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이산화납/탄소 반죽 전극을 이용한 과당 농도 측정 스트립센서
이재선,조주영,허민,임우진,이상은,남학현,차근식,신재호,Lee, Jae Seon,Cho, Joo Young,Heo, Min,Lim, Woo-Jin,Lee, Sang Eun,Nam, Hakhyun,Cha, Geun Sig,Shin, Jae Ho 한국전기화학회 2014 한국전기화학회지 Vol.17 No.2
본 연구에서는 스크린 프린팅 방법을 이용하여 이산화납($PbO_2$)/탄소 반죽 전극을 제작하고, 이를 전기화학 방법의 과일 천연당(포도당, 자당, 과당) 측정용 센서로 이용하였다. 이산화납/탄소 반죽전극은 탄수화물과 같은 유기화합물의 전기화학적 산화촉매 신호를 측정함으로써 효소를 사용하지 않고도 당의 측정이 가능하다. 또한 측정 시 심각한 방해작용을 하는 아스코르브산(ascorbic acid)은 Nafion 막을 전극표면에 도입함으로써 효과적으로 감소시켰다. 최적화된 Nafion/이산화납/탄소 반죽 전극은 사람이 느끼는 상대당도(과당>자당>포도당)와 유사하게 각 당에 대한 감응신호를 나타내었다. A strip sensor based on $PbO_2$/carbon paste electrode was prepared by a screen-printing method, and employed to electrochemically determine the concentration of fruit sweeteners(i.e. glucose, sucrose, and fructose). The $PbO_2$/carbon paste electrode could monitor electrocatalytic oxidation of organic compounds such as carbohydrates, and measure the levels of natural sweeteners without enzyme. Severe interference from ascorbic acid was effectively reduced by modifying the electrode surface with a Nafion membrane. The response level of the Nafion/$PbO_2$/carbon paste electrode increased in the order of fructose, sucrose, and glucose, which corresponds to the order of sweetness perceived by humans.
임분 (林分) 대표치 측정을 위한 플롯크기에 관한 연구 (Ⅱ) - 6 년생 잣나무 인공림을 대상으로 -
이재선,한상섭 ( Jae Seon Lee,Sang Sup Han ) 한국산림과학회 1984 한국산림과학회지 Vol.65 No.1
The analysis of data about the 6-year-old Korean pine plantation was done in order to decide the suitable plot size for the survey on several stand characters. The plot size of at least 0.04 ha was recommendable for the investigation of height; but the size of 0.08㏊ for D.B.H. and for crown width.
콜라겐 유도 관절염에서 콜라겐 항원 특이 $V{\beta}3$+CD4+T 세포의 선택적 증식
이재선,조미라,이정은,민소연,윤종현,김완욱,민준기,박성환,김호연,Lee, Jae-Seon,Cho, Mi-La,Lee, Jung-Eun,Min, So-Youn,Yoon, Chong-Hyeon,Kim, Wan-Uk,Min, Jun-Ki,Park, Sung-Hwan,Kim, Ho-Youn 대한면역학회 2005 Immune Network Vol.5 No.2
Background: Collagen-induced arthritis (CIA) in mice is animal model of autoimmune disease known as rheumatic arthritis in human. We investigated CII-specific CD4+ T cell receptor usage in CIA mice. Methods: In CIA model, draining lymph node (dLN) CD4+ T cells and splenocytes at $3^{rd},\;5^{th},\;8^{th}$ week, we investigated CII-specific T cell proliferation, production of IL-17, IFN-${\gamma}$, TNF-${\alpha}$, IL-4 and IL-10. And we also performed anti-CII IgG Ab measurements in serum level, TCRV ${\beta}$ usage and T cell clonality with RT-PCR-SSCP analysis. Also, we performed proliferative response against CII when CII-specific T cell subset is deleted. Results: CIA mice showed more increase in the serum level of anti-CII IgG than normal mice after induction of arthritis. And the level of anti-CII IgG2a in CIA mice was increased after $3^{rd}$ week after primary immunization, while anti-CII IgG1 was decreased. Draining LN CD4+ T cells have proliferated against CII stimulation at $3^{rd}$ week after $1^{st}$immunization. CD4+T cells derived from dLN of CIA mice produced proinflammatory cytokine IFN-${\gamma}$, IL-17 etc. Draining LN CD4 T cells of CIA presented higher proportion of CD4+V ${\beta}3$+subset compared to those of normal mice at $3^{rd}$ week after $1^{st}$ immunization, and they were increased in proportion by CII stimulation. Draining LN CD4+ T cells without TCRV ${\beta}3+/V{\beta}8.1/8.2+/V{\beta}$10b+cells were not responsive against CII stimulation. But, CII-reactive response of TCRV ${\beta}3-/V{\beta}8.1/8.2-/V{\beta}$10b- T cells was recovered when $V{\beta}3+$ T cells were added in culture. Conclusion: Our results indicate that CD4+$V{\beta}3+$ T cells are selectively expanded in dLN of CIA mice, and their recovery upon CII re-stimulation in vitro, as well as the production Th1-type cytokines, may play pivotal role in CIA pathogenesis.
임분(林分) 대표치(代表値) 측정(測定)을 위한 플롯크기에 관(關)한 연구(硏究)(II) -6 년생(年生) 잣나무 인공림(人工林)을 대상(對象)으로-
이재선,한상섭,Lee, Jae Seon,Han, Sang Sup Korean Society of Forest Science 1984 한국산림과학회지 Vol.65 No.1
6 년생(年生) 잣나무 조림지(造林地)에 있어서 임분(林分)의 대표치(代表値) 측정(測定)을 위한 적정(適定) 플롯의 크기를 조사분석(調査分析)하였는 바, 수고(樹高)에 대하여는 0.04ha 크기의 플롯이, 흉고직경(胸高直徑)에 대하여는 0.08ha 크기의 플롯이 적합하였다. The analysis of data about the 6-year-old Korean pine plantation was done in order to decide the suitable plot size for the survey on several stand characters. The plot size of at least 0.04 ha was recommendable for the investigation of height; but the size of 0.08 ha for D.B.H. and for crown width.