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우리나라 양식 자주복, Takifugu rubripes (Temminck & Schlegel, 1850)에서 발생한 Vibrio atlanticus의 첫 감염 사례 보고
이남실,조미영,정승희,원경미,Lee, Nam-Sil,Cho, Miyoung,Jung, Sung-hee,Won, Kyoungmi 한국어병학회 2018 한국어병학회지 Vol.31 No.2
본 연구는 육상 순환 양식장, 약 $20^{\circ}C$의 수온에서 양식 중이던 자주복에서 체표에 백탁과 궤양을 보이는 개체가 다수 발생하여 그 원인을 밝히고자 하였다. TCBS 선택배지에서 순수 배양된 균체를 확인하고 계대 배양하여 16S rRNA와 rpoA 유전자의 염기서열 분석을 실시하였으며, 그 결과는 Vibrio atlanticus와 99.77%의 상동성을 가지는 것으로 나타났다. 생화학적 특징을 이미 보고된 같은 균종과 비교한 결과, Voges-Proskauer test와 Gelatin 가수분해능에 대한 결과에서 차이를 나타내는 것으로 확인하였다. A case of disease was occurred in tiger puffer, Takifugu rubripes, reared in circulated system (water temperature was about $20^{\circ}C$) on land in Korea. Diseased fish showed a unique external sign, the cloudy skin ulcer in various sizes. We investigated the cause of disease, and isolated one pure cultured bacterium, TP01. This had 99.7% homology with Vibrio atlanticus by sequence analysis of the 16S rRNA and rpoA genes. TP01 showed some differences with other reported V. atlanticus strains in the biochemical characteristics as like Voges-Proskauer test and gelatin hydrolysis.
단보 : 생약재 5종 열수추출물의 어류 병원체에 대한 in-vitro 약효와 선택된 한 종(황금)의 넙치에서의 항균효능과 독성시험
이남실 ( Nam Sil Lee ),정승희 ( Sung Hee Jeong ),지보영 ( Bo Young Jee ) 한국어병학회 2010 한국어병학회지 Vol.23 No.1
5가지 생약재로부터 얻어낸 열수추출물을 대상으로 시험관(in-vitro) 실험을 통하여 어류병원체에 대한 항세균, 항곰팡이, 항기생충 효과를 검토하였다. 황금의 효과가 가장 두드러졌으며, 황금의 열수추출물을 사료에 흡수시켜 넙치에 급여하고 4, 8, 12주 후에 다양한 생리학적 반응과 세균성질병(Edwardsiellosis, Streptococosis)에 대한 예방효과를 조사한 결과, 성장효과에 대한 변화 이외의 뚜렷한 생리학적변화는 나타나지 않았으며, 두 가지 세균성 질병에 대한 예방효과도 확인되지 않았다. 따라서 이 후 추가적인 조사·연구가 이루어져야 할 것으로 생각된다. Hot water extracts of 5 herbs were tested in-vitro for anti-bacterial, anti-fungal, anti-parasitical effects for possible use against fish diseases. Skullcap, Scutellaria baicalensis, was the most effective herb in these 5. The effects of skullcap in cultured flounder were examined for various physiological responses and bacterial disease-prevention follow as feeding skullcap absorbed diet for 4, 8, 12weeks. There were not any significant effects in physiological responses, except beneficial action of growth promotion. No definitive preventive activity was observed with experimental feeding of the extract against infected flounder. As we could not confirm in-vivo antibacterial effects of skullcap in flounder despite its in-vitro efficacy, further studies are needed to define the in-vivo efficacy.
제주지역 양식 넙치(Paralichthys olivaceus)의 점액포자충 감염조직에 대한 병리조직학적 관찰
이남실 ( Nam-sil Lee ),김아란 ( Aran Kim ),서한길 ( Han-gil Seo ),최혜승 ( He Sung Choi ),조미영 ( Miyoung Cho ) 한국수산과학회 2021 한국수산과학회지 Vol.54 No.5
In recent years, myxosporean infection from the cultured olive flounders Paralichthys olivaceus, have been frequently observed in Jeju island, South Korea. This study aimed to compare histopathological and molecular-biological methods of examining myxosporean infection from these flounders. Samples were obtained from affected individuals exhibiting emaciation or abdominal distention and a polymerase chain reaction (PCR) indicative of Parvicapsular anisocaudata, Enteromyxum leei and Kudoa septempunctata were initiated. Histopathological examination were conducted with H&E stained tissue sections, and then in-situ hybridization (ISH) reaction were processed with selected sections using P. anisocaudata, E. leei, K. septempunctata and Scuticociliate probes. Renal and intestinal tissue degeneration were common symptoms associated with all samples. Sever glomerular and renal tubular degeneration were evident, as were intestinal epithelial desquamation and spore formation in the epithelial cells. The results of conventional PCR analysis and ISH reactions revealed differences, and we suspect that various microparasites may have been associated with the symptoms manifested.
해수 순치 중의 뱀장어, Anguilla japonica에서 나타나는 Vibrio scophthalmi 감염증
이남실 ( Nam Sil Lee ),김대중 ( Dae Jung Kim ),이배익 ( Be Ik Lee ),김신권 ( Sin Kwon Kim ),김명석 ( Myung Suk Kim ),김이청 ( Yi Cheong Kim ) 한국어병학회 2012 한국어병학회지 Vol.25 No.3
Vibriosis is one of the most prevalent fish disease belonging to the genus Vibrio. In present study, Vibrio sp. isolated from Japanese eel was confirmed as Vibrio scophthalmi using analysis of the genomic sequence of 16S rRNA. The major signs were hemorrhage of body surface and inner surface of abdomen, severe enteritis and retention of ascitis. Histopathological examination revealed blood cell degenerations in various organs (gills, liver, spleen, kidney, heart, intestine), exfoliate of intestinal epithelium, and congestion and hemorrhage in intestinal lamina propria. This is the first case report on V. scophthalmi infection in Japanese eel Anguilla japonica.
PCR 기법을 이용한 2009년 우리나라 서해안과 남해안 바지락, Ruditapes philippinarum의 Perkinsus olseni 감염에 관한 보고
이남실 ( Nam Sil Lee ),황지연 ( Jee Youn Hwang ),최동림 ( Dong Lim Choi ),박명애 ( Myoung Ae Park ) 한국어병학회 2010 한국어병학회지 Vol.23 No.2
Prevalence of a protozoan parasite Perkinsus olseni in Manila clam Ruditapes philippinarum was surveyed from July to December 2009 on the west and south coast of Korea. P.olseni infection was diagnosed using two primer sets, P.olseni NTS Forward/P.olseni NTS Reverse set and PolsITS-140F/PolsITS-600R set in polymerase chain reaction(PCR). The results using PolsITS-140F and PolsITS-600R primer set was retained up to 60% at all stations from July to December, except for Padori. Especially, Goheung showed 100% prevalence from October to December. The results about comparison of the 4 station`s DNA sequences which were analyzed from PCR products(457bp) using PolsITS-140F and PolsITS-600R primer set, there were only 2base differences at Sunjedo.