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자립준비청년의 정서적 치유를 위한 자연친화적인 공간디자인 제안
이예린(Lee, Ye-Rin),윤주영(Yoon, Joo-Young),김유선(Kim, Yoo-Sun) 더나은도시디자인포럼 2023 도시디자인저널 Vol.5 No.2
Analysis of the status of young people preparing for independence revealed that psychological d a landscaping space using plants, which is an eco-friendly space to create a healing environment. This study targeted Hwayang-dong, Gwangjin-gu, the area with the highest proportion of single-person households in ousing, where nature and people meet and community living is possible in a common space, was set up in the center of the site to provide a sense of stability. We planned a space with the concept (title) N℃ (Neo-℃). We hope that the landscaping space for a healing environment will be one that can contribute to healing the minds of young people preparing for independence and helping them become self-reliant. Additionally, research and planning of spatial design for the socially disadvantaged should continue.
CRISPR/Cas9 시스템을 이용한 세포 사멸 불활성화(immortalization) 배추
이예린(Ye-Rin Lee),이혜은(Hye-Eun Lee),한고은(Koeun Han),이은수(Eun Su Lee),고기성(Ki Seong Ko),이균오(Kyun Oh Lee),김도선(Do-Sun Kim) 한국원예학회 2021 한국원예학회 학술발표요지 Vol.2021 No.10
배추(Brassica rapa)는 국내뿐만 아니라 중국 및 다른 동아시아에서 중요한 채소이다. 배추의 경우 애기장대가 속하는 배추과 식물로서 애기장대 발현 시스템을 직접 이용할 수 있고, 유전체 및 전사체 서열이 밝혀져 있어 유전체 편집 기술을 활용하여 식물 특이적 당 잔기를 제거할 수 있다. 현재까지 많은 식물 이용 바이오의약품 생산 연구가 진행되어왔지만 식물 세포의 낮은 단백질 생산성을 극복하고 생산 수율 증진시키는 데 한계가 있다. 이러한 생산기술의 한계를 극복하고 안전하고 저렴한 식물 기반의 차세대 biosimilar 생산 플랫폼을 개발하는 노력이 필요하다. 따라서 본 연구에서는 애기장대에서 소포체 스트레스에 의해 유발되는 미절첩 단백질 반응(unfolded protein response, UPR)의 핵심 신호전달 유전자를 유전체 수준에서 교정한 배추 변이체 후보군을 확보하고자 하였다. CRISPR/Cas9시스템과 Agrobacterium 형질전환을 통한 유전자 교정으로 배추 형질전환체를 획득하였다. PCR 분석을 통해 형질전환 여부를 확인한 후 deep-sequencing 분석을 수행하여 유전자 변이 개체를 선발하였으며, 자가수정을 통한 homozygous 계통을 확보하였다. 확보된 E1 종자를 파종하여 파종 7일 후 N-glycosylation inhibitor인 tunicamycin (TM)을 농도별로 처리하여 기존 식물(wild type)의 식물체와 유전자 교정이 된 식물체간의 스트레스 내성을 비교하였다. 세포사멸 조절인자가 제거된 불멸화(immortalization) 식물체 세포의 경우 wild type 세포에 비해 스트레스 저항성과 증식율(proliferation)이 증가할 것으로 기대되며 또한 식물 세포배양 시스템으로 전환 시 단백질 생산 수율도 증가될 것으로 기대된다.
생물검정을 이용한 딸기(Fragaria×ananassa Duch.) 유전자원의 역병균(Phytophthora cactorum)에 의한 crown rot 저항성 평가
이예린(Ye Rin Lee),장원석(Won Suk Jang),남명현(Myeong Hyeon Nam),이준대(Jundae Lee) 한국육종학회 2018 한국육종학회지 Vol.50 No.3
Crown rot caused by Phytophthora cactorum is one of several serious, widespread diseases that cause problems in strawberry (Fragaria × ananassa Duch.) cultivation. In this study, we aimed to identify a strawberry resistant to P. cactorum through bioassay. A total of 104 wild and cultivated strawberry accessions, including ‘Sulhyang’ and ‘Akihime’, were inoculated with an isolate of P. cactorum, ‘PC151111’. A zoospore suspension was used with a density of approximately 10⁶ zoosporesㆍmL⁻¹. Three strawberry seedlings were tested three times independently. The disease index was scored on a scale from 0-4 through symptom observation while the inoculated plants were incubated at 25±3°C under 16 h/8 h (light/dark) conditions for three weeks. The results showed that the mean disease index varied from 0.78-3.78, and the ‘Pechika’ and ‘Kaorino’ cultivars were highly resistant to P. cactorum, with mean disease indexes of 0.78 and 0.89, respectively. These cultivars will be useful resources in breeding strawberries resistant to crown rot.
이예린 ( Ye-rin Lee ),한경순 ( Gyeong-soon Han ) 한국치위생학회(구 한국치위생교육학회) 2020 한국치위생학회지 Vol.20 No.6
Objectives: The purpose of this study was to investigate the infection control system and actual conditions according to the type of dental medical institution. Methods: From April 1st to May 10th, 2020, dental institutions were recruited through stratified random sampling. Each item constituting the infection control system by dental institution type was analyzed using the chi-square test and fisher’s exact test. Results: The infection control system and operation level of each dental institution type was 89.0 points for university-general hospitals, 75.6 points for dental hospitals, and 34.4 points for dental clinics. The environment management levels were 76.1, 72.5, 73.0, and 74.0 points for university-general hospitals, similar to 77.2, 75.1, 71.0, and 73.8 points for dental hospitals, while dental clinics had 61.1, 40.0, 37.0, and 45.6 points. Prevention and management of staff infection exposure, wearing personal protective equipment, and hand hygiene levels were 90.7, 75.5, 88.5 points for university-general hospitals, 79.8, 79.5, 80.4 points for dental hospitals, and 50.2, 88.0, 61.5 points for dental clinics, respectively. Conclusions: Efforts are required to bring about improvement in the areas of insufficient infection control in order to raise the overall infection control levels, especially the management of dental clinics is urgently needed.