Anti-Inflammatory Effect for Atherosclerosis Progression by Sodium-Glucose Cotransporter 2 (SGLT-2) Inhibitor in a Normoglycemic Rabbit Model

이슬기,Seung-Jun Lee,Jung-Jae Lee,Jung-SunKim,Oh-Hyun Lee,김충기,Darae Kim,Yong-Ho Lee,JaeWonOh,Se Il Park,전옥희,Sung-Jin Hong,Chul-Min Ahn,Byeong-Keuk Kim,Young-Guk Ko,Donghoon Choi,Myeong-Ki Hong,Yangsoo Ja 대한심장학회 2020 Korean Circulation Journal Vol.50 No.5

Background and Objectives: We sought to investigate an anti-atherosclerotic and anti-inflammatory effect of sodium-glucose cotransporter-2 (SGLT-2) inhibitors in normoglycemic atherosclerotic rabbit model. Methods: Male New Zealand white rabbits (n=26) were fed with a 1% high-cholesterol diet for 7 weeks followed by normal diet for 2 weeks. After balloon catheter injury, the rabbits were administered with the Dapagliflozin (1mg/kg/day) or control-medium for 8 weeks (n=13 for each group). All lesions were assessed with angiography, optical coherence tomography (OCT), and histological assessment. Results: Atheroma burden (38.51±3.16% vs. 21.91±1.22%, p<0.01) and lipid accumulation (18.90±3.63% vs. 10.20±2.03%, p=0.047) was significantly decreased by SGLT-2 inhibitor treatment. The SGLT-2 inhibitor group showed lower macrophage infiltration (20.23±1.89% vs. 12.72±1.95%, p=0.01) as well as tumor necrosis factor (TNF)-α expression (31.17±4.40% vs. 19.47±2.10%, p=0.025). Relative area of inducible nitric oxide synthase+ macrophages was tended to be lower in the SGLT-2 inhibitor-treated group (1.00±0.16% vs. 0.71±0.10%, p=0.13), while relative proportion of Arg1+ macrophage was markedly increased (1.00±0.27% vs. 2.43±0.64%, p=0.04). As a result, progression of atherosclerosis was markedly attenuated in SGLT-2 inhibitor treated group (OCT area stenosis, 32.13±1.20% vs. 22.77±0.88%, p<0.01). Mechanistically, SGLT-2 treatment mitigated the inflammatory responses in macrophage. Especially, Toll-like receptor 4/nuclear factor-kappa B signaling pathway, and their downstream effectors such as interleukin-6 and TNF-α were markedly suppressed by SGLT-2 inhibitor treatment. Conclusions: These results together suggest that SGLT-2 inhibitor exerts an anti-atherosclerotic effect through favorable modulation of inflammatory response as well as macrophage characteristics in non-diabetic situation.

Genome-Wide Screen of Human Bromodomain-Containing Proteins Identifies Cecr2 as a Novel DNA Damage Response Protein

이슬기,권종범,박은정,이한새,Ye Seul Lee 한국분자세포생물학회 2012 Molecules and cells Vol.34 No.1

The formation of -H2AX foci after DNA double strand breaks (DSBs) is crucial for the cellular response to this lethal DNA damage. We previously have shown that BRG1, a chromatin remodeling enzyme, facilitates DSB repair by stimulating -H2AX formation, and this function of BRG1 requires the binding of BRGI to acetylated histone H3 on -H2AX-containing nucleosomes using its bromodomain (BRD), a protein module that specifically recognizes acetyl-Lys moieties. We also have shown that the BRD of BRG1, when ectopically expressed in cells, functions as a dominant negative inhibitor of the BRG1 activity to stimulate -H2AX and DSB repair. Here, we found that BRDs from a select group of proteins have no such activity, suggesting that the -H2AX inhibition activity of BRG1 BRD is specific. This finding led us to search for more BRDs that exhibit -H2AX inhibition activity in the hope of finding additional BRD-containing proteins involved in DNA damage responses. We screened a total of 52 individual BRDs present in 38 human BRD-containing proteins, comprising 93% of all human BRDs. We identified the BRD of cat eye syndrome chromosome region candidate 2 (Cecr2), which recently was shown to form a novel chromatin remodeling complex with unknown cellular functions, as having a strong -H2AX inhibition activity. This activity of Cecr2 BRD is specific because it depends on the chromatin binding affinity of Cecr2 BRD. Small interfering RNA knockdown experiments showed that Cecr2 is important for -H2AX formation and DSB repair. Therefore, our genome-wide screen identifies Cecr2 as a novel DNA damage response protein.

밭 비점오염저감을 위한 잔디초생대 적용 비용 및 활용성 평가

이슬기,장정렬,최경숙,Lee, Seul-Gi,Jang, Jeong-Ryeol,Choi, Kyung-Sook 한국농공학회 2015 한국농공학회논문집 Vol.57 No.2

This study was aimed to estimate the total application cost and utilization of Turf grass VFS application through the field experiment. The experimental plots were constructed in an upland field of Iksan city within the Saemangeum watershed. Turf grass was transplanted at the down-slope edge of the pollution source area in each of the treated plots. Three rainfall events were monitored during the experiment period, and the rainfall-runoff relationships and NPS pollution reduction effects of the VFS systems were assessed. As results, the reduction ratio of runoff volume ranged 14.1~64.0 %, while the NPS pollution reduction ratio ranged 9.8~73.9 % for SS, 24.0~84.2 % for T-N, 31.6~80.9 % for T-P respectively. The total cost of VFS application was estimated by considering purchase cost of Turf grass sods and construction and maintenance costs of VFS system as well as the loss caused by giving up crop cultivation for the area needed to construct the VFS. The total cost of the VFS was estimated to be approximately \3,379,000/ha/year for the first year of application, and this cost could be decreased to \1,899,000/ha/year from the second year as the construction cost of VFS could no longer need to be counted afterwards. Apart from the NPS pollution reduction effects, the possible utilization of VFS was examined by detaching Turf grass within 40 % of VFS area for sale during spring time when the VFS systems fully covered. The benefit of selling the detached Turf grass sods was estimated as \1,260,000/ha/year, and also found that the VFS area successfully recovered by the time of the summer period. This benefit could attract farmers to adopt the VFS technique to manage agricultural NPS pollution.

"황토를 이용한 건축재분야" 특허동향보고서

이슬기,Lee, Seul-Gi 한국특허정보원 2007 Patent 21 Vol.74 No.-

Chitosan-HNT 복합막을 이용한 기체 투과특성에 관한 연구

이슬기,홍세령,Lee, Seul Ki,Hong, Se Ryeong 한국막학회 2014 멤브레인 Vol.24 No.5

Chitosan에 HNT (halloysite nanotube)의 함량을 0, 3, 5, 10 wt%로 가하여 chitosan-HNT 복합막을 제조하였다. 복합막의 구조는 FT-IR, XRD, TGA, SEM으로 알아보았다. 기체투과 실험은 $30^{\circ}C$, $4kg/cm^2$ 조건에서 수행하였고, chitosan-HNT 복합막의 HNT 함량 변화에 따른 $CO_2$와 $CH_4$의 기체투과도와 선택도를 조사하였다. Chitosan-HNT 복합막의 기체 투과도는 HNT 함량이 3 wt%에서 가장 큰 값을 보였고, 그 이상의 함량에서는 감소하였다. 선택도($CO_2/N_2$)는 0~10 wt% 범위에서 HNT 내의 OH기와 $CO_2$ 간의 친화력으로 인하여 증가하였고, 약 1.3~3.8의 값을 보였다. Chitosan-HNT (halloysite nanotube) composite membranes were prepared by the addition of HNT 0, 3, 5, and 10 wt%. The structure of composite membranes were studied by FT-IR, XRD, TGA, and SEM. Gas permeation experiment were performed under condition of $30^{\circ}C$ and $4kgf/cm^2$. Gas permeability and selectivity were investigated by increasing the amount of HNT contents in the chitosan. Chitosan-HNT composite membrane for $CO_2$ and $CH_4$ showed the maximum value at 3 wt% of HNT content and decreased thereafter. The selectivity of ($CO_2/CH_4$) was increased due to its affinity with the OH groups on the HNT, was shown in the range of 1.3 to 3.8 at 0~10 wt%.

유전체 시대에 반수체 육종의 재발견

이슬기,김정선,강상호,손성한,원소윤,Lee, Seulki,Kim, Jung Sun,Kang, Sang-Ho,Sohn, Seong-Han,Won, So Youn 한국식물생명공학회 2016 식물생명공학회지 Vol.43 No.1

DNA 염기서열 분석기술의 진보는 많은 근본적인 생명현상을 이해하는데 기여해왔다. 유례없는 저비용에 염기서열을 대량으로 분석을 할 수 있게 되어 단일 규모의 실험실에서도 관심이 있는 종의 신규유전체를 해독할 수 있다. 게다가 유전집단의 전체 염기서열을 편향되지 않은 채 분석하여 무수한 분자마커를 발굴할 수 있게 됨에 따라 집단유전학 연구도 두드러지게 가속화되어 왔다. 그러나 식물의 유전체가 이형접합성, 반복염기서열, 배수성과 같은 복잡한 특성이 있다는 것을 고려해 볼 때 기술이 매우 빠르게 진화함에 따라 적절한 개체 혹은 집단을 확보하는 것이 식물 연구에서 주요한 문제가 되었다. 이러한 난제는 오래되었지만 매우 효율적인 기술인 반수체 육성을 통하여 극복될 수 있을 것이다. 정상적인 개체가 갖는 염색체의 절반을 보유하는 반수체 식물은 주로 자방이나 화분과 같은 배우체 세포를 배양함으로써 빠르게 구축될 수 있다. 뒤이은 반수체 식물의 염색체 배수화는 완벽한 동형접합성을 보이는 안정된 배가반수체를 만든다. 본 논문에서는 반수체 식물을 육성하고 판별하기 위한 고전적인 방법론을 요약할 것이다. 게다가 동원체의 히스톤을 후성적으로 조절함으로써 반수체를 유도하는 방법을 설명할 것이다. 마지막으로, 유전체 시대에 반수체 식물의 활용 방안을 유전체 해독과 집단 유전학의 측면에서 논의할 것이다. Advances in DNA sequencing technologies have contributed to revolutionary understanding of many fundamental biological processes. With unprecedented cost-effective and high-throughput sequencing, a single laboratory can afford to de novo sequence the whole genome for species of interest. In addition, population genetic studies have been remarkably accelerated by numerous molecular markers identified from unbiased genome-wide sequences of population samples. As sequencing technologies have evolved very rapidly, acquiring appropriate individual plants or populations is a major bottleneck in plant research considering the complex nature of plant genome, such as heterozygosity, repetitiveness, and polyploidy. This challenge could be overcome by the old but effective method known as haploid induction. Haploid plants containing half of their sporophytic chromosomes can be rapidly generated mainly by culturing gametophytic cells such as ovules or pollens. Subsequent chromosome doubling in haploid plants can generate stable doubled haploid (DH) with perfect homozygosity. Here, classical methodology to generate and identify haploid plants or DH are summarized. In addition, haploid induction by epigenetic regulation of centromeric histone is explained. Furthermore, the utilization of haploid plant in the genomics era is discussed in the aspect of genome sequencing project and population genetic studies.

Adaptive lasso를 이용한 희박벡터자기회귀모형에서의 변수 선택

이슬기,백창룡,Lee, Sl Gi,Baek, Changryong 한국통계학회 2016 응용통계연구 Vol.29 No.1

본 논문은 다차원의 시계열 자료 분석에서 효율적인 희박벡터자기회귀모형에서의 모수 추정에 대해서 연구한다. 희박벡터자기회귀모형은 영에 가까운 계수를 정확이 영으로 둠으로써 희박성을 확보한다. 따라서 변수 선택과 모수 추정을 한꺼번에 할 수 있는 lasso를 이용한 방법론을 희박벡터자기회귀모형의 추정에 쓸 수 있다. 하지만 Davis 등(2015)에서는 모의실험을 통해 일반적인 lasso의 경우 영이아닌 계수를 참값보다 훨씬 더 많이 찾아 희박성에 약점이 있음을 보고하였다. 이에 따라 본 연구는 희박벡터자기회귀모형에 adaptive lasso를 이용하면 일반 lasso보다 희박성을 비롯한 전반적인 모수의 추정이 매우 유의하게 개선됨을 보인다. 또한 adaptive lasso에서 쓰이는 튜닝 모수들에 대한 선택도 아울러 논의한다. This paper considers variable selection in the sparse vector autoregressive (sVAR) model where sparsity comes from setting small coefficients to exact zeros. In the estimation perspective, Davis et al. (2015) showed that the lasso type of regularization method is successful because it provides a simultaneous variable selection and parameter estimation even for time series data. However, their simulations study reports that the regular lasso overestimates the number of non-zero coefficients, hence its finite sample performance needs improvements. In this article, we show that the adaptive lasso significantly improves the performance where the adaptive lasso finds the sparsity patterns superior to the regular lasso. Some tuning parameter selections in the adaptive lasso are also discussed from the simulations study.

익산 밭경지 특성을 고려한 초생대 유출 및 유사 저감효과 모의

이슬기,장정렬,최경숙,Lee, Seul Gi,Jang, Jeong Ryeol,Choi, Kyung Sook 한국농공학회 2014 한국농공학회논문집 Vol.56 No.5

The goal of this study was to evaluate sediment reduction effects of VFS (vegetative filter strip) applied for Iksan area in Saemangeum watershed. This study simulated runoff and sediment load from different types of uplands using VFSMOD-W. The general upland characteristics of the study area was investigated to build reasonable scenarios of the simulation. The simulation scenarios were designed by various areas, shapes, and slopes of uplands. Grass mixture was selected as VFS vegetation and the size of VFS was fixed as 10 % of uplands area. Additionally 50mm, 100mm, 150mm of daily rainfall were applied for the runoff and sediment simulation. As results, the calculated runoff and sediment loads were obtained $20.7{\sim}1,030.6m^3$ and 568.4~675,731.4 kg for the range of 0.1~1.0 ha of uplands with 7 % and 15 % slopes. The reduction effects on runoff and sediment were obtained 5~10 % and 21.0~47.7 % respectively from VFS applications. The VFSMOD-W simulations showed that runoff tended to increase as upland area and amount of rainfall increased while sediment increased when slope, length and area of uplands and amount of rainfall increased. These results indicated that rainfall amount and upland size are the critical factors for the generation of runoff and sediment load. In order to support this conclusion, further studies such as, long term monitoring, field experiments, and to calibrate and evaluate the model are necessary.

새만금유역 밭경지 초생대 유사저감효과 모의

이슬기,장정렬,최경숙,Lee, Seul Gi,Jang, Jeong Ryeol,Choi, Kyung Sook 한국수자원학회 2018 한국수자원학회논문집 Vol.51 No.6

본 연구에서는 VFSMOD-W 모형을 활용하여 새만금 유역의 초생대 기법 적용에 따른 유사저감효과를 예측하고자 하였다. 먼저 모형의 신뢰도 확보를 위해 새만금 유역 내 초생대 실측치를 활용하여 매개변수를 보정하였으며, 새만금 유역 내 밭경지 특성을 파악하여 이를 토대로 모의 시나리오를 개발하였다. 다양한 밭경지 특성을 반영하기 위해 개발된 모의시나리오는 밭경지 규모 1 ha, 5 ha, 10 ha로 밭의 폭과 길이 비는 1 : 1를 적용하였으며, 밭의 경사는 7%, 15%를 고려하였다. 또한 강우조건은 50 mm, 100 mm, 150 mm, 200 mm 일강우량을 적용하였다. 그 결과, 초생대의 유출저감효과는 밭경사 7%와 15%에 대해 2.9~13.5%, 2.9~12.1%로 각각 나타났으며, 유사저감효과는 33.8~97.0%, 27.1~85.9%로 각각 나타나 유출저감효과에 비해 상대적으로 높은 저감효과를 나타내었다. 유출 및 유사 발생에 영향을 미치는 밭규모, 경사 및 강우량 인자에 따라 초생대 효과는 큰 차이를 나타내었다. 본 모의 결과를 바탕으로 새만금 유역내 밭경지 규모 10 ha 미만, 경사 15% 이내의 조건에서 밭면적 10% 규모로 초생대를 조성할 경우 유사저감 효과를 기대할 수 있을 것으로 사료된다. The study was intended to simulate the sediment reduction effects of the Vegetative Filter Strip (VFS) in uplands of Saemangeum watershed through VFSMOD-W model application. The model was calibrated by using the field data and the simulation scenarios were designed based on the investigation of uplands characteristics in Saemangeum watershed. The simulation scenarios were considered various size and slope of uplands including 1 ha, 5 ha, 10 ha of field size with width-length ratio of 1 : 1 having 7% and 15% of slopes under the daily rainfall of 50 mm, 100 mm, 150 mm, and 200 mm in order to mimic the different fields conditions. The effluent reduction ranged from 2.9~13.5% and 2.9~12.1% for runoff, and 33.8~97.0% and 27.1~85.9% for sediment under the field's slope of 7% and 15%, respectively. The VFS reduction effects showed different degree of influence from field size, slope, rainfall amounts. Based on the simulated results, the sediment contributing non-point source pollution expected to be reduced in the condition of VFS constructed 10% of fields in outlet of less than 10 ha of uplands having less than 15% of the slope.

BIM과 온톨로지를 활용한 표준내역항목 추론 자동화

이슬기,김가람,유정호,Lee, Seul-Ki,Kim, Ka-Ram,Yu, Jung-Ho 한국건설관리학회 2012 한국건설관리학회 논문집 Vol.13 No.3

BIM(Building Information Model) 기반으로 기본설계를 수행한 경우에도 개략적인 설계 정보만 제공되므로, 내역작성에 필요한 충분한 정보를 설계도면으로부터 확보하는 것이 어렵다. 하지만 대부분 BIM기반 공사비산정 관련 연구들은 물량산출 자동화 또는 BIM 기반 물량산출결과의 정확도 향상을 위한 방안 제시하는 것이 대부분이며, 건설사업의 공사비산정에서 요구되고 있는 표준품셈 및 일위대가에 대한 고려가 미흡하다. 따라서 본 연구에서는 BIM 활용의 장점을 활용하고 여기에 온톨로지 기술을 접목하여, BIM 기반의 기본설계 정보로부터 내역서 생성에 필요한 작업내역을 자동으로 추출하는 프로세스를 제시한다. 이 프로세스를 적용할 경우, BIM 정보의 활용성이 더욱 향상될 것으로 기대되며, 견적자의 자의적 판단이 개입되던 문제를 해결하여 동일한 BIM이라면 동일한 견적결과를 얻을 수 있는 일관성 있는 내역작성 방법 개발의 기초가 될 것으로 기대된다. The rough design information is only available from BIM(Building Information Model) based schematic design. So, it is difficult to obtain sufficient information for generating BOQ. Like 2D design, there are some problems that the results are depend on what the choice of cost estimator. However, the most research of BIM based cost estimation are focus on quantity takeoff, the consideration of work item for generating BOQ is insufficient. Therefore, this paper present automatic inference process of work items in a BOQ using ontology. The proposed process and ontology is validated through applying tiling construction. If the proposed process is utilized, it is expected the basis of developing generation method for consistent BOQ by resolving intervention of cost estimator's arbitrary decision.