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두경부 유리피판 수술에 있어서의 비천공성 혈관 클립을 이용한 미세혈관 문합술
장학,민경원,김우람,신현우,고경석,Chang, Hak,Minn, Kyung-Won,Kim, Woo-Ram,Shin, Hyun-Woo,Koh, Kyung-Suck 대한미세수술학회 2005 Archives of reconstructive microsurgery Vol.14 No.1
Microvascular anastomosis with suture technique is a basic skill but there are several problems such as stenosis, thrombosis and long operating time. Recently plastic surgeons have developed non-suturing mechanical coupling devices for microvascular anastomosis. The authors applied non-penetrating vascular clips (VCS clips) in the field of free flap surgery of head and neck area. Between August of 2004 and January of 2005, we performed 9 free flaps (16 vessels) using small-sized VCS clips. Four stay sutures were applied first and then VCS clips were placed between sutures about 1 mm apart. Vascular pedicle of free flap included the descending branch of lateral circumflex femoral vessel, thoracodorsal vessel, deep inferior epigastric vessel and cephalic vein. The recipient vessels were the superior thyroid artery, superficial temporal artery, internal jugular vein, external jugular vein, and superficial temporal vein. We performed 13 end-to-end (4 arteries and 9 veins) and 3 venous end-to-side anastomoses. No flap related complication occurred but we applied additional clips or sutures in two cases due to blood leakage after completion of anastomosis. Primary patency rates seemed to be good and more rapid anastomosis could be done than conventional suture technique. Advantages of VCS technique are high patency rate, low thrombogenecity and rapidity. Although the high cost of VCS instrument may be a problem, this clip could be applied safely in microvascular free tissue transfer.
지방유래줄기세포의 지방분화과정에서 활성산소가 미치는 영향
장학,민경희,박영인,김요한,민경원 대한성형외과학회 2011 Archives of Plastic Surgery Vol.38 No.2
Purpose: Stem cells continue to receive research attention in the clinical fields, and adipose - derived stem cells (ADSCs) have been shown to be a good source raw material. Many plastic surgeons are researching the ADSC adipogenesis with a view to conducting clinical trials, and many attempts have been made to identify the factors that promote the adipogenesis of ADSCs, but comparatively few correlation studies have been undertaken to explore the relation between reactive oxygen species (ROS) and the ADSC adipogenesis. We undertook this study is to investigate the effects of ROS on ADSC adipogenesis. Methods: ADSCs were isolated and cultured from abdominal adipose tissue, and cultured in different media; 1) DMEM (control), 2) adipogenesis induction culture medium, 3) adipogenesis induction culture medium with ROS (20 μM / 50 μM H2O2), 4) adipogenesis induction culture medium containing ROS (20 μM / 50 μM H2O2) and antioxidant (10 μM / 20 μM Deferoxamine). We compared adipogenesis in these different media by taking absorbance measurements after Oil - Red O staining every 5 days. Results: After culturing for 20 days, significant differences were observed between these various culture groups. Absorbance results showed significantly more adipogenesis had occurred in media containing adipogenesis induction culture medium and H2O2(in a H2O2 dose - dependently manner) than in media containing adipogenesis induction culture medium and no H2O2 (p < 0.001). Furthermore, in media containing adipogenesis induction culture medium, H2O2, and antioxidant, absorbance results were significantly lower than in adipogenesis induction culture medium and H2O2 (p < 0.001). Conclusions: These findings suggest that ROS promote the adipogenesis of ADSCs. We suggests that ROS could be used in the adipose tissue engineering to improve fat cell differentiation and implantable fat tissue organization.
장학길,한명규,유량자 한국콩연구회 1988 韓國콩硏究會誌 Vol.5 No.1
大豆 및 大豆粉 저장중 단백질 특성에 미치는 영향을 經時的으로 검토하였다. 大豆 및 大豆粉의 저장중 수분흡수력은 수분함량이 증가됨으로써 감소되었다. 저장중 pH의 변화는 65% RH 에서는 大豆粉의 경우 pH 6이상 유지되었으나 85% RH에서는 pH 5.2까지 급격히 저하되었다. 大豆거장중 전질소 및 수용성 질소는 저장기간에 따라 계속 감소되었으며, 大豆粉을 85% RH에서 無包裝 상태로 저장하였을 때 수용성 질소는 70%이상 감소되었다. 저장중 HDPE 또는 OPP/Al/PE film을 사용함으로써 수분 및 pH의 변화가 감소되었으며. 전질소와 수용성 질소의 변화도 감소되었다. 저장중 大豆澱粉의 Amylograph 점도와 호화온도는 저장습도 및 포장재료에 따라 큰 차이가 있었다. 저장중 大豆 및 大豆粉 추출물의 calcium sulfate에 의한 응고력은 저장형태(大豆 또는 大豆粉), 저장기간, 습도 및 포장재료에 큰 차이가 있었으며, 특히 大豆粉은 無包裝 상태에서 90일 저장시 응고가 되지 않았다.