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한국산 고등 균류의 성분 및 배양에 관한 연구(제55보) : Lyophyllum decastes의 항암 성분 및 배양
이정옥(Chong Ock Lee),김형수(Hyung Soo Kim),최응칠(Eung Chil Choi),김병각(Byong Kak Kim) 한국생약학회 1986 생약학회지 Vol.17 No.1
To find antitumor components of Lyophyllum decastes, the mycelia of L. decastes were cultured in artificial media and a new antitumor component which showed potent antitumor activity against sarcoma 180 implanted in mice, was isolated and named Lyophyllan A. Lyophyllan A was composed of a polysaccharide moiety (86%) and a protein moiety (2%). The polysaccharide moiety was found to be a heteroglycan which consisted of glucose (48.1%), rnannose (30.8%), galactose, xylose and fucose. The protein moiety contained 18 amino acids. Cultural characteristics of L. decastes were investigated by shake culture method. The best result was obtained when L. decastes was cultured in medium glucose 50 g, peptone 10 g, corn steep liquor 30㎖, KH₂PO₄ 0.87 g, MgSO₄·7H₂O 0.5 g, CaCl₂ 0.3 g, FeSO₄·7H₂O 10 ㎎, MnC1₂·4H₂O 7 ㎎, ZnSO₄·7H₂O 4 ㎎ and CuSO₄·5H₂O 1㎎ per one liter at 26℃, 180 rpm, for 9 days.
몇 가지 전이금속, Ni(II), Cu(II) 및 Zn(II) 3,6-bis(2'-pyridyl)pyridazine 착 화합물들의 세포 독성효과
권병목,이정옥,최상운,성낙도,Kwon, Byung-Mok,Lee, Chong-Ock,Choi, Sang-Un,Sung, Nack-Do 한국응용생명화학회 2006 Applied Biological Chemistry (Appl Biol Chem) Vol.49 No.1
6종의 인체 암(폐암, 피부암, 결장암, 자궁암, 선암 및 뇌암)과 그의 17가지 세포주들에 대한 리간드 화합물 3,6-bis(2'-pyridyl)pyridazine(1) 과 3,6-bi s(6'-methyl-2'-pyridyl)pyridazine(2) 그리고 그들의 전이금속(Ni(II), Cu(II) 및 Zn(II)) 착 화합물들 $(3{\sim}6)$ 세포독성을 각각 측정하였다. 그 결과, 특히 Cu(II) 착 화합물, bis-[3,6-bis-(6'-methyl-2'-pyridyl)pyridazine-$k^2N^2,N^3$]chlorocopper(II)perchlorate (4)는 뇌암(SNB-19)과 결장암(SW-62) 세포주에 대하여 제1세대 항암제인 Cis-platin보다 높은 세포독성을 나타내었다. A series of cytotoxic activities $(ED_{50})$ in vitro against six human cancers (lung cancer, uterine cancer, skin cancer, brain cancer, colon cancer and adenocarcinoma) and their seventeen cell lines of 3,6-bis(2'-pyridyl)pyridazine, 1, 3,6-bis-(6'-methyl-2'-pyridyl)pyridazine, 2 and their transition metal, Ni(II), Cu(II) and Zn(II) complexes, $3{\sim}6$ were measured. Particularly, the results revealed that the cytotoxic activities against the brain cancer cell line (SNB-19) and the colon cancer cell line (SW62) of bis- [3,6-bis-(6'-methyl-2'-pyridyl)pyridazine-$k^2N^2,N^3$]chlorocopper(II)perchlorate, 4 were shown to be higher than that of the first generation anticancer agent, Cis-platin.
Myxococcus stipitatus JW150이 생산하는 세포독성 물질의 분리 및 특성
안종웅(Jong Woong Ahn),이정옥(Chong Ock Lee) 대한약학회 2002 약학회지 Vol.46 No.2
Drug resistance is one of the most significant impediments to successful chemotherapy of cancer. Multidrug-resistance (MDR) is characterized by decreased cellular sensitivity to anticancer agents due to the overexpression of P-gly-coprotein. By employing a resistant subline of HCT15 to adriamycin (CLO2), we undertook the screening for agents which were effective to multidrug-resistant cancer cells. As a result, a myxobacterial strain n7150 was selected for study since an activity against CLO2 cells was discovered in the strain. Cytotoxicity-guided of actionation of the culture broth led to the isolation of cystothiazole A and melithlazole F. The producing organism was identified as Myxococcus stipitatus by taxonomic comparison with type strains of Myxococcus sp. As well as its morphological and physiological characteristics. Cystothiazole A and melithiazole F demonstrated potent cytotoxicity against certain human cancer cells with IC50 values ranging from 0.03∼0.72μg/ml. Both compounds were interestingly as active against drug- resistant sublines CLO2 and CP7O as against the corresponding parental cells.
박성희,조성지,이인선,이정옥,김종원,이인자,Park, Sung-Hee,Cho, Sung-Ji,Rhee, In-Sun,Lee, Chong-Ock,Kim, Chong-Won,Rhee, In-Ja 한국생약학회 1996 생약학회지 Vol.27 No.4
The objective of this reseach is to find new antitumoral substances from natural products. Several of natural products have been used as food that were isolated into hexane(Hex.) and/or ethylacetate(EtOAc) extracts. we have tested cytotoxicities of these plants against human solid tumor cells. The cytotoxic activity of these plants were tested using Sulforhodamin B(SRB) assay. Hexane extracts of Chrysanthemum sinense, Allium tubersum. Beta vulgaris, Ixeris dentata have revealed cytotoxicities against five human solid tumor cells, and its cytotoxicities of each cell line were $10-100\;{\mu}l/ml$ ED50 (Effective dose that cause 50% inhibition of cell growth in vitro)
한국산 고등균류의 성분 연구 (제42보) : 이끼살이애기버섯 및 검정나팔버섯의 항암성분
이경림(Kyung Lim Lee),김혜령(Hye Ryung Kim),이정옥(Chong Ock Lee),정경수(Kyung Soo Chung),최응칠(Eung Chil Choi),김병각(Byong Kak Kim) 한국생약학회 1982 생약학회지 Vol.13 No.4
The carpophores of Xeromphalina campanella and Craterellus cornucopioides which were collected at the Gwang-neung area, Gyeong-Gi Province, in 1981 were studied for their antitumor constituents. The carpophores were extracted with not water and alconol precipitates were obtained. These precipitates were brownish powder which were used as samples in antitumor test. The antitumor activity of each sample was tested against sarcoma 180 implanted subcutaueously in ICR mice. The sample obtained from the carpophores of X. campanella showed the inhibition ratios of 47% and 58% at the dose levels of 20㎎ and 50㎎/㎏/day, respectively. The sample obtained from the carpophores of C. cornucopioides showed no activity. The chemical analysis of the antitumor fraction of X. campanella showed that it contained a polysaccharide. The hydrolysates of the polysaccharide moieties contained five monosaccharides: glucose, galactose, mannose, fucose and xylose.
이승호(Seung Ho Lee),유시용(Shi Yong ryu),최상운(sang Un Choi),노재성(Zaesung No),김성기(Sung Ki Kim),이정옥(Chong Ock Lee),안종웅(Jong Woong Ahn) 한국생약학회 1995 생약학회지 Vol.26 No.1
EtOAc soluble part of MeOH extract of Anemarrhena asphodedoides rhizome was evaluated for the cytotoxicity against the five kinds of human tumor cell lines (A-549, SK-OV-3, SK-MEL-2, XF498 and HCT15) in vitro. Bioassay-guided fractionation of EtOAc soluble part led to the isolation of active compound which was identified as timosaponin A-III showed potent cytotoxic activity, but its genin, sarsasapogenin, did not show cell growth inhibition.
이형규(Hyeong Kyu Lee),변순정(Soon Jung Byon),오세량(Se Ryang Oh),김정일(Jung Il Kim),김영호(Young Ho Kim),이정옥(Chong Ock Lee) 한국생약학회 1995 생약학회지 Vol.25 No.4
A new diterpene, agastanol[l] with dehydroagastol[2] was isolated from the root of Agastache rugosa, and their structures were elucidated by chemical and instrumental analysis. Agastanol[1], its derivatives, agastanone[3] and methylagastanol [5], and dehydroagastol[2] showed cytotoxic activities against in vitro human cancer cell lines. Agastanol[1] showed weak antifungal activity against Trichophyton rubrum.
새로운 5-치환 Uracil 유도체의 합성 및 생물활성
이원희(Won Hui Yi),박정섭(Jung Sup Park),원정희(Jeong Hee Won),이정옥(Chong Ock Lee),유응걸(Eung K. Ryu) 대한약학회 1991 약학회지 Vol.35 No.6
Six novel 5-substituted-1-[2-(3-methoxy-2-hydroxyphenyl)-l-methoxyethyl]uracils 2a-f were prepared by condensation of 2,4-bis(trimethylsilyloxy)-5-substituted uracils with 2,7-dimethoxy-2,3-dihydrobenzofuran (9) in the presence of Lewis acid. The 2,3-dihydrobenzofuran derivative 9 was obtained by intramolecular acetalization of 2-acetoxy-3-methoxyphenyl acetaldehyde (8) which was synthesized by oxidative cleavage of 1-allyl-2-acetoxy-3-methoxybenzene (7) using osmium tetroxide followed by NaIO4. Compounds 2a-f were evaluated for in vitro antiviral activity against HSV-1, HSV-2 and HRV. None of these compounds showed activity with ID50) values up to 100 mcg/ml except for 5-chlorouracil derivative 2d which exhibited antiviral activity against HSV-1 with ED50) 30 mcg/ml. In the antitumor activity against L1210 and P388 leukemia cell lines, 2d showed activity with ID50 values of 14 mcg/ml and 11.6 mcg/ml, and 2c with ID50 values of 22.9 mcg/ml and 8.8 mcg/ml, respectively.
신나무로부터 분리한 methyl gallate의 세포독성연구
한두석(Du Seok Han),한성수(Seong Soo Han),이정옥(Chong Ock Lee),임요섭(Yo Sup Rim) 한국독성학회 1999 Toxicological Research Vol.15 No.3
In order to develop antitumor agent which has weak side effects and strong antitumor activcytotoxicity and antitumor effects of methyl gallate (MG) was evaluated by MTT and SRB assay of colorlmetric methods on the cultured NIH 3T3 fibroblasts and human oral epitheloid carcinoma cells (KB cells). NIH 3T3 fibroblasts were cultured in EMEM and KB cells were cultured in RPMI 1640 media. After incubation for 24 hrs, the cells were treated with MG by dose dependent manner (1, 25, 50, 100 μM), incubation 48 hrs, MTT and SRB quantity was measured by ELISA reader (Spectra Max 250, USA). The morphological changes of KB cells were observed by inverted microscope. IC50 by SRB assay of human lung tumor cells, human ovarian tumor cells, human melanoma tumor cells, human colon tumor cells and human CNS tumor cells, when MG was treated at them, was 69.80 μM, 44.90 μM, 22.12 μM, 29.24 μM and 59.29 μM, respectively. IC50 by SRB assay was 8701.23 μM in NIH 3T3 cells and 168.81 μM in KB cells treated with MG. Morphological changes of NIH 3T3 cells treated with MG were weak, but those of KB cells treated with MG were severe.