to form very efficiently Si-NCs. We note that a main cause of improved Si-NC formation is attributed to the enhanced readiness of oxygen out-diffusion in the SiOx layer. By the method to control various defects in this study we can enhance readily luminescence efficiency for the silicon related nanostructures.

대학 품새선수의 유단자 품새 수행시 에너지소비 비교

정지운 경희대학교 2013 국내석사

The purpose of this study is to provide amount of taekwondo Poomsae for black belts in different two ways. First way was to analysis of each Poomsae performed once. Second means was conducted to examine in each Poomsae while 10 consecutively times. These two kinds of results will be offer to basic data of taekwondo training program which is composed of basic skills, target kicking, Poomsae and sparring. For this study, 12 males of elite Poomsae athletes of Kyung Hee university were involved and measured physiological responses. Measurement variables are performing time, pace, HR, VO2, Carbohydrate oxidation rate(COR) and Fat oxidation rate(FOR). Exercise intensity and energy expenditure were measured by VmaxST and Polar-system during poomsae performed. The statistical techniques for data analysis were applied descriptive statistics(mean ±SD), one-way repeated ANOVA on the SPSS 20.0, accepting level for all significance was above =0.05. On the basis of the results analyzed in this study, conclusions were drawn as follow; 1) Among 6 black belts Poomsae, Sipjin takes longest performance time as 85.2±4.4seconds, and Taebaek records fastest performance speed as 0.62motion/sec. Also, Pyongwon records highest kicking motion ratio of upper-lower body action as average of 11.5%, 2) During single performance, Goryo records highest heart rate scores as 128.9±9.1beat/min and VO2 as 26.3±2.8ml/kg/min. 3) Averagely, during 10 consecutive performance, the mean of HR was 179.5±20.1beat/min, and the mean of VO2 was 45.3±9.0ml/kg/min. 4) Averagely, during 10 consecutive performance, the mean of consumed carbohydrate was measured as 94.5±27.1g/h, and average quantity of consumed fat was 28.5±7.4g/h. In conclusion, people can train poomsae for their health by controlling repeated times or training times. 본 연구의 목적은 태권도 수련 프로그램의 주요 영역에 해당하는 품새를 대학 수준의 엘리트 품새 선수들이 유단자 품새 1회 수행과 10회 연속 수행 시 에너지소비 특성을 분석하고, 품새 개발과 품새 수련 프로그램 개발에 필요한 운동생리학적 기준을 제공하는데 있다. 본 연구의 대상자는 K 대학교에 재학 중인 남자 품새선수 12명으로, 이들은 하루 3시간씩 규칙적으로 품새 수련을 하고, 전국대회 규모에서 최소 1회 이상 1위를 한 경험이 있으며, 최근 6개월 동안 발목 및 허리에 정형외과적 부상이 없는 선수로 선발하였다. 본 연구의 주요 종속 변인은 품새 수행 시간, 품새 수행 속도, 심박수, 산소섭취량, 탄수화물 사용량, 지방 사용량이며 측정결과는 다음과 같다. 1) 유단자 품새 중 십진이 85.2±4.4초로 가장 길었으며, 태백이 0.62동작/초로 가장 빠른 속도를 나타냈다. 그리고 평원이 품새 동작에서 하지 동작비율이 11.5%로 가장 높았다. 2) 1회 수행 시, 고려가 심박수 128.9±9박/분, 산소섭취량 26.3±2.8ml/kg/min으로 가장 높은 산소섭취량을 나타냈다. 3) 10회 수행 시 평균 심박수는 179.5±20.1박/분이었으며, 평균 산소섭취량은 45.3±9.0ml/kg/min이었다. 4) 10회 수행 시 평균 탄수화물 사용량은 94.5±27.1g/h였고, 평균 지방 사용량은 28.5±7.4g/h이었다. 이상의 결과를 종합해 볼 때, 품새 수행은 중·고강도에 속하는 운동으로서 품새 수련자의 목적에 따라 수행회수와 시간을 적절하게 안배하여 건강을 목적으로 수련할 수 있는 운동이라고 사료된다.

공포학습 후의 편도체 불활성화가 조건공포와 능동회피반응에 미치는 영향

정지운 全南大學校 大學院 2004 국내석사

본 연구는 편도체 불활성화가 조건공포와 능동회피반응에 미치는 영향에 대해 알아보았다. 이를 위해 캐뉼라 이식수술을 하고 동물에게 능동회피훈련을 시킨 후 조건공포와 능동회피반응을 검사하기 직전에 약물주입을 하였다. 그 결과 muscimol 주입진단이 통제집단에 비해 활동성이 유의하게 증가되어 나타난 반면, 능동회피반응에서는 두 집단이 다르지 않았다. 즉 편도체 불활성화가 조건공포는 손상시키면서 능동회피반응은 손상시키지 않았다. 이 결과는 편도체의 역할에 대한 상반된 견해인 기억저장견해와 기억조절견해 모두가 부분적으로 옳으며, 두견해간의 불일치는 부분적으로 과제차이에 기인했을 가능성을 시사한다. 즉 편도체는 조건자극과 무조건자극의 연합이 일어나는 장소이면서, 동시에 운동기억이 편도체 이외의 장소에 응고화 되도록 조절하는 역할을 하며, 이는 조건공포와 능동회피반응을 매개하는 신경학적인 메커니즘이 일치하지 않을 가능성을 시사한다. This study examined the effects of the amygdala inactivation on conditioned fear and active avoidance. Rats, with chronic cannula placed bilaterally in the amygdala, were trained in an active avoidance task and 1 or 2 days later tested for both conditioned fear and active aviodance immediately afger muscimol or buffer injection. As a result, activity to the CS in the amygkala inactivation group was larger than that of controls while performance of active avoidance was not different. In other words, amygdala inactivation impaired conditioned fear but not active avoidance. Thes findings suggest that both memory storage and memeory modulation, different view about the role of amygdala, are correct in part and this discrepancy stem from the task difference in part. Thus, amygdala seems to be the locus of cs-us association and, at the same time, moduates memory consolidation in other brain regions. It means that neural mechanisms for the conditioned fear and active avoidance are not the same.

로마서 10장5-10절의 구약인용 연구

정지운 총신대학교 일반대학원 2013 국내석사

본 논문은 로마서 10장 5-10절에 나타난 레위기 18장 5절과 신명기 30장 12-14절의 인용의 해석학적인 의도와 의미를 밝히는데 목적을 두고 있다. 우선 제 1장에서는 서론으로서 본 논문의 문제제기 및 연구 방법에 대해서 간략히 소개하고, 전체 논문이 어떻게 진행되는지에 대한 연구 절차를 밝히고 있다. 제 2장에서는 신약의 구약 인용에 대한 다양한 해석학적인 입장을 살펴봄을 통해서 Enns의 Christotelic methodology(그리스도 완결적 방법론)이 신약 저자의 구약 인용에 있어서 가장 비문법적-비역사적 해석에 대한 실마리를 제공하는 해석학적 방법론임을 제시한다. 제 3장에서는 로마서 10장 5-10절의 선행 연구를 간략하게 살펴봄을 통해서 지금까지 로마서 10장 5-10절의 구약 인용에 해석에 대한 큰 그림을 그려보고 선행 연구들의 공헌은 무엇이며, 그들이 놓쳐 온 것은 무엇인지 설명한다. 제 4장에서는 MT(히브리성경)와 LXX(70인경)와 NT(신약성경) 본문을 분석해봄을 통해서 사도바울의 구약 인용의 실제적인 모습과 해석학적인 의도를 분석해본다. 제 5장에서는 로마서 10장 5-10절의 구약 인용 본문이 유대교 해석학과의 어떠한 관련성을 가지며 그것이 사도바울의 해석학과는 어떠한 차이를 가지는지에 대해서 살펴보고자 한다. 제 6장에서는 선행 연구를 통해서 얻은 로마서 10:5-10절의 신학적 의미에 대해서 정리해 봄을 통해서 본문을 통해서 전달하고 싶었던 사도바울의 의도를 파악하고자 한다. 마지막으로 제 7장에서는 지금까지의 논의를 총 정리하며 로마서 10장 5-10절의 구약 인용에 대한 본 연구 논문의 결론을 제시한다.

암환자에 대한 十全大補湯의 유효성 및 안전성: 체계적 문헌고찰 및 메타분석

정지운 경희대학교 대학원 2023 국내박사

Background and Objective: Sipjeondaebo-tang is one of the prescriptions used to improve the immunity of cancer patients and relieve cancer symptoms or side effects caused by cancer treatment. Therefore, in order to provide a higher level of evidence for using Sipjeondaebo-tang to cancer patients, we intend to systematically analyze the therapeutic effects and safety of Sipjeondaebo-tang for cancer patients. Methods: By August 29, 2022, a systematic review and meta-analysis were conducted through a screening process after searching for randomized controlled clinical studies in which Sipjeondaebo-tang was applied to adult cancer patients in 9 domestic and foreign databases. All the results reported in each individual study were summarized and meta-analysis was performed for studies with the same evaluation index, and qualitative analysis was performed for studies with different evaluation indexes. To evaluate the quality of individual studies included in this study, the risk assessment tool of bias in the Cochrane library was used. Results: 430 studies were retrieved from 9 databases. A total of 37 studies were finally included according to the selection and exclusion criteria. Among them, 30 studies were included in the meta-analysis. After surgery, Sipjeondaebo-tang significantly improved ALB, PALB, TP as a nutritional index, RBC, Hb, TRF as a hematological index, and immune index in cancer patients after surgery. Sipjeondaebo-tang did not show any significant improvement effect on AMC, TSF, and wound drainage as nutritional index of cancer patients after surgery. Sipjeondaebo-tang showed significant improvement in WBC, Hb reduction, vomiting, KPS, CD4+, CD8+, CD4+/CD8+ during chemotherapy in cancer patients. Sipjeondaebo-tang did not show any significant improvement effect on RECIST, PLT, abdominal pain, TFG-beta1, and CD3+ during chemotherapy for cancer patients. Sipjeondaebo-tang significantly improved CD3+, CD4+/CD8+ in cancer patients who were unable to undergo surgery, chemotherapy, and radiation therapy. Sipjeondaebo-tang did not show any significant improvement effect on CD4+ and CD8+ in cancer patients who were unable to undergo surgery, chemotherapy, and radiation therapy. Sipjeondaebo-tang significantly improved the fatigue of cancer patients, and there were no serious side effects from Sipjeondaebo-tang. Conclusion: Sipjeondaebo-tang significantly improved the nutritional and immune status of cancer patients. In addition, Sipjeondaebo-tang relieved cancer symptoms or side effects caused by chemotherapy, and there was no difference in adverse reactions compared to the control group.

비디오와 파워포인트를 활용한 아랍어 교수법 연구

정지운 朝鮮大學校 大學院 2004 국내석사

These days, as the Arab world has been a focus of world news, an interest in Arabic is increasing. Considering its position in religion, literature as well as politics and economy, and a considerable number of Arab language speakers, the education of the Arabic language and literature is important. Therefore, Arabic was designated as a subject of the second language in middle and high schools from the seventh curriculum. However, for the Arabic language and literature education to be more effective, teaching materials and methods must be improved to meet the needs of this generation. Of various teaching methods, for example, an audio-visual teaching program in multi-media is effective in provoking attention and interest from learners in image-focused generation. As learning is carried through video programs, it is easy for students to remember what they have learned from class. However, as video materials are designed only to present something to learn, the presentation of video should be accompanied by evaluation of students' language proficiency. Video programs present reliable language data through conversation. And, after the completion of video program, a variety of learning activities and emotional aspects of learners are stimulated so that understanding of the language can be enhanced. Multi-media allows teachers to be flexible with their instructions. The program such as power-point can provide dynamic presentation using audio-visual effect. For example, it can present reinforced clear and realistic colors and styles and adjust letters to have appropriate size and colors according to their specific importance. And various qualities of sound add liveliness to learning atmosphere. This study aims to present an effective teaching method to improve communicative ability of the Arabic by letting students repeat the conversation presented through video programs. For the purpose, the conversation in Arabic was presented to the students through video program consisting of lively conversation and culture, and to prevent the class from focusing only on the seeing and hearing of the video program, the students was promoted to repeat the conversation through presentation of power-point program. Unlike the simple teaching media of the past, a new teaching media called multi-media appeared due to the development of information technology. To adjust such changes, teachers should deviate from the conventional teaching method and create a better learning environment for students by taking advantage of multi-media. Multi-media have been effectively used in different areas and can be applied to education. This study will present an effective teaching method using power-point program, one of computer application programs. Arabic has had shorter history compared with that of other languages. Thus, teaching and learning materials for the education have not been developed enough to satisfy the needs of learners. However, the most important thing is to consider the effect of multi-media on the education of Arabic and continue to develop effective teaching methods and materials. Professional educators should explore multi-media teaching materials whose effects are still poor. Also, it is expected that more effective and various teaching methods will be developed through the teaching model presented in this study with a help of previous teaching methods and materials.

초등학교 저학년의 Body Action Therapy와 체력 및 주의집중의 관계

정지운 한국체육대학교 사회체육대학원 2021 국내석사

ABSTRACT The Relationship between physical strength and attention with body action therapy of lower grade elementary school students Jung Ji Woon Major in Lifetime Sport Graduate School of Sport and Leisure Studies This paper introduces the relations of Body Action Therapy to physical fitness and attention in the lower grades of elementary school. To investigate this, we conduct a study with 17 students in the first grade of elementary school, 9 of whom are in the experimental group and 8 in the control group. We perform Two-way ANOVA to compare the values, and if our result provides significant findings, we perform a t-test as a post-test. The study measures physical athletic performances by physical composition, health-related fitness, and skill-related strength and assesses attention duration by MR.CUCUI and number search test. We conducted the study eight times in four weeks. Each treatment took place twice a week, and the treatment time was about 30 minutes. In the 1st and 8th sessions, we measured pre and post-physical exercise ability and attentional concentration. During the 2 to 7 times sessions, the experimental group took physical education classes after applying Body Action Therapy, while the control group took regular physical education classes. In our study, we elicit four things as follows. First, we analyze the weight and BMI of the subjects, which is one of the components of body composition. The body weight increases, while BMI decrease in both two groups. But this is very slight, which does not show any significant differences. Based on the results, we found that Body Action Therapy had little relationship with the physical composition of elementary school students. We assume that a long-term study is necessary if we need to see the effect of body composition. Second, We explore cardiorespiratory endurance, muscle strength, and flexibility to check health-related fitness. Both groups showed an increase in cardiorespiratory endurance, but there was a significant difference. The experimental group improved more than the control group. The muscle strength decreased in both groups, and there was no significant result and no difference between the groups. Flexibility increased in both groups, but there were no meaningful results and no differences between groups. Based on the results, the study provides that Body Action Therapy had a small relationship with the health-related fitness of elementary school students. Third, We study agility, power, and coordination for skill-related fitness. Both groups showed an improvement in quickness. The research shows that the experimental group is quicker than the control group. The power increased in both groups, but there were no significant results and no differences between groups. Coordination ability increased in both the experimental group and the post-test group. The results of coordination show a noticeable difference between them. According to the results, Body Action Therapy had a relationship with the skill-related fitness of elementary school students. Fourth, as a result of measuring attention span by MR.CUCUI and Number Search Test, both MR.CUCUI and Number Search Test improved in both groups. Also, there was no significant result and no difference between them. Considering the research, We cannot find any relevance between Body Action Therapy and elementary school students' attention time. All the data shows the opposite result compared with previous studies. We infer why the outcome is different from the earlier works. It is because the study period was shorter than the prior studies. Also, the intensity of Body Action Therapy movements was lower than those works. So, to observe the overall development through Body Action Therapy, the subject should need the capacity to perform medium and high-intensity motions over a long period. We take this as evidence that Body Action Therapy is an exercise suitable for upper elementary school students, middle school students, and high school students who have the capability. 국문초록 초등학교 저학년의 Body Action Therapy와 체력 및 주의집중의 관계 정 지 운 한국체육대학교 사회체육대학원 생활체육 전공 본 연구는 초등학교 저학년의 Body Action Therapy와 체력 및 주의집중의 관계를 조사하기 위하여 초등학교 1학년 학생 실험집단 9명 통제집단 8명 총 17명을 대상으로 신체 운동능력 측정과 주의집중력의 사전, 사후 측정값을 비교하는 이원 변량 분석(Two-way ANOVA)을 실시하였고 유의한 결과가 나타나면 사후 검증으로 t-test를 실시하였다. 신체 운동능력은 신체구성, 건강체력, 운동체력 3가지의 측정값으로 연구하였고 주의집중력은 MR.CUCUI, 숫자찾기 테스트의 측정값으로 연구하였다. 연구는 주 2회 4주간 총 8회, 1회 처치 시간은 30분 내외로 처치하였고 1회기와 8회기는 사전, 사후 신체 운동능력 측정과 주의집중력 검사가 실시되었고 실험집단은 2회부터 7회까지 Body Action Therapy 적용 후 체육수업을 진행하고, 통제집단은 일반 체육수업을 진행하였다. 자료 분석을 통한 연구의 결과는 다음과 같다. 첫째, 신체구성 중 체중, BMI를 연구한 결과 체중은 두 집단이 소폭 상승하였고, BMI는 두 집단이 소폭 하락하였지만 유의한 결과가 나타나지 않았고 집단 간 차이도 나타나지 않았다. 체중과 BMI 연구결과로 보아 Body Action Therapy가 초등학생들의 신체 구성과 큰 관계가 없는 것으로 나타났다. 신체구성의 효과를 보기 위해서는 단기간이 아닌 장기간의 연구가 필요하다고 사료된다. 둘째, 건강체력 심폐지구력, 근력, 유연성을 연구한 결과 심폐지구력은 두 집단이 상승하였고 유의한 결과와 집단 간 차이가 나타났으며, 실험집단이 통제집단에 비하여 높게 향상되었다. 근력은 두 집단이 하락하였으며, 유의한 결과가 나타나지 않았고 집단 간 차이도 나타나지 않았다. 유연성은 두 집단이 상승하였지만 유의한 결과와 집단 간 차이가 나타나지 않았다. 심폐 지구력에서 나타난 유의한 결과와 집단 간 차이의 연구 결과와 근력, 유연성 연구 결과로 보아 Body Action Therapy가 초등학생들의 건강체력과 작은 관계가 있는 것으로 나타났다. 셋째, 운동체력 민첩성, 순발력, 협응력을 연구한 결과 민첩성은 두 집단이 상승하였고 유의한 결과와 집단 간 차이가 나타났으며, 실험집단이 통제집단에 비해 높게 향상되었다. 순발력은 두 집단이 상승하였지만 유의한 결과와 집단 간 차이가 나타나지 않았다. 협응력은 두 집단이 상승하였고 유의한 결과가 나타나지 않았지만 집단 간 차이에서 실험집단이 사전보다 사후에 유의한 차이가 나타났다. 민첩성과 협응력에서 나타난 유의한 결과와 집단 간 차이의 연구결과와 순발력의 연구결과로 보아 Body Action Therapy가 초등학생들의 운동체력과 관계가 있는 것으로 나타났다. 넷째, 주의집중력 MR.CUCUI, 숫자 찾기를 연구한 결과 MR.CUCUI는 두 집단이 상승하였지만 유의한 결과와 집단 간 차이가 나타나지 않았다. 숫자 찾기는 두 집단이 향상되었지만 유의한 결과와 집단 간 차이가 나타나지 않았다. MR.CUCUI, 숫자 찾기 테스트 연구결과로 보아 Body Action Therapy가 초등학생들의 주의집중력과 큰 관계가 없는 것으로 나타났다. 선행연구와 본 연구의 결과가 다른 이유는 연구기간을 단기간으로 진행한 것과 Body Action Therapy 동작의 강도를 저강도로 진행한 것에 있다고 사료된다. Body Action Therapy를 통하여 전체적으로 발달 된 효과를 보이기 위해서는 중, 고강도의 동작을 장기간 할 수 있는 초등학교 고학년, 중학생, 고등학생 등 더 높은 연령에 적합한 운동이라는 것을 알 수 있었다.

녹차씨앗 추출물의 신생혈관형성억제 작용에 의한 항비만 효과

정지운 全南大學校 2009 국내석사

녹차 씨앗 추출물의 Anti-angiogenesis 메커니즘 조사를 위해 녹차씨앗추출물의 양을 달리하여 실시한 western blot에서는 신호분자들인 VEGFR-2, β-catenin, PI3-Kinase의 신호가 GSE에 의하여 차단되어 NF-κB까지 신호전달이 이루어지지 않음으로써, NF-κB가 활성화되지 못하여 신생혈관형성이 일어날 수 없다고 판단되었다. 또한 각각의 극성별로 hexhan, chloroform, ethlyacetate, butanol 및 물 층으로 분액 하여 각각의 분액에 대하여 신생혈관 형성억제 실험을 하여 butanol층에서 그 효과가 가장 좋음을 알게 되었다. 그리고 이 추출물을 이용 비만세포 3T3-L1의 비만 억제작용과 nude mouse에 이식하여 그 조직이 줄어드는 것과 혈액분석을 통해 중성지방의 형성을 억제하는 것을 알 수 있었다. 신호전달의 체계를 규명하여 신생혈관 형성억제작용에 따라 비만세포를 억제하여 체중의 감소가 일어나는 것을 확인하였다. 또한, S.D 계열의 쥐에 15일간 고지방식이 후 녹차 씨앗 추출물을 15일간 투여하였을 때 20%의 체중감소 효과를 나타내었다. 이러한 결과들로 볼 때 녹차씨앗추출물은 천연산물의 추출물로 부작용이 적다는 장점과 신생혈관형성과 직접 비만세포의 성장과 부착을 억제함으로써 항비만 효과를 나타내기 때문에 유용한 물질로 사료된다. For investigating the mechanism of anti-angiogenesis effect of greentea seed extract(GSE), western blot was performed. The pathways from the signal molecules of VEGFR-2, β-catenin and PI3-kinase to NF-κB in nuclear were interrupted with a dose-dependent concentration of GSE, therefore, angiogenesis couldn't be occured due to inactivation of NF-κB. And GSE was fractionated with hexan, chloroform, ethylacetate, butanol and water, butanol fraction was showed more powerful anti-angionegic effect than that of these fractions. The growth of mast cell-3T3-L1 was suppressed with GSE, and weight loss effect was showed by 20% with S.D rat which raised 15 days and treated with GSE for 15 days. Grafts implanted into nude mouse were shrinked, and triglyceride from blood analysis was also decreased by injected GSE. As GSE was showed lower toxicities, higher anti-angiogenic effect, decreasing weight gain, shrinking implanted mast cell grafts and reducing triglyceride from blood, it may be expected very useful anti-obesity materials from natural products with less side effect.

보라성게(Anthocidaris crassispina)의 알과 껍질 추출물의 항균활성 및 항산화 효과와 껍질의 열수추출물이 넙치의 비특이적 면역반응에 미치는 영향

정지운 제주대학교 대학원 2015 국내석사

Side effects of synthetic antibiotics and antioxidants, natural antibacterial substances and antioxidants present in natural products, and their human impact are the major research topics conducted to date. Recently, a study on natural antibacterial substances and antioxidants are concentrated in bio-active compounds of marine source has produced a unique metabolism and specificity. Sea-urchin called as “haedam” in oriental medicine, known for its excellent physiological activity. In this study, we use purple sea-urchin (Anthocidaris crassispina)'s shell and egg, was used to measured antimicrobial activity against human and fish bacterial pathogens, and its antioxidant activity. Also, we investigate effect of hot water extract of sea-urchin shell in the immune response of olive flounder and to check it's usage as feed additives. Hence for the study, 8.3955 g of A. crassispina egg hot water extract, 2.009 g of A. crassispina shell hot water extract, 3.1344 g of A. crassispina egg hexane layer, 0.3199 g of A. crassispina egg methylene chloride (CH2Cl2) layer, 0.2998 g of A. crassispina egg ethyl acetate layer, 2.5713 g of A. crassispina egg aqueous layer, 0.517 g of A. crassispina shell hexane layer, 0.0085g of A. crassispina shell CH2Cl2 layer, 0.0153 g of A. crassispina shell ethyl acetate layer and 0.8838 g of A. crassispina shell aqueous layer were extracted. Antibacterial activity of A. crassispina egg extracts were compared with that of A. crassispina shell extracts, which reveals that egg extracts were more effect than shell extracts. In DPPH radical scavenging activity, both egg and shell extracts show similar reducing activity but higher than that of control BHA and BHT. Especially, aqueous layer of egg extracts, hot water extract and ethyl acetate layer of shell’s antioxidant activities were excellent in low concentration. In hydroxyl radical scavenging activity, also show similar results with DPPH radical scavenging assay. However, hydroxyl radical scavenging activity of egg extracts is slightly higher than that of shell extracts. As a result in in vivo test, the hot water extract of A. crassispina shell was showed that do not effect on the non-specific immune responses of the olive flounder. The reason for this result, the feed uptake of olive flounder is presumably due to low during the experiment period. Further studies are need to detailed analyses of sea-urchin shell extracts and to encouraging the isolating biologically more potential substance.

남북 경제협력에 관한 연구 : 경제협력 사례를 중심으로

정지운 국방대학원 2002 국내석사