$H_{2}O_2$와 A$\beta$로 유도된 pc12 cell에서 생산조인(生酸棗仁) 수추출물의 항산화 및 항치매 효과

이상원,김대현,윤종현,김진우,정은영,이성근,이기상,김태헌,류영수,강형원,Lee, Sang-Won,Kim, Dae-Hyun,Yun, Jong-Hyun,Kim, Jin-Woo,Jung, Ejun-Young,Lee, Seoung-Geun,Lee, Key-Sang,Kim, Tae-Heon,Lyu, Yeoung-Su,Kang, Hyung-Won 대한한방신경정신과학회 2008 동의신경정신과학회지 Vol.19 No.3

Objective: The antioxidant and anti-Alzheimer effects of Semen Ziziphi Spinosae (SZS) water extract against the amyloid beta peptide (1-42) or H202-induced oxidative damage and cell death were investigated in rat pheochromocytoma line PC 12. Methods: The cells were incubated with SZS water extract and oxidative damage-inducing materials, amyloid beta peptide (1-42) or H2O2 for 24 h. The cellular viability was assessed by WST-1 assay, cytotoxic damage by LDH activity assay, oxidative damages of cells by fluorescence spectrophotometric method, and apoptosis by TUNEL staining assay. Results and Conclusions: 1. Preincubation of the cells with SZS water extract prior to amyloid beta peptide (1-42) (2 uM) or H2O2 (30 uM) exposure elevated the cell survival close to the control and decreased the level of LDH activity and the fluorescence from the cell homogenates and TUNEL staining of the cells, compared to only amyloid beta peptide (1-42) (2 uM) or H2O2 (30 uM) treated conditions. 2. Our study suggests that Semen Ziziphi Spinosae (SZS) water extract has protective effects against amyloid beta peptide (1-42) or H2O2-induced cell toxicity through the antioxidation mechanism, which might be beneficial for the treatment of Alzheimer's disease.

레버 증폭 구조의 플렉서를 이용한 공초점 현미경의 개발

이상원,김위한,정영대,박민규,김지현,이상인,이호,Lee, Sang-Won,Kim, Wi-Han,Jung, Young-Dae,Park, Min-Kyu,Kim, Jee-Hyun,Lee, Sang-In,Lee, Ho 한국광학회 2011 한국광학회지 Vol.22 No.1

본 연구에서는 레버 증폭 구조를 이용한 플렉서 기반의 나노 스테이지를 설계, 제작하였다. 제작된 나노 스테이지를 샘플 스테이지로 채용한 공초점현미경을 개발하였다. 2차원 이미징의 구현을 위해서, 기존의 공초점현미경은 레이저를 이미징 평면상에 스캐닝하는 방식으로 구현된다. 본 연구에서는 백 나노미터의 구동정밀도를 가지는 나노 스테이지 위에 놓인 샘플을 2차원으로 스캐닝하면서 2차원 이미지를 구현할 수 있는 공초점현미경을 개발하였다. 플렉서 기반의 나노 스테이지는 이중 판스프링, 변위증폭 레버, PZT 엑추에이터 그리고 변위 센서로 구성 되어 있다. 스테이지의 구동 성능 해석을 위해 상용 유한요소 해석 프로그램을 이용하였다. 현미경에 사용되는 광원은 적색광 레이저이며, 레이저는 여러 광학요소를 거쳐 샘플스테이지의 샘플에 입사되고, 반사된 빛은 광센서인 PMT(Photo Multiplying Tube)로 계측되게 된다. 계측된 빛의 크기를 이용하여서 2차원 이미징을 구현하였다. 개발된 공초점 현미경으로 생쥐 귀의 피부조직을 관찰하여 현미경의 이미징 성능을 검증하였다. 설계된 샘플 스테이지는 기존의 공초점 현미경의 기계적인 Beam 스캐너를 대신함으로써 현미경의 광경로 및 전체시스템을 간소화 하였다. A confocal microscope was developed utilizing a scanning sample stage based on a home-built double-compound flexure guide. A scanning sample stage with nano-scale resolution consisted of a double leaf spring based flexure, a displacement amplifying lever, a Piezo-electric Transducer(PZT) actuator and capacitance sensors. The performance of the two-axis stage was analyzed using a commercial finite element method program prior to the implementation. A single line laser was employed as the light source along with the Photo Multiplier Tube(PMT) that served as the detector. The performance of the developed confocal microscope was evaluated with a mouse ear skin imaging test. The designed scanning stage enabled us to build the confocal microscope without the two optical scanning mirror modules that are essential in the conventional laser scanning confocal microscope. The elimination of the scanning mirror modules makes the optical design of the confocal microscope simpler and more compact than the conventional system.

경제적 관점에서 본 4대강 살리기 사업

이상원,Lee, Sang-Won 한국수자원학회 2009 물과 미래(한국수자원학회지) Vol.42 No.1

잡음환경에서 음성-영상 정보의 통합 처리를 사용한 숫자음 인식에 관한 연구

이상원,박인정,Lee, Sang-Won,Park, In-Jung 대한전자공학회 2001 電子工學會論文誌-CI (Computer and Information) Vol.38 No.3

본 논문에서는 한국어 숫자음 인식을 위해 음성과 영상 정보를 사용하고, 음성에 사용하는 선형예측계수 알고리즘을 영상에 적용하는 방법을 제안한다. 입력으로 얻어지는 음성신호는 0.95의 매개변수를 통해 고역 신호가 강조되고, 해밍창과 자기상관 분석, Levinson-Durbin 알고리즘에 의해 13차 선형예측계수를 구한다. 마찬가지로, 그레이 영상신호도, 음성의 자기상관 분석, Levinson-Durbin 알고리즘을 사용하여 13차의 2차원 선형예측계수를 구한다, 이러한 음성/영상 신호에 대한 선형예측계수들은 다층 신경회로망에 적용하여 학습이 이루어졌고, 각 레벨의 잡음이 섞인 음성신호를 적용한 결과, 숫자음 '3', '5', '9' 에서 음성만으로 인식한 결과보다 훨씬 좋은 인식결과를 얻을 수 있었다. 결과적으로, 본 연구에서는 영상 신호의 2차원 선형 예측 계수들이 음성인식에 사용될 경우, 특징 추출에 따른 부가적인 알고리즘이 새로 고안될 필요가 없이, 음성특징 계수를 추출하는 방법을 그대로 사용할 수 있으며, 또한 데이터량과 인식율이 잡음 환경에서 보다 향상되는 효율적인 방법을 제시하고 있음을 알 수 있었다. In this paper, a method that apply LP algorithm to image for speech recognition is suggested, using both speech and image information for recogniton of korean numeral speech. The input speech signal is pre-emphasized with parameter value 0.95, analyzed for B th LP coefficients using Hamming window, autocorrelation and Levinson-Durbin algorithm. Also, a gray image signal is analyzed for 2-dimensional LP coefficients using autocorrelation and Levinson-Durbin algorithm like speech. These parameters are used for input parameters of neural network using back-propagation algorithm. The recognition experiment was carried out at each noise level, three numeral speechs, '3','5', and '9' were enhanced. Thus, in case of recognizing speech with 2-dimensional LP parameters, it results in a high recognition rate, a low parameter size, and a simple algorithm with no additional feature extraction algorithm.

퇴행성 경추질환에서 전방경유 추간판 절제술 및 골유합술의 결과분석

이상원,송근성,Lee, Sang Weon,Song, Geun Sung 대한신경외과학회 2001 Journal of Korean neurosurgical society Vol.30 No.2

Objectives : We retrospectively studied the efficacy of anterior cervical discectomy and interbody fusion without plate fixation in degenerative cervical disease. Methods : Thirty two consecutive patients with degenerative cervical disease treated by decompression and interbody fusion(Smith-Robinson technique) without anterior cervical plating were studied for postoperative complication rate as well as the clinical and radiologic outcomes and were compared the result of ours with other reported series where the anterior cervical plating was used. Results : All cases were reviewed after average period of 13 months for the purpose of this study. There were 4 postoperative complications related to grafting. A solid fusion was obtained in all cases with single-level fusion(n=21) and 81.8 % of the cases with a two-level fusion(n=11). The overall fusion rate was 93.8 % and fusion rate per level fused was 95.3%. The clinical outcome of the patients was comparable with that in the literature, with one patient having a poor result. Comparing the result of this study with others of the anterior cervical plating, clinical outcome and fusion rate were not superior in plate fixation group in single-level fusion, but increased fusion rate and decreased graft-related complication rate were noted in multilevel fusion with plate fixation. However, the clinical outcome was not superior to noninstrumented fusion group of this study. Conclusion : These results demonstrate that anterior cervical discectomy and interbody fusion(Smith-Robinson technique) without instrumentation is safe and reliable method of single-level fusion in degenerative cervical disease. Plate fixation system doesn't seem necessary in single level fusion in degenerative cervical disease.

RAMAN 분광기를 이용한 유리의 기포 분석

이상원,Lee, Sangwon 한국분석과학회 1993 분석과학 Vol.6 No.2

Collins 내동기의 최적 설계조건

이상원,김수연,정평석,Lee, S.W.,Kim, S.Y.,Jung, P.S. 대한설비공학회 1992 설비공학 논문집 Vol.4 No.3

The Collins cryocooler is numerically analysed with the optimization technique, and the optimum operating and design conditions are searched. This paper shows that liquefied helium quantity has an external maximum w.r.t. the total mass flow rate, the mass flow rates through expander and the capacities of heat exchangers. The liquefied helium quantity increases as the compressor exit pressure of the cryocooler does. The maximum quantity of liquefied helium and the maximum coefficient of performance have been found to exist in extremum, depending on the ratios of each heat exchanger capicities to the total one. At the optimum condition, the capacity of heat exchanger in high temperature region is larger than that in low temperature region.

울산-언양 지역 화강암체 주변의 퇴적암류에 대한 접촉변성작용에 관한 연구

이상원,이준동,김정진,Lee, Sang Won,Lee, Joon Dong,Kim, Jeong Jin 대한자원환경지질학회 1992 자원환경지질 Vol.25 No.4

The study area consists of sedimentary and volcanic rocks of Gyeongsang Supergroup, granitoid intrusives, and hornfelses around the granitoids. Granitoid intrusives occur in small stocks in Nijeon-ri, Uggogri, and Yul-ri area. The masses in Nijeon-ri and Uggog-ri are hornblende-biotite granodiorite, biotite granodiorite respectively, and Yul-ri mass is biotite granite. Surrounding sediments of these masses were thermally metamorphosed and contact aureoles were formed. The studied granitoids are considered to be formed by sequential crystallization-differentiation from calc-alkalic granitoid magma. Metamorphic minerals occurring in contact aureole are chlorite, actinolite, epidote, and biotite. Diopside and hornblende are observed in small amount in some lithology around contact aureole. The lithology of contact aureole is predominantly silty and sandy, and characteristic metamorphic minerals were poorly developed because of low temperature metamorphism. Low temperature in contact aureole could be deduced from the facts that the intrusions were small size, shallow depth, low temperature, and rare movement of volatiles from magma.

반도체 공정용 플라즈마 발생장치의 종류와 특성

이상원,Lee Sang-Won 한국전기전자재료학회 2006 전기전자재료 Vol.19 No.6

재해예방사업의 한계와 개선방안 모색

이상원,Lee, Sang-Won 한국방재협회 2017 방재저널 Vol.19 No.4